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Page, Tessa M; McDougall, Carmel; Bar, Ido; Diaz-Pulido, Guillermo (2022): Seawater carbonate chemistry in the experiment of transcriptomic responses of coralline algae to global change stressors [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.955746

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Abstract:
Crustose coralline algae (CCA) are calcifying red macroalgae that play important ecological roles including stabilisation of reef frameworks and provision of settlement cues for a range of marine invertebrates. Previous research into the responses of CCA to ocean warming (OW) and ocean acidification (OA) have found magnitude of effect to be species-specific. Response to OW and OA could be linked to divergent underlying molecular processes across species. Here we show Sporolithon durum, a species that exhibits low sensitivity to climate stressors, had little change in metabolic performance and did not significantly alter the expression of any genes when exposed to temperature and pH perturbations. In contrast, Porolithon onkodes, a major coral reef builder, reduced photosynthetic rates and had a labile transcriptomic response with over 400 significantly differentially expressed genes, with differential regulation of genes relating to physiological processes such as carbon acquisition and metabolism. The differential gene expression detected in P. onkodes implicates possible key metabolic pathways, including the pentose phosphate pathway, in the stress response of this species. We suggest S. durum is more resistant to OW and OA than P. onkodes, which demonstrated a high sensitivity to climate stressors and may have limited ability for acclimatisation. Understanding changes in gene expression in relation to physiological processes of CCA could help us understand and predict how different species will respond to, and persist in, future ocean conditions predicted for 2100.
Keyword(s):
Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Gene expression (incl. proteomics); Laboratory experiment; Macroalgae; Plantae; Porolithon onkodes; Rhodophyta; Single species; South Pacific; Sporolithon durum; Temperature; Tropical
Related to:
Page, Tessa M; McDougall, Carmel; Bar, Ido; Diaz-Pulido, Guillermo (2022): Transcriptomic stability or lability explains sensitivity to climate stressors in coralline algae. BMC Genomics, 23(1), 729, https://doi.org/10.1186/s12864-022-08931-9
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html
Page, Tessa M; McDougall, Carmel (2022): Transcriptomic responses of coralline algae to global change stressors. Open Science Framework, https://doi.org/10.17605/OSF.IO/2NKR4
Page, Tessa M; McDougall, Carmel; Bar, Ido; Diaz-Pulido, Guillermo (2022): Transcriptomic responses of coralline algae. Gene Expression Omnibus of the National Center for Biotechnology Information, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE211882
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-02-16.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypePage, Tessa MStudy
2TreatmentTreatPage, Tessa M
3SalinitySalPage, Tessa M
4Salinity, standard errorSal std e±Page, Tessa M
5Temperature, waterTemp°CPage, Tessa M
6Temperature, water, standard errorT std e±Page, Tessa M
7pHpHPage, Tessa MPotentiometrictotal scale
8pH, standard errorpH std e±Page, Tessa MPotentiometrictotal scale
9Alkalinity, totalATµmol/kgPage, Tessa MPotentiometric titration
10Alkalinity, total, standard errorAT std e±Page, Tessa MPotentiometric titration
11Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmPage, Tessa MCalculated using seacarb
12Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Page, Tessa MCalculated using seacarb
13Bicarbonate ion[HCO3]-µmol/kgPage, Tessa MCalculated using seacarb
14Bicarbonate ion, standard error[HCO3]- std e±Page, Tessa MCalculated using seacarb
15Carbonate ion[CO3]2-µmol/kgPage, Tessa MCalculated using seacarb
16Carbonate ion, standard error[CO3]2- std e±Page, Tessa MCalculated using seacarb
17High magnesium calcite saturation stateOmega HMCPage, Tessa MCalculated using seacarb
18High magnesium calcite saturation state, standard errorOmega HMC std e±Page, Tessa MCalculated using seacarb
19Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
20Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
21Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
22Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
23Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
24Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
25Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
26Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
108 data points

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