Servetto, Natalia; Ruiz, Micaela Belen; Martinez, Mariano; Harms, Lars; de Aranzamendi, M C; Alurralde, Gastón; Giménez, D; Abele, Doris; Held, Christoph; Sahade, Ricardo José (2023): Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Apoptotic activity [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.956183
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Abstract:
Southern Ocean organisms are thought to be particularly vulnerable to ocean acidification, as they inhabit cold waters where calcite-aragonite saturation states are naturally low. It is also generally assumed that calcifying animals would be more affected by ocean acidification than non-calcifying ones. In this context, we aimed to study the impacts of reduced pH on the ascidia Cnemidocarpa verrucosa sp. A. Here, we used gene expression profiling and enzymatic activity to study the responses of that Antarctic benthic species to ocean acidification.
We sampled Cnemidocarpa verrucosa sp. A. by scuba diving at approximately 15 m depth at Carlini station, Potter Cove, King George Island, Antarctica.
Superoxide dismutase (SOD) activity was measured in the ascidia, samples (approximately 70 mg of brachial basket) were homogenized in 20 mM Tris-HCl, 1 mM EDTA, pH 7.6, with a ratio 1:4 w/v. Homogenates were centrifuged at 14,000 x g for 3 min at 4°C and the supernatant was used to measure SOD activity at 20°C following Livingstone et al. (1992) protocol. Supernatant was mixed with the measurement buffer (43 mM K₂HPO₄, 43 mM KH₂PO₄, 0.1 mM EDTA, pH 7.68), 5 mM Xanthina (Sigma X-0626), 100 µM Citocromo-C (Sigma C-2037), 0.3 mU/µl XOD (Xanthin-Oxidasa, Sigma X-4875) in 2 M (NH₄)2SO₄. The measurement was made in a photometer at 20°C, 550 nm wavelength, for 3 minutes, every 10 seconds. For the calculations, the total protein content of the samples was measured using the method of Bradford (1976). Superoxide dismutase activity was expressed in activity in the extract (mU) / amount of protein (mg). All measurements were made in triplicate.
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Related to:
Servetto, Natalia; Ruiz, Micaela Belen; Martinez, Mariano; Harms, Lars; de Aranzamendi, M C; Alurralde, Gastón; Giménez, D; Abele, Doris; Held, Christoph; Sahade, Ricardo José (2023): Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Superoxide dismutase activity. PANGAEA, https://doi.org/10.1594/PANGAEA.956185
References:
Bradford, Marion M (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2), 248-254, https://doi.org/10.1016/0003-2697(76)90527-3
Livingstone, D R; Lips, F; Garcia Martinez, P; Pipe, R K (1992): Antioxidant enzymes in the digestive gland of the common mussel Mytilus edulis. Marine Biology, 112, 265-276, https://doi.org/10.1007/BF00702471
Funding:
Coverage:
Latitude: -62.233333 * Longitude: -58.666667
Date/Time Start: 2015-12-16T00:00:00 * Date/Time End: 2015-12-16T00:00:00
Minimum Elevation: -15.0 m * Maximum Elevation: -15.0 m
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Servetto, Natalia | |||
| 2 | Sample code/label | Sample label | Servetto, Natalia | |||
| 3 | Type of study | Study type | Servetto, Natalia | |||
| 4 | Date/time start, experiment | Date/time start exp | Servetto, Natalia | |||
| 5 | Date/time end, experiment | Date/time end exp | Servetto, Natalia | |||
| 6 | Species | Species | Servetto, Natalia | |||
| 7 | Sample code/label | Sample label | Servetto, Natalia | |||
| 8 | Treatment | Treat | Servetto, Natalia | pH | ||
| 9 | Temperature, water | Temp | °C | Servetto, Natalia | ||
| 10 | Apoptotic activity, per protein | Apoptotic act/prot | RLU/µg | Servetto, Natalia | Spectrophotometer UV/Vis, Beckman Coulter, DU800 | Replicate 1; at 20ºC, 550 nm wavelength, for 3 minutes, every 10 seconds |
| 11 | Apoptotic activity, per protein | Apoptotic act/prot | RLU/µg | Servetto, Natalia | Spectrophotometer UV/Vis, Beckman Coulter, DU800 | Replicate 2; at 20ºC, 550 nm wavelength, for 3 minutes, every 10 seconds |
| 12 | Apoptotic activity, per protein | Apoptotic act/prot | RLU/µg | Servetto, Natalia | Spectrophotometer UV/Vis, Beckman Coulter, DU800 | Replicate 3; at 20ºC, 550 nm wavelength, for 3 minutes, every 10 seconds |
| 13 | Apoptotic activity, per protein | Apoptotic act/prot | RLU/µg | Servetto, Natalia | Calculated average/mean values | |
| 14 | Apoptotic activity, per protein | Apoptotic act/prot | RLU/µg | Servetto, Natalia | Spectrophotometer UV/Vis, Beckman Coulter, DU800 | Background/Blank without sample |
| 15 | Apoptotic activity, per protein | Apoptotic act/prot | RLU/µg | Servetto, Natalia | Background corrected |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
476 data points