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Gómez-Letona, Markel; Arístegui, Javier; Hernández-Hernández, Nauzet; Pérez-Lorenzo, Maria; Alvarez-Salgado, Xose Anton; Teira, Eva; Sebastian, Marta (2022): Integrated abundance, cell volume, viability and leucine incorporation rates of prokaryotes in the epipelagic, mesopelagic and bathypelagic layers of the tropical and subtropical Atlantic [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.943416, In: Gómez-Letona, M et al. (2022): Abundance, cell volume, biomass, viability and leucine incorporation rates of prokaryotes from the MAFIA cruise to the tropical and subtropical Atlantic in 2015 [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.943417

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Abstract:
This dataset contains the results of the characterisation of the prokaryotic community by flow cytometry and tritiated leucine incorporation from the MAFIA cruise (Migrants and Active Flux In the Atlantic ocean). Samples were collected in the tropical and subtropical Atlantic during the MAFIA cruise (April 2015) on board the BIO Hespérides. Seawater samples were collected at 13 stations (from the Brazilian coast to the Canary Islands), from the surface down to 3500 m, using a General Oceanics oceanographic rosette equipped with 24 l PVC Niskin bottles. Abundance and cell characteristics (high nucleic acid content fraction, cell volume, viability) were based on measurements performed with a FACSCalibur (Becton-Dickinson) flow cytometer. Leucine incorporation rates were estimated with tritiated leucine (Kirchman et al. 1985) using centrifugation and filtration methods (Smith and Azam 1992). Integrated or average values of variables were estimated for the epipelagic (0-200 m), mesopelagic (200-1000 m) and bathypelagic (1000-3000 m) layers, based on an interpolated grid estimated with DIVA. The aim of this dataset was to estimate the influence of surface productivity on the standing stock, characteristics and activity (as leucine incorporation) of prokaryotes across the water column.
Keyword(s):
Atlantic; bathypelagic; Biomass; cell volume; cytometry; deep ocean; leucine incorporation; mesopelagic; microbial community; NADS; productivity; prokaryote; prokaryotic abundance; viability
Supplement to:
Gómez-Letona, Markel; Arístegui, Javier; Hernández-Hernández, Nauzet; Pérez-Lorenzo, Maria; Alvarez-Salgado, Xose Anton; Teira, Eva; Sebastian, Marta (2022): Surface productivity gradients govern changes in abundance and physiological status of deep ocean prokaryotes across the tropical and subtropical Atlantic. bioRxiv, 2022.06.20.496789, https://doi.org/10.1101/2022.06.20.496789
Gómez-Letona, Markel; Arístegui, Javier; Hernández-Hernández, Nauzet; Pérez-Lorenzo, Maria; Alvarez-Salgado, Xose Anton; Teira, Eva; Sebastian, Marta (2022): Surface productivity gradients govern changes in the viability of deep ocean prokaryotes across the tropical and subtropical Atlantic. Limnology and Oceanography, lno.12248, https://doi.org/10.1002/lno.12248
Further details:
Calvo-Díaz, Alejandra; Morán, Xosé Anxelu G (2006): Seasonal dynamics of picoplankton in shelf waters of the southern Bay of Biscay. Aquatic Microbial Ecology, 42, 159-174, https://doi.org/10.3354/ame042159
Falcioni, Tania; Papa, Stefano; Gasol, Josep M (2008): Evaluating the Flow-Cytometric Nucleic Acid Double-Staining Protocol in Realistic Situations of Planktonic Bacterial Death. Applied and Environmental Microbiology, 74(6), 1767-1779, https://doi.org/10.1128/AEM.01668-07
Hernández-León, Santiago; UTM-CSIC (2018): MAFIA Cruise, RV Hespérides. UTM-CSIC, https://doi.org/10.20351/29HE20150403
Kirchman, David L; K'nees, E; Hodson, Robert E (1985): Leucine incorporation and its potential as a measure of protein synthesis by bacteria in natural aquatic systems. Applied and Environmental Microbiology, 49(3), 599-607, https://doi.org/10.1128/aem.49.3.599-607.1985
Lebaron, P; Servais, Pierre; Agogué, Helene; Courties, C; Joux, Fabien (2001): Does the High Nucleic Acid Content of Individual Bacterial Cells Allow Us To Discriminate between Active Cells and Inactive Cells in Aquatic Systems? Applied and Environmental Microbiology, 67(4), 1775-1782, https://doi.org/10.1128/AEM.67.4.1775-1782.2001
Norland, Svein (1993): The relationship between biomass and volume of bacteria. In PF Kemp, BF Sherr, EB Sherr, and JJ Cole [eds.], Handbook of Methods in Aquatic Microbial Ecology, 1st edition, Lewis Publishers, 303-307
Smith, David Charles; Azam, Farooq (1992): A simple, economical method for measuring bacterial protein synthesis rates in seawater using 3H-leucine. Marine Microbial Food Webs, 6(2), 107-114
Funding:
Horizon 2020 (H2020), grant/award no. 817806: Sustainable management of mesopelagic resources
Ministerio de Ciencia e Innovación (MICINN), grant/award no. CTM2012-39587-C04: Migrants and Active Flux In the Atlantic Ocean
Ministerio de Ciencia e Innovación (MICINN), grant/award no. CTM2015-69392-C3: Constraining organic carbon fluxes in an eastern boundary upwelling ecosystem (NW Africa): the role of non-sinking carbon in the context of the biological pump
Ministerio de Ciencia e Innovación (MICINN), grant/award no. CTM2017-83362-R: INTERES: Papel de las interacciones fitoplancton-bacterias en la respuesta del plancton microbiano a la entrada de nutrientes alóctonos
Ministerio de Ciencia e Innovación (MICINN), grant/award no. PID2019-109084RB-C21: Biogeochemical impact of mesoscale and sub-mesoscale processes along the life history of cyclonic and anticyclonic eddies: plankton variability and productivity
Coverage:
Median Latitude: 7.387423 * Median Longitude: -24.348562 * South-bound Latitude: -13.124000 * West-bound Longitude: -33.958800 * North-bound Latitude: 27.404000 * East-bound Longitude: -15.946000
Date/Time Start: 2015-04-05T00:45:00 * Date/Time End: 2015-04-28T20:32:07
Minimum DEPTH, water: m * Maximum DEPTH, water: 3000 m
Event(s):
MAFIA_01_D-1  * Latitude: -13.124000 * Longitude: -33.958800 * Date/Time Start: 2015-04-05T20:48:00 * Date/Time End: 2015-04-05T00:45:00 * Location: Atlantic Ocean * Campaign: MAFIA * Basis: Hespérides * Method/Device: CTD/Rosette (CTD-RO)
MAFIA_02_L-1  * Latitude: -10.000700 * Longitude: -31.823800 * Date/Time Start: 2015-04-07T07:30:00 * Date/Time End: 2015-04-07T11:15:00 * Location: Atlantic Ocean * Campaign: MAFIA * Basis: Hespérides * Method/Device: CTD/Rosette (CTD-RO)
MAFIA_03_L-1  * Latitude: -6.576700 * Longitude: -30.139000 * Date/Time Start: 2015-04-09T05:08:00 * Date/Time End: 2015-04-09T09:10:00 * Location: Atlantic Ocean * Campaign: MAFIA * Basis: Hespérides * Method/Device: CTD/Rosette (CTD-RO)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
Event labelEventArístegui, Javier
Station labelStationArístegui, Javier
CampaignCampaignArístegui, Javier
Date/Time of eventDate/TimeArístegui, Javier
Latitude of eventLatitudeArístegui, Javier
Longitude of eventLongitudeArístegui, Javier
Sea surface chlorophyll aSSChlamg/m3Arístegui, JavierChlorophyll fluorometer, Seapoint, Seapoint chlorophyll fluorometer0-20 m
Layer descriptionLayerArístegui, Javier
DEPTH, waterDepth watermArístegui, JavierGeocode – minimum
10 DEPTH, waterDepth watermArístegui, JavierGeocode – maximum
11 Prokaryotes, abundance, integratedProk abund int#/m2Arístegui, JavierCalculated
12 High nucleic acid prokaryotes/low nucleic acid prokaryotes ratioHNA/LNA prokArístegui, JavierCalculatedaverage
13 Prokaryotes, cell volumeProk cell volµm3Arístegui, JavierCalculatedaverage
14 Viable cell fractionViable cell fracArístegui, JavierCalculatedaverage
15 Bulk 3H-leucine incorporation rate, integratedBulk Leu inc intpmol/m2/dayArístegui, JavierCalculated
16 Specific 3H-leucine incorporation rateSpec Leu incpmol/#/dayArístegui, JavierCalculatedviable
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
375 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Event

Station

Campaign

Date/Time

Latitude

Longitude

SSChla [mg/m3]
(0-20 m, Chlorophyll fluoromet...)

Layer

Depth water [m]
(minimum)
10 
Depth water [m]
(maximum)
11 
Prok abund int [#/m2]
(Calculated)
12 
HNA/LNA prok
(average, Calculated)
13 
Prok cell vol [µm3]
(average, Calculated)
14 
Viable cell frac
(average, Calculated)
15 
Bulk Leu inc int [pmol/m2/day]
(Calculated)
16 
Spec Leu inc [pmol/#/day]
(viable, Calculated)
MAFIA_01_D-1 1MAFIA2015-04-05T20:48:00-13.1240-33.95880.033Epipelagic (0-200m)0200791111500000000.28740.03580.81899538630.00.0000001630
MAFIA_01_D-11MAFIA2015-04-05T20:48:00-13.1240-33.95880.033Mesopelagic (200-1000m)2001000488542200000000.52400.06780.56162979582.00.0000000764
MAFIA_01_D-11MAFIA2015-04-05T20:48:00-13.1240-33.95880.033Bathypelagic (1000-3000m)10003000506404000000000.62990.11940.40993387871.00.0000001531
MAFIA_02_L-1 2MAFIA2015-04-07T07:30:00-10.0007-31.82380.057Epipelagic (0-200m)0200701117000000000.33020.03380.565339763560.00.0000012922
MAFIA_02_L-12MAFIA2015-04-07T07:30:00-10.0007-31.82380.057Mesopelagic (200-1000m)2001000345745800000000.53680.06970.570519166434.00.0000004081
MAFIA_02_L-12MAFIA2015-04-07T07:30:00-10.0007-31.82380.057Bathypelagic (1000-3000m)10003000403638500000000.58360.11560.45453661716.00.0000001221
MAFIA_03_L-1 3MAFIA2015-04-09T05:08:00-6.5767-30.13900.050Epipelagic (0-200m)0200819248900000000.36310.04130.762237813400.00.0000005521
MAFIA_03_L-13MAFIA2015-04-09T05:08:00-6.5767-30.13900.050Mesopelagic (200-1000m)2001000405116900000000.51720.07220.55169839466.00.0000001937
MAFIA_03_L-13MAFIA2015-04-09T05:08:00-6.5767-30.13900.050Bathypelagic (1000-3000m)10003000444187500000000.56450.10340.54842805533.00.0000000723
MAFIA_04_L-1 4MAFIA2015-04-11T06:15:00-3.1718-28.44300.085Epipelagic (0-200m)0200821302500000000.36060.04250.818826107856.00.0000005467
MAFIA_04_L-14MAFIA2015-04-11T06:15:00-3.1718-28.44300.085Mesopelagic (200-1000m)2001000466860900000000.54660.07620.55747841409.00.0000003913
MAFIA_04_L-14MAFIA2015-04-11T06:15:00-3.1718-28.44300.085Bathypelagic (1000-3000m)10003000512688500000000.57180.11220.45152431113.50.0000000759
MAFIA_05_L-1 5MAFIA2015-04-13T06:18:000.2445-26.78270.075Epipelagic (0-200m)0200741038600000000.36990.05590.840220415867.00.0000005118
MAFIA_05_L-15MAFIA2015-04-13T06:18:000.2445-26.78270.075Mesopelagic (200-1000m)2001000630837300000000.54520.08380.62015796932.00.0000000815
MAFIA_05_L-15MAFIA2015-04-13T06:18:000.2445-26.78270.075Bathypelagic (1000-3000m)10003000563923500000000.55120.11130.59963845475.00.0000001097
MAFIA_06_L-1 6MAFIA2015-04-15T06:00:003.7190-25.21920.142Epipelagic (0-200m)02001089871800000000.37810.04600.825820794797.00.0000002024
MAFIA_06_L-16MAFIA2015-04-15T06:00:003.7190-25.21920.142Mesopelagic (200-1000m)2001000565609200000000.55260.06410.63338625093.00.0000002123
MAFIA_06_L-16MAFIA2015-04-15T06:00:003.7190-25.21920.142Bathypelagic (1000-3000m)10003000523573000000000.56660.08590.48212221892.00.0000001196
MAFIA_07_L-1 7MAFIA2015-04-17T05:08:007.3008-23.92780.127Epipelagic (0-200m)0200725870000000000.41650.05910.837825764425.00.0000003912
MAFIA_07_L-17MAFIA2015-04-17T05:08:007.3008-23.92780.127Mesopelagic (200-1000m)2001000634339400000000.56950.06670.663210227054.00.0000001894
MAFIA_07_L-17MAFIA2015-04-17T05:08:007.3008-23.92780.127Bathypelagic (1000-3000m)10003000559090000000000.63170.09100.54862123331.50.0000000889
MAFIA_08_L-1 8MAFIA2015-04-19T05:45:0010.8673-22.64500.257Epipelagic (0-200m)0200700362000000000.44150.05100.863712928592.40.0000001846
MAFIA_08_L-18MAFIA2015-04-19T05:45:0010.8673-22.64500.257Mesopelagic (200-1000m)2001000714557600000000.58560.05350.78884083134.00.0000000607
MAFIA_08_L-18MAFIA2015-04-19T05:45:0010.8673-22.64500.257Bathypelagic (1000-3000m)10003000597967000000000.63370.07590.60471650490.00.0000000596
MAFIA_09_L-1 9MAFIA2015-04-21T05:25:0014.4443-21.35470.960Epipelagic (0-200m)0200860833900000000.48650.04450.855915463753.00.0000001830
MAFIA_09_L-19MAFIA2015-04-21T05:25:0014.4443-21.35470.960Mesopelagic (200-1000m)2001000808153600000000.55490.05430.79628020821.00.0000001189
MAFIA_09_L-19MAFIA2015-04-21T05:25:0014.4443-21.35470.960Bathypelagic (1000-3000m)10003000723805500000000.57260.07610.63142276847.00.0000000683
MAFIA_10_D-1 10MAFIA2015-04-23T05:02:0018.0648-20.14781.425Epipelagic (0-200m)02001153444700000000.49620.05760.888713823975.00.0000001227
MAFIA_10_D-110MAFIA2015-04-23T05:02:0018.0648-20.14781.425Mesopelagic (200-1000m)20010001090030600000000.47800.04490.80416654172.00.0000000781
MAFIA_10_D-110MAFIA2015-04-23T05:02:0018.0648-20.14781.425Bathypelagic (1000-3000m)10003000808160000000000.58200.07320.69511856121.50.0000000466
MAFIA_11_L-1 11MAFIA2015-04-25T05:53:0021.6315-18.76005.322Epipelagic (0-200m)02001564230900000000.64230.04880.900044111418.00.0000003416
MAFIA_11_L-111MAFIA2015-04-25T05:53:0021.6315-18.76005.322Mesopelagic (200-1000m)20010001402829100000000.61390.05330.78355519609.10.0000000242
MAFIA_11_L-111MAFIA2015-04-25T05:53:0021.6315-18.76005.322Bathypelagic (1000-3000m)10003000957794000000000.66380.08110.68962481298.00.0000000428
MAFIA_12_L-1 12MAFIA2015-04-27T05:44:0025.2335-17.38350.201Epipelagic (0-200m)02001208161100000000.39800.04620.8787
MAFIA_12_L-112MAFIA2015-04-27T05:44:0025.2335-17.38350.201Mesopelagic (200-1000m)20010001305908100000000.42590.06510.8219
MAFIA_12_L-112MAFIA2015-04-27T05:44:0025.2335-17.38350.201Bathypelagic (1000-3000m)10003000752680000000000.55320.08980.7158
MAFIA_13-1 13MAFIA2015-04-28T20:32:0727.4040-15.94600.227Epipelagic (0-200m)02001173796200000000.31440.0532
MAFIA_13-113MAFIA2015-04-28T20:32:0727.4040-15.94600.227Mesopelagic (200-1000m)20010001127308100000000.45660.0784
MAFIA_13-113MAFIA2015-04-28T20:32:0727.4040-15.94600.227Bathypelagic (1000-3000m)10003000734500000000000.52660.0904