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Monroe, Alison A; Schunter, Celia; Welch, Megan J; Munday, Philip L; Ravasi, Timothy (2021): Seawater carbonate chemistry and sequencing information of all biological samples and weight of 5 month old biological samples [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.944220

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Abstract:
Knowledge of adaptive potential is crucial to predicting the impacts of ocean acidification (OA) on marine organisms. In the spiny damselfish, Acanthochromis polyacanthus, individual variation in behavioural tolerance to elevated pCO2 has been observed and is associated with offspring gene expression patterns in the brain. However, the maternal and paternal contributions of this variation are unknown. To investigate parental influence of behavioural pCO2 tolerance, we crossed pCO2-tolerant fathers with pCO2-sensitive mothers and vice versa, reared their offspring at control and elevated pCO2 levels, and compared the juveniles' brain transcriptional programme. We identified a large influence of parental phenotype on expression patterns of offspring, irrespective of environmental conditions. Circadian rhythm genes, associated with a tolerant parental phenotype, were uniquely expressed in tolerant mother offspring, while tolerant fathers had a greater role in expression of genes associated with histone binding. Expression changes in genes associated with neural plasticity were identified in both offspring types: the maternal line had a greater effect on genes related to neuron growth while paternal influence impacted the expression of synaptic development genes. Our results confirm cellular mechanisms involved in responses to varying lengths of OA exposure, while highlighting the parental phenotype's influence on offspring molecular phenotype.
Keyword(s):
Acanthochromis polyacanthus; Animalia; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gene expression (incl. proteomics); Growth/Morphology; Laboratory experiment; Nekton; Pelagos; Single species; South Pacific; Tropical
Supplement to:
Monroe, Alison A; Schunter, Celia; Welch, Megan J; Munday, Philip L; Ravasi, Timothy (2021): Molecular basis of parental contributions to the behavioural tolerance of elevated pCO2 in a coral reef fish. Proceedings of the Royal Society B-Biological Sciences, 288(1964), https://doi.org/10.1098/rspb.2021.1931
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html
Ravasi, Timothy: Differentially expressed genes between all conditions compared to control, within parental pairs. rspb20211931_si_002.xlsx
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-05-13.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeRavasi, Timothystudy
2Species, unique identificationSpecies UIDRavasi, Timothy
3Species, unique identification (URI)Species UID (URI)Ravasi, Timothy
4Species, unique identification (Semantic URI)Species UID (Semantic URI)Ravasi, Timothy
5Sample IDSample IDRavasi, Timothy
6IdentificationIDRavasi, TimothyParental
7TypeTypeRavasi, TimothyParental phenotype
8TreatmentTreatRavasi, TimothyParental condition
9TreatmentTreatRavasi, TimothyOffspring condition
10ReadsReads#Ravasi, Timothyraw
11ReadsReads#Ravasi, Timothyafter quality checks
12MassMassgRavasi, Timothyoffspring
13pH, NBS scalepH NBSRavasi, TimothyPotentiometricNBS scale
14pH, standard deviationpH std dev±Ravasi, TimothyPotentiometricNBS scale
15Temperature, waterTemp°CRavasi, Timothy
16Temperature, water, standard deviationTemp std dev±Ravasi, Timothy
17SalinitySalRavasi, Timothy
18Salinity, standard deviationSal std dev±Ravasi, Timothy
19Alkalinity, totalATµmol/kgRavasi, TimothyPotentiometric titration
20Alkalinity, total, standard deviationAT std dev±Ravasi, TimothyPotentiometric titration
21Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmRavasi, TimothyCalculated using CO2SYS
22Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Ravasi, TimothyCalculated using CO2SYS
23Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
24pH, total scalepHTYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
25Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
26Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
28Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
4772 data points

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