Matthiessen, Birte; Hattich, Giannina S I; Pulina, Silvia; Hansen, Thomas; Reusch, Thorsten B H; Hamer, Jorin (2023): Long-term experiment (182 days) with the phytoplankton species Chaetoceros affinis and Emiliania huxleyi at three nutrient regimes: measurements of cell size and density [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.957873, In: Matthiessen, B et al. (2023): Experiments on coexisting phytoplankton species Chaetoceros affinis and Emiliania huxleyi in response to nutrients [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.957930
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Abstract:
Measurements of cell size, cell density, and nutrient concentration in a long-term experiment (182 days) with the marine phytoplankton species Chaetoceros affinis and Emiliania huxleyi, each consisting of nine genotypes (http://roscoff-culture-collection.org/; Emiliania huxleyi: GC22: RCC6383, C42: RCC6376, C41: RCC6375, C47: RCC4546, C96: RCC6381, C91: RCC6380, C35: RCC6374, C30: RCC6371, C48: RCC6377, Chaetoceros affinis: B63: RCC6347, B67: RCC6348, B68: RCC6349, B74: RCC6350). The species were cultivated together at three different nutrient regimes (10 N, 20 N, 30 N) with increasing nitrate supply. Nutrient concentrations were added as 9.0 ± 0.7 (10 N), 19.6 ± 0.8 (20 N), or 29.9 ± 0.9 μmoL-1 (30 N) nitrate and 0.9 ± 0.1 μmoL-1 phosphate. Silicate concentrations were aligned to reflect a 4:1 N:Si ratio. Cultivation was done in a semi-continuous batch cycle system with transfer of 2.88e7 ± 1.57e7 µm³ biovolume into a new batch every 7 days. Prior to the experimental start, genotypes were separately acclimated to experimental conditions with 20 μmol L−1 nitrate for 7 days. Cell density and cell volume were measured at the end of each batch cycle for both species. Phosphate, Nitrate and Nitrite were measured at the end of batch cycles 1, 7, 13, and 26.
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# | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
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1 | Type of study | Study type | Matthiessen, Birte | |||
2 | Sampling date/time, experiment | Date/time sampling exp | Matthiessen, Birte | |||
3 | Day of experiment | DOE | day | Matthiessen, Birte | ||
4 | Batch | Batch | Matthiessen, Birte | |||
5 | Bottle number | Bottle | Matthiessen, Birte | |||
6 | Treatment: nutrients | T:nutrients | Matthiessen, Birte | Nutrient regime | ||
7 | Morphotype | Morphotype | Matthiessen, Birte | based on cell diameter of Chaetoceros affinis, small (<6µm), medium(6-12 µm), large(>12 µm) | ||
8 | Emiliania huxleyi | E. huxleyi | #/ml | Matthiessen, Birte | Flow cytometer, Beckman Coulter, Gallios | |
9 | Emiliania huxleyi, cell biovolume | E. huxleyi cell biovol | µm3 | Matthiessen, Birte | ||
10 | Emiliania huxleyi, biovolume | E. huxleyi biovol | µm3/ml | Matthiessen, Birte | ||
11 | Chaetoceros affinis | C. affinis | #/ml | Matthiessen, Birte | Microscopy, Zeiss, Axiovert 200 and Axio Observer Z1 | |
12 | Chaetoceros affinis, cell biovolume | C. affinis cell biovol | µm3 | Matthiessen, Birte | ||
13 | Chaetoceros affinis, biovolume | C. affinis biovol | µm3/ml | Matthiessen, Birte | ||
14 | Biovolume | Biovol | µm3/ml | Matthiessen, Birte | total | |
15 | Chaetoceros affinis, height | C. affinis h | µm | Matthiessen, Birte | Microscopy, Zeiss, Axiovert 200 and Axio Observer Z1 | |
16 | Chaetoceros affinis, diameter | C. affinis diam | µm | Matthiessen, Birte | Microscopy, Zeiss, Axiovert 200 and Axio Observer Z1 | |
17 | Emiliania huxleyi, diameter | E. huxleyi diam | µm | Matthiessen, Birte | Microscopy, Zeiss, Axiovert 200 and Axio Observer Z1 | |
18 | Phosphate | [PO4]3- | µmol/l | Matthiessen, Birte | SEAL Analytical, continuous flow analyser QuAAtro | |
19 | Nitrite | [NO2]- | µmol/l | Matthiessen, Birte | SEAL Analytical, continuous flow analyser QuAAtro | |
20 | Nitrate | [NO3]- | µmol/l | Matthiessen, Birte | SEAL Analytical, continuous flow analyser QuAAtro |
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Creative Commons Attribution 4.0 International (CC-BY-4.0)
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Curation Level: Enhanced curation (CurationLevelC)
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11673 data points
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