Citation:

PANGAEA.949400

Name: Proton gradients across the coral calcifying cell layer: effects of light, ocean acidification and carbonate chemistry
Published: 2022-10-06
License: https://creativecommons.org/licenses/by/4.0/
Keywords: biomineralization; Climate change; Laboratory experiment; pH regulation; physiology; scleractinians

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Published: 2022-10-06 • DOI registered: 2022-10-21

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Abstract:

Internal pH measurements made in the extracellular calcifying medium (ECM), calcifying (calicoblastic) epithelium and mesoglea of the coral Stylophora pistillata using the fluorescent dye SNARF-1 and confocal microscopy. The measurements were made in light and darkness three experiments. Experiment 1 involved using coral samples maintained at pH 8 seawater. Experiment 2 involved placing samples in 4 seawater acidification conditions: pH 8, 7.8, 7.4 and 7.2 for 1 week. Experiment 3 involved placing samples in 4 levels of dissolved inorganic carbon concentration: elevated; ambient, low and very low for one week. The research was carried out at the Centre Scientifique de Monaco between 2014-2017. The aim of the experiment was to determine the pH gradient across the calcifying cell layer and determine how it responded to the three experiments.

Keyword(s):

biomineralization; Climate change; Laboratory experiment; pH regulation; physiology; scleractinians

Related to:

Venn, Alexander A; Tambutté, Eric; Comeau, Steeve; Tambutté, Sylvie (2022): Proton gradients across the coral calcifying cell layer: Effects of light, ocean acidification and carbonate chemistry. Frontiers in Marine Science, 9, 973908, https://doi.org/10.3389/fmars.2022.973908

Event(s):

Laboratory-experiments * Method/Device: Experiment (EXP)

Comment:

The experiments were done in the lab on coral samples that have been held in culture in Monaco for the last 30 years. The data file contains pH data from 3 experiments. In experiment 1corals were kept at seawater pH 8. In experiment 2. Corals were exposed to seawater acidification. In experiment 3 corals were exposed to manipulated DIC concentrations. The data are pH measurements taken from 3 internal compartments of the coral Stylophora pistillata: the mesoglea, the calcifying cells and the ECM.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Experiment	Exp		Venn, Alexander A
2	Experiment/study setup	Setup		Venn, Alexander A
3	Species, unique identification	Species UID		Venn, Alexander A
4	Species, unique identification (URI)	Species UID (URI)		Venn, Alexander A
5	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Venn, Alexander A
6	Comment	Comment		Venn, Alexander A		Figure
7	Treatment	Treat		Venn, Alexander A
8	Temperature, water	Temp	°C	Venn, Alexander A	Thermometer
9	Salinity	Sal		Venn, Alexander A
10	pH	pH		Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
11	pH, standard deviation	pH std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
12	pH	pH		Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
13	pH, standard deviation	pH std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
14	pH, extracellular	pHe		Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
15	pH, extracellular, standard deviation	pHe std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
16	pH, extracellular	pHe		Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
17	pH, extracellular, standard deviation	pHe std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
18	pH, intracellular	pH in		Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
19	pH, intracellular, standard deviation	pH in std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
20	pH, intracellular	pH in		Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
21	pH, intracellular, standard deviation	pH in std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
22	pH, mesoglea	ph mg		Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
23	pH, mesoglea, standard deviation	pH mg std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
24	pH, mesoglea	ph mg		Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
25	pH, mesoglea, standard deviation	pH mg std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

201 data points

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