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Müller, Carolina; Hennig, Julian; Riedel, Frank; Helle, Gerhard (2021): Impact of chemicals on stable carbon and oxygen isotope values of raw pollen [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.931663

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Published: 2021-05-18DOI registered: 2021-07-31

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Abstract:
Purification protocols to extract pollen from lake sediments contain chemicals, which alter the carbon and oxygen pollen-isotope values according to pollen characteristics and family affiliation. Modern (raw) pollen of broad-leaved (Alnus glutinosa, Betula pendula, Carpinus betulus, Corylus avellana, Fagus sylvatica and Quercus robur) and coniferous tree species (Picea abies and Pinus sylvestris) were treated with potassium hydroxide (KOH), hydrofluoric acid (HF), sodium hypochlorite (NaClO) and sulphuric acid (H2SO4) to test the impact on δ13Cpollen and δ18Opollen and assess the applicability in purification protocols. Alterations of δ13Cpollen values vary between +1.0‰ (B. pendula, NaClO-treatment) and -5.0‰ (P. sylvestris, H2SO4-treatment). The impact of chemicals on δ18Opollen are more diverse and offsets range between +1.1‰ (C. avellana, NaClO-treatment) and -17.9‰ (P. sylvestris, H2SO4-treatment). Pollen of broad-leaved and coniferous trees reacted differently to chemicals, but response patterns are generally alike. The usage of isotope-altering chemicals in purification protocols should be brought to a minimum, but the application of KOH and NaClO seems unproblematic.
Keyword(s):
chemical treatment; Pollen; pollen purification protocol; Stable carbon and oxygen isotopes
Related to:
Müller, Carolina; Hennig, Julian; Riedel, Frank; Helle, Gerhard (2021): Quantifying the impact of chemicals on stable carbon and oxygen isotope values of raw pollen. Journal of Quaternary Science, 36(3), 441-449, https://doi.org/10.1002/jqs.3300
Coverage:
Median Latitude: 49.829178 * Median Longitude: 6.089197 * South-bound Latitude: 49.738320 * West-bound Longitude: 4.756920 * North-bound Latitude: 50.063280 * East-bound Longitude: 10.533180
Minimum ELEVATION: 242 m a.s.l. * Maximum ELEVATION: 462 m a.s.l.
Event(s):
pollen_experiment_exctraction * Latitude Start: 49.738320 * Longitude Start: 4.756920 * Latitude End: 50.063280 * Longitude End: 10.533180 * Method/Device: Biological sample (BIOS)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesMüller, Carolina
2Sample IDSample IDMüller, Carolina
3LATITUDELatitudeMüller, CarolinaGeocode
4LONGITUDELongitudeMüller, CarolinaGeocode
5ELEVATIONElevationm a.s.l.Müller, CarolinaGeocode – Altitude
6TreatmentTreatMüller, Carolina
7ReplicatesRepl#Müller, Carolina
8δ13C, pollenδ13C pollenMüller, Carolina
9δ18O, pollenδ18O pollenMüller, Carolina
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2208 data points

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