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Hornick, Thomas; Mach, Elke; Grossart, Hans-Peter (2021): TemBi 2014 mesocosm study: Summer storm impact on size-fractionated bacterial protein production and abundance of heterotrophic prokaryotes and picocyanobacteria. PANGAEA, https://doi.org/10.1594/PANGAEA.930921, In: Grossart, Hans-Peter; Hornick, Thomas; Berger, Stella A; Nejstgaard, Jens Christian; Monaghan, Michael T; Miki, Takashi; Giling, Darren P; Selmeczy, Géza B; Padisák, Judit; Kasprzak, Peter; Wurzbacher, Christian; Kirillin, Georgiy; Engelhardt, Christof; Gessner, Mark O (2021): TemBi 2014 mesocosm study - Impact of summer storms on long-term lake ecosystem dynamics. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.931002 (dataset in review)

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Abstract:
We simulated an experimental summer storm in large-volume (~1200 m3, ~16m depth) enclosures in Lake Stechlin (https://www.lake-lab.de) by mixing deeper water masses from the meta- and hypolimnion into the mixed layer (epilimnion). The mixing included the disturbance of a deep chlorophyll maximum (DCM) which was present at the same time of the experiment in Lake Stechlin and situated in the metalimnion of each enclosure during filling. Size-fractionated Bacterial Protein Production (BPP) of particle associated (PA, >3.0 µm) and free-living bacteria (FL, 0.2-3.0 µm) (14C-Leu incorporation) as well as abundances of PA (microscopy of DAPI stained cells on 3.0 µm polycarbonate filters) and FL heterotrophic prokaryotes and picocyanobacteria (flow cytometry of SYBR green I stained cells) were monitored for 42 days after the experimental disturbance event. Mixing increased bacterial abundance and production about 3 weeks after mixing, which was associated to a mixing-induced stimulation of phytoplankton growth in the mixed enclosures compared to the controls. Simultaneously, decreased abundances of picocyanobacteria could be observed in mixed enclosures.
Keyword(s):
bacterial production; chlorophyll; climate change; cyanobacteria; DCM; disturbance; enclosure experiment; lake; mesocosm experiment; summer storm
Related to:
Grossart, Hans-Peter; Hornick, Thomas; Berger, Stella A; Nejstgaard, Jens Christian; Monaghan, Michael T; Miki, Takashi; Giling, Darren P; Selmeczy, Géza B; Padisák, Judit; Kasprzak, Peter; Wurzbacher, Christian; Kirillin, Georgiy; Engelhardt, Christof; Gessner, Mark O (submitted): Extreme summer storms elicit algal blooms and shift long-term lake ecosystem dynamics.
Funding:
Federal Ministry of Education and Research (BMBF), grant/award no. 01LC1501G: Bridging in Biodiversity Science
Federal Ministry of Education and Research (BMBF), grant/award no. 033L041B: Stickstofflimitation in Binnengewässern
Leibniz Association (Leibniz Association), grant/award no. SAW-2011-IGB-2: Climate driven Changes in Biodiversity of Microbiota
Coverage:
Latitude: 53.143300 * Longitude: 13.028100
Date/Time Start: 2014-08-04T00:00:00 * Date/Time End: 2014-09-16T00:00:00
Minimum DEPTH, water: 2.0 m * Maximum DEPTH, water: 16.0 m
Event(s):
Lake_Stechlin * Latitude: 53.143300 * Longitude: 13.028100 * Location: Germany * Method/Device: Enclosure experiment
Comment:
Empty cells = NA
Further Project information:
Core Facility grant; Award: GE 1775/2-1
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1DATE/TIMEDate/TimeGrossart, Hans-PeterGeocode
2Day of experimentDOEdayGrossart, Hans-Peter
3Oxygen/Nitrogen ratioO2/N2Grossart, Hans-Peter
4DescriptionDescriptionGrossart, Hans-PeterWater layer
5Mesocosm labelMeso labelGrossart, Hans-PeterLakeLab mesocosm number
6TreatmentTreatGrossart, Hans-Peter
7DEPTH, waterDepth watermGrossart, Hans-PeterGeocode
8Protein production, free-living bacteriaFL BPP-Cµg/l/dayGrossart, Hans-Peter14C-leucine incorporation14-C Leucine uptake of size fractionated water sample (0.2-5.0 µm) on Polycarbonate-filters
9Protein production, particle associated bacteriaPA BPP-Cµg/l/dayGrossart, Hans-Peter14C-leucine incorporation14-C Leucine uptake of size fractionated water sample (<5.0 µm) on Polycarbonate-filters
10Bacterial abundance of LDNA-BacteriaBact abund LDNA#Grossart, Hans-PeterFlow cytometry
11Bacterial abundance of HDNA-BacteriaBact abund HDNA#Grossart, Hans-PeterFlow cytometry
12Free-living bacterial abundanceBact abund FL#Grossart, Hans-PeterFlow cytometryDerived from sum of # Bacteria_LDNA and # Bacteria_HDNA
13Particle-associated bacterial abundanceBact abund PA#Grossart, Hans-PeterEpifluorescence microscopy after DAPI stainingMicroscopy of DAPI-stained cells on Polycarbonate-filters
14Picocyanobacteria abundancePikocyanobact abund#Grossart, Hans-PeterFlow cytometry
15Nostocales cell abundanceNostocales cell abund#Grossart, Hans-PeterEpifluorescence microscopy after DAPI stainingMicroscopy of DAPI-stained cells on Polycarbonate-filters
16Nostocales biovolumeNostocales biovµm3/lGrossart, Hans-PeterderivedDerived from # Nostocales by conversion with mesocosm-specific Nostocales cell-volume
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2003 data points

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