Citation:

PANGAEA.943573

Name: Acidification and iron limitation effects on the photophysiology, growth, carbon production, and cellular pigment and trace metal quotas of the Antarctic phytoplankton Geminigera cryophila and Pseudo‐nitzschia subcurvata
Published: 2022-04-28
License: https://creativecommons.org/licenses/by/4.0/
Keywords: cryptophytes; culture experiment; diatoms; Iron limitation; Laboratory experiment; Ocean acidification; Southern Ocean

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Published: 2022-04-28 • DOI registered: 2022-05-28

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Abstract:

Ecophysiological studies looking at the combined effects of ocean acidification (OA) and iron (Fe) availability on Southern Ocean (SO) phytoplankton are still limited. To gain a better mechanistic understanding of how the two ecologically important SO phytoplankton groups cope with OA and Fe limitation, we conducted laboratory incubation experiments on the Antarctic cryptophyte Geminigera cryophila and the diatom Pseudo‐nitzschia subcurvata. Geminigera cryophila (CCMP 2564) was isolated from the Southern Ocean and obtained from Matt Johnson's Laboratory of Protistan Ecology at the Woods Hole Oceanography Institute, United States. Pseudo-nitzschia subcurvata was isolated from the Southern Ocean by P. Assmy during Polarstern expedition ANT- XXI/4. Both species were grown at 2°C under different pCO2 (400 vs. 900 μatm) and Fe (0.6 vs. 1.2 nM) conditions. For P. subcurvata, an additional high pCO2 level was applied (1400 μatm). For both species, growth, photophysiology, cellular quotas of particulate organic carbon, trace metals and pigments were assessed. Our study reveals that Fe limitation was detrimental for the growth of G. cryophila and suppressed the positive OA effect. The diatom was efficient in coping with low Fe, but was stressed by OA while both factors together strongly impacted its growth. The distinct physiological response of both species to OA and Fe limitation explains their occurrence in the field. Based on our results, Fe availability is an important modulator of OA effects on SO phytoplankton, with different implications on the occurrence of cryptophytes and diatoms in the future.

Keyword(s):

cryptophytes; culture experiment; diatoms; Iron limitation; Laboratory experiment; Ocean acidification; Southern Ocean

Supplement to:

Camoying, Marianne; Thoms, Silke; Geuer, Jana K; Koch, Boris P; Bischof, Kai; Trimborn, Scarlett (2022): In contrast to diatoms, cryptophytes are susceptible to iron limitation, but not to ocean acidification. Physiologia Plantarum, 174(1), e13614, https://doi.org/10.1111/ppl.13614

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Camoying, Marianne
2	Species	Species		Camoying, Marianne
3	Registration number of species	Reg spec no		Camoying, Marianne
4	Uniform resource locator/link to reference	URL ref		Camoying, Marianne		WoRMS AphiaID
5	Treatment: dissolved iron	T:Fe diss	nmol/l	Camoying, Marianne	Inductively coupled plasma mass spectrometer (ICP-MS), Attom, Nu Instruments
6	Treatment: partial pressure of carbon dioxide	T:pCO2	µatm	Camoying, Marianne
7	Phytoplankton growth rate	Phytopl µ	1/day	Camoying, Marianne	Light microscopy (Utermöhl 1958)
8	Growth rate, standard deviation	µ std dev	±	Camoying, Marianne	Light microscopy (Utermöhl 1958)
9	Carbon, organic, particulate	POC	pg/µm³	Camoying, Marianne	Elemental analyzer, HEKAtechGmbH, Euro EA	cell volume normalized
10	Carbon, organic, particulate, standard deviation	POC std dev	±	Camoying, Marianne	Elemental analyzer, HEKAtechGmbH, Euro EA	cell volume normalized
11	Carbon, organic, particulate, production	POC prod	pg/µm³/day	Camoying, Marianne	Elemental analyzer, HEKAtechGmbH, Euro EA	cell volume normalized
12	Carbon, organic, particulate, production, standard deviation	POC prod std dev	±	Camoying, Marianne	Elemental analyzer, HEKAtechGmbH, Euro EA	cell volume normalized
13	Nitrogen, organic, particulate	PON	pg/µm³	Camoying, Marianne	Elemental analyzer, HEKAtechGmbH, Euro EA	cell volume normalized
14	Nitrogen, organic, particulate, production, standard deviation	PON prod std dev	±	Camoying, Marianne	Elemental analyzer, HEKAtechGmbH, Euro EA	cell volume normalized
15	Carbon/Nitrogen ratio	C/N	mol/mol	Camoying, Marianne	Elemental analyzer, HEKAtechGmbH, Euro EA
16	Carbon/Nitrogen ratio, standard deviation	C/N std dev	±	Camoying, Marianne	Elemental analyzer, HEKAtechGmbH, Euro EA
17	Iron, cellular quota	QFe	amol/#	Camoying, Marianne	Inductively coupled plasma mass spectrometer (ICP-MS), Attom, Nu Instruments
18	Iron, cellular quota, standard deviation	QFe std dev	±	Camoying, Marianne	Inductively coupled plasma mass spectrometer (ICP-MS), Attom, Nu Instruments
19	Iron/Carbon ratio	Fe/C	µmol/mol	Camoying, Marianne	Inductively coupled plasma mass spectrometer (ICP-MS), Attom, Nu Instruments
20	Iron/Carbon ratio, standard deviation	Fe/C std dev	±	Camoying, Marianne	Inductively coupled plasma mass spectrometer (ICP-MS), Attom, Nu Instruments
21	Manganese/Carbon ratio	Mn/C	µmol/mol	Camoying, Marianne	Inductively coupled plasma mass spectrometer (ICP-MS), Attom, Nu Instruments
22	Manganese/Carbon ratio, standard deviation	Mn/C std dev	±	Camoying, Marianne	Inductively coupled plasma mass spectrometer (ICP-MS), Attom, Nu Instruments
23	Cobalt/Carbon ratio	Co/C	µmol/mol	Camoying, Marianne	Inductively coupled plasma mass spectrometer (ICP-MS), Attom, Nu Instruments
24	Cobalt/Carbon ratio, standard deviation	Co/C std dev	±	Camoying, Marianne	Inductively coupled plasma mass spectrometer (ICP-MS), Attom, Nu Instruments
25	Copper/Carbon ratio	Cu/C	µmol/mol	Camoying, Marianne	Inductively coupled plasma mass spectrometer (ICP-MS), Attom, Nu Instruments
26	Copper/Carbon ratio, standard deviation	Cu/C std dev	±	Camoying, Marianne	Inductively coupled plasma mass spectrometer (ICP-MS), Attom, Nu Instruments
27	Zinc/Carbon ratio	Zn/C	µmol/mol	Camoying, Marianne	Inductively coupled plasma mass spectrometer (ICP-MS), Attom, Nu Instruments
28	Zinc/Carbon ratio, standard deviation	Zn/C std dev	±	Camoying, Marianne	Inductively coupled plasma mass spectrometer (ICP-MS), Attom, Nu Instruments
29	Chlorophyll a	Chl a	fg/µm³	Camoying, Marianne	Reverse phase HPLC (High Performance Liquid Chromatography)
30	Chlorophyll a, standard deviation	Chl a std dev	±	Camoying, Marianne	Reverse phase HPLC (High Performance Liquid Chromatography)
31	Chlorophyll c2	Chl c2	fg/µm³	Camoying, Marianne	Reverse phase HPLC (High Performance Liquid Chromatography)
32	Chlorophyll c2, standard deviation	Chl c2 std dev	±	Camoying, Marianne	Reverse phase HPLC (High Performance Liquid Chromatography)
33	Fucoxanthin	Fucoxanthin	fg/µm³	Camoying, Marianne	Reverse phase HPLC (High Performance Liquid Chromatography)
34	Fucoxanthin, standard deviation	Fuco std dev	±	Camoying, Marianne	Reverse phase HPLC (High Performance Liquid Chromatography)
35	Diadinoxanthin	Diadinoxanthin	fg/µm³	Camoying, Marianne	Reverse phase HPLC (High Performance Liquid Chromatography)
36	Diadinoxanthin, standard deviation	Diadinoxanthin std dev	±	Camoying, Marianne	Reverse phase HPLC (High Performance Liquid Chromatography)
37	Alloxanthin	Alloxanthin	fg/µm³	Camoying, Marianne	Reverse phase HPLC (High Performance Liquid Chromatography)
38	Alloxanthin, standard deviation	Alloxanthin std dev	±	Camoying, Marianne	Reverse phase HPLC (High Performance Liquid Chromatography)
39	Maximum photochemical quantum yield of photosystem II	Fv/Fm		Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
40	Maximum photochemical quantum yield of photosystem II, standard deviation	Fv/Fm std dev	±	Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
41	Maximal electron transport rate, standard deviation	ETR max std dev	±	Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
42	Maximal electron transport rate, standard deviation	ETR max std dev	±	Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
43	Light saturation point	Ik	µmol/m²/s	Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
44	Light saturation point, standard deviation	Ik std dev	±	Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
45	Light use efficiency	alpha		Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)	maximum
46	Maximum light utilization efficiency, standard deviation	Alpha std dev	±	Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
47	Functional absorption cross sections of photosystem II reaction centers	Sigma PSII	nm²/PSII	Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
48	Functional absorption cross sections of photosystem II reaction centers, standard deviation	Sigma PSII std dev	±	Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
49	Functional photosystem II reaction centers	RCII	zmol/µm³	Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
50	Functional photosystem II reaction centers, standard deviation	RCII std dev	±	Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
51	Connectivity between photosystem II	p		Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
52	Connectivity between photosystem II, standard deviation	p std dev	±	Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
53	Maximum photochemical quantum yield of photosystem II, recovery	Fv/Fm recovery	%	Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)	of initial
54	Maximum photochemical quantum yield of photosystem II, recovery, standard deviation	Fv/Fm recovery std dev	±	Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
55	Irradiance	E	µmol photons/m²/s	Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
56	Electron transport rate, absolute	aETR	e/PSII/s	Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
57	Electron transport rate, absolute, standard deviation	aETR std dev	±	Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
58	Non photochemical quenching	NPQ		Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)
59	Non photochemical quenching, standard deviation	NPQ std dev	±	Camoying, Marianne	Fluorometer, fast repetition rate (FRRF)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3068 data points

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