Citation:

PANGAEA.953109

Name: Seawater carbonate chemistry and proton gradients across the coral calcifying cell layer
Published: 2022-12-23
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Acid-base regulation; Animalia; Benthic animals; Benthos; Cnidaria; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Laboratory strains; Not applicable; Other; Single species; Stylophora pistillata

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Abstract:

In corals, pH regulation of the extracellular calcifying medium (ECM) by the calcifying cell layer is a crucial step in the calcification process and is potentially important to influencing how corals respond to ocean acidification. Here, we analyzed the growing edge of the reef coral Stylophora pistillata to make the first characterization of the proton gradient across the coral calcifying epithelium. At seawater pH 8 we found that while the calcifying epithelium elevates pH in the ECM on its apical side above that of seawater, pH on its basal side in the mesoglea is markedly lower, highlighting that the calcifying cells are exposed to a microenvironment distinct from the external environment. Coral symbiont photosynthesis elevates pH in the mesoglea, but experimental ocean acidification and decreased seawater inorganic carbon concentration lead to large declines in mesoglea pH relative to the ECM, which is maintained relatively stable. Together, our results indicate that the coral calcifying epithelium is functionally polarized and that environmental variation impacts pHECM regulation through its effects on the basal side of the calcifying cells.

Keyword(s):

Acid-base regulation; Animalia; Benthic animals; Benthos; Cnidaria; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Laboratory strains; Not applicable; Other; Single species; Stylophora pistillata

Supplement to:

Venn, Alexander A; Tambutté, Eric; Comeau, Steeve; Tambutté, Sylvie (2022): Proton gradients across the coral calcifying cell layer: Effects of light, ocean acidification and carbonate chemistry. Frontiers in Marine Science, 9, 973908, https://doi.org/10.3389/fmars.2022.973908

Original version:

Venn, Alexander A; Tambutté, Eric; Steeve, Comeau; Tambutté, Sylvie (2022): Proton gradients across the coral calcifying cell layer: effects of light, ocean acidification and carbonate chemistry. PANGAEA, https://doi.org/10.1594/PANGAEA.949400

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-12-23.

The experiments were done in the lab on coral samples that have been held in culture in Monaco for the last 30 years. The data file contains pH data from 3 experiments. In experiment 1corals were kept at seawater pH 8. In experiment 2. Corals were exposed to seawater acidification. In experiment 3 corals were exposed to manipulated DIC concentrations. The data are pH measurements taken from 3 internal compartments of the coral Stylophora pistillata: the mesoglea, the calcifying cells and the ECM.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Venn, Alexander A		Study
2	Species, unique identification	Species UID		Venn, Alexander A
3	Species, unique identification (URI)	Species UID (URI)		Venn, Alexander A
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Venn, Alexander A
5	Experiment	Exp		Venn, Alexander A
6	Experiment/study setup	Setup		Venn, Alexander A
7	Comment	Comment		Venn, Alexander A		Figure
8	Treatment	Treat		Venn, Alexander A
9	Temperature, water	Temp	°C	Venn, Alexander A	Thermometer
10	Salinity	Sal		Venn, Alexander A
11	pH	pH		Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
12	pH, standard deviation	pH std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
13	pH	pH		Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
14	pH, standard deviation	pH std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
15	pH, extracellular	pHe		Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
16	pH, extracellular, standard deviation	pHe std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
17	pH, extracellular	pHe		Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
18	pH, extracellular, standard deviation	pHe std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
19	pH, intracellular	pH in		Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
20	pH, intracellular, standard deviation	pH in std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
21	pH, intracellular	pH in		Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
22	pH, intracellular, standard deviation	pH in std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
23	pH, mesoglea	ph mg		Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
24	pH, mesoglea	ph mg		Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
25	pH, mesoglea	ph mg		Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
26	pH, mesoglea	ph mg		Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
27	Alkalinity, total	AT	µmol/kg	Venn, Alexander A
28	pH	pH		Venn, Alexander A		total scale
29	Carbon, inorganic, dissolved	DIC	µmol/kg	Venn, Alexander A
30	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Venn, Alexander A
31	Bicarbonate ion	[HCO3]-	µmol/kg	Venn, Alexander A
32	Carbonate ion	[CO3]2-	µmol/kg	Venn, Alexander A
33	Aragonite saturation state	Omega Arg		Venn, Alexander A
34	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

350 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (Study)	2 Species UID	3 Species UID (URI)	4 Species UID (Semantic URI)	5 Exp	6 Setup	7 Comment (Figure)	8 Treat	9 Temp [°C] (Thermometer)	10 Sal	11 pH (Light, Confocal Microscope, L...)	12 pH std dev [±] (Light, Confocal Microscope, L...)	13 pH (Dark, Confocal Microscope, Le...)	14 pH std dev [±] (Dark, Confocal Microscope, Le...)	15 pHe (Light, Confocal Microscope, L...)	16 pHe std dev [±] (Light, Confocal Microscope, L...)	17 pHe (Dark, Confocal Microscope, Le...)	18 pHe std dev [±] (Dark, Confocal Microscope, Le...)	19 pH in (Light, Confocal Microscope, L...)	20 pH in std dev [±] (Light, Confocal Microscope, L...)	21 pH in (Dark, Confocal Microscope, Le...)	22 pH in std dev [±] (Dark, Confocal Microscope, Le...)	23 ph mg (Light, Confocal Microscope, L...)	24 ph mg (Light, Confocal Microscope, L...)	25 ph mg (Dark, Confocal Microscope, Le...)	26 ph mg (Dark, Confocal Microscope, Le...)	27 AT [µmol/kg]	28 pH (total scale)	29 DIC [µmol/kg]	30 pCO2water_SST_wet [µatm]	31 [HCO3]- [µmol/kg]	32 [CO3]2- [µmol/kg]	33 Omega Arg	34 CSC flag (Calculated using seacarb afte...)	35 CO2 [µmol/kg] (Calculated using seacarb afte...)	36 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	37 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	38 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	39 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	40 DIC [µmol/kg] (Calculated using seacarb afte...)	41 Omega Arg (Calculated using seacarb afte...)	42 Omega Cal (Calculated using seacarb afte...)
laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	pH measurements	laboratory	1D	Seawater pH 8	25	37	8.00		8.00		8.28	0.03	8.19	0.03	7.46	0.04	7.43	0.02	7.79	0.15	7.60	0.08	2436.14	8.03	2102.87	415.32	1854.56	236.74	3.68	8	12.15	432.31	433.69	1878.61	227.69	2118.45	3.56	5.39
laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	pH measurements	laboratory	2	Seawater pH 7.2	25	37	7.23	0.09	7.27	0.06	7.81	0.10	7.82	0.08	7.30	0.03	7.25	0.07	7.09	0.16	7.02	0.16	2463.03	7.24	2485.18	3275.84	2345.84	48.07	0.75	8	94.91	3377.96	3388.82	2351.87	45.73	2492.51	0.72	1.08
laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	pH measurements	laboratory	2	Seawater pH 7.4	25	37	7.48	0.02	7.44	0.02	7.85	0.09	7.82	0.03	7.39	0.00	7.34	0.06	7.30	0.14	7.25	0.13	2458.86	7.44	2408.58	2013.85	2278.63	73.83	1.15	8	58.24	2072.89	2079.54	2287.29	70.48	2416.02	1.10	1.67
laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	pH measurements	laboratory	2	Seawater pH 7.8	25	37	7.81	0.05	7.86	0.07	8.04	0.05	7.95	0.07	7.47	0.03	7.44	0.03	7.49	0.13	7.44	0.06	2437.78	7.81	2231.86	771.02	2053.60	156.78	2.44	8	22.23	791.02	793.56	2068.42	151.17	2241.82	2.37	3.58
laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	pH measurements	laboratory	2	Seawater pH 8.0	25	37	8.00	0.06	8.05	0.07	8.28	0.04	8.19	0.05	7.47	0.04	7.45	0.03	7.79	0.13	7.60	0.10	2436.14	8.03	2102.87	415.32	1854.56	236.74	3.68	8	12.15	432.31	433.69	1878.61	227.69	2118.45	3.56	5.39
laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	pH measurements	laboratory	3	High [DIC]	25	37	7.95	0.07	7.96	0.08	8.23	0.05	8.11	0.02	7.46	0.05	7.45	0.07	7.90	0.01	7.76	0.25	3278.00	7.91	2968.50		2696.00	248.50	3.89	8	23.33	830.34	833.00	2705.49	245.90	2974.72	3.85	5.82
laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	pH measurements	laboratory	3	Ambient [DIC]	25	37	7.91	0.04	7.92	0.01	8.15	0.03	8.02	0.05	7.37	0.03	7.40	0.05	7.70	0.13	7.51	0.11	2460.50	7.89	2219.00		2022.50	178.50	2.79	8	18.09	643.66	645.72	2026.28	177.91	2222.28	2.78	4.21
laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	pH measurements	laboratory	3	Low [DIC]	25	37	7.93	0.04	7.94	0.02	7.97	0.09	7.93	0.09					7.24	0.07	6.93	0.06	1774.00	7.94	1553.00		1402.00	141.00	2.21	8	11.22	399.15	400.43	1409.89	138.90	1560.01	2.17	3.29
laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	pH measurements	laboratory	3	Very Low [DIC]	25	37	7.92	0.03	7.92	0.07	7.92	0.02	7.91	0.05	7.24	0.01	7.25	0.04	7.00	0.19	6.85	0.06	1001.00	7.93	846.00		766.50	73.50	1.15	8	6.35	225.83	226.56	770.48	73.33	850.15	1.15	1.74