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Hornick, Thomas; Mach, Elke; Grossart, Hans-Peter (2021): TemBi 2014 mesocosm study: Summer storm impact on size-fractionated bacterial protein production and abundance of heterotrophic prokaryotes and picocyanobacteria [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.930921, In: Grossart, Hans-Peter; Hornick, Thomas; Berger, Stella A; Nejstgaard, Jens Christian; Monaghan, Michael T; Miki, Takashi; Giling, Darren P; Selmeczy, Géza B; Padisák, Judit; Kasprzak, Peter; Wurzbacher, Christian; Kirillin, Georgiy; Engelhardt, Christof; Gessner, Mark O: TemBi 2014 mesocosm study - Impact of summer storms on long-term lake ecosystem dynamics [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.931002 (dataset in review)

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Abstract:
We simulated an experimental summer storm in large-volume (~1200 m3, ~16m depth) enclosures in Lake Stechlin (https://www.lake-lab.de) by mixing deeper water masses from the meta- and hypolimnion into the mixed layer (epilimnion). The mixing included the disturbance of a deep chlorophyll maximum (DCM) which was present at the same time of the experiment in Lake Stechlin and situated in the metalimnion of each enclosure during filling. Size-fractionated Bacterial Protein Production (BPP) of particle associated (PA, >3.0 µm) and free-living bacteria (FL, 0.2-3.0 µm) (14C-Leu incorporation) as well as abundances of PA (microscopy of DAPI stained cells on 3.0 µm polycarbonate filters) and FL heterotrophic prokaryotes and picocyanobacteria (flow cytometry of SYBR green I stained cells) were monitored for 42 days after the experimental disturbance event. Mixing increased bacterial abundance and production about 3 weeks after mixing, which was associated to a mixing-induced stimulation of phytoplankton growth in the mixed enclosures compared to the controls. Simultaneously, decreased abundances of picocyanobacteria could be observed in mixed enclosures.
Keyword(s):
bacterial production; chlorophyll; Climate change; cyanobacteria; DCM; disturbance; enclosure experiment; lake; mesocosm experiment; summer storm
Related to:
Grossart, Hans-Peter; Hornick, Thomas; Berger, Stella A; Nejstgaard, Jens Christian; Monaghan, Michael T; Miki, Takashi; Giling, Darren P; Selmeczy, Géza B; Padisák, Judit; Kasprzak, Peter; Wurzbacher, Christian; Kirillin, Georgiy; Engelhardt, Christof; Gessner, Mark O (submitted): Extreme summer storms elicit algal blooms and shift long-term lake ecosystem dynamics.
Project(s):
Bridging in Biodiversity Science (BIBS)
Climate driven Changes in Biodiversity of Microbiota (TemBi)
Stickstofflimitation in Binnengewässern (NITROLIMIT)
Funding:
Federal Ministry of Education and Research (BMBF), grant/award no. 01LC1501G: Bridging in Biodiversity Science
Federal Ministry of Education and Research (BMBF), grant/award no. 033L041B: Stickstofflimitation in Binnengewässern
Leibniz Association (Leibniz Association), grant/award no. SAW-2011-IGB-2: Climate driven Changes in Biodiversity of Microbiota
Coverage:
Latitude: 53.143300 * Longitude: 13.028100
Date/Time Start: 2014-08-04T00:00:00 * Date/Time End: 2014-09-16T00:00:00
Minimum DEPTH, water: 2.0 m * Maximum DEPTH, water: 16.0 m
Event(s):
Lake_Stechlin * Latitude: 53.143300 * Longitude: 13.028100 * Location: Germany * Method/Device: Enclosure experiment
Comment:
Empty cells = NA
Further Project information:
Core Facility grant; Award: GE 1775/2-1
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 DATE/TIMEDate/TimeGrossart, Hans-PeterGeocode
2 Day of experimentDOEdayGrossart, Hans-Peter
3 Oxygen/Nitrogen ratioO2/N2Grossart, Hans-Peter
4 DescriptionDescriptionGrossart, Hans-PeterWater layer
5 Mesocosm labelMeso labelGrossart, Hans-PeterLakeLab mesocosm number
6 TreatmentTreatGrossart, Hans-Peter
7 DEPTH, waterDepth watermGrossart, Hans-PeterGeocode
8 Protein production, free-living bacteriaFL BPP-Cµg/l/dayGrossart, Hans-Peter14C-leucine incorporation14-C Leucine uptake of size fractionated water sample (0.2-5.0 µm) on Polycarbonate-filters
9 Protein production, particle associated bacteriaPA BPP-Cµg/l/dayGrossart, Hans-Peter14C-leucine incorporation14-C Leucine uptake of size fractionated water sample (<5.0 µm) on Polycarbonate-filters
10 Bacterial abundance of LDNA-BacteriaBact abund LDNA#Grossart, Hans-PeterFlow cytometry
11 Bacterial abundance of HDNA-BacteriaBact abund HDNA#Grossart, Hans-PeterFlow cytometry
12 Free-living bacterial abundanceBact abund FL#Grossart, Hans-PeterFlow cytometryDerived from sum of # Bacteria_LDNA and # Bacteria_HDNA
13 Particle-associated bacterial abundanceBact abund PA#Grossart, Hans-PeterEpifluorescence microscopy after DAPI stainingMicroscopy of DAPI-stained cells on Polycarbonate-filters
14 Picocyanobacteria abundancePikocyanobact abund#Grossart, Hans-PeterFlow cytometry
15 Nostocales cell abundanceNostocales cell abund#Grossart, Hans-PeterEpifluorescence microscopy after DAPI stainingMicroscopy of DAPI-stained cells on Polycarbonate-filters
16 Nostocales biovolumeNostocales biovµm3/lGrossart, Hans-PeterderivedDerived from # Nostocales by conversion with mesocosm-specific Nostocales cell-volume
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2003 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Date/Time		2 
DOE [day]		3 
O2/N2		4 
Description		5 
Meso label		6 
Treat		7 
Depth water [m]		8 
FL BPP-C [µg/l/day]		9 
PA BPP-C [µg/l/day]		10 
Bact abund LDNA [#]		11 
Bact abund HDNA [#]		12 
Bact abund FL [#]		13 
Bact abund PA [#]		14 
Pikocyanobact abund [#]		15 
Nostocales cell abund [#]		16 
Nostocales biov [µm3/l]
2014-08-04-1216epilimnion1control2.018.421.91161100312700147380041553899500
2014-08-061218epilimnion1control2.012.110.516875672314671919033206944124933
2014-08-083220epilimnion1control2.029.517.91787700267900205560023229782700
2014-08-116223epilimnion1control2.012.613.4152171415571416774297942593714
2014-08-1510227epilimnion1control2.0
2014-08-1914231epilimnion1control2.016.910.41843900297400214130027166672900
2014-08-2621238epilimnion1control2.011.311.618606002433002103900102341464008767014
2014-09-0127244epilimnion1control2.010.311.11621200221200184240018497629200
2014-09-0935252epilimnion1control2.0
2014-09-1642259epilimnion1control2.09.013.7149815018255016807005540519050
2014-08-04-1216epilimnion3control2.018.818.815087003974001906100274964128800
2014-08-061218epilimnion3control2.012.813.417371332220001959133194577127667
2014-08-083220epilimnion3control2.015.016.51572200279400185160019746373700
2014-08-116223epilimnion3control2.014.212.318164291935712010000105671144643
2014-08-1510227epilimnion3control2.0
2014-08-1914231epilimnion3control2.017.112.41844600263800210840034648884600
2014-08-2621238epilimnion3control2.014.213.11992700225700221840010015149600954189
2014-09-0127244epilimnion3control2.013.512.11894700244200213890026175645700
2014-09-0935252epilimnion3control2.0
2014-09-1642259epilimnion3control2.013.110.71533200146850168005010698224550
2014-08-04-1216epilimnion5treatment2.017.212.914097003780001787700294339127800
2014-08-061218epilimnion5treatment2.07.17.115440332527001796733270704104800
2014-08-083220epilimnion5treatment2.016.219.41518200339000185720034483956300
2014-08-116223epilimnion5treatment2.016.115.6176892923957120085004836955071
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2014-09-0935252epilimnion5treatment2.0
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2014-08-04-1216epilimnion8control2.012.011.51166400364600153100037926190100
2014-08-061218epilimnion8control2.012.116.61494067226300172036719457761233
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2014-08-04-1216epilimnion13treatment2.08.913.31089900328000141790048232197200
2014-08-061218epilimnion13treatment2.023.720.217992002462002045400191829128000
2014-08-083220epilimnion13treatment2.016.116.41693000267900196090026939988200
2014-08-116223epilimnion13treatment2.013.019.61899286192000209128696327109857
2014-08-1510227epilimnion13treatment2.0
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2014-08-2621238epilimnion13treatment2.012.711.5196680016800021348001268574240000
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2014-09-0935252epilimnion13treatment2.0
2014-09-1642259epilimnion13treatment2.014.510.8165345019810018515508789046700
2014-08-04-1216epilimnion15control2.025.422.31049700254000130370034277754400
2014-08-061218epilimnion15control2.013.913.71470167203700167386719732562767
2014-08-083220epilimnion15control2.015.717.51309500172000148150030196642000
2014-08-116223epilimnion15control2.014.214.2169007123700019270718547197000
2014-08-1510227epilimnion15control2.0
2014-08-1914231epilimnion15control2.015.011.720426003583002400900365863166700
2014-08-2621238epilimnion15control2.014.310.2203810025970022978008986818890000
2014-09-0127244epilimnion15control2.013.410.819864004189002405300129993177200
2014-09-0935252epilimnion15control2.0
2014-09-1642259epilimnion15control2.010.813.11419200174700159390011987937900
2014-08-04-1216epilimnion16treatment2.016.515.4870400218300108870047861053200
2014-08-061218epilimnion16treatment2.015.212.01487733247800173553322364071167
2014-08-083220epilimnion16treatment2.018.012.11227700214100144180026692521700
2014-08-116223epilimnion16treatment2.019.412.1156528620728617725718767021857
2014-08-1510227epilimnion16treatment2.0
2014-08-1914231epilimnion16treatment2.018.510.51988300429000241730030052315600
2014-08-2621238epilimnion16treatment2.037.214.816854002674001952800262929144002635657680
2014-09-0127244epilimnion16treatment2.023.214.41689400302000199140026089929600
2014-09-0935252epilimnion16treatment2.0
2014-09-1642259epilimnion16treatment2.012.220.01565200190950175615016340747900
2014-08-04-1216epilimnion17treatment2.020.115.375860019690095550026239150700
2014-08-061218epilimnion17treatment2.020.712.91449633262367171200023930569500
2014-08-083220epilimnion17treatment2.016.510.31188000133300132130030670629400
2014-08-116223epilimnion17treatment2.018.39.2138328619485715781438148618143
2014-08-1510227epilimnion17treatment2.0
2014-08-1914231epilimnion17treatment2.016.110.22208700348700255740043656229000
2014-08-2621238epilimnion17treatment2.024.712.617928002781002070900297470310003787034804
2014-09-0127244epilimnion17treatment2.018.917.51621300236400185770021554151200
2014-09-0935252epilimnion17treatment2.0
2014-09-1642259epilimnion17treatment2.012.911.51602650256300185895012070485350
2014-08-04-1216epilimnionL1lake2.01719300461900218120079356152800
2014-08-061218epilimnionL1lake2.022824673263332608800176733
2014-08-083220epilimnionL1lake2.015322001885001720700102400
2014-08-116223epilimnionL1lake2.020022142575712259786207286
2014-08-1510227epilimnionL1lake2.0
2014-08-1914231epilimnionL1lake2.023075004500002757500146000
2014-08-2621238epilimnionL1lake2.020436003269002370500110000
2014-09-0127244epilimnionL1lake2.01561300173800173510078100
2014-09-0935252epilimnionL1lake2.0
2014-09-1642259epilimnionL1lake2.01526350171800169815062350
2014-08-04-1216hypolimnion1control16.010.711.2
2014-08-061218hypolimnion1control16.07.76.5
2014-08-083220hypolimnion1control16.015.011.2
2014-08-116223hypolimnion1control16.06.89.4
2014-08-1510227hypolimnion1control16.0
2014-08-1914231hypolimnion1control16.011.77.8
2014-08-2621238hypolimnion1control16.012.124.4
2014-09-0127244hypolimnion1control16.08.711.4
2014-09-0935252hypolimnion1control16.0
2014-09-1642259hypolimnion1control16.014.113.6
2014-08-04-1216hypolimnion3control16.016.410.0
2014-08-061218hypolimnion3control16.015.67.3
2014-08-083220hypolimnion3control16.012.210.6
2014-08-116223hypolimnion3control16.08.26.2
2014-08-1510227hypolimnion3control16.0
2014-08-1914231hypolimnion3control16.014.38.5
2014-08-2621238hypolimnion3control16.015.817.4
2014-09-0127244hypolimnion3control16.011.98.8
2014-09-0935252hypolimnion3control16.0
2014-09-1642259hypolimnion3control16.09.88.3
2014-08-04-1216hypolimnion5treatment16.017.111.8
2014-08-061218hypolimnion5treatment16.013.810.5
2014-08-083220hypolimnion5treatment16.010.18.8
2014-08-116223hypolimnion5treatment16.010.28.6
2014-08-1510227hypolimnion5treatment16.0
2014-08-1914231hypolimnion5treatment16.08.17.6
2014-08-2621238hypolimnion5treatment16.011.012.2
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2014-09-0935252hypolimnion5treatment16.0
2014-09-1642259hypolimnion5treatment16.09.79.9
2014-08-04-1216hypolimnion8control16.017.59.4
2014-08-061218hypolimnion8control16.013.38.2
2014-08-083220hypolimnion8control16.012.610.0
2014-08-116223hypolimnion8control16.08.18.6
2014-08-1510227hypolimnion8control16.0
2014-08-1914231hypolimnion8control16.016.28.3
2014-08-2621238hypolimnion8control16.012.511.1
2014-09-0127244hypolimnion8control16.011.38.0
2014-09-0935252hypolimnion8control16.0
2014-09-1642259hypolimnion8control16.07.19.4
2014-08-04-1216hypolimnion13treatment16.06.98.4
2014-08-061218hypolimnion13treatment16.014.46.8
2014-08-083220hypolimnion13treatment16.08.47.0
2014-08-116223hypolimnion13treatment16.09.17.1
2014-08-1510227hypolimnion13treatment16.0
2014-08-1914231hypolimnion13treatment16.07.36.1
2014-08-2621238hypolimnion13treatment16.012.914.4
2014-09-0127244hypolimnion13treatment16.06.07.7
2014-09-0935252hypolimnion13treatment16.0
2014-09-1642259hypolimnion13treatment16.09.55.7
2014-08-04-1216hypolimnion15control16.016.915.1
2014-08-061218hypolimnion15control16.016.28.7
2014-08-083220hypolimnion15control16.015.514.4
2014-08-116223hypolimnion15control16.010.810.8
2014-08-1510227hypolimnion15control16.0
2014-08-1914231hypolimnion15control16.018.212.1
2014-08-2621238hypolimnion15control16.022.720.2
2014-09-0127244hypolimnion15control16.015.811.7
2014-09-0935252hypolimnion15control16.0
2014-09-1642259hypolimnion15control16.07.210.4
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2014-08-1914231hypolimnion16treatment16.08.26.1
2014-08-2621238hypolimnion16treatment16.014.211.6
2014-09-0127244hypolimnion16treatment16.011.64.8
2014-09-0935252hypolimnion16treatment16.0
2014-09-1642259hypolimnion16treatment16.09.110.7
2014-08-04-1216hypolimnion17treatment16.023.010.8
2014-08-061218hypolimnion17treatment16.019.910.9
2014-08-083220hypolimnion17treatment16.016.511.0
2014-08-116223hypolimnion17treatment16.010.56.6
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2014-08-1914231hypolimnion17treatment16.010.28.3
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2014-09-0935252hypolimnion17treatment16.0
2014-09-1642259hypolimnion17treatment16.09.59.2
2014-08-04-1216metalimnion1control7.518.315.3
2014-08-061218metalimnion1control7.516.312.8
2014-08-083220metalimnion1control7.514.915.1
2014-08-116223metalimnion1control7.57.610.2
2014-08-1510227metalimnion1control7.5
2014-08-1914231metalimnion1control9.010.714.0
2014-08-2621238metalimnion1control9.510.810.7
2014-09-0127244metalimnion1control9.58.610.0
2014-09-0935252metalimnion1control10.0
2014-09-1642259metalimnion1control10.017.921.9
2014-08-04-1216metalimnion3control7.520.317.8
2014-08-061218metalimnion3control7.514.58.2
2014-08-083220metalimnion3control7.516.015.4
2014-08-116223metalimnion3control7.516.010.5
2014-08-1510227metalimnion3control7.5
2014-08-1914231metalimnion3control9.012.17.8
2014-08-2621238metalimnion3control9.514.612.5
2014-09-0127244metalimnion3control9.512.610.2
2014-09-0935252metalimnion3control10.0
2014-09-1642259metalimnion3control10.023.233.7
2014-08-04-1216metalimnion5treatment7.56.99.8
2014-08-061218metalimnion5treatment7.514.812.0
2014-08-083220metalimnion5treatment7.510.913.2
2014-08-116223metalimnion5treatment7.513.310.5
2014-08-1510227metalimnion5treatment7.5
2014-08-1914231metalimnion5treatment9.09.67.6
2014-08-2621238metalimnion5treatment9.516.411.6
2014-09-0127244metalimnion5treatment9.58.47.0
2014-09-0935252metalimnion5treatment10.0
2014-09-1642259metalimnion5treatment10.09.212.2
2014-08-04-1216metalimnion8control7.512.615.9
2014-08-061218metalimnion8control7.514.510.9
2014-08-083220metalimnion8control7.513.111.4
2014-08-116223metalimnion8control7.511.911.5
2014-08-1510227metalimnion8control7.5
2014-08-1914231metalimnion8control9.015.89.6
2014-08-2621238metalimnion8control9.511.911.1
2014-09-0127244metalimnion8control9.57.610.7
2014-09-0935252metalimnion8control10.0
2014-09-1642259metalimnion8control10.021.521.9
2014-08-04-1216metalimnion13treatment7.512.715.6
2014-08-061218metalimnion13treatment7.510.39.6
2014-08-083220metalimnion13treatment7.511.111.1
2014-08-116223metalimnion13treatment7.510.114.4
2014-08-1510227metalimnion13treatment7.5
2014-08-1914231metalimnion13treatment9.08.36.9
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2014-09-0127244metalimnion13treatment9.56.28.6
2014-09-0935252metalimnion13treatment10.0
2014-09-1642259metalimnion13treatment10.011.813.3
2014-08-04-1216metalimnion15control7.514.215.6
2014-08-061218metalimnion15control7.510.69.5
2014-08-083220metalimnion15control7.514.313.2
2014-08-116223metalimnion15control7.510.912.3
2014-08-1510227metalimnion15control7.5
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2014-09-0127244metalimnion15control9.511.59.1
2014-09-0935252metalimnion15control10.0
2014-09-1642259metalimnion15control10.017.622.7
2014-08-04-1216metalimnion16treatment7.513.812.4
2014-08-061218metalimnion16treatment7.512.28.2
2014-08-083220metalimnion16treatment7.515.312.0
2014-08-116223metalimnion16treatment7.511.59.6
2014-08-1510227metalimnion16treatment7.5
2014-08-1914231metalimnion16treatment9.011.48.4
2014-08-2621238metalimnion16treatment9.524.713.0
2014-09-0127244metalimnion16treatment9.514.16.9
2014-09-0935252metalimnion16treatment10.0
2014-09-1642259metalimnion16treatment10.07.014.5
2014-08-04-1216metalimnion17treatment7.514.810.9
2014-08-061218metalimnion17treatment7.515.510.5
2014-08-083220metalimnion17treatment7.515.513.3
2014-08-116223metalimnion17treatment7.515.69.6
2014-08-1510227metalimnion17treatment7.5
2014-08-1914231metalimnion17treatment9.09.77.0
2014-08-2621238metalimnion17treatment9.519.114.0
2014-09-0127244metalimnion17treatment9.58.77.5
2014-09-0935252metalimnion17treatment10.0
2014-09-1642259metalimnion17treatment10.010.714.2