Carreiro-Silva, Marina; Martins, Ines; Raimundo, Joana; Caetano, Miguel; Bettencourt, Raul; Cerqueira, Teresa; Colaço, Ana (2022): Gene expression by the cold-water octocoral Dentomuricea aff. meteor during an ex-situ experiment testing the effects of mining-generated sediment plumes [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.948413, In: Carreiro-Silva, M et al. (2022): Results of an ex-situ experiment testing the effects of mining-generated sediment plumes on the cold-water octocoral Dentomuricea aff. meteor in the Azores [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.948414
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Published: 2022-09-15 • DOI registered: 2022-12-17
Abstract:
We report the results of an aquaria-based experiment testing the effects of suspended particles generated during potential mining activities, on a common habitat-building coral species in the Azores, Dentomuricea aff. meteor. Coral fragments were maintained in 10-L aquaria and exposed to three experimental treatments for a period of four weeks at the DeepSeaLab aquaria facilities (Okeanos-University of the Azores): (1) control conditions (no added sediments); (2) suspended polymetallic sulphide (PMS) particles; (3) suspended quartz particles. Gene expression profiles in D. aff. meteor were used to evaluate the physiological pathways involved in the response to exposure to PMS and quartz particles. Coral fragments were collected from each treatment at times 0, 3, and 13 days and for the control and quartz treatments also at time 27 days. The study targeted genes involved in cellular stress and antioxidant reaction system (heat shock protein, superoxide dismutase, ferritin), cell structure/integrity (α-carbonic anhydrase, receptor-type protein tyrosine phosphatase) and immune responses (toll-like receptor, lysozyme, rel homology domain, ferritin).
Keyword(s):
Related to:
Carreiro-Silva, Marina; Martins, Ines; Riou, Virginie; Raimundo, Joana; Caetano, Miguel; Bettencourt, Raul; Rakka, Maria; Cerqueira, Teresa; Godinho, Antonio; Morato, Telmo; Colaço, Ana (2022): Mechanical and toxicological effects of deep-sea mining sediment plumes on a habitat-forming cold-water octocoral. Frontiers in Marine Science, 9, 915650, https://doi.org/10.3389/fmars.2022.915650
Project(s):
Funding:
Horizon 2020 (H2020), grant/award no. 678760: A Trans-Atlantic assessment and deep-water ecosystem-based spatial management plan for Europe
Horizon 2020 (H2020), grant/award no. 818123: Integrated Assessment of Atlantic Marine Ecosystems in Space and Time
Seventh Framework Programme (FP7), grant/award no. 603418: Managing Impacts of Deep-seA reSource exploitation
Coverage:
Latitude: 38.539689 * Longitude: -29.039155
Date/Time Start: 2014-08-08T00:00:00 * Date/Time End: 2014-08-08T00:00:00
Minimum Elevation: -210.0 m * Maximum Elevation: -185.0 m
Event(s):
Sampling_Azores_Cold_Water_Octocoral * Latitude: 38.539689 * Longitude: -29.039155 * Date/Time: 2014-08-08T00:00:00 * Elevation Start: -185.0 m * Elevation End: -210.0 m * Location: Condor Seamounts * Comment: Sampling location of octoral specimens, Dentomuricea aff. meteor, studied in ex-situ aquaria experiments at the DeepSeaLab aquaria facilities of the Okeanos Institute, University of the Azores.
Comment:
Gene expression profiles in D. aff. meteor exposed to the different treatments
HKG: House Keeping Genes (18S and β-actin)
RHD: rel homology domain
TLR: toll-like receptor
HSP70: heat shock protein
RP Tyr-PH: receptor-type protein tyrosine phosphatase
SOD: superoxide dismutase
Carb Anhy: α-carbonic anhydrase
Ct = cycle threshold (nº of cycles in an RT-PCR required for the signal to exceed the background level)
Relative gene expression of the gene = Relative quantity gene / Geometric mean of the relative quantities of each housekeeping gene (=RQ gene/Geomean RQ HKGs)
Relative quantity (RQ) = (efficiency of the gene)^ΔCT
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Gene name | Gene | Carreiro-Silva, Marina | |||
| 2 | Specimen identification | Spec ID | Carreiro-Silva, Marina | Coral colony | ||
| 3 | Experimental treatment | Exp treat | Carreiro-Silva, Marina | |||
| 4 | Experiment duration | Exp duration | days | Carreiro-Silva, Marina | ||
| 5 | Cycles | Cycles | # | Bettencourt, Raul | Real-Time PCR, CFX Connect™ Real-Time PCR Detection System (BioRad) | Cycle threshold CT |
| 6 | Gene expression, fold change, relative | Gene expression | Bettencourt, Raul | Real-Time PCR, CFX Connect™ Real-Time PCR Detection System (BioRad) | ||
| 7 | Gene expression, fold change, relative, standard error | Gene expression std e | ± | Bettencourt, Raul | Real-Time PCR, CFX Connect™ Real-Time PCR Detection System (BioRad) |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC) * Processing Level: PANGAEA data processing level 3 (ProcLevel3)
Size:
2488 data points
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