Citation:

PANGAEA.939726

Name: Nitrogen and carbon fixation rates and organic matter concentrations from water column samples during RV Maria S. Merian cruise MSM80 off the northern Humboldt Upwelling System
Published: 2021-12-20
License: https://creativecommons.org/licenses/by/4.0/
Keywords: carbon fixation; eastern tropical South Pacific; Humboldt Current System; nitrogen fixation; particulate organic matter composition; particulate organic matter concentration; Upwelling

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Published: 2021-12-20 • DOI registered: 2022-01-31

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Abstract:

This data is part of the BMBF projects CUSCO (Coastal Upwelling Systems in a Changing Ocean) and BioTip subproject Humboldt Tipping. Here we report water column nitrogen fixation, carbon fixation rates and particulate organic matter composition from the upper 300 m. Data was collected during cruise number MSM80 with research vessel Maria S. Merian from 23.12.2018 - 30.01.2019 (from Panama to Valparaiso) in the Humboldt Upwelling system off the Eastern Tropical south Pacific. Samples were taken by CTD- rosette sampler from different depths, injected with 15N labelled N2 gas based on the modified dissolution method (Großkopf et al., 2012 and Mohr et al., 2010) and additionally 13C labeled sodium bicarbonate. Samples were incubated for 24 hours at light intensities that resemble the in situ light conditions. After incubation a volume of the sample (20 - 1500 ml) was filtered onto a pre-combusted Whatman GF/75 filter. Filters were frozen, transported to the institute on dry ice and measured on a mass spectrometer for Delta15N and 13C (Delta V Advantage Isotope Ratio MS, ThermoFisher) with the ConFlo IV interface (ThermoFisher). Nitrogen fixation rates were calculated based on Montoya et al (1996) while carbon fixation rates were calculated based on the equation by Slawyk et al. (1977). Limits of detection (LOD) and minimum quantifiable rates (MQR) for Nitrogen fixation rates were calculated according to the criteria described by White et al. (2020) and standard error propagation methods described in Gradoville et al. (2017) respectively. POP and Biogenic silica concentrations were measured spectrophotometrically following Hansen and Koroleff (1999).

Keyword(s):

carbon fixation; eastern tropical South Pacific; Humboldt Current System; nitrogen fixation; particulate organic matter composition; particulate organic matter concentration; Upwelling

Supplement to:

Kittu, Leila; Paul, Allanah Joy; Fernández-Méndez, Mar; Hopwood, Mark James; Riebesell, Ulf (2023): Coastal N 2 Fixation Rates Coincide Spatially With Nitrogen Loss in the Humboldt Upwelling System off Peru. Global Biogeochemical Cycles, 37(2), https://doi.org/10.1029/2022GB007578

Related to:

Gradoville, Mary R; Bombar, Deniz; Crump, Byron C; Letelier, Ricardo M; Zehr, Jonathan P; White, Angelicque E (2017): Diversity and activity of nitrogen-fixing communities across ocean basins. Limnology and Oceanography, 62(5), 1895-1909, https://doi.org/10.1002/lno.10542

Großkopf, Tobias; Mohr, Wiebke; Baustian, Tina; Schunck, Harald; Gill, Diana; Kuypers, Marcel MM; Lavik, Gaute; Schmitz, Ruth A; Wallace, Douglas WR; LaRoche, Julie (2012): Doubling of marine dinitrogen-fixation rates based on direct measurements. Nature, 488, 361-364, https://doi.org/10.1038/nature11338

Hansen, Hans Peter; Koroleff, F (1999): Determination of nutrients. In: Grasshoff, Klaus; Kremling, Klaus; Ehrhardt, Manfred (eds.), Methods of Seawater Analysis, Wiley-VCH Verlag GmbH, Weinheim, Germany, 159-226, https://doi.org/10.1002/9783527613984.ch10

Mohr, Wiebke; Großkopf, Tobias; Wallace, Douglas WR; LaRoche, Julie (2010): Methodological Underestimation of Oceanic Nitrogen Fixation Rates. PLoS ONE, 5(9), e12583, https://doi.org/10.1371/journal.pone.0012583

Montoya, Joseph P; Voss, Maren; Kähler, Paul; Capone, D G (1996): A Simple, High-Precision, High-Sensitivity Tracer Assay for N2 Fixation. Applied and Environmental Microbiology, 62(3), 986-993, https://doi.org/10.1128/aem.62.3.986-993.1996

Slawyk, G; Collos, Yves; Auclair, Jean-Christian (1977): The use of the 13C and 15N isotopes for the simultaneous measurement of carbon and nitrogen turnover rates in marine phytoplankton. Limnology and Oceanography, 22(5), 925-932, https://doi.org/10.4319/lo.1977.22.5.0925

White, Angelicque E; Granger, Julie; Selden, Corday; Gradoville, Mary R; Potts, Lindsey; Bourbonnais, Annie; Fulweiler, Robinson W; Knapp, Angela N; Mohr, Wiebke; Moisander, Pia H; Tobias, Craig R; Caffin, Mathieu; Wilson, Samuel T; Benavides, Mar; Bonnet, Sophie; Mulholland, Margaret R; Chang, Bonnie X (2020): A critical review of the 15N2 tracer method to measure diazotrophic production in pelagic ecosystems. Limnology and Oceanography-Methods, 18(4), 129-147, https://doi.org/10.1002/lom3.10353

Project(s):

Coastal Upwelling System in a Changing Ocean (CUSCO)

Sozial-ökologische Kipppunkte im Humboldt-System (HumboldtTipping)

Funding:

Federal Ministry of Education and Research (BMBF), grant/award no. 01LC1823B : HumboldtTipping Teilprojekt 2: Modellierung von mariner Biogeochemie bis Fisch (End-to-End Modellierung)

Coverage:

Median Latitude: -13.189146 * Median Longitude: -77.087612 * South-bound Latitude: -16.000000 * West-bound Longitude: -80.999000 * North-bound Latitude: -8.499000 * East-bound Longitude: -74.166000

Date/Time Start: 2018-12-27T18:52:00 * Date/Time End: 2019-01-25T01:21:00

Minimum DEPTH, water: 5 m * Maximum DEPTH, water: 300 m

Event(s):

MSM80_1-1 * Latitude: -8.499583 * Longitude: -80.999983 * Date/Time: 2018-12-27T19:19:00 * Elevation: -6233.3 m * Campaign: (CUSCO-1) * Basis: * Method/Device: (CTD-RO)

MSM80_7-1 * Latitude: -8.499133 * Longitude: -80.031050 * Date/Time: 2018-12-28T22:15:00 * Elevation: -357.1 m * Campaign: (CUSCO-1) * Basis: * Method/Device: (CTD-RO)

MSM80_14-1 * Latitude: -8.499667 * Longitude: -79.167017 * Date/Time: 2018-12-29T18:54:00 * Elevation: -66.6 m * Campaign: (CUSCO-1) * Basis: * Method/Device: (CTD-RO)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		Kittu, Leila
2	Station label	Station		Kittu, Leila
3	Transect	Transect		Kittu, Leila
4	DATE/TIME	Date/Time		Kittu, Leila		Geocode
5	LATITUDE	Latitude		Kittu, Leila		Geocode
6	LONGITUDE	Longitude		Kittu, Leila		Geocode
7	Pressure, water	Press	dbar	Kittu, Leila
8	DEPTH, water	Depth water	m	Kittu, Leila		Geocode
9	Nitrogen, organic, particulate	PON	µmol/l	Kittu, Leila	Delta V Advantage Isotope Ratio Mass spectrometer with ConFlo IV interface (ThermoFisher)
10	δ15N, particulate organic nitrogen	δ15N PON	‰ air	Kittu, Leila	Delta V Advantage Isotope Ratio Mass spectrometer with ConFlo IV interface (ThermoFisher)
11	Carbon, organic, particulate	POC	µmol/l	Kittu, Leila	Delta V Advantage Isotope Ratio Mass spectrometer with ConFlo IV interface (ThermoFisher)
12	δ13C, particulate organic carbon	δ13C POC	‰ PDB	Kittu, Leila	Delta V Advantage Isotope Ratio Mass spectrometer with ConFlo IV interface (ThermoFisher)
13	Biogenic silica	bSiO2	µmol/l	Kittu, Leila	Spectrophotometry
14	Phosphorus, organic, particulate	POP	µmol/l	Kittu, Leila	Spectrophotometry
15	Nitrogen fixation rate	N2 fix	nmol/l/day	Kittu, Leila	Calculated according to Montoya et al. (1996)	average
16	Nitrogen fixation rate, standard deviation	N2 fix std dev	±	Kittu, Leila
17	Nitrogen fixation rate, limit of detection	N2 fix LOD	nmol/l/day	Kittu, Leila	after White et al. (2020)
18	Nitrogen fixation rate, minimum quantifiable rate	N2 fix MQR	nmol/l/48h	Kittu, Leila	after White et al. (2020)
19	Carbon fixation rate	C fix	mg/m³/day	Kittu, Leila	after Slawyk et al. (1977)	average
20	Carbon fixation rate, standard deviation	C fix std dev	±	Kittu, Leila

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1538 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 Station	3 Transect	4 Date/Time	5 Latitude	6 Longitude	7 Press [dbar]	8 Depth water [m]	9 PON [µmol/l]	10 δ15N PON [‰ air]	11 POC [µmol/l]	12 δ13C POC [‰ PDB]	13 bSiO2 [µmol/l]	14 POP [µmol/l]	15 N2 fix [nmol/l/day]	16 N2 fix std dev [±]	17 N2 fix LOD [nmol/l/day]	18 N2 fix MQR [nmol/l/48h]	19 C fix [mg/m³/day]	20 C fix std dev [±]
MSM80_1-1	1	Transect 1	2018-12-27T18:52	-8.499	-80.999	10.0	10	1.5981	3.137	15.9860	-24.877	0.0240	1.211	0.0000	0.0000	0.4050	0.0526	1.6652	0.3752
MSM80_1-1	1	Transect 1	2018-12-27T18:52	-8.499	-80.999	25.2	25	1.4078	2.107	11.6409	-24.846	0.0620	1.640	0.0000	0.0000	0.3644	0.0535	3.8082	1.7514
MSM80_1-1	1	Transect 1	2018-12-27T18:52	-8.499	-80.999	40.2	40	1.3976	0.513	10.9285	-25.430	0.0900	1.386	0.0000	0.0000	0.5162	0.1118	3.6278	2.0917
MSM80_1-1	1	Transect 1	2018-12-27T18:52	-8.499	-80.999	80.4	80	0.8950	6.534	7.7549	-25.729	0.0470	0.370	0.0000	0.0000	0.2891	0.1488	0.2456	0.1191
MSM80_1-1	1	Transect 1	2018-12-27T18:52	-8.499	-80.999	151.0	150	0.9885	6.528	9.3619	-25.401	0.2330	0.338	0.0000	0.0000	0.1694	0.0259	0.0000	0.0000
MSM80_1-1	1	Transect 1	2018-12-27T18:52	-8.499	-80.999	201.2	200	0.7540	6.678	7.2180	-25.050	0.1220	0.751	0.0000	0.0000	0.2230	0.1362	0.0000	0.0000
MSM80_7-1	7	Transect 1	2018-12-28T22:00	-8.499	-80.031	10.0	10	2.2578	3.577	17.2959	-23.987	0.0000	0.602	0.0000	0.0000	0.7424	0.0831	8.8829	1.2790
MSM80_7-1	7	Transect 1	2018-12-28T22:00	-8.499	-80.031	25.2	25	1.7823	3.247	13.5620	-24.681	0.0000	2.412	0.0000	0.0000	0.7601	0.2147	16.8317	1.9686
MSM80_7-1	7	Transect 1	2018-12-28T22:00	-8.499	-80.031	60.4	60	0.7579	5.050	7.1219	-25.561	0.0000	1.198	0.0000	0.0000	0.2982	0.0294	3.8471	1.0317
MSM80_7-1	7	Transect 1	2018-12-28T22:00	-8.499	-80.031	251.6	250	0.3740	6.716	6.8599	-25.036	0.1900	0.769	0.0000	0.0000	0.2099	0.1301	0.0000	0.0000
MSM80_14-1	14	Transect 1	2018-12-29T18:46	-8.499	-79.167	5.0	5	6.4730	5.476	41.6347	-18.043	2.7060	16.014	0.0000	0.0000	5.2332	3.6742	734.8545	32.0034
MSM80_14-1	14	Transect 1	2018-12-29T18:46	-8.499	-79.167	15.0	15	4.0002	5.461	26.9622	-18.412	2.1810	6.919	0.0000	0.0000	2.8114	0.3233	279.0752	48.9831
MSM80_14-1	14	Transect 1	2018-12-29T18:46	-8.499	-79.167	25.2	25	1.6273	6.117	11.7630	-19.493	1.2180	3.871	0.0000	0.0000	0.8997	0.1566	36.8659	16.6509
MSM80_14-1	14	Transect 1	2018-12-29T18:46	-8.499	-79.167	50.2	50	0.4350	5.082	9.0281	-23.928	0.8500	1.395	0.0000	0.0000	0.3687	0.0381	0.0000	0.0000
MSM80_18-1	18	Transect 2	2019-01-01T13:00	-9.499	-80.003	10.0	10	2.2197	6.289	12.9240	-22.821	0.0000	1.721	0.0000	0.0000	0.4461	0.0693	4.9155	0.3632
MSM80_18-1	18	Transect 2	2019-01-01T13:00	-9.499	-80.003	20.2	20	3.2132	7.265	16.3685	-22.814	0.0000	1.055	0.0000	0.0000	0.5775	0.1446	10.7945	2.9040
MSM80_18-1	18	Transect 2	2019-01-01T13:00	-9.499	-80.003	30.2	30	1.5901	3.861	9.2223	-23.468	0.0000	1.388	0.0000	0.0000	0.6473	0.2325	7.9842	1.5535
MSM80_18-1	18	Transect 2	2019-01-01T13:00	-9.499	-80.003	40.2	40	1.5879	4.114	8.7570	-24.098	0.0000	0.983	0.0000	0.0000	0.5733	0.0628	3.5184	0.2094
MSM80_18-1	18	Transect 2	2019-01-01T13:00	-9.499	-80.003	80.4	80	0.9932	7.120	6.3371	-23.176	0.0000	0.317	0.0000	0.0000	0.3241	0.0544	0.0000	0.0000
MSM80_18-1	18	Transect 2	2019-01-01T13:00	-9.499	-80.003	201.2	200	0.4558	7.736	4.7676	-22.661	0.0060	0.031	0.0000	0.0000	0.1863	0.0447	0.0000	0.0000
MSM80_22-1	22	Transect 2	2019-01-02T11:01	-9.499	-79.339	10.0	10	1.2456	5.513	8.8270	-23.348	0.0000	0.132	0.0000	0.0000	0.6331	0.2509	9.3682	1.1137
MSM80_22-1	22	Transect 2	2019-01-02T11:01	-9.499	-79.339	28.2	28	1.4431	5.677	8.9827	-23.226	0.0000	2.122	0.0000	0.0000	0.5634	0.0142	11.5466	3.1220
MSM80_22-1	22	Transect 2	2019-01-02T11:01	-9.499	-79.339	35.2	35	0.9206	5.507	5.5192	-23.038	0.0000	0.923	0.0000	0.0000	0.3510	0.0788	1.6461	0.8780
MSM80_22-1	22	Transect 2	2019-01-02T11:01	-9.499	-79.339	80.4	80	0.8340	7.732	5.8576	-23.660	0.0010	0.516	0.0000	0.0000	0.1605	0.0301	0.0000	0.0000
MSM80_30-1	30	Transect 2	2019-01-03T07:30	-9.499	-78.449	7.0	7	2.8687	6.432	17.8652	-19.844	3.4770	5.240	0.0000	0.0000	2.0942	0.5641	4.7162	1.4698
MSM80_30-1	30	Transect 2	2019-01-03T07:30	-9.499	-78.449	17.0	17	4.9196	6.432	36.4730	-17.551	9.9700	7.326	0.0000	0.0000	2.7633	0.1767	229.1249	38.5022
MSM80_30-1	30	Transect 2	2019-01-03T07:30	-9.499	-78.449	40.2	40	1.4700	6.382	9.9812	-21.744	2.6860	1.643	0.0000	0.0000	0.4180	0.1632	0.9888	0.2279
MSM80_30-1	30	Transect 2	2019-01-03T07:30	-9.499	-78.449	78.4	80	1.5197	7.139	9.6027	-22.397	2.0380	1.283	0.0000	0.0000	0.3175	0.0190	0.0000	0.0000
MSM80_38-1	38	Transect 3	2019-01-06T12:36	-11.996	-77.338	5.0	5	12.8753	5.557	68.5010	-19.315	6.9570	18.921	0.0000	0.0000	8.9899	0.9337	833.2930	479.3409
MSM80_38-1	38	Transect 3	2019-01-06T12:36	-11.996	-77.338	15.0	15	2.4737	7.455	12.7416	-20.626	2.9780	4.676	3.7451	3.1083	0.8201	3.0698	7.3724	0.5843
MSM80_38-1	38	Transect 3	2019-01-06T12:36	-11.996	-77.338	30.2	30	2.2123	6.840	17.8953	-24.949	1.7090	1.751	1.4106	1.6885	0.6830	1.7615	0.0000	0.0000
MSM80_38-1	38	Transect 3	2019-01-06T12:36	-11.996	-77.338	100.6	100	3.0482	8.231	15.2031	-21.924	1.9530	2.470	0.0000	0.0000	0.5709	0.2995	0.0000	0.0000
MSM80_43-1	43	Transect 3	2019-01-07T02:44	-12.000	-77.666	5.0	5	8.7070	5.385	47.2479	-18.857	1.9630	13.609	0.0000	0.0000	6.8641	0.2022	1241.9289	280.8555
MSM80_43-1	43	Transect 3	2019-01-07T02:44	-12.000	-77.666	15.0	15	2.6943	5.906	13.2454	-22.813	0.2280	1.391	0.0000	0.0000	0.9258	0.2709	44.7568	0.5146
MSM80_43-1	43	Transect 3	2019-01-07T02:44	-12.000	-77.666	30.2	30	1.5358	6.542	8.7666	-22.811	0.4450	0.791	0.0000	0.0000	0.2985	0.7918	0.6863	0.2051
MSM80_43-1	43	Transect 3	2019-01-07T02:44	-12.000	-77.666	161.0	160	0.8388	5.525	5.2481	-23.812	0.1690	0.312	0.0000	0.0000	0.2655	0.0475	0.0000	0.0000
MSM80_46-22	46_22	Transect 3	2019-01-08T18:51	-12.000	-78.000	5.0	5	5.4990	5.202	30.6466	-21.481	0.0000	6.769	9.3423	6.9480	2.5269	7.6331	142.7017	25.7978
MSM80_46-22	46_22	Transect 3	2019-01-08T18:51	-12.000	-78.000	20.2	20	3.8303	4.343	19.6358	-23.481	0.0000	2.612	3.3199	1.9501	1.5984	2.4229	67.2728	3.4182
MSM80_46-22	46_22	Transect 3	2019-01-08T18:51	-12.000	-78.000	30.2	30	1.6446	7.394	10.2461	-24.432	0.0000	0.276	0.0000	0.0000	0.2088	2.5031	0.7376	0.2324
MSM80_46-22	46_22	Transect 3	2019-01-08T18:51	-12.000	-78.000	50.2	50	1.8254	6.615	12.8670	-23.740	0.8800	0.743	0.0000	0.0000	0.3184	0.7207	0.3099	0.0674
MSM80_46-22	46_22	Transect 3	2019-01-08T18:51	-12.000	-78.000	201.2	200	1.6008	6.496	15.9502	-24.622	0.1790	0.416	0.0000	0.0000	0.1168	1.0385	0.0000	0.0000
MSM80_53-1	53	Transect 4	2019-01-11T18:31	-14.499	-77.500	5.0	5	5.6841	6.167	29.7251	-22.744	0.0000	2.565	0.0000	0.0000	1.0204	0.4587	37.5133	11.9780
MSM80_53-1	53	Transect 4	2019-01-11T18:31	-14.499	-77.500	15.0	15	6.5429	7.008	33.8456	-22.336	0.0000	5.182	1.5336	0.8683	1.3981	0.8679	101.1685	8.5938
MSM80_53-1	53	Transect 4	2019-01-11T18:31	-14.499	-77.500	30.2	30	6.3785	7.497	35.4129	-22.640	0.7390	4.388	1.9704	2.1988	0.8695	2.1741	16.0568	1.9535
MSM80_53-1	53	Transect 4	2019-01-11T18:31	-14.499	-77.500	45.2	45	3.7460	7.942	23.0442	-22.876	0.4800	2.005	1.5265	0.6174	0.8101	0.6076	1.2791	1.0847
MSM80_53-1	53	Transect 4	2019-01-11T18:31	-14.499	-77.500	75.4	75	1.6967	7.526	8.8462	-22.257	0.7260	0.603	0.0000	0.0000	0.5029	0.3365	0.0000	0.0000
MSM80_53-1	53	Transect 4	2019-01-11T18:31	-14.499	-77.500	302.0	300	1.6664	8.146	8.6298	-23.495	0.0860	0.276	0.0000	0.0000	0.2915	0.4905	0.0000	0.0000
MSM80_63-1	63	Transect 4	2019-01-13T12:55	-14.500	-76.167	5.0	5	4.1996	6.550	24.4247	-22.896	1.2410	4.949	0.0000	0.0000	1.8142	0.5496	100.1113	9.8785
MSM80_63-1	63	Transect 4	2019-01-13T12:55	-14.500	-76.167	15.0	15	9.5967	6.600	53.8291	-22.012	1.0640	10.039	0.0000	0.0000	4.9466	0.5520	135.2758	26.5527
MSM80_63-1	63	Transect 4	2019-01-13T12:55	-14.500	-76.167	30.2	30	1.3763	7.889	8.9666	-22.543	0.8960	1.259	0.0000	0.0000	0.4266	0.1541	1.0263	0.0364
MSM80_63-1	63	Transect 4	2019-01-13T12:55	-14.500	-76.167	130.8	130	1.5048	9.219	9.6751	-22.512	0.6010	1.189	1.2454	0.3481	0.3416	0.4007	0.0000	0.0000
MSM80_65-1	65	Transect 4	2019-01-13T12:55	-14.500	-76.019	5.0	5	3.2390	6.821	21.0611	-23.052	1.5560	3.593	0.0000	0.0000	1.6735	0.5588	99.0100	20.6143
MSM80_65-1	65	Transect 4	2019-01-13T12:55	-14.500	-76.019	10.0	10	4.7804	8.753	26.3618	-22.523	1.2410	6.818	0.0000	0.0000	1.7717	0.8467	27.1852	24.4734
MSM80_65-1	65	Transect 4	2019-01-13T12:55	-14.500	-76.019	30.2	30	3.3985	9.580	14.5075	-21.936	1.1920	4.294	0.0000	0.0000	0.8967	0.2672	3.8881	0.8680
MSM80_65-1	65	Transect 4	2019-01-13T12:55	-14.500	-76.019	80.4	80	1.4408	9.115	6.4068	-22.858	0.9550	1.818	0.7136	0.3653	0.3975	0.2574	0.0000	0.0000
MSM80_67-1	67	Transect 5	2019-01-14T15:22	-15.299	-75.334	5.0	5	7.2661	5.361	46.7252	-20.350	2.8950	11.672	0.0000	0.0000	4.2341	1.3853	271.4065	39.0293
MSM80_67-1	67	Transect 5	2019-01-14T15:22	-15.299	-75.334	15.0	15	2.7404	5.364	16.8884	-21.812	1.0420	2.804	0.0000	0.0000	1.3663	0.3506	65.2683	14.1775
MSM80_67-1	67	Transect 5	2019-01-14T15:22	-15.299	-75.334	25.2	25	3.4411	4.778	19.9408	-22.117	1.4080	1.603	0.8539		0.5243	0.3163	4.1214	1.0582
MSM80_67-1	67	Transect 5	2019-01-14T15:22	-15.299	-75.334	130.8	130	1.3984	8.314	9.4315	-22.573	0.9600	1.263	0.3944	0.0263	0.3163	0.0386	0.0000	0.0000
MSM80_70-1	70	Transect 4	2019-01-15T12:46	-14.850	-75.958	5.0	5	3.7524	6.307	22.2015	-22.107	2.6810	5.621	0.0000	0.0000	2.1925	0.1567	176.0347	22.3417
MSM80_70-1	70	Transect 4	2019-01-15T12:46	-14.850	-75.958	15.0	15	1.8987	6.578	11.8806	-23.213	1.0650	0.956	0.0000	0.0000	0.5251	0.1228	10.4611	0.7264
MSM80_70-1	70	Transect 4	2019-01-15T12:46	-14.850	-75.958	25.2	25	2.7696	7.986	15.6444	-22.303	0.9600	1.741	0.0000	0.0000	0.4166	0.1175	1.6578	0.3109
MSM80_70-1	70	Transect 4	2019-01-15T12:46	-14.850	-75.958	161.0	160	1.1072	6.942	6.8112	-23.623	0.2744	0.455	0.0000	0.0000	0.1723	0.0489	0.0000	0.0000
MSM80_80-1	80	Transect 5	2019-01-17T13:30	-15.299	-75.583	5.0	5	9.5861	6.144	56.7665	-21.213	0.3580	4.236	12.8612	11.5362	1.6181	12.8125	67.3111	11.2214
MSM80_80-1	80	Transect 5	2019-01-17T13:30	-15.299	-75.583	12.0	12	8.6576	5.687	48.9617	-21.660	0.9950	30.254	30.9708	9.1212	8.6512	7.2219	437.4708	105.0550
MSM80_80-1	80	Transect 5	2019-01-17T13:30	-15.299	-75.583	20.2	20	4.0226	8.249	23.7936	-22.575	1.6960	5.344	14.5768	6.0907	1.4554	5.2824	35.0926	3.2577
MSM80_80-1	80	Transect 5	2019-01-17T13:30	-15.299	-75.583	30.2	30	1.0117	7.359	7.3141	-22.752	1.0010	1.679	1.7754	0.4931	0.6009	0.4182	1.8889	0.1460
MSM80_80-1	80	Transect 5	2019-01-17T13:30	-15.299	-75.583	80.4	80	0.7967	6.727	5.5981	-23.805	0.9920	0.732	0.8270	0.3453	0.3207	0.2836	0.0000	0.0000
MSM80_80-1	80	Transect 5	2019-01-17T13:30	-15.299	-75.583	151.0	150	2.9580	6.853	16.7262	-21.575	0.3510	0.716	0.0000		0.3130	1.8916	0.0000	0.0000
MSM80_82-1	82	Transect 5	2019-01-18T19:52	-15.300	-75.417	5.0	5	4.9476	7.930	25.0375	-22.723	0.6920	4.097	0.0000	0.0000	2.4217	0.3920	166.0016	52.0744
MSM80_82-1	82	Transect 5	2019-01-18T19:52	-15.300	-75.417	15.0	15	3.0859	7.996	16.0581	-23.390	0.3580	1.834	0.0000	0.0000	0.7442	1.1552	17.6664	4.0977
MSM80_82-1	82	Transect 5	2019-01-18T19:52	-15.300	-75.417	40.2	40	1.9583	8.198	11.6317	-22.960	0.1950	1.233	0.7387		0.3639	0.1957	6.2836	1.3395
MSM80_82-1	82	Transect 5	2019-01-18T19:52	-15.300	-75.417	151.0	150	3.7035	7.704	22.6532	19.194	0.1690	0.848	0.0000	0.0000	0.2774	0.1344	0.0000	0.0000
MSM80_89-1	89	Transect 5	2019-01-21T00:11	-15.270	-76.095	5.0	5	2.9695	6.313	17.9257	-22.599	0.3030	0.871	0.0000	0.0000	2.7650	1.4545	264.8169	22.2642
MSM80_89-1	89	Transect 5	2019-01-21T00:11	-15.270	-76.095	15.0	15	3.2247	6.105	17.2047	-22.350	1.2210	4.009	2.4698	0.2250	0.4516	0.2578	5.1201	0.1839
MSM80_90-1	90	Transect 5	2019-01-21T08:37	-15.266	-76.268	6.0	6	14.6895	6.563	82.5453	-19.835	0.0250	3.725	0.0000	0.0000	7.0185	1.1315	1034.3374	31.2205
MSM80_90-1	90	Transect 5	2019-01-21T08:37	-15.266	-76.268	12.0	12	16.2128	6.325	94.7458	-20.109	3.3070	21.163	0.0000	0.0000	6.2928	2.2971	497.9017	70.1961
MSM80_91-1	91	Transect 5	2019-01-21T15:55	-15.530	-76.267	10.0	10	29.8148	6.451	185.9074	-20.740	3.1920	17.475	0.0000	0.0000	11.6830	4.9519	879.9664	103.1263
MSM80_91-1	91	Transect 5	2019-01-21T15:55	-15.530	-76.267	20.2	20	3.5467	6.002	20.1149	-23.373	0.6870	34.468	0.0000	0.0000	0.7787	0.4221	23.7306	2.3455
MSM80_92-1	92	Transect 5	2019-01-21T18:49	-15.479	-76.267	5.0	5	91.2796	6.681	672.6513	-19.446	0.7200	6.251	0.0000	0.0000	60.2699	7.9507	1134.8663	522.2567
MSM80_92-1	92	Transect 5	2019-01-21T18:49	-15.479	-76.267	15.0	15	3.6392	4.332	23.4877	-24.292	0.0500	210.165	0.0000	0.0000	0.5686	0.1928	12.4645	1.2014
MSM80_93-1	93	Transect 5	2019-01-21T21:41	-15.428	-76.266	5.0	5	4.8310	4.184	30.6172	-21.982	0.3280	2.818	0.0000	0.0000	2.4593		267.7118	10.3917
MSM80_93-1	93	Transect 5	2019-01-21T21:41	-15.428	-76.266	30.2	30	2.3324	6.878	13.8499	-23.863	0.7200	5.475	0.0000	0.0000	0.2987	0.9322	2.9838	0.1619
MSM80_94-6	94_6	Transect 6	2019-01-22T14:00	-16.000	-75.000	10.0	10	3.5487	6.893	18.9427	-23.813	0.8500	1.512	1.3798	0.3667	1.1812	0.4947	35.9880	2.6005
MSM80_94-6	94_6	Transect 6	2019-01-22T14:00	-16.000	-75.000	20.2	20	4.5309	6.412	23.3943	-24.293	0.0000	4.452	2.2220	1.1918	0.9527	1.2445	27.4337	6.2276
MSM80_94-6	94_6	Transect 6	2019-01-22T14:00	-16.000	-75.000	30.2	30	2.4052	5.959	10.8953	-23.341	0.0000	2.904	2.3724	0.8380	0.7448	1.0164	6.5064	1.2552
MSM80_94-6	94_6	Transect 6	2019-01-22T14:00	-16.000	-75.000	100.6	100	1.1197	8.219	6.3662	-23.369	0.0000	2.430	0.4503		0.2559	0.0947	0.0000	0.0000
MSM80_95-1	95	Transect 6	2019-01-22T19:29	-16.000	-74.166	5.0	5	25.0656	7.414	177.5976	-19.498	0.4150	0.685	10.4891	5.4923	3.6800	4.7394	580.1480	233.6769
MSM80_95-1	95	Transect 6	2019-01-22T19:29	-16.000	-74.166	10.0	10	33.1300	7.342	228.8756	-19.679	2.7790	16.801	0.0000	0.0000	9.3404	3.1759	1090.9106	399.4143
MSM80_95-1	95	Transect 6	2019-01-22T19:29	-16.000	-74.166	30.2	30	1.5842	8.210	11.1283	-23.155	3.3390	38.602	1.3238	0.3815	0.3906	0.3413	2.0918	0.3417
MSM80_95-1	95	Transect 6	2019-01-22T19:29	-16.000	-74.166	80.4	80	1.1167	7.438	7.0940	-24.081	2.7790	2.011	0.8791	0.0499	0.2630	0.0998	0.0000	0.0000
MSM80_96-1	96	Transect 6	2019-01-23T18:11	-15.999	-76.000	5.0	5	3.4444	6.120	21.4511	-22.587	0.4201	0.965	2.6976	2.2892	1.4271	2.1421	55.8888	14.7806
MSM80_96-1	96	Transect 6	2019-01-23T18:11	-15.999	-76.000	17.2	17	3.9083	6.075	21.6567	-24.037	0.0970	4.719	2.0039	0.8532	1.3614	0.9611	81.9508	20.5681
MSM80_96-1	96	Transect 6	2019-01-23T18:11	-15.999	-76.000	40.2	40	1.3551	6.963	7.7337	-23.938	0.0100	3.631	0.0000	0.0000	0.6331	0.1173	43.1536	4.7470
MSM80_96-1	96	Transect 6	2019-01-23T18:11	-15.999	-76.000	100.6	100	0.8694	6.355	6.4769	-24.103	0.0320	1.485	0.7330		0.2571	0.1142	0.0000	0.0000
MSM80_99-1	99	Transect 6	2019-01-24T04:23	-16.000	-75.251	5.0	5	4.7347	8.104	27.4860	-22.746	0.1650	0.941	2.0279	1.2665	1.0715	1.5039	61.6290	3.2089
MSM80_99-1	99	Transect 6	2019-01-24T04:23	-16.000	-75.251	10.0	10	4.7772	8.074	24.7115	-23.056	0.0060	3.240	0.0000	0.0000	1.5112	0.4215	81.0604	10.0425
MSM80_99-1	99	Transect 6	2019-01-24T04:23	-16.000	-75.251	60.4	60	2.2379	7.866	12.3659	-24.404	0.4180	5.163	0.5796	0.0396	0.0396	0.0613	2.2783	0.4159
MSM80_99-1	99	Transect 6	2019-01-24T04:23	-16.000	-75.251	100.6	100	1.4203	7.624	7.8681	-23.367	0.3300	1.343	0.3771		0.2898	0.0790	0.0000	0.0000
MSM80_102-8	102_8	Transect 6	2019-01-25T01:21	-16.000	-74.495	5.0	5	4.8554	8.142	33.7451	-22.908	0.3250	1.012	2.1407	0.9852	2.0337	0.8574	152.0177	42.2231
MSM80_102-8	102_8	Transect 6	2019-01-25T01:21	-16.000	-74.495	15.0	15	3.1678	7.703	18.8840	-24.940	0.7100	6.894	0.5342	0.2923	1.0107	0.3052	36.9267	12.5978
MSM80_102-8	102_8	Transect 6	2019-01-25T01:21	-16.000	-74.495	35.2	35	2.3006	9.004	14.3406	-22.292	0.1670	2.733	1.7396	0.2719	1.0049	0.2169	13.0594	0.2847
MSM80_102-8	102_8	Transect 6	2019-01-25T01:21	-16.000	-74.495	100.6	100	1.4606	8.592	9.1032	-22.656	0.3600	3.565	0.0000	0.0000	0.2946	0.0359	0.0000	0.0000