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Servetto, Natalia; Ruiz, Micaela Belen; Martinez, Mariano; Harms, Lars; de Aranzamendi, M C; Alurralde, Gastón; Giménez, D; Abele, Doris; Held, Christoph; Sahade, Ricardo José (2023): Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Apoptotic activity [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.956183

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Abstract:
Southern Ocean organisms are thought to be particularly vulnerable to ocean acidification, as they inhabit cold waters where calcite-aragonite saturation states are naturally low. It is also generally assumed that calcifying animals would be more affected by ocean acidification than non-calcifying ones. In this context, we aimed to study the impacts of reduced pH on the ascidia Cnemidocarpa verrucosa sp. A. Here, we used gene expression profiling and enzymatic activity to study the responses of that Antarctic benthic species to ocean acidification.
We sampled Cnemidocarpa verrucosa sp. A. by scuba diving at approximately 15 m depth at Carlini station, Potter Cove, King George Island, Antarctica.
Superoxide dismutase (SOD) activity was measured in the ascidia, samples (approximately 70 mg of brachial basket) were homogenized in 20 mM Tris-HCl, 1 mM EDTA, pH 7.6, with a ratio 1:4 w/v. Homogenates were centrifuged at 14,000 x g for 3 min at 4°C and the supernatant was used to measure SOD activity at 20°C following Livingstone et al. (1992) protocol. Supernatant was mixed with the measurement buffer (43 mM K₂HPO₄, 43 mM KH₂PO₄, 0.1 mM EDTA, pH 7.68), 5 mM Xanthina (Sigma X-0626), 100 µM Citocromo-C (Sigma C-2037), 0.3 mU/µl XOD (Xanthin-Oxidasa, Sigma X-4875) in 2 M (NH₄)2SO₄. The measurement was made in a photometer at 20°C, 550 nm wavelength, for 3 minutes, every 10 seconds. For the calculations, the total protein content of the samples was measured using the method of Bradford (1976). Superoxide dismutase activity was expressed in activity in the extract (mU) / amount of protein (mg). All measurements were made in triplicate.
Keyword(s):
Antarctica; apoptosis; Caspase; Cnemidocarpa verrucosa sp. A; laboratory study; Potter Cove; Superoxide Dismutase; Tunicata
Related to:
Servetto, Natalia; Ruiz, Micaela Belen; Martinez, Mariano; Harms, Lars; de Aranzamendi, M C; Alurralde, Gastón; Giménez, D; Abele, Doris; Held, Christoph; Sahade, Ricardo José (2023): Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Superoxide dismutase activity. PANGAEA, https://doi.org/10.1594/PANGAEA.956185
References:
Bradford, Marion M (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2), 248-254, https://doi.org/10.1016/0003-2697(76)90527-3
Livingstone, D R; Lips, F; Garcia Martinez, P; Pipe, R K (1992): Antioxidant enzymes in the digestive gland of the common mussel Mytilus edulis. Marine Biology, 112, 265-276, https://doi.org/10.1007/BF00702471
Funding:
PADI Foundation (PADI), grant/award no. 47476
Coverage:
Latitude: -62.233333 * Longitude: -58.666667
Date/Time Start: 2015-12-16T00:00:00 * Date/Time End: 2015-12-16T00:00:00
Minimum Elevation: -15.0 m * Maximum Elevation: -15.0 m
Event(s):
Ant_PotterCove_2015  * Latitude: -62.233333 * Longitude: -58.666667 * Date/Time: 2015-12-16T00:00:00 * Elevation: -15.0 m * Location: Potter Cove, King George Island, Antarctic Peninsula * Method/Device: Sampling by diver (DIVER)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 Event labelEventServetto, Natalia
2 Sample code/labelSample labelServetto, Natalia
3 Type of studyStudy typeServetto, Natalia
4 Date/time start, experimentDate/time start expServetto, Natalia
5 Date/time end, experimentDate/time end expServetto, Natalia
6 SpeciesSpeciesServetto, Natalia
7 Sample code/labelSample labelServetto, Natalia
8 TreatmentTreatServetto, NataliapH
9 Temperature, waterTemp°CServetto, Natalia
10 Apoptotic activity, per proteinApoptotic act/protRLU/µgServetto, NataliaSpectrophotometer UV/Vis, Beckman Coulter, DU800Replicate 1; at 20ºC, 550 nm wavelength, for 3 minutes, every 10 seconds
11 Apoptotic activity, per proteinApoptotic act/protRLU/µgServetto, NataliaSpectrophotometer UV/Vis, Beckman Coulter, DU800Replicate 2; at 20ºC, 550 nm wavelength, for 3 minutes, every 10 seconds
12 Apoptotic activity, per proteinApoptotic act/protRLU/µgServetto, NataliaSpectrophotometer UV/Vis, Beckman Coulter, DU800Replicate 3; at 20ºC, 550 nm wavelength, for 3 minutes, every 10 seconds
13 Apoptotic activity, per proteinApoptotic act/protRLU/µgServetto, NataliaCalculated average/mean values
14 Apoptotic activity, per proteinApoptotic act/protRLU/µgServetto, NataliaSpectrophotometer UV/Vis, Beckman Coulter, DU800Background/Blank without sample
15 Apoptotic activity, per proteinApoptotic act/protRLU/µgServetto, NataliaBackground corrected
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
476 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Event		2 
Sample label		3 
Study type		4 
Date/time start exp		5 
Date/time end exp		6 
Species		7 
Sample label		8 
Treat
(pH)		9 
Temp [°C]		10 
Apoptotic act/prot [RLU/µg]
(Replicate 1; at 20ºC, 550 nm ...)		11 
Apoptotic act/prot [RLU/µg]
(Replicate 2; at 20ºC, 550 nm ...)		12 
Apoptotic act/prot [RLU/µg]
(Replicate 3; at 20ºC, 550 nm ...)		13 
Apoptotic act/prot [RLU/µg]
(Calculated average/mean values)		14 
Apoptotic act/prot [RLU/µg]
(Background/Blank without samp...)		15 
Apoptotic act/prot [RLU/µg]
(Background corrected)
Ant_PotterCove_2015 50_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTo cv8Initial time?1.0231328742294249410581436
Ant_PotterCove_201555_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTo cv3Initial time?1.0139522102172219110581133
Ant_PotterCove_201520_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTi_c_cv8control?1.0683577156695708222754807
Ant_PotterCove_201549_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTi_c_Cv7control?1.016391639167616511058593
Ant_PotterCove_201551_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTi_c_cv5control?1.0334323782256231710581259
Ant_PotterCove_201537_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTi_a_cv7acid?1.0301527712846287714611416
Ant_PotterCove_201546_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTi_a_cv6acid?1.0265927813979272012081512
Ant_PotterCove_201557_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTi_a_cv5acid?1.0349224062359238210581324
Ant_PotterCove_201560_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTi_a_cv4acid?1.018072013189119041058846
Ant_PotterCove_201524_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaT10_c_3control?1.0323073693829353022751254
Ant_PotterCove_201533_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaT10_c_cv9control?1.018451845195718821517365
Ant_PotterCove_201535_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaT10_c_cv4control?1.0294028372350270915171192
Ant_PotterCove_201517_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaT10_a_cv8acid?1.0469171354204444822752172
Ant_PotterCove_201519_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaT10_a_cv4acid?1.024342762219124622275187
Ant_PotterCove_201522_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaT10_a_cv5acid?1.0650769576545667022754395
Ant_PotterCove_201552_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaT10_a_cv10acid?1.0352033613623350110582443
Ant_PotterCove_201553_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaT10_a_cv6acid?1.0250923222200234410581286
Ant_PotterCove_201558_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaT10_a_cv9acid?1.012551442163914451058387
Ant_PotterCove_201559_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaT10_a_cv2acid?1.012171245144213011058243
Ant_PotterCove_201529_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTf_c_cv5control?1.0751861425824649515174978
Ant_PotterCove_201530_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTf_c_cv4control?1.0897097479148928815177771
Ant_PotterCove_201531_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTf_c_cv7control?1.0386733243989372715172210
Ant_PotterCove_201534_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTf_c_cv6control?1.0522553375037520015173683
Ant_PotterCove_201538_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTf_c_cv3control?1.01002812809101031098014619519
Ant_PotterCove_201547_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTf_c_cv9control?1.0436342133698409113952696
Ant_PotterCove_201527_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTf_a_cv1acid?1.0483143445927458827621826
Ant_PotterCove_201516_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTf_a_cv2acid?1.029896290912982129603227527328
Ant_PotterCove_201521_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTf_a_cv10acid?1.050963508974957750479227548204
Ant_PotterCove_201523_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTf_a_cv7acid?1.035495398213344536254227533979
Ant_PotterCove_201525_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTf_a_cv5acid?1.015140148031402614656227512381
Ant_PotterCove_201528_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTf_a_cv6acid?1.012443176021426914771151713254
Ant_PotterCove_201548_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTf_a_cv8control?1.0341741953464369213952297
Ant_PotterCove_201554_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTf_a_cv4acid?1.024353242882482124487105823429
Ant_PotterCove_201556_CASPlaboratory experiment2015-12-16T00:002016-02-19T00:00Cnemidocarpa verrucosaTf_a_cv1acid?1.020131957204120041058946