Citation:

PANGAEA.920548

Name: Seawater carbonate chemistry and larval response to parental low pH exposure in the pacific oyster Crassostrea gigas
Published: 2019
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Laboratory experiment; Mollusca; North Atlantic; Pelagos; Reproduction; Single species; Temperate; Zooplankton

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Abstract:

As negative effects of ocean acidification are experienced by coastal ecosystems, there is a growing trend to investigate the effect ocean acidification has on multiple generations. Parental exposure to ocean acidification has been shown to induce larval carryover effects, but whether acute exposure to a stressor as an adult can influence the larval generation long after the stress has been removed has yet to be tested. To assess how a temporary exposure to experimental ocean acidification affects the ecologically and commercially relevant Pacific oyster Crassostrea gigas, adult oysters were exposed to either low pH (7.31 +- 0.02) or ambient pH (7.82 +- 0.02) conditions for 7 wk. Oysters were then held for 8 wk in ambient conditions, and subsequently reproductively conditioned for 4 wk at ambient pH. After conditioning, the oysters were strip-spawned to create four families based on maternal and paternal ocean acidification exposure. The number of D-hinge larvae was counted 18 h postfertilization. A sex-specific brood stock response was observed, where female exposure to low pH conditions resulted in fewer D-hinge larvae. This study demonstrates that the effects of ocean acidification can last beyond the time from when the environmental perturbation is experienced. Broadening the understanding of environmental memory will be valuable when considering organismal ability to persist in the face of environmental change.

Keyword(s):

Animalia; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Laboratory experiment; Mollusca; North Atlantic; Pelagos; Reproduction; Single species; Temperate; Zooplankton

Supplement to:

Venkataraman, Yaamini R; Spencer, Laura H; Roberts, Steven B (2019): Larval Response to Parental Low pH Exposure in the Pacific Oyster Crassostrea gigas. Journal of Shellfish Research, 38(3), 743, https://doi.org/10.2983/035.038.0325

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-07-07.The “Female” and “Male” notes for the carbonate chemistry data reflect the use of planned crosses to create half-sibling families and the subsequent investigation of male and female carryover effects on larvae. However, the males and females were held in the same tanks for the 7-week pH experiment. Additionally, the larvae themselves were spawned and reared in ambient pH conditions four months after the adult exposure ended.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Venkataraman, Yaamini R		study
2	Species	Species		Venkataraman, Yaamini R
3	Registration number of species	Reg spec no		Venkataraman, Yaamini R
4	Uniform resource locator/link to reference	URL ref		Venkataraman, Yaamini R		WoRMS Aphia ID
5	Identification	ID		Venkataraman, Yaamini R		Bucket IDs for two replicates with identical cross
6	Volume	Vol	ml	Venkataraman, Yaamini R		Pool used for spawn
7	Eggs	Eggs	#	Venkataraman, Yaamini R		used for spawn
8	Larvae	Larvae	#	Venkataraman, Yaamini R		D-Hinge-Count-1
9	Volume	Vol	ml	Venkataraman, Yaamini R		sample used for d-hinge count
10	Larvae	Larvae	#	Venkataraman, Yaamini R		D-Hinge-Count-2
11	Volume	Vol	ml	Venkataraman, Yaamini R		sample used for d-hinge count
12	Larvae	Larvae	#	Venkataraman, Yaamini R		D-Hinge-Count-3
13	Volume	Vol	ml	Venkataraman, Yaamini R		sample used for d-hinge count
14	Larvae	Larvae	#	Venkataraman, Yaamini R		average of three counts
15	Volume	V	µl	Venkataraman, Yaamini R		total of tripour with larvae
16	Larvae	Larvae	#	Venkataraman, Yaamini R		d-hinge larvae produced by each cross
17	Hatching rate	Hatching	%	Venkataraman, Yaamini R		D-Hinge in tripour from total number of eggs used
18	Treatment	Treat		Venkataraman, Yaamini R		Parental
19	Treatment	Treat		Venkataraman, Yaamini R		Female
20	Treatment	Treat		Venkataraman, Yaamini R		Male
21	Identification	ID		Venkataraman, Yaamini R		Sire
22	Alkalinity, total	AT	µmol/kg	Venkataraman, Yaamini R		Female
23	pH	pH		Venkataraman, Yaamini R		total scale, Female
24	Salinity	Sal		Venkataraman, Yaamini R		Female
25	Temperature, water	Temp	°C	Venkataraman, Yaamini R		Female
26	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
27	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
28	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
29	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
30	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
31	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
32	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
33	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
34	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
35	Alkalinity, total	AT	µmol/kg	Venkataraman, Yaamini R		Male
36	pH	pH		Venkataraman, Yaamini R		total scale, male
37	Salinity	Sal		Venkataraman, Yaamini R		Male
38	Temperature, water	Temp	°C	Venkataraman, Yaamini R		Male
39	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male
40	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male
41	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male
42	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male
43	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male
44	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male
45	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male
46	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male
47	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1170 data points

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