Citation:

PANGAEA.929201

Name: Seawater carbonate chemistry and polyspermy of a broadcast spawning bivalve
Published: 2021-03-16
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Laboratory experiment; Mollusca; North Pacific; Reproduction; Single species; Tegillarca granosa; Temperate

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook TwitterShow Map Google Earth

Abstract:

Ensuring that oocytes are fertilized by a single sperm during broadcast spawning is crucial for the fertilization success of many marine invertebrates. Although the adverse impacts of ocean acidification (OA) on various marine species have been revealed in recent years, its impact on polyspermy and the underlying mechanisms involved remain largely unknown. Therefore, in the present study, the effect of OA on polyspermy risk was assessed in a broadcast spawning bivalve, Tegillarca granosa. In addition, the impacts of OA on the two polyspermy blocking processes, the fast block (membrane depolarization) and the permanent block (cortical reaction), were investigated. The results show that the exposure of oocytes to two future OA scenarios (pH 7.8 and pH 7.4) leads to significant increases in polyspermy risk, about 1.70 and 2.38 times higher than the control, respectively. The maximum change in the membrane potential during oocyte membrane depolarization markedly decreased to 15.79% (pH 7.8) and 34.06% (pH 7.4) of the control value. Moreover, the duration of oocyte membrane depolarization was significantly reduced to approximately 63.38% (pH 7.8) and 21.91% (pH 7.4) of the control. In addition, cortical granule exocytosis, as well as microfilament migration, were significantly arrested by OA treatment. Exposure to future OA scenarios also led to significant reductions in the ATP and Ca2+ content of the oocytes, which may explain the hampered polyspermy blocking. Overall, the present study suggests that OA may significantly increase polyspermy risk in T. granosa by inhibiting membrane depolarization and arresting cortical granule exocytosis.

Keyword(s):

Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Laboratory experiment; Mollusca; North Pacific; Reproduction; Single species; Tegillarca granosa; Temperate

Supplement to:

Han, Yu; Shi, Wei; Tang, Y; Zhao, Xinguo; Du, Xueying; Sun, Shuge; Zhou, Weishang; Liu, Guangxu (2021): Ocean acidification increases polyspermy of a broadcast spawning bivalve species by hampering membrane depolarization and cortical granule exocytosis. Aquatic Toxicology, 231, 105740, https://doi.org/10.1016/j.aquatox.2020.105740

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 28.283500 * Longitude: 121.244300

Event(s):

Yueqing_Bay_OA * Latitude: 28.283500 * Longitude: 121.244300 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-03-15.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Liu, Guangxu		study
2	Species	Species		Liu, Guangxu
3	Registration number of species	Reg spec no		Liu, Guangxu
4	Uniform resource locator/link to reference	URL ref		Liu, Guangxu		WoRMS Aphia ID
5	Treatment	Treat		Liu, Guangxu
6	Polyspermy	Polyspermy	%	Liu, Guangxu
7	Relative fluorescence intensity, ratio	Q		Liu, Guangxu		Peak
8	Time in seconds	Time	s	Liu, Guangxu		Membrane depolarization
9	Fluorescence intensity	Fluorescence		Liu, Guangxu		Cortical granules
10	Microfilament migration	Microfilament mig	%	Liu, Guangxu		The outer-layer to whole-cell ratio of the fluorescent intensity
11	Adenosine triphosphate, per unit protein	ATP	nmol/mg	Liu, Guangxu
12	Fluorescence intensity	Fluorescence		Liu, Guangxu		Ca2+ content
13	Temperature, water	Temp	°C	Liu, Guangxu
14	Temperature, water, standard deviation	Temp std dev	±	Liu, Guangxu
15	Salinity	Sal		Liu, Guangxu
16	Salinity, standard deviation	Sal std dev	±	Liu, Guangxu
17	pH	pH		Liu, Guangxu	Potentiometric	NBS scale
18	pH, standard deviation	pH std dev	±	Liu, Guangxu	Potentiometric	NBS scale
19	Alkalinity, total	AT	µmol/kg	Liu, Guangxu	Potentiometric titration
20	Alkalinity, total, standard deviation	AT std dev	±	Liu, Guangxu	Potentiometric titration
21	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Liu, Guangxu	Calculated using CO2SYS
22	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Liu, Guangxu	Calculated using CO2SYS
23	Carbon, inorganic, dissolved	DIC	µmol/kg	Liu, Guangxu	Calculated using CO2SYS
24	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Liu, Guangxu	Calculated using CO2SYS
25	Aragonite saturation state	Omega Arg		Liu, Guangxu	Calculated using CO2SYS
26	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Liu, Guangxu	Calculated using CO2SYS
27	Calcite saturation state	Omega Cal		Liu, Guangxu	Calculated using CO2SYS
28	Calcite saturation state, standard deviation	Omega Cal std dev	±	Liu, Guangxu	Calculated using CO2SYS
29	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
31	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3744 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Treat	6 Polyspermy [%]	7 Q (Peak)	8 Time [s] (Membrane depolarization)	9 Fluorescence (Cortical granules)	10 Microfilament mig [%] (The outer-layer to whole-cell...)	11 ATP [nmol/mg]	12 Fluorescence (Ca2+ content)	13 Temp [°C]	14 Temp std dev [±]	15 Sal	16 Sal std dev [±]	17 pH (NBS scale, Potentiometric)	18 pH std dev [±] (NBS scale, Potentiometric)	19 AT [µmol/kg] (Potentiometric titration)	20 AT std dev [±] (Potentiometric titration)	21 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	22 pCO2 std dev [±] (Calculated using CO2SYS)	23 DIC [µmol/kg] (Calculated using CO2SYS)	24 DIC std dev [±] (Calculated using CO2SYS)	25 Omega Arg (Calculated using CO2SYS)	26 Omega Arg std dev [±] (Calculated using CO2SYS)	27 Omega Cal (Calculated using CO2SYS)	28 Omega Cal std dev [±] (Calculated using CO2SYS)	29 CSC flag (Calculated using seacarb afte...)	30 pH (total scale, Calculated using...)	31 CO2 [µmol/kg] (Calculated using seacarb afte...)	32 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	33 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	34 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	35 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	36 DIC [µmol/kg] (Calculated using seacarb afte...)	37 Omega Arg (Calculated using seacarb afte...)	38 Omega Cal (Calculated using seacarb afte...)
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	19.31034							23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	17.58621							23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	9.90415							23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	9.45122							23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	11.00000							23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	15.87302							23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	19.56522							23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	24.33333							23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	9.04762							23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	12.65823							23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	25.74257							23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	29.67742							23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	20.33898							23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	22.14984							23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	23.93617							23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	25.94595							23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	37.12871							23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	30.00000							23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	17.36527							23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	20.00000							23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	33.58209							24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	36.85345							24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	38.26090							24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	33.78750							24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	32.48408							24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	31.67260							24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	43.11377							24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	39.36170							24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	31.14187							24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	33.23864							24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1		1.37731						23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1		1.73267						23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1		1.43989						23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8		1.15252						23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8		1.44394						23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8		1.23518						23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4		1.17876						24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4		1.08467						24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4		1.17735						24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1			9.00					23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1			6.30					23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1			11.40					23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8			5.10					23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8			3.70					23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8			8.10					23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4			1.20					24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4			1.20					24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4			3.45					24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1				0.01991				23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1				0.02164				23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1				0.02237				23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1				0.02064				23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1				0.01809				23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1				0.02261				23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8				0.03218				23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8				0.03007				23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8				0.03823				23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8				0.03287				23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8				0.03679				23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8				0.03621				23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4				0.03930				24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4				0.03326				24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4				0.03936				24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4				0.03696				24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4				0.03313				24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4				0.04408				24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1					74.27897			23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1					77.62448			23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1					79.51198			23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1					94.52659			23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1					85.89675			23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1					75.30224			23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1					74.55337			23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8					41.49120			23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8					42.97858			23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8					54.03252			23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8					38.21268			23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8					53.24445			23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8					50.90707			23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8					46.53967			23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4					42.37237			24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4					44.41841			24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4					39.32419			24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4					31.25352			24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4					39.85948			24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4					39.78856			24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4					39.75052			24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1						1695.65217		23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1						1086.95652		23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1						1043.47826		23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1						1260.86957		23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1						1413.04348		23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8						956.52174		23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8						913.04348		23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8						869.56522		23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8						804.34783		23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8						1152.17391		23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4						500.00000		24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4						717.39130		24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4						673.91304		24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4						695.65217		24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4						543.47826		24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1							0.14917	23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1							0.11310	23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1							0.09948	23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1							0.12531	23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1							0.10008	23.9	0.2	20.68	0.24	8.13	0.04	2061.98	20.46	496.38	53.11	1933.05	23.31	1.75	0.17	2.99	0.25	26	7.98	15.77	500.92	502.55	1777.60	121.03	1914.40	2.02	3.24
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8							0.10721	23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8							0.09382	23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8							0.11888	23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8							0.05175	23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8							0.09284	23.7	0.3	20.66	0.16	7.80	0.03	2066.41	26.87	1129.29	82.68	2032.26	28.99	0.88	0.05	1.42	0.04	26	7.65	36.60	1156.56	1160.33	1923.47	60.82	2020.90	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4							0.08320	24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4							0.04917	24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4							0.03218	24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4							0.08125	24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4							0.07118	24.0	0.1	20.64	0.15	7.41	0.01	2071.22	20.85	2906.98	88.90	2141.85	21.67	0.37	0.01	0.59	0.01	26	7.26	93.48	2976.93	2986.61	2009.89	26.13	2129.50	0.44	0.70