Citation:

PANGAEA.929201

Name: Seawater carbonate chemistry and polyspermy of a broadcast spawning bivalve
Published: 2021-03-16
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Laboratory experiment; Mollusca; North Pacific; Reproduction; Single species; Tegillarca granosa; Temperate

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Abstract:

Ensuring that oocytes are fertilized by a single sperm during broadcast spawning is crucial for the fertilization success of many marine invertebrates. Although the adverse impacts of ocean acidification (OA) on various marine species have been revealed in recent years, its impact on polyspermy and the underlying mechanisms involved remain largely unknown. Therefore, in the present study, the effect of OA on polyspermy risk was assessed in a broadcast spawning bivalve, Tegillarca granosa. In addition, the impacts of OA on the two polyspermy blocking processes, the fast block (membrane depolarization) and the permanent block (cortical reaction), were investigated. The results show that the exposure of oocytes to two future OA scenarios (pH 7.8 and pH 7.4) leads to significant increases in polyspermy risk, about 1.70 and 2.38 times higher than the control, respectively. The maximum change in the membrane potential during oocyte membrane depolarization markedly decreased to 15.79% (pH 7.8) and 34.06% (pH 7.4) of the control value. Moreover, the duration of oocyte membrane depolarization was significantly reduced to approximately 63.38% (pH 7.8) and 21.91% (pH 7.4) of the control. In addition, cortical granule exocytosis, as well as microfilament migration, were significantly arrested by OA treatment. Exposure to future OA scenarios also led to significant reductions in the ATP and Ca2+ content of the oocytes, which may explain the hampered polyspermy blocking. Overall, the present study suggests that OA may significantly increase polyspermy risk in T. granosa by inhibiting membrane depolarization and arresting cortical granule exocytosis.

Keyword(s):

Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Laboratory experiment; Mollusca; North Pacific; Reproduction; Single species; Tegillarca granosa; Temperate

Supplement to:

Han, Yu; Shi, Wei; Tang, Y; Zhao, Xinguo; Du, Xueying; Sun, Shuge; Zhou, Weishang; Liu, Guangxu (2021): Ocean acidification increases polyspermy of a broadcast spawning bivalve species by hampering membrane depolarization and cortical granule exocytosis. Aquatic Toxicology, 231, 105740, https://doi.org/10.1016/j.aquatox.2020.105740

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 28.283500 * Longitude: 121.244300

Event(s):

Yueqing_Bay_OA * Latitude: 28.283500 * Longitude: 121.244300 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-03-15.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Liu, Guangxu		study
2	Species	Species		Liu, Guangxu
3	Registration number of species	Reg spec no		Liu, Guangxu
4	Uniform resource locator/link to reference	URL ref		Liu, Guangxu		WoRMS Aphia ID
5	Treatment	Treat		Liu, Guangxu
6	Polyspermy	Polyspermy	%	Liu, Guangxu
7	Relative fluorescence intensity, ratio	Q		Liu, Guangxu		Peak
8	Time in seconds	Time	s	Liu, Guangxu		Membrane depolarization
9	Fluorescence intensity	Fluorescence		Liu, Guangxu		Cortical granules
10	Microfilament migration	Microfilament mig	%	Liu, Guangxu		The outer-layer to whole-cell ratio of the fluorescent intensity
11	Adenosine triphosphate, per unit protein	ATP	nmol/mg	Liu, Guangxu
12	Fluorescence intensity	Fluorescence		Liu, Guangxu		Ca2+ content
13	Temperature, water	Temp	°C	Liu, Guangxu
14	Temperature, water, standard deviation	Temp std dev	±	Liu, Guangxu
15	Salinity	Sal		Liu, Guangxu
16	Salinity, standard deviation	Sal std dev	±	Liu, Guangxu
17	pH	pH		Liu, Guangxu	Potentiometric	NBS scale
18	pH, standard deviation	pH std dev	±	Liu, Guangxu	Potentiometric	NBS scale
19	Alkalinity, total	AT	µmol/kg	Liu, Guangxu	Potentiometric titration
20	Alkalinity, total, standard deviation	AT std dev	±	Liu, Guangxu	Potentiometric titration
21	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Liu, Guangxu	Calculated using CO2SYS
22	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Liu, Guangxu	Calculated using CO2SYS
23	Carbon, inorganic, dissolved	DIC	µmol/kg	Liu, Guangxu	Calculated using CO2SYS
24	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Liu, Guangxu	Calculated using CO2SYS
25	Aragonite saturation state	Omega Arg		Liu, Guangxu	Calculated using CO2SYS
26	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Liu, Guangxu	Calculated using CO2SYS
27	Calcite saturation state	Omega Cal		Liu, Guangxu	Calculated using CO2SYS
28	Calcite saturation state, standard deviation	Omega Cal std dev	±	Liu, Guangxu	Calculated using CO2SYS
29	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
31	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3744 data points

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