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Ruff, S Emil; Humez, Pauline; Hrabe de Angelis, Isabella; Diao, Muhe; Nightingale, Michael; Cho, Sara; Connors, Liam; Kuloyo, Olukayode O; Seltzer, Alan; Bowman, Samuel; Wankel, Scott D; McClain, Cynthia N; Mayer, Bernhard; Strous, Marc (2023): Contextual and environmental data characterizing 138 samples from Canadian groundwater ecosystems [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.953760, In: Ruff, SE et al. (2022): Hydrogen and dark oxygen drive microbial productivity in diverse groundwater ecosystems [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.952473

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Abstract:
Groundwater ecosystems are globally widespread yet still poorly understood. We therefore investigated contextual and environmental data characterizing 138 groundwater samples from Canadian groundwater ecosystems. The chemical, physical, gas, isotopic, and microbiological measurements were taken from aquifers in the Canadian Prairie between 2015 and 2020. The study area comprised 14 major aquifers and a geographic area of ~210.000 km2. The goal of the study was to understand the links between the biogeochemistry and microbial ecology of groundwater ecosystems in diverse geological settings on a broad spatial scale. Details concerning methods, results and conclusions can be found in the associated publication by Ruff et al. 2023.
Keyword(s):
aquifer; biogeochemistry; communities; groundwater; microbes
Coverage:
Median Latitude: 52.147748 * Median Longitude: -113.098970 * South-bound Latitude: 49.027319 * West-bound Longitude: -119.397742 * North-bound Latitude: 58.995364 * East-bound Longitude: -110.006390
Date/Time Start: 2016-01-19T00:00:00 * Date/Time End: 2017-12-12T00:00:00
Minimum ELEVATION: 334.0 m a.s.l. * Maximum ELEVATION: 1315.3 m a.s.l.
Event(s):
GOWN_Airdrie * Latitude: 51.234343 * Longitude: -114.000246 * Date/Time: 2017-06-06T00:00:00 * Location: Canadian Prairie * Method/Device: Field measurement
GOWN_Barons * Latitude: 49.992950 * Longitude: -113.076617 * Date/Time: 2016-10-25T00:00:00 * Location: Canadian Prairie * Method/Device: Field measurement
GOWN_Barrhead * Latitude: 54.035906 * Longitude: -114.397767 * Date/Time: 2016-08-17T00:00:00 * Location: Canadian Prairie * Method/Device: Field measurement
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventRuff, S Emil
2DATE/TIMEDate/TimeRuff, S EmilGeocode – Date of groundwater sampling
3LATITUDELatitudeRuff, S EmilGeocode
4LONGITUDELongitudeRuff, S EmilGeocode
5ELEVATIONElevationm a.s.l.Ruff, S EmilGeocode – Elevation on top of well casing
6Accession number, geneticsAccess no genRuff, S EmilNCBI BioProject accession number
7Accession number, geneticsAccess no genRuff, S EmilNCBI BioSample accession number
8Sequence identifierSeqIDRuff, S EmilName of the bacterial 16S rRNA gene amplicon sequence dataset; variable gene region v3-v4
9Sequence identifierSeqIDRuff, S EmilName of the archaeal 16S rRNA gene amplicon sequence dataset; variable gene region v6-v9
10WellWellRuff, S EmilWell name of Groundwater Observation Well Network (GOWN)
11Well, identifierWell IDRuff, S EmilWell number of Groundwater Observation Well Network (GOWN)
12Sample code/labelSample labelRuff, S EmilUnique Groundwater Observation Well Network (GOWN) identifier
13Sample code/label 2Sample label 2Ruff, S EmilNCBI BioSample name
14Sample volumeSamp volmlRuff, S EmilVolume of water used for centrifugation (and later DNA extraction)
15Cell pellet, sizeCell pellet sizeRuff, S EmilSize of the cell/sediment pellet after centrifugation
16Cell pellet, colorCell pellet colorRuff, S Emil
17Cell pellet, consistencyCell pellet consistencyRuff, S Emil
18DNA concentrationDNA concng/µlRuff, S Emil
19CellCell#/mlRuff, S EmilCells per ml groundwater
20Sample IDSample IDRuff, S EmilSample ID used in Figure 4 of the study Ruff et al. 2023
21Cells, totalCells total#Ruff, S EmilTotal number of cells counted per sample
22Number of squaresN square#Ruff, S Emilcounted per Grid. The microscopy counting grid has 10x10 = 100 squares
23Cell counts, standard errorCell counts standard error±Ruff, S Emil
24Cell counts, standard deviationCell count std dev±Ruff, S Emil
25Storage durationStorage durationdaysRuff, S Emilat 4°C before sample processing
26Methane, dissolvedCH4-Cµg/lRuff, S Emil
27EthaneC2H6µg/lRuff, S Emil
28PropaneC3H8µg/lRuff, S Emil
29Carbon dioxideCO2mg/lRuff, S Emil
30OxygenO2mg/lRuff, S Emilmeasured in the laboratory
31OxygenO2mg/lRuff, S Emilmeasured in the field
32Nitrogen, totalTNmg/lRuff, S Emil
33Gas drynessGas dryRuff, S EmilFG; C1/C2 ratio; Dryness of methane and ethane
34Gas drynessGas dryRuff, S EmilFG; C1/Ctotal; Dryness of all gaseous hydrocarbons
35δ13C, methaneδ13C CH4‰ PDBRuff, S EmilC Isotopic signature of methane free gas (FG)
36δ13C, carbon dioxideδ13C CO2‰ PDBRuff, S EmilC Isotopic signature of carbon dioxide free gas (FG)
37δ Deuterium, methaneδD CH4‰ SMOWRuff, S EmilD Isotopic signature of methane free gas (FG)
38δ13C, methaneδ13C CH4‰ PDBRuff, S EmilC Isotopic signature of methane dissolved gas (DG)
39δ Deuterium, methaneδD CH4‰ SMOWRuff, S EmilD Isotopic signature of methane dissolved gas (DG)
40δ13C, carbon dioxideδ13C CO2‰ PDBRuff, S EmilC Isotopic signature of carbon dioxide dissolved gas (DG)
41δ18O, waterδ18O H2O‰ SMOWRuff, S EmilO isotopic signature of water
42δ Deuterium, waterδD H2O‰ SMOWRuff, S EmilD isotopic signature of water
43δ34S, sulfateδ34S [SO4]2-‰ CDTRuff, S EmilS isotopic signature of sulfate
44δ18O, sulfateδ18O [SO4]2-‰ SMOWRuff, S EmilO isotopic signature of sulfate
45δ13C, dissolved inorganic carbonδ13C DIC‰ PDBRuff, S EmilC isotopic signature of dissolved inorganic carbon
46δ15N, nitrateδ15N NO3‰ airRuff, S EmilN isotopic signature of nitrate
47δ18O, nitrateδ18O NO3Ruff, S EmilO isotopic signature of nitrate
48Alkalinity, totalATmg/lRuff, S EmilCalcium carbonate alkalinity
49Bicarbonate ion[HCO3]-mg/lRuff, S EmilCalculated
50Calcium, dissolvedCa dissmg/lRuff, S Emil
51ChlorideCl-mg/lRuff, S Emil
52FluorideF-mg/lRuff, S Emil
53Hardness of waterHardness of watermg/lRuff, S EmilCalcium carbonate hardness
54Iron 2+Fe2+mg/lRuff, S EmilDissolved iron
55MagnesiumMg2+mg/lRuff, S Emil
56ManganeseMnmg/lRuff, S Emil
57AmmoniaNH3mg/lRuff, S Emil
58Nitrate-nitrogenNO3-Nmg/lRuff, S Emil
59Nitrite-nitrogenNO2-Nmg/lRuff, S Emil
60Nitrogen, totalTNmg/lRuff, S EmilCalculated
61pHpHRuff, S EmilMeasured in the field
62pHpHRuff, S EmilMeasured in the laboratory
63Phosphate[PO4]3-mg/lRuff, S Emil
64PotassiumK+mg/lRuff, S Emil
65Silica, reactive solubleSRSiµg/lRuff, S Emil
66SodiumNa+mg/lRuff, S Emil
67Conductivity, specificCondµS/cmRuff, S EmilMeasured in the field
68Conductivity, specificCondµS/cmRuff, S EmilMeasured in the laboratory
69Sulfate[SO4]2-mg/lRuff, S Emil
70Total dissolved solidsTDSmg/lRuff, S EmilCalculated
71Hydrochemical facies, main saltHydrochem facies main saltRuff, S Emilmain salt defining hydrochemical facies
72Age, categoryAge catRuff, S Emilbased on hydrochemical facies
73Age, datedAge datedka BPRuff, S EmilAge, 14C AMSradiocarbon age; BP; detection limit ~42,000 years, samples dated much older were changed to 44444 years []
74Age, dated, uncertaintyAge dated unc±Ruff, S EmilAge, 14C AMSradiocarbon age; BP
75Fraction modern carbonF14CRuff, S EmilAge, 14C AMS, Mini Carbon Dating System (MICADAS)detection limit ~0.0056, all samples with lower F were changed to 0
76Fraction modern carbon, errorF14C e±Ruff, S EmilAge, 14C AMS, Mini Carbon Dating System (MICADAS)
77Carbon-14, modernpMC%Ruff, S Emilfraction modern
78Carbon-14, modern, errorpMC e±Ruff, S Emil
79Age, groundwaterAge GWaRuff, S Emil3H Tritium age datingdetection limit~0.8, samples below that value were changed to 0
80Age, groundwater, uncertaintyAge GW unc±Ruff, S Emil3H Tritium age dating
81Sampling dateSampling dateRuff, S EmilDate of first sampling of well
82BasinBasinRuff, S EmilDrainage basin
83Sub-basinSub-basinRuff, S EmilDrainage sub basin
84Sampling dateSampling dateRuff, S EmilDrill date
85Well, diameterWell diammmRuff, S Emil
86Well, depthWell depthmRuff, S Emil
87Well, materialWell matRuff, S EmilWell casing materials
88Depth, samplingDepth samplingmRuff, S EmilBladder pump intake depth
89Depth, wellDepth wellmRuff, S EmilDepth to top of screen from top of well casing
90Depth, wellDepth wellmRuff, S EmilDepth to bottom of screen from top of well casing
91Depth, samplingDepth samplingmRuff, S EmilBladder pump intake relative to screen top
92Well, typeWell typeRuff, S Emil
93Lithology/composition/faciesLithologyRuff, S Emil
94Aquifer typeAquiferRuff, S Emil
95FormationFormationRuff, S EmilGeologic formation type
96Depth, descriptionDepth descRuff, S EmilWell depth category
97Aquifer, locationAquifer locRuff, S Emil
98Sampling dateSampling dateRuff, S EmilDate of first sampling for water quality monitoring
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
9856 data points

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