Kittu, Leila; Paul, Allanah Joy; Fernández-Méndez, Mar; Riebesell, Ulf (2021): Nitrogen and carbon fixation rates and organic matter concentrations from water column samples during RV Maria S. Merian cruise MSM80 off the northern Humboldt Upwelling System [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.939726
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Published: 2021-12-20 • DOI registered: 2022-01-31
Abstract:
This data is part of the BMBF projects CUSCO (Coastal Upwelling Systems in a Changing Ocean) and BioTip subproject Humboldt Tipping. Here we report water column nitrogen fixation, carbon fixation rates and particulate organic matter composition from the upper 300 m. Data was collected during cruise number MSM80 with research vessel Maria S. Merian from 23.12.2018 - 30.01.2019 (from Panama to Valparaiso) in the Humboldt Upwelling system off the Eastern Tropical south Pacific. Samples were taken by CTD- rosette sampler from different depths, injected with 15N labelled N2 gas based on the modified dissolution method (Großkopf et al., 2012 and Mohr et al., 2010) and additionally 13C labeled sodium bicarbonate. Samples were incubated for 24 hours at light intensities that resemble the in situ light conditions. After incubation a volume of the sample (20 - 1500 ml) was filtered onto a pre-combusted Whatman GF/75 filter. Filters were frozen, transported to the institute on dry ice and measured on a mass spectrometer for Delta15N and 13C (Delta V Advantage Isotope Ratio MS, ThermoFisher) with the ConFlo IV interface (ThermoFisher). Nitrogen fixation rates were calculated based on Montoya et al (1996) while carbon fixation rates were calculated based on the equation by Slawyk et al. (1977). Limits of detection (LOD) and minimum quantifiable rates (MQR) for Nitrogen fixation rates were calculated according to the criteria described by White et al. (2020) and standard error propagation methods described in Gradoville et al. (2017) respectively. POP and Biogenic silica concentrations were measured spectrophotometrically following Hansen and Koroleff (1999).
Keyword(s):
Supplement to:
Kittu, Leila; Paul, Allanah Joy; Fernández-Méndez, Mar; Hopwood, Mark James; Riebesell, Ulf (2023): Coastal N 2 Fixation Rates Coincide Spatially With Nitrogen Loss in the Humboldt Upwelling System off Peru. Global Biogeochemical Cycles, 37(2), https://doi.org/10.1029/2022GB007578
Related to:
Gradoville, Mary R; Bombar, Deniz; Crump, Byron C; Letelier, Ricardo M; Zehr, Jonathan P; White, Angelicque E (2017): Diversity and activity of nitrogen-fixing communities across ocean basins. Limnology and Oceanography, 62(5), 1895-1909, https://doi.org/10.1002/lno.10542
Großkopf, Tobias; Mohr, Wiebke; Baustian, Tina; Schunck, Harald; Gill, Diana; Kuypers, Marcel MM; Lavik, Gaute; Schmitz, Ruth A; Wallace, Douglas WR; LaRoche, Julie (2012): Doubling of marine dinitrogen-fixation rates based on direct measurements. Nature, 488, 361-364, https://doi.org/10.1038/nature11338
Hansen, Hans Peter; Koroleff, F (1999): Determination of nutrients. In: Grasshoff, Klaus; Kremling, Klaus; Ehrhardt, Manfred (eds.), Methods of Seawater Analysis, Wiley-VCH Verlag GmbH, Weinheim, Germany, 159-226, https://doi.org/10.1002/9783527613984.ch10
Mohr, Wiebke; Großkopf, Tobias; Wallace, Douglas WR; LaRoche, Julie (2010): Methodological Underestimation of Oceanic Nitrogen Fixation Rates. PLoS ONE, 5(9), e12583, https://doi.org/10.1371/journal.pone.0012583
Montoya, Joseph P; Voss, Maren; Kähler, Paul; Capone, D G (1996): A Simple, High-Precision, High-Sensitivity Tracer Assay for N2 Fixation. Applied and Environmental Microbiology, 62(3), 986-993, https://doi.org/10.1128/aem.62.3.986-993.1996
Slawyk, G; Collos, Yves; Auclair, Jean-Christian (1977): The use of the 13C and 15N isotopes for the simultaneous measurement of carbon and nitrogen turnover rates in marine phytoplankton. Limnology and Oceanography, 22(5), 925-932, https://doi.org/10.4319/lo.1977.22.5.0925
White, Angelicque E; Granger, Julie; Selden, Corday; Gradoville, Mary R; Potts, Lindsey; Bourbonnais, Annie; Fulweiler, Robinson W; Knapp, Angela N; Mohr, Wiebke; Moisander, Pia H; Tobias, Craig R; Caffin, Mathieu; Wilson, Samuel T; Benavides, Mar; Bonnet, Sophie; Mulholland, Margaret R; Chang, Bonnie X (2020): A critical review of the 15N2 tracer method to measure diazotrophic production in pelagic ecosystems. Limnology and Oceanography-Methods, 18(4), 129-147, https://doi.org/10.1002/lom3.10353
Project(s):
Sozial-ökologische Kipppunkte im Humboldt-System (HumboldtTipping)
Funding:
Federal Ministry of Education and Research (BMBF), grant/award no. 01LC1823B: HumboldtTipping Teilprojekt 2: Modellierung von mariner Biogeochemie bis Fisch (End-to-End Modellierung)
Coverage:
Median Latitude: -13.189146 * Median Longitude: -77.087612 * South-bound Latitude: -16.000000 * West-bound Longitude: -80.999000 * North-bound Latitude: -8.499000 * East-bound Longitude: -74.166000
Date/Time Start: 2018-12-27T18:52:00 * Date/Time End: 2019-01-25T01:21:00
Minimum DEPTH, water: 5 m * Maximum DEPTH, water: 300 m
Event(s):
MSM80_1-1 * Latitude: -8.499583 * Longitude: -80.999983 * Date/Time: 2018-12-27T19:19:00 * Elevation: -6233.3 m * Campaign: MSM80 (CUSCO-1) * Basis: Maria S. Merian * Method/Device: CTD/Rosette (CTD-RO)
MSM80_7-1 * Latitude: -8.499133 * Longitude: -80.031050 * Date/Time: 2018-12-28T22:15:00 * Elevation: -357.1 m * Campaign: MSM80 (CUSCO-1) * Basis: Maria S. Merian * Method/Device: CTD/Rosette (CTD-RO)
MSM80_14-1 * Latitude: -8.499667 * Longitude: -79.167017 * Date/Time: 2018-12-29T18:54:00 * Elevation: -66.6 m * Campaign: MSM80 (CUSCO-1) * Basis: Maria S. Merian * Method/Device: CTD/Rosette (CTD-RO)
Parameter(s):
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1538 data points