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Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Carapace properties of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification - files to run with R code [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.945372

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Abstract:
We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.2687516) in October 2016 and held at ambient conditions (pH 7.97, 16.5℃) before exposure to stable or diurnally fluctuating reduced pH conditions established by bubbling CO2 and as measured using best practices (ambient pH/stable, 7.97, 16.5℃; reduced pH/stable 7.67, 16.6℃; reduced pH with low fluctuations, 7.67 ± 0.05, 16.4℃; reduced pH with high fluctuations, 7.67 ± 0.10, 16.4℃). After three months, we examined the composition (wt. % and concentration of Ca and Mg), ultrastructure (cuticle and thickness of exocuticle ), and mechanical properties (hardness and stiffness). Layer thickness (µm) and wt. % mineralization were determined using a scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX). Concentration of elements (µmol/mg sample) was measured using inductively-coupled x-ray spectrometry (ICP-MS), while material properties (GPa) were measured using a nanoindenter with a Berkovitch diamond tip. The metadata file describes headers, units, methods, etc in each data file. CSV files are data files for separate measurements and will run with R code stored at GitHub to reproduce analyses and figures.
Keyword(s):
California Current Ecosystem; crustacean; Laboratory experiment; Ocean acidification
Supplement to:
Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification-like conditions. Frontiers in Marine Science, 9, 909017, https://doi.org/10.3389/fmars.2022.909017
Related to:
Lowder, Kaitlyn (2022): kblowder/Spiny-lobster-exoskeleton-OA: code only. Zenodo, https://doi.org/10.5281/zenodo.6596558
Other version:
Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification. PANGAEA, https://doi.org/10.1594/PANGAEA.945362
Coverage:
Latitude: 32.853419 * Longitude: -117.268752
Date/Time Start: 2016-10-01T00:00:00 * Date/Time End: 2016-10-01T00:00:00
Event(s):
La_Jolla_trap_2016 * Latitude: 32.853419 * Longitude: -117.268752 * Date/Time: 2016-10-01T00:00:00 * Location: off Southern California * Method/Device: Trap, baited (TRAPB)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Binary ObjectBinaryLowder, Kaitlyn
Status:
Curation Level: Basic curation (CurationLevelB)
Size:
5 data points

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