Fischer, Jonas; Talal, Ghanem; Schnee, Laura Sophie; Otomo, Patricks; Filser, Juliane (2022): Moulting test on the springtail Folsomia candida exposed via artificial test soils in a laboratory experiment [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.949199, In: Fischer, J et al. (2022): Physiological, molecular and behavioural effects of copper oxide nanoparticles and copper chloride on the springtail Folsomia candida exposed via artificial test soils in a laboratory experiment [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.949202
Always quote citation above when using data! You can download the citation in several formats below.
Abstract:
In a laboratory experiment, the soil invertebrate Folsomia candida (Collembola) was exposed to CuCl2 or copper oxide nanoparticles (CuO-NP) for 6 days. The animals were exposed via artificial test soil which varied in the size (19 or 30% of total mass) or the clay type of their clay fraction (kaolin or montmorillonite). The test substances were dissolved (CuCl2) or dispersed (CuO-NP) in pure miliQ water and stirred into the dry test soils to end up with test concentrations of 1, 3, 10 and 32 mg Cu/kg and a water content of 50% of the maximum water holding capacity of the respective soils. As a control, soil with miliQ water only was tested. For all soils, two tests with 5 replicates per treatment were conducted. At the beginning of the experiment, 30 animals at the age of 10-12 days were inserted into the test vessels containing the spiked test soil. After 6 days, all animals were extracted by floatation of the test soils with tap water and transferred to a Paris-plaster culture plate. After 2, 5 and 7 days, the exuviae shed by the animals were collected under a binocular and analysed for their total Cu content (not per biomass as mass of exuviae is too little to be measured) via atomic absorption spectroscopy (AAS). The number of shed exuviae per animal was calculated for each time point and statistically compared to the control by a linear mixed-effect model considering the two test runs as random factor. The Cu contents of the samples were analysed by an ordinal regression with respect to their ability to exceed the limit of detection or quantification in the AAS.
Keyword(s):
Comment:
Funded by :
Hans‐Böckler‐Stiftung within the framework of the graduate school NanoCompetence (PK041). Open Access funding enabled and organized by Projekt DEAL.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experiment | Exp | Fischer, Jonas | Test name | ||
| 2 | Soil name | Soil name | Fischer, Jonas | Laboratory experiment | Test soil | |
| 3 | Treatment | Treat | Fischer, Jonas | Laboratory experiment | Treatment: test substance (NP, S) and the soil concentration of the respective Cu compound (1, 3, 10 or 32 mg Cu/kg), eg 3 mg Cu/kg with CuO-NP = NP-3; control (C). | |
| 4 | Test substance | Test subs | Fischer, Jonas | Laboratory experiment | Test substances: CuCl2 (S) and CuO-NP (NP), control (C) only contains miliQ water | |
| 5 | Copper, per soil dry mass | Cu soil dm | mg/kg | Fischer, Jonas | Laboratory experiment | |
| 6 | Replicates | Repl | # | Fischer, Jonas | Laboratory experiment | |
| 7 | Number of individuals | Ind No | # | Fischer, Jonas | Laboratory experiment | Used individuals |
| 8 | Time of day | Time of day | Fischer, Jonas | Laboratory experiment | a = 2 days, b = 5 days, d = 7 days | |
| 9 | Folsomia candida, exuviae | F. candida exuviae | # | Fischer, Jonas | Laboratory experiment | |
| 10 | Folsomia candida, exuviae, cumulated | F. candida exuviae cum | # | Fischer, Jonas | Laboratory experiment | |
| 11 | Folsomia candida, exuviae | F. candida exuviae | # | Fischer, Jonas | Laboratory experiment | per individual |
| 12 | Folsomia candida, exuviae, cumulated | F. candida exuviae cum | # | Fischer, Jonas | Laboratory experiment | per individual |
| 13 | Copper | Cu | ng | Fischer, Jonas | Laboratory experiment | |
| 14 | Sample comment | Sample comment | Fischer, Jonas | Laboratory experiment | Data censoring criteria for Cu content |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
6988 data points
Download Data
View dataset as HTML (shows only first 2000 rows)