Citation:

PANGAEA.949280

Name: Seawater carbonate chemistry and lipid content, particulate organic carbon/nitrogen, growth rate, and morphology of Emiliania huxleyi
Published: 2022-10-04
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Temperature

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Abstract:

Coccolithophores are a calcifying unicellular phytoplankton group that are at the base of the marine food web, and their lipid content provides a source of energy to consumers. Coccolithophores are vulnerable to ocean acidification and warming, therefore it is critical to establish the effects of climate change on these significant marine primary producers, and determine potential consequences that these changes can have on their consumers. Here, we quantified the impact of changes in pH and temperature on the nutritional condition (lipid content, particulate organic carbon/nitrogen), growth rate, and morphology of the most abundant living coccolithophore species, Emiliania huxleyi. We used a regression type approach with nine pH levels (ranging from 7.66 to 8.44) and two temperatures (15°C and 20°C). Lipid production was greater under reduced pH, and growth rates were distinctly lower at 15°C than at 20°C. The production potential of lipids, which estimates the availability of lipids to consumers, increased under 20°C, but decreased under low pH. The results indicate that, while consumers will benefit energetically under ocean warming, this benefit will be mitigated by ocean acidification. The carbon to nitrogen ratio was higher at 20°C and low pH, indicating that the nutritional quality of coccolithophores for consumers will decline under climate change. The impact of low pH on the structural integrity of the coccosphere may also mean that coccolithophores are easier to digest for consumers. Many responses suggest cellular stress, indicating that increases in temperature and reductions in pH may have a negative impact on the ecophysiology of coccolithophores.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Temperature

Supplement to:

Johnson, Roberta; Langer, Gerald; Rossi, Sergio; Probert, Ian; Mammone, Marta; Ziveri, Patrizia (2022): Nutritional response of a coccolithophore to changing pH and temperature. Limnology and Oceanography, lno.12204, https://doi.org/10.1002/lno.12204

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-10-04.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Johnson, Roberta		Study
2	Species, unique identification	Species UID		Johnson, Roberta
3	Species, unique identification (URI)	Species UID (URI)		Johnson, Roberta
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Johnson, Roberta
5	Temperature, water	Temp	°C	Johnson, Roberta
6	pH	pH		Johnson, Roberta		total scale
7	Alkalinity, total	AT	µmol/kg	Johnson, Roberta
8	Alkalinity, total, standard deviation	AT std dev	±	Johnson, Roberta
9	Growth rate	µ	1/day	Johnson, Roberta
10	Growth rate, standard deviation	µ std dev	±	Johnson, Roberta
11	Coccosphere, diameter	Coccosp diam	µm	Johnson, Roberta
12	Coccosphere, diameter, standard deviation	Coccosp diam std dev	±	Johnson, Roberta
13	Distal shield, length	Distal shield L	µm	Johnson, Roberta
14	Distal shield, length, standard deviation	Distal shield L std dev	±	Johnson, Roberta
15	Distal shield, width	Distal shield w	µm	Johnson, Roberta
16	Distal shield, width, standard deviation	Distal shield w std dev	±	Johnson, Roberta
17	Diameter	Ø	µm	Johnson, Roberta		Inner circle
18	Diameter, standard deviation	Ø std dev	±	Johnson, Roberta		Inner circle
19	Width	w	µm	Johnson, Roberta		tube
20	Width, standard deviation	w std dev	±	Johnson, Roberta		tube
21	Coccospheres	Coccosp	%	Johnson, Roberta		Collapsed
22	Lipids per cell	Lipids/cell	pg/#	Johnson, Roberta
23	Lipids, standard deviation	Lipids std dev	±	Johnson, Roberta
24	Lipid production per cell	Lipid prod/cell	pg/#/day	Johnson, Roberta
25	Lipid production per cell, standard deviation	Lipid prod/cell std dev	±	Johnson, Roberta
26	Lipid production per cell	Lipid prod/cell	pg/#/day	Johnson, Roberta		potential Lipids
27	Lipid production per cell, standard deviation	Lipid prod/cell std dev	±	Johnson, Roberta		potential Lipids
28	Carbon, organic, particulate/Nitrogen, particulate ratio	POC/PN		Johnson, Roberta
29	Carbon, organic, particulate/Nitrogen, particulate ratio, standard deviation	POC/PN std dev	±	Johnson, Roberta
30	Particulate inorganic carbon/total particulate nitrogen ratio	PIC/TPN		Johnson, Roberta
31	Particulate inorganic carbon/total particulate nitrogen ratio, standard deviation	PIC/TPN std dev	±	Johnson, Roberta
32	Particulate inorganic carbon/particulate organic carbon ratio	PIC/POC		Johnson, Roberta
33	Particulate inorganic carbon/particulate organic carbon ratio, standard deviation	PIC/POC ratio std dev	±	Johnson, Roberta
34	Cellular lipid quota/cellular particulate organic carbon quota	Lipid/POC		Johnson, Roberta
35	Cellular lipid quota/cellular particulate organic carbon quota, standard deviation	Lipid/POC std dev	±	Johnson, Roberta
36	Carbon, inorganic, particulate, per cell	PIC/cell	pg/#	Johnson, Roberta
37	Carbon, inorganic, particulate, standard deviation	PIC std dev	±	Johnson, Roberta
38	Particulate inorganic carbon production per cell	PIC prod/cell	pg/#/day	Johnson, Roberta
39	Particulate inorganic carbon, production, standard deviation	PIC prod std dev	±	Johnson, Roberta
40	Carbon, organic, particulate, per cell	POC/cell	pg/#	Johnson, Roberta
41	Carbon, organic, particulate, standard deviation	POC std dev	±	Johnson, Roberta
42	Particulate organic carbon production per cell	POC prod/cell	pg/#/day	Johnson, Roberta
43	Particulate organic carbon, production, standard deviation	POC prod std dev	±	Johnson, Roberta
44	Chlorophyll a per cell	Chl a/cell	pg/#	Johnson, Roberta
45	Chlorophyll a, standard deviation	Chl a std dev	±	Johnson, Roberta
46	Chlorophyll a production per cell	Chl a prod/cell	pg/#/day	Johnson, Roberta
47	Chlorophyll a, production, standard deviation	Chl a prod	±	Johnson, Roberta
48	Salinity	Sal		Johnson, Roberta
49	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
54	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
55	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
56	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
57	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

979 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (Study)	2 Species UID	3 Species UID (URI)	4 Species UID (Semantic URI)	5 Temp [°C]	6 pH (total scale)	7 AT [µmol/kg]	8 AT std dev [±]	9 µ [1/day]	10 µ std dev [±]	11 Coccosp diam [µm]	12 Coccosp diam std dev [±]	13 Distal shield L [µm]	14 Distal shield L std dev [±]	15 Distal shield w [µm]	16 Distal shield w std dev [±]	17 Ø [µm] (Inner circle)	18 Ø std dev [±] (Inner circle)	19 w [µm] (tube)	20 w std dev [±] (tube)	21 Coccosp [%] (Collapsed)	22 Lipids/cell [pg/#]	23 Lipids std dev [±]	24 Lipid prod/cell [pg/#/day]	25 Lipid prod/cell std dev [±]	26 Lipid prod/cell [pg/#/day] (potential Lipids)	27 Lipid prod/cell std dev [±] (potential Lipids)	28 POC/PN	29 POC/PN std dev [±]	30 PIC/TPN	31 PIC/TPN std dev [±]	32 PIC/POC	33 PIC/POC ratio std dev [±]	34 Lipid/POC	35 Lipid/POC std dev [±]	36 PIC/cell [pg/#]	37 PIC std dev [±]	38 PIC prod/cell [pg/#/day]	39 PIC prod std dev [±]	40 POC/cell [pg/#]	41 POC std dev [±]	42 POC prod/cell [pg/#/day]	43 POC prod std dev [±]	44 Chl a/cell [pg/#]	45 Chl a std dev [±]	46 Chl a prod/cell [pg/#/day]	47 Chl a prod [±]	48 Sal	49 CSC flag	50 CO2 [µmol/kg]	51 fCO2water_SST_wet [µatm]	52 pCO2water_SST_wet [µatm]	53 [HCO3]- [µmol/kg]	54 [CO3]2- [µmol/kg]	55 DIC [µmol/kg]	56 Omega Arg	57 Omega Cal
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	15	7.66	2194.7	0.32	0.61	0.02	6.60	0.65	3.46	0.35	3.03	0.28	1.73	0.24	0.22	0.06	44.14	44.72	17.18	27.22	10.46	27.22	10.46	6.2	0.11	3.58	1.57	0.33	0.09	1.70	0.16	8.69	2.40	5.29	1.46	26.28	0.24	15.99	0.15	0.27	0.01	0.16	0.01	35	8	38.54	1028.83	1032.56	2022.71	68.70	2129.94	1.05	1.64
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	15	7.84	2170.1	2.03	0.61	0.02	6.99	0.83	3.72	0.44	3.23	0.37	1.65	0.24	0.30	0.07	43.78	48.33	5.72	29.50	3.49	29.50	3.49	6.4	0.04	3.72	0.33	0.40	0.04	1.98	0.06	9.68	0.85	5.91	0.52	24.40	0.23	14.89	0.14	0.23	0.02	0.14	0.01	35	8	24.20	645.93	648.28	1922.11	98.80	2045.11	1.51	2.36
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	15	7.92	2187.9	1.53	0.66	0.01	7.04	0.70	3.76	0.34	3.27	0.39	1.65	0.19	0.30	0.07	66.94	59.16	6.05	39.07	4.00	39.07	4.00	6.5	0.09	3.60	0.35	0.47	0.01	2.06	0.05	13.51	0.00	8.92	0.00	28.74	0.32	18.98	0.21	0.31	0.02	0.20	0.02	35	8	19.84	529.55	531.47	1894.52	117.08	2031.44	1.79	2.79
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	15	8.01	2214.9	6.84	0.65		7.31	0.75	3.84	0.34	3.31	0.35	1.67	0.19	0.32	0.08	38.10	53.52	5.26	34.66	3.41	34.66	3.41	6.4	0.09	3.31	0.08	0.47	0.07	1.79	0.04	14.02	1.70	9.08	1.10	29.88	0.96	19.35	0.62	0.31	0.04	0.20	0.02	35	8	15.84	422.86	424.40	1861.19	141.51	2018.54	2.17	3.37
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	15	8.06	2257.8	3.70	0.65	0.02	7.10	0.73	3.87	0.37	3.34	0.41	1.71	0.25	0.31	0.08	33.33	49.76	10.17	32.55	6.65	32.55	6.65	6.0	0.25	2.85	0.14	0.57	0.09	1.78	0.09	15.79	1.76	10.33	1.15	27.92	1.14	18.26	0.75	0.29	0.00	0.19	0.00	35	8	14.13	377.09	378.46	1862.25	158.87	2035.24	2.43	3.79
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	15	8.17	2307.8	0.55	0.65	0.01	6.63	0.71	3.91	0.37	3.38	0.33	1.73	0.23	0.32	0.09	24.41	42.15	8.70	27.40	5.66	27.40	5.66	5.6	0.10	2.83	0.24	0.66	0.05	1.73	0.09	16.02	0.95	10.41	0.61	24.33	0.30	15.82	0.20	0.27	0.02	0.18	0.02	35	8	10.68	285.08	286.12	1813.69	199.32	2023.69	3.05	4.75
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	15	8.23	2355.0	1.37	0.67	0.02	6.83	0.78	3.95	0.40	3.43	0.41	1.65	0.26	0.34	0.10	27.73	48.73	8.34	32.61	5.58	32.61	5.58	5.3	0.15	2.96	0.27	0.69	0.01	2.32	0.10	14.50	0.19	9.71	0.13	21.05	0.00	14.08	0.00	0.22	0.01	0.15	0.00	35	8	9.21	245.82	246.71	1795.60	226.57	2031.38	3.47	5.40
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	15	8.33	2467.3	2.86	0.55	0.01	6.96	0.78	3.94	0.36	3.39	0.37	1.60	0.42	0.38	0.19	18.02	46.63	3.30	25.58	1.81	25.58	1.81	5.0	0.16	3.48	0.65	0.56	0.03	1.75	0.03	15.00	0.64	8.23	0.35	26.72	0.16	14.66	0.09	0.28	0.04	0.15	0.02	35	8	7.24	193.21	193.91	1776.74	282.24	2066.22	4.32	6.73
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	15	8.43	2572.5	3.93	0.56	0.03	6.46	0.64	3.70	0.37	3.66	0.35	1.57	0.30	0.34	0.12	20.92	35.95	6.62	20.19	3.72	20.19	3.72	4.9	0.11	2.00		0.28		1.68	0.07	2.74		3.08		21.41	2.80	12.02	1.57	0.28	0.04	0.12	0.02	35	8	5.60	149.59	150.13	1731.79	346.33	2083.73	5.30	8.26
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	20	7.74	2179.0	1.14	0.75	0.01	6.25	0.61	3.62	0.43	3.12	0.47	1.67	0.28	0.30	0.11	67.23	61.34	0.95	45.91	0.71	45.91	0.71	9.2	0.59	3.15		0.38		3.25	0.01	7.09		10.62		18.90		28.29		0.83	0.18	0.62	0.13	35	8	27.49	848.40	851.29	1940.38	95.79	2063.66	1.49	2.29
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	20	7.90	2155.0	0.07	0.80	0.01	6.54	0.74	3.69	0.46	3.19	0.44	1.68	0.21	0.30	0.10	46.73	40.66	3.48	32.72	2.80	32.72	2.80	8.1	0.16	3.45	0.40	0.45	0.02	1.42	0.03	12.87	0.28	10.36	0.22	28.65	0.42	23.06	0.34	0.45	0.01	0.36	0.01	35	8	17.93	553.25	555.13	1828.95	130.51	1977.39	2.03	3.12
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	20	7.97	2203.7	6.73	0.84	0.03	6.12	0.91	3.49	0.45	2.86	0.45	1.51	0.29	0.31	0.10	33.90	55.01	2.81	46.31	2.37	46.31	2.37	7.5	0.95	3.69	0.06	0.53	0.03	1.37	0.02	21.34	0.91	17.97	0.77	40.28	0.66	33.91	0.56	0.61	0.05	0.51	0.04	35	8	15.21	469.46	471.06	1823.40	152.87	1991.49	2.38	3.66
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	20	8.09	2101.2	1.05	0.82	0.01	6.67	0.85	3.54	0.40	3.02	0.39	1.57	0.20	0.33	0.07	23.78	47.71	3.74	39.34	3.08	39.34	3.08	7.3	0.11	3.87	0.60	0.52	0.04	1.41	0.03	17.68	1.52	14.58	1.26	33.79	0.32	27.87	0.26	0.47	0.02	0.39	0.02	35	8	10.41	321.14	322.23	1644.28	181.73	1836.42	2.83	4.35
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	20	8.18	2157.8	0.06	0.92	0.02	6.81	0.98	3.44	0.39	2.97	0.45	1.54	0.19	0.32	0.06	35.59	38.45	2.98	35.52	2.76	35.52	2.76	6.2	0.60	3.37	0.87	0.50	0.18	1.19	0.02	15.85	3.65	14.64	3.38	32.34	4.34	29.87	4.01	0.35	0.02	0.33	0.02	35	8	8.28	255.60	256.47	1610.08	218.93	1837.29	3.40	5.24
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	20	8.29	2250.5	0.04	0.92	0.01	7.12	0.95	3.56	0.41	3.02	0.36	1.60	0.22	0.33	0.06	27.74	38.87	0.67	35.70	0.61	35.70	0.61	5.7	0.19	4.15	0.58	0.57	0.02	1.44	0.01	15.36	0.25	14.11	0.23	26.97	0.60	24.78	0.55	0.32	0.04	0.29	0.03	35	8	6.26	193.29	193.94	1568.49	274.75	1849.51	4.27	6.57
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	20	8.29	2385.8	2.48	0.84	0.01	6.86	1.06	3.58	0.32	3.08	0.35	1.62	0.18	0.32	0.07	40.37	35.62	6.82	30.05	5.76	30.05	5.76	5.3	0.08	4.08	0.28	0.56	0.04	1.32	0.06	14.95	1.33	12.62	1.12	26.89	0.31	22.69	0.26	0.36	0.03	0.30	0.02	35	8	6.66	205.63	206.33	1668.69	292.30	1967.66	4.54	6.99
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	20	8.38	2529.3	0.11	0.85	0.02	6.62	0.91	3.73	0.34	3.15	0.38	1.69	0.20	0.35	0.07	24.03	29.55	3.45	25.10	2.93	25.10	2.93	5.5	0.11	4.80	0.21	0.51	0.05	1.53	0.04	9.82	0.73	8.34	0.62	19.29	0.35	16.38	0.30	0.28	0.01	0.24	0.01	35	8	5.39	166.33	166.89	1660.54	357.85	2023.79	5.56	8.56
laboratory	Emiliania huxleyi	marinespecies.org	urn:lsid:marinespecies.org:taxname:115104	20	8.44	2695.8	1.38	0.98	0.03	6.52	0.94	3.47	0.34	2.86	0.42	1.65	0.19	0.32	0.07	47.23	29.42	6.00	28.76	5.87	28.76	5.87	5.9	0.13	3.34	0.61	0.31	0.03	1.45	0.07	6.34	0.05	6.20	0.04	20.36	1.64	19.90	1.60	0.30	0.05	0.29	0.05	35	8	4.78	147.54	148.04	1691.22	418.46	2114.45	6.51	10.01