Citation:

PANGAEA.920548

Name: Seawater carbonate chemistry and larval response to parental low pH exposure in the pacific oyster Crassostrea gigas
Published: 2019
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Laboratory experiment; Mollusca; North Atlantic; Pelagos; Reproduction; Single species; Temperate; Zooplankton

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Abstract:

As negative effects of ocean acidification are experienced by coastal ecosystems, there is a growing trend to investigate the effect ocean acidification has on multiple generations. Parental exposure to ocean acidification has been shown to induce larval carryover effects, but whether acute exposure to a stressor as an adult can influence the larval generation long after the stress has been removed has yet to be tested. To assess how a temporary exposure to experimental ocean acidification affects the ecologically and commercially relevant Pacific oyster Crassostrea gigas, adult oysters were exposed to either low pH (7.31 +- 0.02) or ambient pH (7.82 +- 0.02) conditions for 7 wk. Oysters were then held for 8 wk in ambient conditions, and subsequently reproductively conditioned for 4 wk at ambient pH. After conditioning, the oysters were strip-spawned to create four families based on maternal and paternal ocean acidification exposure. The number of D-hinge larvae was counted 18 h postfertilization. A sex-specific brood stock response was observed, where female exposure to low pH conditions resulted in fewer D-hinge larvae. This study demonstrates that the effects of ocean acidification can last beyond the time from when the environmental perturbation is experienced. Broadening the understanding of environmental memory will be valuable when considering organismal ability to persist in the face of environmental change.

Keyword(s):

Animalia; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Laboratory experiment; Mollusca; North Atlantic; Pelagos; Reproduction; Single species; Temperate; Zooplankton

Supplement to:

Venkataraman, Yaamini R; Spencer, Laura H; Roberts, Steven B (2019): Larval Response to Parental Low pH Exposure in the Pacific Oyster Crassostrea gigas. Journal of Shellfish Research, 38(3), 743, https://doi.org/10.2983/035.038.0325

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-07-07.The “Female” and “Male” notes for the carbonate chemistry data reflect the use of planned crosses to create half-sibling families and the subsequent investigation of male and female carryover effects on larvae. However, the males and females were held in the same tanks for the 7-week pH experiment. Additionally, the larvae themselves were spawned and reared in ambient pH conditions four months after the adult exposure ended.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Venkataraman, Yaamini R		study
2	Species	Species		Venkataraman, Yaamini R
3	Registration number of species	Reg spec no		Venkataraman, Yaamini R
4	Uniform resource locator/link to reference	URL ref		Venkataraman, Yaamini R		WoRMS Aphia ID
5	Identification	ID		Venkataraman, Yaamini R		Bucket IDs for two replicates with identical cross
6	Volume	Vol	ml	Venkataraman, Yaamini R		Pool used for spawn
7	Eggs	Eggs	#	Venkataraman, Yaamini R		used for spawn
8	Larvae	Larvae	#	Venkataraman, Yaamini R		D-Hinge-Count-1
9	Volume	Vol	ml	Venkataraman, Yaamini R		sample used for d-hinge count
10	Larvae	Larvae	#	Venkataraman, Yaamini R		D-Hinge-Count-2
11	Volume	Vol	ml	Venkataraman, Yaamini R		sample used for d-hinge count
12	Larvae	Larvae	#	Venkataraman, Yaamini R		D-Hinge-Count-3
13	Volume	Vol	ml	Venkataraman, Yaamini R		sample used for d-hinge count
14	Larvae	Larvae	#	Venkataraman, Yaamini R		average of three counts
15	Volume	V	µl	Venkataraman, Yaamini R		total of tripour with larvae
16	Larvae	Larvae	#	Venkataraman, Yaamini R		d-hinge larvae produced by each cross
17	Hatching rate	Hatching	%	Venkataraman, Yaamini R		D-Hinge in tripour from total number of eggs used
18	Treatment	Treat		Venkataraman, Yaamini R		Parental
19	Treatment	Treat		Venkataraman, Yaamini R		Female
20	Treatment	Treat		Venkataraman, Yaamini R		Male
21	Identification	ID		Venkataraman, Yaamini R		Sire
22	Alkalinity, total	AT	µmol/kg	Venkataraman, Yaamini R		Female
23	pH	pH		Venkataraman, Yaamini R		total scale, Female
24	Salinity	Sal		Venkataraman, Yaamini R		Female
25	Temperature, water	Temp	°C	Venkataraman, Yaamini R		Female
26	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
27	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
28	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
29	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
30	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
31	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
32	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
33	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
34	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Female
35	Alkalinity, total	AT	µmol/kg	Venkataraman, Yaamini R		Male
36	pH	pH		Venkataraman, Yaamini R		total scale, male
37	Salinity	Sal		Venkataraman, Yaamini R		Male
38	Temperature, water	Temp	°C	Venkataraman, Yaamini R		Male
39	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male
40	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male
41	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male
42	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male
43	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male
44	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male
45	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male
46	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male
47	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Male

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1170 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 ID (Bucket IDs for two replicates...)	6 Vol [ml] (Pool used for spawn)	7 Eggs [#] (used for spawn)	8 Larvae [#] (D-Hinge-Count-1)	9 Vol [ml] (sample used for d-hinge count)	10 Larvae [#] (D-Hinge-Count-2)	11 Vol [ml] (sample used for d-hinge count)	12 Larvae [#] (D-Hinge-Count-3)	13 Vol [ml] (sample used for d-hinge count)	14 Larvae [#] (average of three counts)	15 V [µl] (total of tripour with larvae)	16 Larvae [#] (d-hinge larvae produced by ea...)	17 Hatching [%] (D-Hinge in tripour from total...)	18 Treat (Parental)	19 Treat (Female)	20 Treat (Male)	21 ID (Sire)	22 AT [µmol/kg] (Female)	23 pH (total scale, Female)	24 Sal (Female)	25 Temp [°C] (Female)	26 CSC flag (Female)	27 CO2 [µmol/kg] (Female)	28 fCO2water_SST_wet [µatm] (Female)	29 pCO2water_SST_wet [µatm] (Female)	30 [HCO3]- [µmol/kg] (Female)	31 [CO3]2- [µmol/kg] (Female)	32 DIC [µmol/kg] (Female)	33 Omega Arg (Female)	34 Omega Cal (Female)	35 AT [µmol/kg] (Male)	36 pH (total scale, male)	37 Sal (Male)	38 Temp [°C] (Male)	39 CSC flag (Male)	40 CO2 [µmol/kg] (Male)	41 fCO2water_SST_wet [µatm] (Male)	42 pCO2water_SST_wet [µatm] (Male)	43 [HCO3]- [µmol/kg] (Male)	44 [CO3]2- [µmol/kg] (Male)	45 DIC [µmol/kg] (Male)	46 Omega Arg (Male)	47 Omega Cal (Male)
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	15-Jan	20.038	210000.000	68.0	1000	96.0	1000	103	1000	89.000	800000	71200.000	34	Low-Low	Low pH	Low pH	A	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	16-Feb	20.038	210000.000	75.0	250	57.0	250	80	250	70.667	350000	98933.333	47	Low-Low	Low pH	Low pH	B	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	18-Apr	20.038	210000.000	131.0	250	159.0	250	157	250	149.000	180000	107280.000	51	Low-Low	Low pH	Low pH	C	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	19-May	20.038	210000.000	132.0	250	72.0	250	91	250	98.333	225000	88500.000	42	Low-Low	Low pH	Low pH	D	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	20-Jun	20.038	210000.000	103.0	250	118.0	250	99	250	106.667	200000	85333.333	41	Low-Low	Low pH	Low pH	E	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	21-Jul	20.038	210000.000	66.0	210	44.0	210	95	210	68.333	200000	65079.365	31	Low-Low	Low pH	Low pH	F	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	23-Sep	20.038	210000.000	95.0	250	107.0	250	118	250	106.667	220000	93866.667	45	Low-Ambient	Low pH	Ambient	G	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	24-Oct	20.038	210000.000	88.0	250	67.0	250	97	250	84.000	200000	67200.000	32	Low-Ambient	Low pH	Ambient	H	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	25-Nov	20.038	210000.000	121.0	250	123.0	250	115	250	119.667	210000	100520.000	48	Low-Ambient	Low pH	Ambient	I	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	26-Dec	20.038	210000.000	73.0	300	42.0	300	55	300	56.667	200000	37777.778	18	Low-Ambient	Low pH	Ambient	J	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	13-27	20.038	210000.000	156.0	250	139.0	250	161	250	152.000	225000	136800.000	65	Low-Ambient	Low pH	Ambient	K	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	14-28	20.038	210000.000	107.0	250	80.0	250	112	250	99.667	200000	79733.333	38	Low-Ambient	Low pH	Ambient	L	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	29-43	16.071	210000.000	136.5	250	136.5	250	110	250	127.667	210000	107240.000	51	Ambient-Low	Ambient	Low pH	A	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	30-44	16.071	210000.000	78.0	220	92.0	220	70	220	80.000	200000	72727.273	35	Ambient-Low	Ambient	Low pH	B	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	32-46	16.071	210000.000	212.0	250	207.0	250	219	250	212.667	250000	212666.667	101	Ambient-Low	Ambient	Low pH	C	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	33-47	16.071	210000.000	132.0	250	161.0	250	124	250	139.000	220000	122320.000	58	Ambient-Low	Ambient	Low pH	D	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	34-48	16.071	210000.000	124.0	250	137.0	250	135	250	132.000	220000	116160.000	55	Ambient-Low	Ambient	Low pH	E	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	35-49	16.071	210000.000	222.0	250	302.0	250	259	250	261.000	200000	208800.000	99	Ambient-Low	Ambient	Low pH	F	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07	2686.12	7.31	27.59	10.80	8.00	135.12	3033.25	3044.88	2620.43	27.79	2783.34	0.43	0.69
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	37-51	16.071	210000.000	185.0	250	158.0	250	141	250	161.333	200000	129066.667	61	Ambient-Ambient	Ambient	Ambient	G	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	38-52	16.071	210000.000	168.0	250	155.0	250	175	250	166.000	230000	152720.000	73	Ambient-Ambient	Ambient	Ambient	H	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	39-53	16.071	210000.000	144.0	250	144.0	250	131	250	139.667	200000	111733.333	53	Ambient-Ambient	Ambient	Ambient	I	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	40-54	16.071	210000.000	128.0	250	102.0	250	110	250	113.333	200000	90666.667	43	Ambient-Ambient	Ambient	Ambient	J	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	41-55	16.071	210000.000	145.0	250	141.0	250	137	250	141.000	200000	112800.000	54	Ambient-Ambient	Ambient	Ambient	K	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	42-56	16.071	210000.000	232.0	200	286.0	200	215	200	244.333	200000	244333.333	116	Ambient-Ambient	Ambient	Ambient	L	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07	2555.27	7.82	27.62	11.34	8.00	36.69	837.88	841.07	2357.06	83.33	2477.08	1.29	2.07
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	57-59	38.043	617647.059	443.0	200	482.0	200	571	200	498.667	200000	498666.667	81	HeatShock-HeatShock	HeatShock	HeatShock	M
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	58-60	38.043	617647.059	185.0	100	173.0	100	170	100	176.000	200000	352000.000	57	HeatShock-HeatShock	HeatShock	HeatShock	N