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Johnson, Roberta; Langer, Gerald; Rossi, Sergio; Probert, Ian; Mammone, Marta; Ziveri, Patrizia (2022): Seawater carbonate chemistry and lipid content, particulate organic carbon/nitrogen, growth rate, and morphology of Emiliania huxleyi [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.949280

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Abstract:
Coccolithophores are a calcifying unicellular phytoplankton group that are at the base of the marine food web, and their lipid content provides a source of energy to consumers. Coccolithophores are vulnerable to ocean acidification and warming, therefore it is critical to establish the effects of climate change on these significant marine primary producers, and determine potential consequences that these changes can have on their consumers. Here, we quantified the impact of changes in pH and temperature on the nutritional condition (lipid content, particulate organic carbon/nitrogen), growth rate, and morphology of the most abundant living coccolithophore species, Emiliania huxleyi. We used a regression type approach with nine pH levels (ranging from 7.66 to 8.44) and two temperatures (15°C and 20°C). Lipid production was greater under reduced pH, and growth rates were distinctly lower at 15°C than at 20°C. The production potential of lipids, which estimates the availability of lipids to consumers, increased under 20°C, but decreased under low pH. The results indicate that, while consumers will benefit energetically under ocean warming, this benefit will be mitigated by ocean acidification. The carbon to nitrogen ratio was higher at 20°C and low pH, indicating that the nutritional quality of coccolithophores for consumers will decline under climate change. The impact of low pH on the structural integrity of the coccosphere may also mean that coccolithophores are easier to digest for consumers. Many responses suggest cellular stress, indicating that increases in temperature and reductions in pH may have a negative impact on the ecophysiology of coccolithophores.
Keyword(s):
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Temperature
Supplement to:
Johnson, Roberta; Langer, Gerald; Rossi, Sergio; Probert, Ian; Mammone, Marta; Ziveri, Patrizia (2022): Nutritional response of a coccolithophore to changing pH and temperature. Limnology and Oceanography, lno.12204, https://doi.org/10.1002/lno.12204
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-10-04.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeJohnson, RobertaStudy
2Species, unique identificationSpecies UIDJohnson, Roberta
3Species, unique identification (URI)Species UID (URI)Johnson, Roberta
4Species, unique identification (Semantic URI)Species UID (Semantic URI)Johnson, Roberta
5Temperature, waterTemp°CJohnson, Roberta
6pH, total scalepHTJohnson, Robertatotal scale
7Alkalinity, totalATµmol/kgJohnson, Roberta
8Alkalinity, total, standard deviationAT std dev±Johnson, Roberta
9Growth rateµ1/dayJohnson, Roberta
10Growth rate, standard deviationµ std dev±Johnson, Roberta
11Coccosphere, diameterCoccosp diamµmJohnson, Roberta
12Coccosphere, diameter, standard deviationCoccosp diam std dev±Johnson, Roberta
13Distal shield, lengthDistal shield LµmJohnson, Roberta
14Distal shield, length, standard deviationDistal shield L std dev±Johnson, Roberta
15Distal shield, widthDistal shield wµmJohnson, Roberta
16Distal shield, width, standard deviationDistal shield w std dev±Johnson, Roberta
17DiameterصmJohnson, RobertaInner circle
18Diameter, standard deviationØ std dev±Johnson, RobertaInner circle
19WidthwµmJohnson, Robertatube
20Width, standard deviationw std dev±Johnson, Robertatube
21CoccospheresCoccosp%Johnson, RobertaCollapsed
22Lipids per cellLipids/cellpg/#Johnson, Roberta
23Lipids, standard deviationLipids std dev±Johnson, Roberta
24Lipid production per cellLipid prod/cellpg/#/dayJohnson, Roberta
25Lipid production per cell, standard deviationLipid prod/cell std dev±Johnson, Roberta
26Lipid production per cellLipid prod/cellpg/#/dayJohnson, Robertapotential Lipids
27Lipid production per cell, standard deviationLipid prod/cell std dev±Johnson, Robertapotential Lipids
28Carbon, organic, particulate/Nitrogen, particulate ratioPOC/PNJohnson, Roberta
29Carbon, organic, particulate/Nitrogen, particulate ratio, standard deviationPOC/PN std dev±Johnson, Roberta
30Particulate inorganic carbon/total particulate nitrogen ratioPIC/TPNJohnson, Roberta
31Particulate inorganic carbon/total particulate nitrogen ratio, standard deviationPIC/TPN std dev±Johnson, Roberta
32Particulate inorganic carbon/particulate organic carbon ratioPIC/POCJohnson, Roberta
33Particulate inorganic carbon/particulate organic carbon ratio, standard deviationPIC/POC ratio std dev±Johnson, Roberta
34Cellular lipid quota/cellular particulate organic carbon quotaLipid/POCJohnson, Roberta
35Cellular lipid quota/cellular particulate organic carbon quota, standard deviationLipid/POC std dev±Johnson, Roberta
36Carbon, inorganic, particulate, per cellPIC/cellpg/#Johnson, Roberta
37Carbon, inorganic, particulate, standard deviationPIC std dev±Johnson, Roberta
38Carbon, inorganic, particulate, production per cellPIC prod/cellpg/#/dayJohnson, Roberta
39Particulate inorganic carbon, production, standard deviationPIC prod std dev±Johnson, Roberta
40Carbon, organic, particulate, per cellPOC/cellpg/#Johnson, Roberta
41Carbon, organic, particulate, standard deviationPOC std dev±Johnson, Roberta
42Carbon, organic, particulate, production per cellPOC prod/cellpg/#/dayJohnson, Roberta
43Particulate organic carbon, production, standard deviationPOC prod std dev±Johnson, Roberta
44Chlorophyll a per cellChl a/cellpg/#Johnson, Roberta
45Chlorophyll a, standard deviationChl a std dev±Johnson, Roberta
46Chlorophyll a production per cellChl a prod/cellpg/#/dayJohnson, Roberta
47Chlorophyll a, production, standard deviationChl a prod±Johnson, Roberta
48SalinitySalJohnson, Roberta
49Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
50Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
51Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
52Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
53Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
54Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
55Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
56Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
57Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
979 data points

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