Citation:

PANGAEA.949400

Name: Proton gradients across the coral calcifying cell layer: effects of light, ocean acidification and carbonate chemistry
Published: 2022-10-06
License: https://creativecommons.org/licenses/by/4.0/
Keywords: biomineralization; Climate change; Laboratory experiment; pH regulation; physiology; scleractinians

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Abstract:

Internal pH measurements made in the extracellular calcifying medium (ECM), calcifying (calicoblastic) epithelium and mesoglea of the coral Stylophora pistillata using the fluorescent dye SNARF-1 and confocal microscopy. The measurements were made in light and darkness three experiments. Experiment 1 involved using coral samples maintained at pH 8 seawater. Experiment 2 involved placing samples in 4 seawater acidification conditions: pH 8, 7.8, 7.4 and 7.2 for 1 week. Experiment 3 involved placing samples in 4 levels of dissolved inorganic carbon concentration: elevated; ambient, low and very low for one week. The research was carried out at the Centre Scientifique de Monaco between 2014-2017. The aim of the experiment was to determine the pH gradient across the calcifying cell layer and determine how it responded to the three experiments.

Keyword(s):

biomineralization; Climate change; Laboratory experiment; pH regulation; physiology; scleractinians

Related to:

Venn, Alexander A; Tambutté, Eric; Comeau, Steeve; Tambutté, Sylvie (2022): Proton gradients across the coral calcifying cell layer: Effects of light, ocean acidification and carbonate chemistry. Frontiers in Marine Science, 9, 973908, https://doi.org/10.3389/fmars.2022.973908

Event(s):

Laboratory-experiments * Method/Device: Experiment (EXP)

Comment:

The experiments were done in the lab on coral samples that have been held in culture in Monaco for the last 30 years. The data file contains pH data from 3 experiments. In experiment 1corals were kept at seawater pH 8. In experiment 2. Corals were exposed to seawater acidification. In experiment 3 corals were exposed to manipulated DIC concentrations. The data are pH measurements taken from 3 internal compartments of the coral Stylophora pistillata: the mesoglea, the calcifying cells and the ECM.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Experiment	Exp		Venn, Alexander A
2	Experiment/study setup	Setup		Venn, Alexander A
3	Species, unique identification	Species UID		Venn, Alexander A
4	Species, unique identification (URI)	Species UID (URI)		Venn, Alexander A
5	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Venn, Alexander A
6	Comment	Comment		Venn, Alexander A		Figure
7	Treatment	Treat		Venn, Alexander A
8	Temperature, water	Temp	°C	Venn, Alexander A	Thermometer
9	Salinity	Sal		Venn, Alexander A
10	pH	pH		Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
11	pH, standard deviation	pH std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
12	pH	pH		Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
13	pH, standard deviation	pH std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
14	pH, extracellular	pHe		Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
15	pH, extracellular, standard deviation	pHe std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
16	pH, extracellular	pHe		Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
17	pH, extracellular, standard deviation	pHe std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
18	pH, intracellular	pH in		Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
19	pH, intracellular, standard deviation	pH in std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
20	pH, intracellular	pH in		Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
21	pH, intracellular, standard deviation	pH in std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
22	pH, mesoglea	ph mg		Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
23	pH, mesoglea, standard deviation	pH mg std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Light
24	pH, mesoglea	ph mg		Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark
25	pH, mesoglea, standard deviation	pH mg std dev	±	Venn, Alexander A	Confocal Microscope, Leica, SP5	Dark

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

201 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Exp	2 Setup	3 Species UID	4 Species UID (URI)	5 Species UID (Semantic URI)	6 Comment (Figure)	7 Treat	8 Temp [°C] (Thermometer)	9 Sal	10 pH (Light, Confocal Microscope, L...)	11 pH std dev [±] (Light, Confocal Microscope, L...)	12 pH (Dark, Confocal Microscope, Le...)	13 pH std dev [±] (Dark, Confocal Microscope, Le...)	14 pHe (Light, Confocal Microscope, L...)	15 pHe std dev [±] (Light, Confocal Microscope, L...)	16 pHe (Dark, Confocal Microscope, Le...)	17 pHe std dev [±] (Dark, Confocal Microscope, Le...)	18 pH in (Light, Confocal Microscope, L...)	19 pH in std dev [±] (Light, Confocal Microscope, L...)	20 pH in (Dark, Confocal Microscope, Le...)	21 pH in std dev [±] (Dark, Confocal Microscope, Le...)	22 ph mg (Light, Confocal Microscope, L...)	23 pH mg std dev [±] (Light, Confocal Microscope, L...)	24 ph mg (Dark, Confocal Microscope, Le...)	25 pH mg std dev [±] (Dark, Confocal Microscope, Le...)
pH measurements	laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	1D	Seawater pH 8	25	37	8.00		8.00		8.28	0.03	8.19	0.03	7.46	0.04	7.43	0.02	7.79	0.15	7.60	0.08
pH measurements	laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	2	Seawater pH 7.2	25	37	7.23	0.09	7.27	0.06	7.81	0.10	7.82	0.08	7.30	0.03	7.25	0.07	7.09	0.16	7.02	0.16
pH measurements	laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	2	Seawater pH 7.4	25	37	7.48	0.02	7.44	0.02	7.85	0.09	7.82	0.03	7.39	0.00	7.34	0.06	7.30	0.14	7.25	0.13
pH measurements	laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	2	Seawater pH 7.8	25	37	7.81	0.05	7.86	0.07	8.04	0.05	7.95	0.07	7.47	0.03	7.44	0.03	7.49	0.13	7.44	0.06
pH measurements	laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	2	Seawater pH 8.0	25	37	8.00	0.06	8.05	0.07	8.28	0.04	8.19	0.05	7.47	0.04	7.45	0.03	7.79	0.13	7.60	0.10
pH measurements	laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	3	High [DIC]	25	37	7.95	0.07	7.96	0.08	8.23	0.05	8.11	0.02	7.46	0.05	7.45	0.07	7.90	0.01	7.76	0.25
pH measurements	laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	3	Ambient [DIC]	25	37	7.91	0.04	7.92	0.01	8.15	0.03	8.02	0.05	7.37	0.03	7.40	0.05	7.70	0.13	7.51	0.11
pH measurements	laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	3	Low [DIC]	25	37	7.93	0.04	7.94	0.02	7.97	0.09	7.93	0.09					7.24	0.07	6.93	0.06
pH measurements	laboratory	Stylophora pistillata	marinespecies.org	urn:lsid:marinespecies.org:taxname:206982	3	Very Low [DIC]	25	37	7.92	0.03	7.92	0.07	7.92	0.02	7.91	0.05	7.24	0.01	7.25	0.04	7.00	0.19	6.85	0.06