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Ruff, S Emil; Humez, Pauline; Hrabe de Angelis, Isabella; Diao, Muhe; Nightingale, Michael; Cho, Sara; Connors, Liam; Kuloyo, Olukayode O; Seltzer, Alan; Bowman, Samuel; Wankel, Scott D; McClain, Cynthia N; Mayer, Bernhard; Strous, Marc (2022): Hydrogen and dark oxygen drive microbial productivity in diverse groundwater ecosystems [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.952473

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Abstract:
Around 50% of humankind relies on groundwater aquifers as a source of drinking water. We investigated the age, geochemistry, and microbiology of 138 groundwaters from 87 monitoring wells (<250m depth) located in 14 aquifers in the Canadian Prairie. This data collection contains geological, physical, geochemical, gas, isotope and other contextual data, as well as microbial cell counts, 16S rRNA gene sequences, community diversity metrics and micrographs. Geochemistry and microbial ecology showed consistent trends suggesting large-scale aerobic and anaerobic hydrogen, methane, nitrogen, and sulfur cycling carried out by diverse microbial communities. Older groundwaters, especially of organic carbon-rich strata, contained on average more cells (up to 1.4'107/mL) than younger groundwaters, challenging current estimates of global groundwater population sizes. Substantial concentrations of dissolved oxygen (0.52±0.12mg/L [mean±SE]; n=57) in older groundwaters could support aerobic lifestyles in subsurface ecosystems at an unprecedented scale. Metagenomics, oxygen isotope analyses and mixing models indicated that “dark oxygen” was produced in situ via microbial dismutation. We show that ancient groundwaters sustain productive communities and highlight an overlooked oxygen source in present and past subsurface ecosystems of Earth.
Keyword(s):
aquifer; biogeochemistry; communities; Field measurements; groundwater; microbes
Coverage:
Median Latitude: 53.360719 * Median Longitude: -114.105564 * South-bound Latitude: 49.027319 * West-bound Longitude: -119.397742 * North-bound Latitude: 58.995364 * East-bound Longitude: -110.006390
Date/Time Start: 2016-01-19T00:00:00 * Date/Time End: 2017-12-12T00:00:00
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
10 datasets

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Datasets listed in this bundled publication

  1. Ruff, SE; Humez, P; Hrabe de Angelis, I et al. (2022): Relative abundances of archaeal amplicon sequence variants (ASV) from Canadian Prairie groundwater ecosystems. https://doi.org/10.1594/PANGAEA.952298
  2. Ruff, SE; Humez, P; Hrabe de Angelis, I et al. (2022): Archaeal diversity of Canadian Prairie groundwater ecosystems. https://doi.org/10.1594/PANGAEA.952362
  3. Ruff, SE; Humez, P; Hrabe de Angelis, I et al. (2022): Nucleotide sequences of archaeal amplicon sequence variants (ASV) from Canadian Prairie groundwater ecosystems. https://doi.org/10.1594/PANGAEA.952291
  4. Ruff, SE; Humez, P; Hrabe de Angelis, I et al. (2022): Nucleotide sequences of bacterial amplicon sequence variants (ASV) from Canadian Prairie groundwater ecosystems. https://doi.org/10.1594/PANGAEA.952292
  5. Ruff, SE; Humez, P; Hrabe de Angelis, I et al. (2022): Relative abundances of bacterial amplicon sequence variants (ASV) from Canadian Pairie groundwater ecosystems. https://doi.org/10.1594/PANGAEA.952419
  6. Ruff, SE; Humez, P; Hrabe de Angelis, I et al. (2022): Microbial cell counts from Canadian Prairie groundwater ecosystems. https://doi.org/10.1594/PANGAEA.952331
  7. Ruff, SE; Humez, P; Hrabe de Angelis, I et al. (2023): Contextual and environmental data characterizing 138 samples from Canadian groundwater ecosystems. https://doi.org/10.1594/PANGAEA.953760
  8. Ruff, SE; Humez, P; Hrabe de Angelis, I et al. (2022): Bacterial diversity of Canadian Prairie groundwater ecosystems. https://doi.org/10.1594/PANGAEA.952402
  9. Ruff, SE; Humez, P; Hrabe de Angelis, I et al. (2022): Microscopic images of 45 groundwater samples from Canadian Prairie groundwater ecosystems. https://doi.org/10.1594/PANGAEA.952296
  10. Ruff, SE; Humez, P; Hrabe de Angelis, I et al. (2022): Microbial oxygen consumption from Canadian Prairie groundwater ecosystems. https://doi.org/10.1594/PANGAEA.952335