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Ruff, S Emil; Humez, Pauline; Hrabe de Angelis, Isabella; Diao, Muhe; Nightingale, Michael; Cho, Sara; Connors, Liam; Kuloyo, Olukayode O; Seltzer, Alan; Bowman, Samuel; Wankel, Scott D; McClain, Cynthia N; Mayer, Bernhard; Strous, Marc (2022): Archaeal diversity of Canadian Prairie groundwater ecosystems [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.952362, In: Ruff, SE et al. (2022): Hydrogen and dark oxygen drive microbial productivity in diverse groundwater ecosystems [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.952473

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Abstract:
Groundwater ecosystems are globally widespread yet still poorly understood. We investigated the microbiology of groundwater samples from various monitoring wells (<250m depth) located in 14 aquifers in the Canadian Prairie. This dataset contains archaeal community diversity indices based on 16S rRNA gene amplicons retrieved from 52 groundwater samples of aquifers in the Canadian Prairie, collected between 2015 and 2020. The study area comprised 14 major aquifers and a geographic area of ~210.000 km2. The goal of the study was to understand the links between the biogeochemistry and microbial ecology of groundwater ecosystems in diverse geological settings on a broad spatial scale. Details concerning DNA extraction, sequencing and sequence analyses can be found in the associated publication by Ruff et al. 2023.
Keyword(s):
aquifer; biogeochemistry; communities; groundwater; microbes
Coverage:
Median Latitude: 52.181562 * Median Longitude: -112.965514 * South-bound Latitude: 49.378358 * West-bound Longitude: -117.656667 * North-bound Latitude: 58.995364 * East-bound Longitude: -110.418144
Date/Time Start: 2016-03-15T00:00:00 * Date/Time End: 2017-12-09T00:00:00
Event(s):
GOWN_Airdrie  * Latitude: 51.234343 * Longitude: -114.000246 * Date/Time: 2017-06-06T00:00:00 * Location: Canadian Prairie * Method/Device: Field measurement
GOWN_Barons  * Latitude: 49.992950 * Longitude: -113.076617 * Date/Time: 2016-10-25T00:00:00 * Location: Canadian Prairie * Method/Device: Field measurement
GOWN_Canmore  * Latitude: 51.107306 * Longitude: -115.366444 * Date/Time: 2017-08-21T00:00:00 * Location: Canadian Prairie * Method/Device: Field measurement
Comment:
Note: Absolute singe sequence OTUs (operational taxonomic units), also abbreviated as SSOabs are OTUs (based on 16S rRNA gene sequences) that occur only once in an entire dataset. In this case, these OTUs would only occur once in all 138 samples. These are generally rare organisms.
Relative SSO are OTUs that occur only once in 1 sample, but also occur at least once in another sample. These OTUs are conditionally rare. They are rare in one environment, but may become abundant when conditions change.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 Event labelEventRuff, S Emil
2 DATE/TIMEDate/TimeRuff, S EmilGeocode
3 LATITUDELatitudeRuff, S EmilGeocode
4 LONGITUDELongitudeRuff, S EmilGeocode
5 Accession number, geneticsAccess no genRuff, S EmilNCBI BioProject accession number
6 Accession number, geneticsAccess no genRuff, S EmilNCBI BioSample accession number
7 Sequence identifierSeqIDRuff, S EmilName of the archaeal 16S rRNA gene amplicon sequence dataset; variable gene region v6-v9
8 WellWellRuff, S EmilWell name of Groundwater Observation Well Network (GOWN)
9 Well, identifierWell IDRuff, S EmilWell number of Groundwater Observation Well Network (GOWN)
10 Sample code/labelSample labelRuff, S EmilUnique Groundwater Observation Well Network (GOWN) identifier
11 Sample code/label 2Sample label 2Ruff, S EmilNCBI BioSample name
12 ReadsReads#Ruff, S EmilTotal reads; unsubsampled
13 Amplicon sequence variant, richnessASV, richnessRuff, S Emilunsubsampled; observed ASV
14 Amplicon sequence variant, absolute single sequenceASV abs single seq#Ruff, S Emilunsubsampled
15 Amplicon sequence variant, absolute single sequenceASV abs single seq%Ruff, S Emilunsubsampled
16 Amplicon sequence variant, relative single sequenceASV rel single seq#Ruff, S Emilunsubsampled
17 Amplicon sequence variant, relative single sequenceASV rel single seq%Ruff, S Emilunsubsampled
18 ReadsReads#Ruff, S EmilTotal reads; subsampled
19 Amplicon sequence variant, richnessASV, richnessRuff, S Emilsubsampled; observed ASV
20 Chao 1 richnessChao1 richRuff, S Emilsubsampled; estimated ASV
21 Inverse Simpson index of diversityInv Simpson IRuff, S Emilsubsampled
22 Shannon Diversity IndexH'Ruff, S Emilsubsampled
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
792 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Event		2 
Date/Time		3 
Latitude		4 
Longitude		5 
Access no gen
(NCBI BioProject accession number)		6 
Access no gen
(NCBI BioSample accession number)		7 
SeqID
(Name of the archaeal 16S rRNA...)		8 
Well
(Well name of Groundwater Obse...)		9 
Well ID
(Well number of Groundwater Ob...)		10 
Sample label
(Unique Groundwater Observatio...)		11 
Sample label 2
(NCBI BioSample name)		12 
Reads [#]
(Total reads; unsubsampled)		13 
ASV, richness
(unsubsampled; observed ASV)		14 
ASV abs single seq [#]
(unsubsampled)		15 
ASV abs single seq [%]
(unsubsampled)		16 
ASV rel single seq [#]
(unsubsampled)		17 
ASV rel single seq [%]
(unsubsampled)		18 
Reads [#]
(Total reads; subsampled)		19 
ASV, richness
(subsampled; observed ASV)		20 
Chao1 rich
(subsampled; estimated ASV)		21 
Inv Simpson I
(subsampled)		22 
H'
(subsampled)
GOWN_Ross_Creek 2016-08-2549.988372-110.461225insdc:PRJNA861683insdc:SAMN29923129GW114_BE029_S85_Av6Ross_Creek11416GWC16039Ross_Creek_114_16GWC16039106653300.000.0203230315.22.2
GOWN_Barons 2016-10-2549.992950-113.076617insdc:PRJNA861683insdc:SAMN29923148GW117_BE052_S34_Av6Barons11716GWC16055Barons_117_16GWC1605521879411.100.02032949423.23.7
GOWN_Gem_66_7 2016-07-0951.008536-112.237939insdc:PRJNA861683insdc:SAMN29923133GW121_BE034_S69_Av6Gem_66_712116GWC16046Gem_66_7_121_16GWC1604620799511.100.02032959518.03.7
GOWN_Stettler_South 2017-09-2852.317519-112.802697insdc:PRJNA861683insdc:SAMN29923221GW135_BE125_S128_Av6Stettler_South13517GWC17088Stettler_South_135_17GWC17088333493000.000.0203220232.51.2
GOWN_Stettler_North 2017-09-2752.332228-112.763439insdc:PRJNA861683insdc:SAMN29923220GW137_BE124_S96_Av6Stettler_North13717GWC17086Stettler_North_137_17GWC1708621915100.000.0203251514.92.5
GOWN_Wainwright 2016-11-3052.725775-110.848489insdc:PRJNA861683insdc:SAMN29923157GW140_BE061_S75_Av6Wainwright14016GWC16086Wainwright_140_16GWC1608613217900.000.02032881.10.3
GOWN_Wainwright2017-10-1752.725775-110.848489insdc:PRJNA861683insdc:SAMN29923227GW140_BE131_S70_Av6Wainwright14017GWC17052Wainwright_140_17GWC1705294371500.000.0203212131.80.8
GOWN_Hardisty 2017-03-0852.675336-111.322319insdc:PRJNA861683insdc:SAMN29923194GW142_BE098_S86_Av6Hardisty14217GWE00077Hardisty_142_17GWE00077144482100.000.0203216182.01.1
GOWN_Ponoka 2017-06-2952.683411-113.595553insdc:PRJNA861683insdc:SAMN29923181GW144_BE085_S90_Av6Ponoka14417GWE00008Ponoka_144_17GWE0000823059344.300.02032939416.73.6
GOWN_Rollyview 2017-12-0753.223167-113.350333insdc:PRJNA861683insdc:SAMN29923185GW152_BE089_S143_Av6Rollyview15217GWE00066Rollyview_152_17GWE0006661261800.000.0203217183.51.5
GOWN_Devon_Botanical_Garden 2016-08-1852.410211-113.762236insdc:PRJNA861683insdc:SAMN29923126GW160_BE026_S115_Av6Devon_Botanical_Garden16016GWE00077Devon_Botanical_Garden_160_16GWE0007771742200.014.5203219193.71.6
GOWN_Warner 2017-02-0849.378358-112.203003insdc:PRJNA861683insdc:SAMN29923190GW215_BE094_S98_Av6Warner21517GWC17043Warner_215_17GWC1704313184900.000.02032881.00.1
GOWN_Okotoks_N 2017-06-2950.650485-113.976734insdc:PRJNA861683insdc:SAMN29923182GW217_BE086_S16_Av6Okotoks_N21717GWC17029Okotoks_N_217_17GWC17029614020883.800.0203218720515.24.0
GOWN_Kirkpatrick 2017-06-1351.952740-111.443108insdc:PRJNA861683insdc:SAMN29923166GW228_BE070_S31_Av6Kirkpatrick22817GWC17014Kirkpatrick_228_17GWC17014104641500.000.0203213152.21.2
GOWN_Dewberry 2016-07-2853.588114-110.534417insdc:PRJNA861683insdc:SAMN29923121GW239_BE019_S80_Av6Dewberry23916GWE00055Dewberry_239_16GWE0005587731000.000.02032991.50.6
GOWN_Dewberry2017-06-2753.588114-110.534417insdc:PRJNA861683insdc:SAMN29923178GW239_BE082_S121_Av6Dewberry23917GWE00058Dewberry_239_17GWE00058145761400.000.0203212121.50.7
GOWN_Metiskow 2016-11-2952.421297-110.607236insdc:PRJNA861683insdc:SAMN29923156GW265_BE060_S123_Av6Metiskow26516GWC16089Metiskow_265_16GWC160892274600.000.02032661.30.5
GOWN_Metiskow2016-11-2952.421500-110.606833insdc:PRJNA861683insdc:SAMN29923155GW267_BE059_S43_Av6Metiskow26716GWC16092Metiskow_267_16GWC16092813724983.200.0203221924147.84.5
GOWN_Metiskow2017-10-1852.421500-110.606833insdc:PRJNA861683insdc:SAMN29923230GW267_BE134_S68_Av6Metiskow26717GWC17049Metiskow_267_17GWC17049916032561.800.0203227531525.34.5
GOWN_Sounding_Creek 2017-10-2551.571986-110.474725insdc:PRJNA861683insdc:SAMN29923233GW275_BE137_S88_Av6Sounding_Creek27517GWC17109Sounding_Creek_275_17GWC1710923561000.000.0203210101.30.6
GOWN_Cavendish 2017-07-2750.797364-110.418144insdc:PRJNA861683insdc:SAMN29923189GW277_BE093_S65_Av6Cavendish27717GWC17035Cavendish_277_17GWC17035221910921.800.0203210911021.33.7
GOWN_Forty_Mile_Coulee 2017-06-0749.682889-111.424458insdc:PRJNA861683insdc:SAMN29923184GW286_BE088_S71_Av6Forty_Mile_Coulee28617GWC17027Forty_Mile_Coulee_286_17GWC1702722571200.000.0203212123.71.5
GOWN_Crestomere 2017-11-3052.745200-113.972397insdc:PRJNA861683insdc:SAMN29923239GW291_BE143_S144_Av6Crestomere29117GWC17123Crestomere_291_17GWC1712336921100.000.0203211113.41.5
GOWN_Leedale_Shallow 2017-11-1052.537473-114.576872insdc:PRJNA861683insdc:SAMN29923226GW3002_BE130_S32_Av6Leedale_Shallow300217GWC17099Leedale_Shallow_3002_17GWC17099397716374.300.0203215716151.04.4
GOWN_Carmangay 2016-03-1550.092178-113.214946insdc:PRJNA861683insdc:SAMN29923108GW3010_BE006_S5_Av6Carmangay301016GWC15095Carmangay_3010_16GWC1509514862390123.100.0203230837265.74.9
GOWN_Morningside 2017-07-1352.550623-113.646106insdc:PRJNA861683insdc:SAMN29923186GW3021_BE090_S47_Av6Morningside302117GWE00067Morningside_3021_17GWE0006751346800.000.02032686841.73.9
GOWN_Rockyford 2016-07-0751.186800-113.242800insdc:PRJNA861683insdc:SAMN29923116GW3026_BE014_S45_Av6Rockyford302616GWC16019Rockyford_3026_16GWC160191315822441.810.4203219921953.14.6
GOWN_Hays_East 2017-12-0949.992354-111.687868insdc:PRJNA861683insdc:SAMN29923213GW3053_BE117_S36_Av6Hays_East305317GWC17068Hays_East_3053_17GWC17068249311600.000.0203211611621.54.0
GOWN_Hamilin 2017-06-2654.002100-112.040000insdc:PRJNA861683insdc:SAMN29923175GW3104_BE079_S46_Av6Hamilin310417GWE00016Hamilin_3104_17GWE000161075510800.000.0203210710741.94.2
GOWN_Warburg 2017-06-2053.126940-114.361390insdc:PRJNA861683insdc:SAMN29923170GW312_BE074_S76_Av6Warburg31217GWE00053Warburg_312_17GWE00053115424500.000.0203237413.01.7
GOWN_Warburg2017-11-0953.126833-114.361333insdc:PRJNA861683insdc:SAMN29923211GW313_BE115_S40_Av6Warburg31317GWE00054Warburg_313_17GWE00054962519552.600.0203217018925.64.1
GOWN_Grimshaw_Nissan 2017-08-2456.246667-117.636667insdc:PRJNA861683insdc:SAMN29923204GW379_BE108_S122_Av6Grimshaw_Nissan37917GWE00086Grimshaw_Nissan_379_17GWE0008620329722.100.02032979726.13.9
GOWN_Meander_River 2017-10-2558.995364-117.656667insdc:PRJNA861683insdc:SAMN29923234GW381_BE138_S73_Av6Meander_River38117GWE00113Meander_River_381_17GWE00113298310932.800.0203210810929.54.0
GOWN_Airdrie 2017-06-0651.234343-114.000246insdc:PRJNA861683insdc:SAMN29923165GW456_BE069_S83_Av6Airdrie45617GWC17008Airdrie_456_17GWC1700849333900.012.6203238393.21.8
GOWN_Canmore_Tourist 2016-09-1651.107306-115.366444insdc:PRJNA861683insdc:SAMN29923135GW760_BE037_S30_Av6Canmore_Tourist76016GWC16051Canmore_Tourist_760_16GWC16051586814721.400.0203213514716.83.6
GOWN_Canmore 2017-08-2151.107306-115.366444insdc:PRJNA861683insdc:SAMN29923200GW760_BE104_S18_Av6Canmore76017GWC17056Canmore_760_17GWC1705624266011.700.0203260603.42.2
GOWN_Okotoks_N2017-01-2550.772353-113.882239insdc:PRJNA861683insdc:SAMN29923158GW787_BE062_S15_Av6Okotoks_N78717GWC16094Okotoks_N_787_17GWC160942557422641.810.420321862129.33.7
GOWN_Moose_Lake 2017-06-1454.276774-110.908469insdc:PRJNA861683insdc:SAMN29923169GW945_BE073_S74_Av6Moose_Lake94517GWE00044Moose_Lake_945_17GWE0004435553800.000.0203237384.62.1
GOWN_Rosebud 2016-09-1151.301531-112.948718insdc:PRJNA861683insdc:SAMN29923153GW972_BE057_S17_Av6Rosebud97216GWC16081Rosebud_972_16GWC1608158342200.000.0203221232.91.6
GOWN_Leedale_Deep 2017-11-1052.537473-114.576872insdc:PRJNA861683insdc:SAMN29923225GW982_BE129_S135_Av6Leedale_Deep98217GWC17097Leedale_Deep_982_17GWC1709714249900.000.02032881.20.3
GOWN_Sundre_South 2017-06-2851.919447-114.560952insdc:PRJNA861683insdc:SAMN29923180GW983_BE084_S60_Av6Sundre_South98317GWC17022Sundre_South_983_17GWC17022218761800.000.0203210141.40.5
GOWN_Sundre_North 2017-06-2851.919462-114.560939insdc:PRJNA861683insdc:SAMN29923179GW984_BE083_S101_Av6Sundre_North98417GWC17020Sundre_North_984_17GWC170207891500.000.02032552.61.1
GOWN_Cochrane_Shallow 2017-08-3151.289625-114.424030insdc:PRJNA861683insdc:SAMN29923207GW985_BE111_S62_Av6Cochrane_Shallow98517GWC17061Cochrane_Shallow_985_17GWC1706124468433.600.02032838512.93.2
GOWN_Cynthia 2017-06-2153.362939-115.590961insdc:PRJNA861683insdc:SAMN29923172GW991_BE076_S140_Av6Cynthia99117GWE00063Cynthia_991_17GWE000635401900.000.02032992.41.1