Citation:

PANGAEA.957930

Name: Long-term experiment (182 days) with the phytoplankton species Chaetoceros affinis and Emiliania huxleyi at three nutrient regimes: measurements of cell size and density
License: https://creativecommons.org/licenses/by/4.0/
Keywords: cell size; diatoms; evolution; Genotype Sorting; Laboratory experiment; nutrients; phenotypic plasticity; Phytoplankton; Species Sorting; trait variability

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Abstract:

Measurements of cell size, cell density, and nutrient concentration in a long-term experiment (182 days) with the marine phytoplankton species Chaetoceros affinis and Emiliania huxleyi, each consisting of nine genotypes (http://roscoff-culture-collection.org/; Emiliania huxleyi: GC22: RCC6383, C42: RCC6376, C41: RCC6375, C47: RCC4546, C96: RCC6381, C91: RCC6380, C35: RCC6374, C30: RCC6371, C48: RCC6377, Chaetoceros affinis: B63: RCC6347, B67: RCC6348, B68: RCC6349, B74: RCC6350). The species were cultivated together at three different nutrient regimes (10 N, 20 N, 30 N) with increasing nitrate supply. Nutrient concentrations were added as 9.0 ± 0.7 (10 N), 19.6 ± 0.8 (20 N), or 29.9 ± 0.9 μmoL-1 (30 N) nitrate and 0.9 ± 0.1 μmoL-1 phosphate. Silicate concentrations were aligned to reflect a 4:1 N:Si ratio. Cultivation was done in a semi-continuous batch cycle system with transfer of 2.88e7 ± 1.57e7 µm³ biovolume into a new batch every 7 days. Prior to the experimental start, genotypes were separately acclimated to experimental conditions with 20 μmol L−1 nitrate for 7 days. Cell density and cell volume were measured at the end of each batch cycle for both species. Phosphate, Nitrate and Nitrite were measured at the end of batch cycles 1, 7, 13, and 26.

Keyword(s):

cell size; diatoms; evolution; Genotype Sorting; Laboratory experiment; nutrients; phenotypic plasticity; Phytoplankton; Species Sorting; trait variability

Project(s):

Priority Programme 1704 DynaTrait: Flexibility Matters: Interplay between Trait Diversity and Ecological Dynamics Using Aquatic Communities as Model Systems (SPP1704)

Funding:

German Research Foundation (DFG), grant/award no. MA5058/2-2

German Research Foundation (DFG), grant/award no. RE1708/17-2

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Matthiessen, Birte
2	Sampling date/time, experiment	Date/time sampling exp		Matthiessen, Birte
3	Day of experiment	DOE	day	Matthiessen, Birte
4	Batch	Batch		Matthiessen, Birte
5	Bottle number	Bottle		Matthiessen, Birte
6	Treatment: nutrients	T:nutrients		Matthiessen, Birte		Nutrient regime
7	Morphotype	Morphotype		Matthiessen, Birte		based on cell diameter of Chaetoceros affinis, small (<6µm), medium(6-12 µm), large(>12 µm)
8	Emiliania huxleyi	E. huxleyi	#/ml	Matthiessen, Birte	Flow cytometer, Beckman Coulter, Gallios
9	Emiliania huxleyi, cell biovolume	E. huxleyi cell biovol	µm³	Matthiessen, Birte
10	Emiliania huxleyi, biovolume	E. huxleyi biovol	µm³/ml	Matthiessen, Birte
11	Chaetoceros affinis	C. affinis	#/ml	Matthiessen, Birte	Microscopy, Zeiss, Axiovert 200 and Axio Observer Z1
12	Chaetoceros affinis, cell biovolume	C. affinis cell biovol	µm³	Matthiessen, Birte
13	Chaetoceros affinis, biovolume	C. affinis biovol	µm³/ml	Matthiessen, Birte
14	Biovolume	Biovol	µm³/ml	Matthiessen, Birte		total
15	Chaetoceros affinis, height	C. affinis h	µm	Matthiessen, Birte	Microscopy, Zeiss, Axiovert 200 and Axio Observer Z1
16	Chaetoceros affinis, diameter	C. affinis diam	µm	Matthiessen, Birte	Microscopy, Zeiss, Axiovert 200 and Axio Observer Z1
17	Emiliania huxleyi, diameter	E. huxleyi diam	µm	Matthiessen, Birte	Microscopy, Zeiss, Axiovert 200 and Axio Observer Z1
18	Phosphate	[PO4]3-	µmol/l	Matthiessen, Birte	SEAL Analytical, continuous flow analyser QuAAtro
19	Nitrite	[NO2]-	µmol/l	Matthiessen, Birte	SEAL Analytical, continuous flow analyser QuAAtro
20	Nitrate	[NO3]-	µmol/l	Matthiessen, Birte	SEAL Analytical, continuous flow analyser QuAAtro

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

11673 data points

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