Citation:

PANGAEA.955746

Name: Seawater carbonate chemistry in the experiment of transcriptomic responses of coralline algae to global change stressors
Published: 2022
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Gene expression (incl. proteomics); Laboratory experiment; Macroalgae; Plantae; Porolithon onkodes; Rhodophyta; Single species; South Pacific; Sporolithon durum; Temperature; Tropical

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Abstract:

Crustose coralline algae (CCA) are calcifying red macroalgae that play important ecological roles including stabilisation of reef frameworks and provision of settlement cues for a range of marine invertebrates. Previous research into the responses of CCA to ocean warming (OW) and ocean acidification (OA) have found magnitude of effect to be species-specific. Response to OW and OA could be linked to divergent underlying molecular processes across species. Here we show Sporolithon durum, a species that exhibits low sensitivity to climate stressors, had little change in metabolic performance and did not significantly alter the expression of any genes when exposed to temperature and pH perturbations. In contrast, Porolithon onkodes, a major coral reef builder, reduced photosynthetic rates and had a labile transcriptomic response with over 400 significantly differentially expressed genes, with differential regulation of genes relating to physiological processes such as carbon acquisition and metabolism. The differential gene expression detected in P. onkodes implicates possible key metabolic pathways, including the pentose phosphate pathway, in the stress response of this species. We suggest S. durum is more resistant to OW and OA than P. onkodes, which demonstrated a high sensitivity to climate stressors and may have limited ability for acclimatisation. Understanding changes in gene expression in relation to physiological processes of CCA could help us understand and predict how different species will respond to, and persist in, future ocean conditions predicted for 2100.

Keyword(s):

Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Gene expression (incl. proteomics); Laboratory experiment; Macroalgae; Plantae; Porolithon onkodes; Rhodophyta; Single species; South Pacific; Sporolithon durum; Temperature; Tropical

Related to:

Page, Tessa M; McDougall, Carmel; Bar, Ido; Diaz-Pulido, Guillermo (2022): Transcriptomic stability or lability explains sensitivity to climate stressors in coralline algae. BMC Genomics, 23(1), 729, https://doi.org/10.1186/s12864-022-08931-9

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Page, Tessa M; McDougall, Carmel (2022): Transcriptomic responses of coralline algae to global change stressors. Open Science Framework, https://doi.org/10.17605/OSF.IO/2NKR4

Page, Tessa M; McDougall, Carmel; Bar, Ido; Diaz-Pulido, Guillermo (2022): Transcriptomic responses of coralline algae. Gene Expression Omnibus of the National Center for Biotechnology Information, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE211882

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-02-16.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Page, Tessa M		Study
2	Treatment	Treat		Page, Tessa M
3	Salinity	Sal		Page, Tessa M
4	Salinity, standard error	Sal std e	±	Page, Tessa M
5	Temperature, water	Temp	°C	Page, Tessa M
6	Temperature, water, standard error	T std e	±	Page, Tessa M
7	pH	pH		Page, Tessa M	Potentiometric	total scale
8	pH, standard error	pH std e	±	Page, Tessa M	Potentiometric	total scale
9	Alkalinity, total	AT	µmol/kg	Page, Tessa M	Potentiometric titration
10	Alkalinity, total, standard error	AT std e	±	Page, Tessa M	Potentiometric titration
11	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Page, Tessa M	Calculated using seacarb
12	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Page, Tessa M	Calculated using seacarb
13	Bicarbonate ion	[HCO3]-	µmol/kg	Page, Tessa M	Calculated using seacarb
14	Bicarbonate ion, standard error	[HCO3]- std e	±	Page, Tessa M	Calculated using seacarb
15	Carbonate ion	[CO3]2-	µmol/kg	Page, Tessa M	Calculated using seacarb
16	Carbonate ion, standard error	[CO3]2- std e	±	Page, Tessa M	Calculated using seacarb
17	High magnesium calcite saturation state	Omega HMC		Page, Tessa M	Calculated using seacarb
18	High magnesium calcite saturation state, standard error	Omega HMC std e	±	Page, Tessa M	Calculated using seacarb
19	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
20	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
21	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
22	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
23	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
24	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
25	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
26	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
27	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

108 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (Study)	2 Treat	3 Sal	4 Sal std e [±]	5 Temp [°C]	6 T std e [±]	7 pH (total scale, Potentiometric)	8 pH std e [±] (total scale, Potentiometric)	9 AT [µmol/kg] (Potentiometric titration)	10 AT std e [±] (Potentiometric titration)	11 pCO2water_SST_wet [µatm] (Calculated using seacarb)	12 pCO2water_SST_wet std e [±] (Calculated using seacarb)	13 [HCO3]- [µmol/kg] (Calculated using seacarb)	14 [HCO3]- std e [±] (Calculated using seacarb)	15 [CO3]2- [µmol/kg] (Calculated using seacarb)	16 [CO3]2- std e [±] (Calculated using seacarb)	17 Omega HMC (Calculated using seacarb)	18 Omega HMC std e [±] (Calculated using seacarb)	19 CSC flag (Calculated using seacarb afte...)	20 CO2 [µmol/kg] (Calculated using seacarb afte...)	21 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	22 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	23 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	24 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	25 DIC [µmol/kg] (Calculated using seacarb afte...)	26 Omega Arg (Calculated using seacarb afte...)	27 Omega Cal (Calculated using seacarb afte...)
laboratory	27.2 °C + pH 8.00	35.5	0.2	27.12	0.060	8.00	0.005	2291.49	0.653	454.190	8.178	1777.70	4.33	209.14	1.78	1.088	0.01	8	11.95	444.51	445.90	1775.14	209.83	1996.91	3.35	5.05
laboratory	29.5 °C + pH 8.00	35.5	0.2	29.29	0.071	7.99	0.003	2290.71	0.875	477.260	3.982	1768.71	2.98	212.72	1.34	1.135	0.03	8	11.60	454.46	455.84	1752.35	218.95	1982.90	3.54	5.29
laboratory	27.2 °C + pH 7.7	35.5	0.2	27.18	0.051	7.70	0.002	2290.91	0.811	1020.440	7.297	2005.62	1.86	116.54	7.98	0.606	0.02	8	26.82	999.43	1002.55	1999.64	118.72	2145.18	1.90	2.86
laboratory	29.5 °C + pH 7.7	35.5	0.2	29.48	0.071	7.69	0.003	2290.31	0.722	1028.482	7.076	1984.58	1.54	125.02	6.85	0.672	0.03	8	26.11	1027.64	1030.75	1983.91	125.09	2135.11	2.02	3.02