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Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Seawater carbonate chemistry and carapace material properties, cuticle atomic weight composition, elemental concentrations and thickness of juvenile California spiny lobsters (Panulirus interruptus) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.952135

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Abstract:
Spiny lobsters rely on multiple biomineralized exoskeletal predator defenses that may be sensitive to ocean acidification (OA). Compromised mechanical integrity of these defensive structures may tilt predator-prey outcomes, leading to increased mortality in the lobsters' environment. Here, we tested the effects of OA-like conditions on the mechanical integrity of selected exoskeletal defenses of juvenile California spiny lobster, Panulirus interruptus. Young spiny lobsters reside in kelp forests with dynamic carbonate chemistry due to local metabolism and photosynthesis as well as seasonal upwelling, yielding daily and seasonal fluctuations in pH. Lobsters were exposed to a series of stable and diurnally fluctuating reduced pH conditions for three months (ambient pH/stable, 7.97; reduced pH/stable 7.67; reduced pH with low fluctuations, 7.67 ± 0.05; reduced pH with high fluctuations, 7.67 ± 0.10), after which we examined the intermolt composition (Ca and Mg content), ultrastructure (cuticle and layer thickness), and mechanical properties (hardness and stiffness) of selected exoskeletal predator defenses. Cuticle ultrastructure was consistently robust to pH conditions, while mineralization and mechanical properties were variable. Notably, the carapace was less mineralized under both reduced pH treatments with fluctuations, but with no effect on material properties, and the rostral horn had lower hardness in reduced/high fluctuating conditions without a corresponding difference in mineralization. Antennal flexural stiffness was lower in reduced, stable pH conditions compared to the reduced pH treatment with high fluctuations and not correlated with changes in cuticle structure or mineralization. These results demonstrate a complex relationship between mineralization and mechanical properties of the exoskeleton under changing ocean chemistry, and that fluctuating reduced pH conditions can induce responses not observed under the stable reduced pH conditions often used in OA research. Furthermore, this study shows that some juvenile California spiny lobster exoskeletal defenses are responsive to changes in ocean carbonate chemistry, even during the intermolt period, in ways that can potentially increase susceptibility to predation among this critical life stage.
Keyword(s):
Animalia; Arthropoda; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Growth/Morphology; Laboratory experiment; North Pacific; Other; Other studied parameter or process; Panulirus interruptus; Single species; Temperate
Supplement to:
Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification-like conditions. Frontiers in Marine Science, 9, 909017, https://doi.org/10.3389/fmars.2022.909017
Source:
Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.945362
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html
Coverage:
Latitude: 32.853419 * Longitude: -117.268752
Date/Time Start: 2016-10-01T00:00:00 * Date/Time End: 2016-10-01T00:00:00
Event(s):
La_Jolla_trap_2016 * Latitude: 32.853419 * Longitude: -117.268752 * Date/Time: 2016-10-01T00:00:00 * Location: off Southern California * Method/Device: Trap, baited (TRAPB)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-12-12.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeLowder, KaitlynStudy
2Species, unique identificationSpecies UIDLowder, Kaitlyn
3Specimen identificationSpec IDLowder, Kaitlyn
4StatusStatusLowder, Kaitlynmolted?
5Body regionBody regionLowder, Kaitlyn
6Cuticle layerCuticle layerLowder, Kaitlyn
7IdentificationIDLowder, Kaitlyntreatment
8TreatmentTreatLowder, Kaitlyn
9Treatment: pHT:pHLowder, Kaitlyn
10pH, standard deviationpH std dev±Lowder, Kaitlyn
11Treatment: temperatureT:temp°CLowder, Kaitlyn
12Run NumberRun#Lowder, Kaitlyn
13HardnessHardnessGPaLowder, KaitlynNanoindentation (Nano Hardness Tester, Nanovea, Irvine, CA, USA)
14StiffnessStiffnessGPaLowder, KaitlynNanoindentation (Nano Hardness Tester, Nanovea, Irvine, CA, USA)
15Validation flag/commentFlag commLowder, KaitlynHardness in interquartile range?
16Validation flag/commentFlag commLowder, KaitlynStiffness in interquartile range?
17CommentCommentLowder, Kaitlyn
18CarbonC%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)
19NitrogenN%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)
20OxygenO%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)
21SodiumNa%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)
22MagnesiumMg%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)
23AluminiumAl%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)
24SiliconSi%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)
25PhosphorusP%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)
26SulfurS%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)
27ChlorineCl%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)
28CalciumCa%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)
29Device typeDeviceLowder, Kaitlyn
30Quality controlQCLowder, Kaitlyn
31CarbonC%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)relative
32NitrogenN%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)relative
33OxygenO%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)relative
34SodiumNa%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)relative
35MagnesiumMg%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)relative
36AluminiumAl%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)relative
37SiliconSi%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)relative
38PhosphorusP%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)relative
39SulfurS%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)relative
40ChlorineCl%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)relative
41CalciumCa%Lowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)relative
42Boron-1010Bµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
43Magnesium-2525Mgµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
44Magnesium-2626Mgµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
45MagnesiumMgµmol/mgLowder, KaitlynWeighted average
46Aluminium-2727Alµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
47Phosphorus-3131Pµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
48Calcium-4343Caµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
49Calcium-4848Caµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
50CalciumCaµmol/mgLowder, KaitlynWeighted average
51Iron-5454Feµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
52Iron-5757Feµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
53Copper-6565Cuµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
54Zinc-6666Znµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
55Strontium-8686Srµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
56Barium-137137Baµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
57Lead-208208Pbµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
58Uranium-238238Uµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
59Titanium-4848Tiµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
60Chromium-5252Crµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
61Tin-119119Snµmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
62Lead-207(I)207Pb1µmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
63Lead-207(II)207Pb2µmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
64Lead-207(III)207Pb3µmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
65Lead-207(IV)207Pb4µmol/mgLowder, KaitlynS-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS
66DistanceDistanceµmLowder, KaitlynScanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX)
67Validation flag/commentFlag commLowder, Kaitlyn
68Quality controlQCLowder, Kaitlyn
69SalinitySalLowder, Kaitlyn
70Salinity, standard deviationSal std dev±Lowder, Kaitlyn
71pHpHLowder, KaitlynDaytime, total scale
72pH, standard deviationpH std dev±Lowder, KaitlynDaytime, total scale
73ReplicatesRepl#Lowder, Kaitlyn
74RangeRangeLowder, KaitlynDaily range of pH
75Standard deviationStd dev±Lowder, KaitlynDaily range of pH
76ReplicatesRepl#Lowder, Kaitlyn
77pHpHLowder, KaitlynMean, total scale
78Temperature, waterTemp°CLowder, Kaitlyn
79Temperature, water, standard deviationTemp std dev±Lowder, Kaitlyn
80ReplicatesRepl#Lowder, Kaitlyn
81Carbon, inorganic, dissolvedDICµmol/kgLowder, Kaitlyn
82Carbon, inorganic, dissolved, standard deviationDIC std dev±Lowder, Kaitlyn
83ReplicatesRepl#Lowder, Kaitlyn
84Alkalinity, totalATµmol/kgLowder, Kaitlyn
85Alkalinity, total, standard deviationAT std dev±Lowder, Kaitlyn
86ReplicatesRepl#Lowder, Kaitlyn
87Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmLowder, Kaitlyn
88Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Lowder, Kaitlyn
89ReplicatesRepl#Lowder, Kaitlyn
90Bicarbonate ion[HCO3]-µmol/kgLowder, Kaitlyn
91Bicarbonate ion, standard deviation[HCO3]- std dev±Lowder, Kaitlyn
92ReplicatesRepl#Lowder, Kaitlyn
93Calcite saturation stateOmega CalLowder, Kaitlyn
94Calcite saturation state, standard deviationOmega Cal std dev±Lowder, Kaitlyn
95ReplicatesRepl#Lowder, Kaitlyn
96Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
97pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
98Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
99Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
100Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
101Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
102Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
103Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
104Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
253607 data points

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