Hornick, Thomas; Mach, Elke; Grossart, Hans-Peter (2021): TemBi 2014 mesocosm study: Summer storm impact on size-fractionated bacterial protein production and abundance of heterotrophic prokaryotes and picocyanobacteria [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.930921
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Published: 2021-04-29 • DOI registered: 2021-10-29
Abstract:
We simulated an experimental summer storm in large-volume (~1200 m³, ~16m depth) enclosures in Lake Stechlin (https://www.lake-lab.de) by mixing deeper water masses from the meta- and hypolimnion into the mixed layer (epilimnion). The mixing included the disturbance of a deep chlorophyll maximum (DCM) which was present at the same time of the experiment in Lake Stechlin and situated in the metalimnion of each enclosure during filling. Size-fractionated Bacterial Protein Production (BPP) of particle associated (PA, >3.0 µm) and free-living bacteria (FL, 0.2-3.0 µm) (14C-Leu incorporation) as well as abundances of PA (microscopy of DAPI stained cells on 3.0 µm polycarbonate filters) and FL heterotrophic prokaryotes and picocyanobacteria (flow cytometry of SYBR green I stained cells) were monitored for 42 days after the experimental disturbance event. Mixing increased bacterial abundance and production about 3 weeks after mixing, which was associated to a mixing-induced stimulation of phytoplankton growth in the mixed enclosures compared to the controls. Simultaneously, decreased abundances of picocyanobacteria could be observed in mixed enclosures.
Keyword(s):
Supplement to:
Grossart, Hans-Peter; Hornick, Thomas; Berger, Stella A; Nejstgaard, Jens Christian; Monaghan, Michael T; Miki, Takashi; Giling, Darren P; Selmeczy, Géza B; Sareyka, Jörg; Padisák, Judit; Kasprzak, Peter; Wurzbacher, Christian; Kirillin, Georgiy; Engelhardt, Christof; Gessner, Mark O (2025): Extreme summer storm elicits shifts in biogeochemistry, primary productivity, and plankton community structure in a large-scale lake enclosure experiment. Limnology and Oceanography Letters, lol2.70058, https://doi.org/10.1002/lol2.70058
Project(s):
Funding:
Federal Ministry of Education and Research (BMBF), grant/award no. 01LC1501G: Bridging in Biodiversity Science
Federal Ministry of Education and Research (BMBF), grant/award no. 033L041B: Stickstofflimitation in Binnengewässern
German Research Foundation (DFG), grant/award no. 233512011: An Experimental Platform for Climate Change Impact Research on Lakes
Leibniz Association (Leibniz Association), grant/award no. SAW-2011-IGB-2: Climate driven Changes in Biodiversity of Microbiota
Coverage:
Latitude: 53.143300 * Longitude: 13.028100
Date/Time Start: 2014-08-04T00:00:00 * Date/Time End: 2014-09-16T00:00:00
Minimum DEPTH, water: 2.0 m * Maximum DEPTH, water: 16.0 m
Event(s):
Comment:
Empty cells = NA
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | DATE/TIME | Date/Time | Grossart, Hans-Peter | Geocode | ||
| 2 | Day of experiment | DOE | day | Grossart, Hans-Peter | ||
| 3 | Day of the year | DOY | day | Grossart, Hans-Peter | ||
| 4 | Description | Description | Grossart, Hans-Peter | Water layer | ||
| 5 | Mesocosm label | Meso label | Grossart, Hans-Peter | LakeLab mesocosm number | ||
| 6 | Treatment | Treat | Grossart, Hans-Peter | |||
| 7 | DEPTH, water | Depth water | m | Grossart, Hans-Peter | Geocode | |
| 8 | Protein production, free-living bacteria | FL BPP-C | µg/l/day | Grossart, Hans-Peter | 14C-leucine incorporation | 14-C Leucine uptake of size fractionated water sample (0.2-3.0 µm) on Polycarbonate-filters |
| 9 | Protein production, particle associated bacteria | PA BPP-C | µg/l/day | Grossart, Hans-Peter | 14C-leucine incorporation | 14-C Leucine uptake of size fractionated water sample (>3.0 µm) on Polycarbonate-filters |
| 10 | Bacterial abundance of LDNA-Bacteria | Bact abund LDNA | # | Grossart, Hans-Peter | Flow cytometry | |
| 11 | Bacterial abundance of HDNA-Bacteria | Bact abund HDNA | # | Grossart, Hans-Peter | Flow cytometry | |
| 12 | Bacteria, free-living, abundance | Bact abund FL | # | Grossart, Hans-Peter | Flow cytometry | Derived from sum of # Bacteria_LDNA and # Bacteria_HDNA |
| 13 | Particle-associated bacterial abundance | Bact abund PA | # | Grossart, Hans-Peter | Epifluorescence microscopy after DAPI staining | Microscopy of DAPI-stained cells on Polycarbonate-filters |
| 14 | Picocyanobacteria abundance | Pikocyanobact abund | # | Grossart, Hans-Peter | Flow cytometry | |
| 15 | Nostocales cell abundance | Nostocales cell abund | # | Grossart, Hans-Peter | Epifluorescence microscopy after DAPI staining | Microscopy of DAPI-stained cells on Polycarbonate-filters |
| 16 | Nostocales biovolume | Nostocales biov | µm3/l | Grossart, Hans-Peter | derived | Derived from # Nostocales by conversion with mesocosm-specific Nostocales cell-volume |
Change history:
2025-08-22T12:47:26 – Parameter #3 was changed on request of the author to 'Day of the year' (DOY).
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2003 data points
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