Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Seawater carbonate chemistry and carapace material properties, cuticle atomic weight composition, elemental concentrations and thickness of juvenile California spiny lobsters (Panulirus interruptus) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.952135
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Abstract:
Spiny lobsters rely on multiple biomineralized exoskeletal predator defenses that may be sensitive to ocean acidification (OA). Compromised mechanical integrity of these defensive structures may tilt predator-prey outcomes, leading to increased mortality in the lobsters' environment. Here, we tested the effects of OA-like conditions on the mechanical integrity of selected exoskeletal defenses of juvenile California spiny lobster, Panulirus interruptus. Young spiny lobsters reside in kelp forests with dynamic carbonate chemistry due to local metabolism and photosynthesis as well as seasonal upwelling, yielding daily and seasonal fluctuations in pH. Lobsters were exposed to a series of stable and diurnally fluctuating reduced pH conditions for three months (ambient pH/stable, 7.97; reduced pH/stable 7.67; reduced pH with low fluctuations, 7.67 ± 0.05; reduced pH with high fluctuations, 7.67 ± 0.10), after which we examined the intermolt composition (Ca and Mg content), ultrastructure (cuticle and layer thickness), and mechanical properties (hardness and stiffness) of selected exoskeletal predator defenses. Cuticle ultrastructure was consistently robust to pH conditions, while mineralization and mechanical properties were variable. Notably, the carapace was less mineralized under both reduced pH treatments with fluctuations, but with no effect on material properties, and the rostral horn had lower hardness in reduced/high fluctuating conditions without a corresponding difference in mineralization. Antennal flexural stiffness was lower in reduced, stable pH conditions compared to the reduced pH treatment with high fluctuations and not correlated with changes in cuticle structure or mineralization. These results demonstrate a complex relationship between mineralization and mechanical properties of the exoskeleton under changing ocean chemistry, and that fluctuating reduced pH conditions can induce responses not observed under the stable reduced pH conditions often used in OA research. Furthermore, this study shows that some juvenile California spiny lobster exoskeletal defenses are responsive to changes in ocean carbonate chemistry, even during the intermolt period, in ways that can potentially increase susceptibility to predation among this critical life stage.
Keyword(s):
Animalia; Arthropoda; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Growth/Morphology; Laboratory experiment; North Pacific; Other; Other studied parameter or process; Panulirus interruptus; Single species; Temperate
Supplement to:
Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification-like conditions. Frontiers in Marine Science, 9, 909017, https://doi.org/10.3389/fmars.2022.909017
Source:
Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.945362
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html
Project(s):
Coverage:
Latitude: 32.853419 * Longitude: -117.268752
Date/Time Start: 2016-10-01T00:00:00 * Date/Time End: 2016-10-01T00:00:00
Event(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-12-12.
Parameter(s):
# | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
---|---|---|---|---|---|---|
1 | Type | Type | Lowder, Kaitlyn | Study | ||
2 | Species, unique identification | Species UID | Lowder, Kaitlyn | |||
3 | Specimen identification | Spec ID | Lowder, Kaitlyn | |||
4 | Status | Status | Lowder, Kaitlyn | molted? | ||
5 | Body region | Body region | Lowder, Kaitlyn | |||
6 | Cuticle layer | Cuticle layer | Lowder, Kaitlyn | |||
7 | Identification | ID | Lowder, Kaitlyn | treatment | ||
8 | Treatment | Treat | Lowder, Kaitlyn | |||
9 | Treatment: pH | T:pH | Lowder, Kaitlyn | |||
10 | pH, standard deviation | pH std dev | ± | Lowder, Kaitlyn | ||
11 | Treatment: temperature | T:temp | °C | Lowder, Kaitlyn | ||
12 | Run Number | Run | # | Lowder, Kaitlyn | ||
13 | Hardness | Hardness | GPa | Lowder, Kaitlyn | Nanoindentation (Nano Hardness Tester, Nanovea, Irvine, CA, USA) | |
14 | Stiffness | Stiffness | GPa | Lowder, Kaitlyn | Nanoindentation (Nano Hardness Tester, Nanovea, Irvine, CA, USA) | |
15 | Validation flag/comment | Flag comm | Lowder, Kaitlyn | Hardness in interquartile range? | ||
16 | Validation flag/comment | Flag comm | Lowder, Kaitlyn | Stiffness in interquartile range? | ||
17 | Comment | Comment | Lowder, Kaitlyn | |||
18 | Carbon | C | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | |
19 | Nitrogen | N | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | |
20 | Oxygen | O | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | |
21 | Sodium | Na | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | |
22 | Magnesium | Mg | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | |
23 | Aluminium | Al | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | |
24 | Silicon | Si | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | |
25 | Phosphorus | P | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | |
26 | Sulfur | S | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | |
27 | Chlorine | Cl | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | |
28 | Calcium | Ca | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | |
29 | Device type | Device | Lowder, Kaitlyn | |||
30 | Quality control | QC | Lowder, Kaitlyn | |||
31 | Carbon | C | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | relative |
32 | Nitrogen | N | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | relative |
33 | Oxygen | O | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | relative |
34 | Sodium | Na | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | relative |
35 | Magnesium | Mg | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | relative |
36 | Aluminium | Al | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | relative |
37 | Silicon | Si | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | relative |
38 | Phosphorus | P | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | relative |
39 | Sulfur | S | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | relative |
40 | Chlorine | Cl | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | relative |
41 | Calcium | Ca | % | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | relative |
42 | Boron-10 | 10B | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
43 | Magnesium-25 | 25Mg | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
44 | Magnesium-26 | 26Mg | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
45 | Magnesium | Mg | µmol/mg | Lowder, Kaitlyn | Weighted average | |
46 | Aluminium-27 | 27Al | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
47 | Phosphorus-31 | 31P | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
48 | Calcium-43 | 43Ca | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
49 | Calcium-48 | 48Ca | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
50 | Calcium | Ca | µmol/mg | Lowder, Kaitlyn | Weighted average | |
51 | Iron-54 | 54Fe | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
52 | Iron-57 | 57Fe | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
53 | Copper-65 | 65Cu | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
54 | Zinc-66 | 66Zn | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
55 | Strontium-86 | 86Sr | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
56 | Barium-137 | 137Ba | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
57 | Lead-208 | 208Pb | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
58 | Uranium-238 | 238U | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
59 | Titanium-48 | 48Ti | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
60 | Chromium-52 | 52Cr | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
61 | Tin-119 | 119Sn | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
62 | Lead-207(I) | 207Pb1 | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
63 | Lead-207(II) | 207Pb2 | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
64 | Lead-207(III) | 207Pb3 | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
65 | Lead-207(IV) | 207Pb4 | µmol/mg | Lowder, Kaitlyn | S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS | |
66 | Distance | Distance | µm | Lowder, Kaitlyn | Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) | |
67 | Validation flag/comment | Flag comm | Lowder, Kaitlyn | |||
68 | Quality control | QC | Lowder, Kaitlyn | |||
69 | Salinity | Sal | Lowder, Kaitlyn | |||
70 | Salinity, standard deviation | Sal std dev | ± | Lowder, Kaitlyn | ||
71 | pH | pH | Lowder, Kaitlyn | Daytime, total scale | ||
72 | pH, standard deviation | pH std dev | ± | Lowder, Kaitlyn | Daytime, total scale | |
73 | Replicates | Repl | # | Lowder, Kaitlyn | ||
74 | Range | Range | Lowder, Kaitlyn | Daily range of pH | ||
75 | Standard deviation | Std dev | ± | Lowder, Kaitlyn | Daily range of pH | |
76 | Replicates | Repl | # | Lowder, Kaitlyn | ||
77 | pH | pH | Lowder, Kaitlyn | Mean, total scale | ||
78 | Temperature, water | Temp | °C | Lowder, Kaitlyn | ||
79 | Temperature, water, standard deviation | Temp std dev | ± | Lowder, Kaitlyn | ||
80 | Replicates | Repl | # | Lowder, Kaitlyn | ||
81 | Carbon, inorganic, dissolved | DIC | µmol/kg | Lowder, Kaitlyn | ||
82 | Carbon, inorganic, dissolved, standard deviation | DIC std dev | ± | Lowder, Kaitlyn | ||
83 | Replicates | Repl | # | Lowder, Kaitlyn | ||
84 | Alkalinity, total | AT | µmol/kg | Lowder, Kaitlyn | ||
85 | Alkalinity, total, standard deviation | AT std dev | ± | Lowder, Kaitlyn | ||
86 | Replicates | Repl | # | Lowder, Kaitlyn | ||
87 | Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) | pCO2water_SST_wet | µatm | Lowder, Kaitlyn | ||
88 | Partial pressure of carbon dioxide, standard deviation | pCO2 std dev | ± | Lowder, Kaitlyn | ||
89 | Replicates | Repl | # | Lowder, Kaitlyn | ||
90 | Bicarbonate ion | [HCO3]- | µmol/kg | Lowder, Kaitlyn | ||
91 | Bicarbonate ion, standard deviation | [HCO3]- std dev | ± | Lowder, Kaitlyn | ||
92 | Replicates | Repl | # | Lowder, Kaitlyn | ||
93 | Calcite saturation state | Omega Cal | Lowder, Kaitlyn | |||
94 | Calcite saturation state, standard deviation | Omega Cal std dev | ± | Lowder, Kaitlyn | ||
95 | Replicates | Repl | # | Lowder, Kaitlyn | ||
96 | Carbonate system computation flag | CSC flag | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | ||
97 | pH | pH | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | total scale | |
98 | Carbon dioxide | CO2 | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | |
99 | Fugacity of carbon dioxide (water) at sea surface temperature (wet air) | fCO2water_SST_wet | µatm | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | |
100 | Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) | pCO2water_SST_wet | µatm | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | |
101 | Bicarbonate ion | [HCO3]- | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | |
102 | Carbonate ion | [CO3]2- | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | |
103 | Aragonite saturation state | Omega Arg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | ||
104 | Calcite saturation state | Omega Cal | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
253607 data points
Download Data
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