Gómez-Letona, Markel; Arístegui, Javier; Hernández-Hernández, Nauzet; Pérez-Lorenzo, Maria; Alvarez-Salgado, Xose Anton; Teira, Eva; Sebastian, Marta (2022): Integrated abundance, cell volume, viability and leucine incorporation rates of prokaryotes in the epipelagic, mesopelagic and bathypelagic layers of the tropical and subtropical Atlantic [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.943416, In: Gómez-Letona, M et al. (2022): Abundance, cell volume, biomass, viability and leucine incorporation rates of prokaryotes from the MAFIA cruise to the tropical and subtropical Atlantic in 2015 [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.943417
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Abstract:
This dataset contains the results of the characterisation of the prokaryotic community by flow cytometry and tritiated leucine incorporation from the MAFIA cruise (Migrants and Active Flux In the Atlantic ocean). Samples were collected in the tropical and subtropical Atlantic during the MAFIA cruise (April 2015) on board the BIO Hespérides. Seawater samples were collected at 13 stations (from the Brazilian coast to the Canary Islands), from the surface down to 3500 m, using a General Oceanics oceanographic rosette equipped with 24 l PVC Niskin bottles. Abundance and cell characteristics (high nucleic acid content fraction, cell volume, viability) were based on measurements performed with a FACSCalibur (Becton-Dickinson) flow cytometer. Leucine incorporation rates were estimated with tritiated leucine (Kirchman et al. 1985) using centrifugation and filtration methods (Smith and Azam 1992). Integrated or average values of variables were estimated for the epipelagic (0-200 m), mesopelagic (200-1000 m) and bathypelagic (1000-3000 m) layers, based on an interpolated grid estimated with DIVA. The aim of this dataset was to estimate the influence of surface productivity on the standing stock, characteristics and activity (as leucine incorporation) of prokaryotes across the water column.
Keyword(s):
Supplement to:
Gómez-Letona, Markel; Arístegui, Javier; Hernández-Hernández, Nauzet; Pérez-Lorenzo, Maria; Alvarez-Salgado, Xose Anton; Teira, Eva; Sebastian, Marta (2022): Surface productivity gradients govern changes in the viability of deep ocean prokaryotes across the tropical and subtropical Atlantic. Limnology and Oceanography, lno.12248, https://doi.org/10.1002/lno.12248
Gómez-Letona, Markel; Arístegui, Javier; Hernández-Hernández, Nauzet; Pérez-Lorenzo, Maria; Álvarez‐Salgado, Xosé Antón; Teira, Eva; Sebastián, M A (preprint): Surface productivity gradients govern changes in abundance and physiological status of deep ocean prokaryotes across the tropical and subtropical Atlantic. bioRxiv, 2022.06.20.496789, https://doi.org/10.1101/2022.06.20.496789
Further details:
Calvo-Díaz, Alejandra; Morán, Xosé Anxelu G (2006): Seasonal dynamics of picoplankton in shelf waters of the southern Bay of Biscay. Aquatic Microbial Ecology, 42, 159-174, https://doi.org/10.3354/ame042159
Falcioni, Tania; Papa, Stefano; Gasol, Josep M (2008): Evaluating the Flow-Cytometric Nucleic Acid Double-Staining Protocol in Realistic Situations of Planktonic Bacterial Death. Applied and Environmental Microbiology, 74(6), 1767-1779, https://doi.org/10.1128/AEM.01668-07
Hernández-León, Santiago; UTM-CSIC (2018): MAFIA Cruise, RV Hespérides [dataset]. UTM-CSIC, https://doi.org/10.20351/29HE20150403
Kirchman, David L; K'nees, E; Hodson, Robert E (1985): Leucine incorporation and its potential as a measure of protein synthesis by bacteria in natural aquatic systems. Applied and Environmental Microbiology, 49(3), 599-607, https://doi.org/10.1128/aem.49.3.599-607.1985
Lebaron, P; Servais, Pierre; Agogué, Helene; Courties, C; Joux, Fabien (2001): Does the High Nucleic Acid Content of Individual Bacterial Cells Allow Us To Discriminate between Active Cells and Inactive Cells in Aquatic Systems? Applied and Environmental Microbiology, 67(4), 1775-1782, https://doi.org/10.1128/AEM.67.4.1775-1782.2001
Norland, Svein (1993): The relationship between biomass and volume of bacteria. In: PF Kemp, BF Sherr, EB Sherr, and JJ Cole [eds.], Handbook of Methods in Aquatic Microbial Ecology, 1st edition, Lewis Publishers, 303-307
Smith, David Charles; Azam, Farooq (1992): A simple, economical method for measuring bacterial protein synthesis rates in seawater using 3H-leucine. Marine Microbial Food Webs, 6(2), 107-114
Project(s):
Biogeochemical impact of mesoscale and sub-mesoscale processes along the life history of cyclonic and anticyclonic eddies: plankton variability and productivity (e-IMPACT)
Funding:
Spanish Ministry of Science, Innovation and Universities (MICINN), grant/award no. CTM2012-39587-C04: Migrants and Active Flux In the Atlantic Ocean
Spanish Ministry of Science, Innovation and Universities (MICINN), grant/award no. CTM2015-69392-C3: Constraining organic carbon fluxes in an eastern boundary upwelling ecosystem (NW Africa): the role of non-sinking carbon in the context of the biological pump
Spanish Ministry of Science, Innovation and Universities (MICINN), grant/award no. CTM2017-83362-R: INTERES: Papel de las interacciones fitoplancton-bacterias en la respuesta del plancton microbiano a la entrada de nutrientes alóctonos
Spanish Ministry of Science, Innovation and Universities (MICINN), grant/award no. PID2019-109084RB-C21: Biogeochemical impact of mesoscale and sub-mesoscale processes along the life history of cyclonic and anticyclonic eddies: plankton variability and productivity
Coverage:
Median Latitude: 7.387423 * Median Longitude: -24.348562 * South-bound Latitude: -13.124000 * West-bound Longitude: -33.958800 * North-bound Latitude: 27.404000 * East-bound Longitude: -15.946000
Date/Time Start: 2015-04-05T00:45:00 * Date/Time End: 2015-04-28T20:32:07
Minimum DEPTH, water: 0 m * Maximum DEPTH, water: 3000 m
Event(s):
MAFIA_01_D-1 * Latitude: -13.124000 * Longitude: -33.958800 * Date/Time Start: 2015-04-05T20:48:00 * Date/Time End: 2015-04-05T00:45:00 * Location: Atlantic Ocean * Campaign: MAFIA * Basis: Hespérides * Method/Device: CTD/Rosette (CTD-RO)
MAFIA_02_L-1 * Latitude: -10.000700 * Longitude: -31.823800 * Date/Time Start: 2015-04-07T07:30:00 * Date/Time End: 2015-04-07T11:15:00 * Location: Atlantic Ocean * Campaign: MAFIA * Basis: Hespérides * Method/Device: CTD/Rosette (CTD-RO)
MAFIA_03_L-1 * Latitude: -6.576700 * Longitude: -30.139000 * Date/Time Start: 2015-04-09T05:08:00 * Date/Time End: 2015-04-09T09:10:00 * Location: Atlantic Ocean * Campaign: MAFIA * Basis: Hespérides * Method/Device: CTD/Rosette (CTD-RO)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Arístegui, Javier | |||
| 2 | Station label | Station | Arístegui, Javier | |||
| 3 | Campaign | Campaign | Arístegui, Javier | |||
| 4 | Date/Time of event | Date/Time | Arístegui, Javier | |||
| 5 | Latitude of event | Latitude | Arístegui, Javier | |||
| 6 | Longitude of event | Longitude | Arístegui, Javier | |||
| 7 | Sea surface chlorophyll a | SSChla | mg/m3 | Arístegui, Javier | Fluorometer, Seapoint, SCF | 0-20 m |
| 8 | Layer description | Layer | Arístegui, Javier | |||
| 9 | DEPTH, water | Depth water | m | Arístegui, Javier | Geocode – minimum | |
| 10 | DEPTH, water | Depth water | m | Arístegui, Javier | Geocode – maximum | |
| 11 | Prokaryotes, abundance, integrated | Prok abund int | #/m2 | Arístegui, Javier | Calculated | |
| 12 | High nucleic acid prokaryotes/low nucleic acid prokaryotes ratio | HNA/LNA prok | Arístegui, Javier | Calculated | average | |
| 13 | Prokaryotes, cell volume | Prok cell vol | µm3 | Arístegui, Javier | Calculated | average |
| 14 | Viable cell fraction | Viable cell frac | Arístegui, Javier | Calculated | average | |
| 15 | Bulk 3H-leucine incorporation rate, integrated | Bulk Leu inc int | pmol/m2/day | Arístegui, Javier | Calculated | |
| 16 | Specific 3H-leucine incorporation rate | Spec Leu inc | pmol/#/day | Arístegui, Javier | Calculated | viable |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
375 data points