Diehl, Nora; Karsten, Ulf; Bischof, Kai (2020): Impacts of combined temperature and salinity stress on the endemic Arctic brown seaweed Laminaria solidungula – physiological and biochemical results of laboratory experiments with young sporophytes [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.919748
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Abstract:
Macroalgae such as kelp are important ecosystem engineers in the Polar Regions and potentially affected by freshening and ocean warming. The endemic Arctic kelp Laminaria solidungula might be particularly imperiled and become locally extinct from Arctic fjord systems in the future, since temperature increase is most pronounced in the Polar Regions. Additionally, increased temperatures cause glacier and sea ice melting and enhancing terrestrial run-off from snowfields, which eventually can result in hyposaline conditions in fjord systems. We conducted a multiple-stressor experiment at four temperatures (0, 5, 10, 15 °C) and two salinities (SA 25, 35) over 14 days to investigate the combined effects of increasing temperature and decreasing salinities on young L. solidungula sporophytes. The experiment was conducted with laboratory cultures (AWI culture number 3130) at Alfred Wegener Institute - Helmholtz Centre for Polar and Marine Research, Bremerhaven in February 2018. As physiological parameter, the maximum photosynthetic quantum yield of photosystem II (Fv/Fm; Imaging-PAM) was monitored every fourth day with an Imaging-PAM (Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany). The total nitrogen, total carbon content was analyzed with an elemental analyzer and the C:N ratio calculated. Phlorotannins, mannitol as well as absolute pigment concentrations were analyzed using a HPLC and the deepoxydation state of the xanthophyll cycle (DPS) calculated.
Related to:
Diehl, Nora; Karsten, Ulf; Bischof, Kai (2020): Impacts of combined temperature and salinity stress on the endemic Arctic brown seaweed Laminaria solidungula J. Agardh. Polar Biology, 43(6), 647-656, https://doi.org/10.1007/s00300-020-02668-5
Comment:
Samples:
laboratory cultures: gametophyte strain of Laminaria solidungula from Spitsbergen – as stock culture obtained from the Alfred Wegener Institute for Polar and Marine Research, Bremerhaven, Germany; AWI culture number 3130 – not separated into male and female gametophytes
Treatment details – two-factorial experiment:
experimental set-up -> 4 temperatures, 2 salinities, 2L aerated Kautex bottles, ½ PES, 30 μmol m-2 s-1 PAR, 16:8-h-light:dark conditions, medium changed 2x/week
initial -> 0°C, total salinity (SA) 35
temperature acclimation -> within 1 week
treatments -> 0°C_SA25, 0°C_SA35, 5°C_SA25, 5°C_SA35, 10°C_SA25, 10°C_SA35, 15°C_SA25, 15°C_SA35 for 10 days
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Species | Species | Diehl, Nora | |||
| 2 | Family | Family | Diehl, Nora | |||
| 3 | Treatment: temperature | T:temp | °C | Diehl, Nora | ||
| 4 | Treatment: salinity | T:sal | Diehl, Nora | Treatment: salinity | ||
| 5 | Replicate | Repl | Diehl, Nora | |||
| 6 | Chlorophyll a | Chl a | µg/g | Diehl, Nora | High Performance Liquid Chromatography (HPLC) | |
| 7 | Chlorophyll c2 | Chl c2 | µg/g | Diehl, Nora | High Performance Liquid Chromatography (HPLC) | Chl c2 |
| 8 | Fucoxanthin | Fuco | µg/g | Diehl, Nora | High Performance Liquid Chromatography (HPLC) | |
| 9 | beta-Carotene | b-Car | µg/g | Diehl, Nora | High Performance Liquid Chromatography (HPLC) | |
| 10 | Violaxanthin + Antheraxanthin + Zeaxanthin | Viola+Anth+Zea | µg/g | Diehl, Nora | High Performance Liquid Chromatography (HPLC) | |
| 11 | De-expoxidation state of xanthophyll cycle | DPS | Diehl, Nora | DPS | ||
| 12 | Phlorotannins | Phloro | mg/g | Diehl, Nora | ||
| 13 | Mannitol | Mannitol | µmol/g | Diehl, Nora | High Performance Liquid Chromatography (HPLC) | |
| 14 | Carbon/Nitrogen ratio | C/N | mol/mol | Diehl, Nora | ||
| 15 | Carbon, per dry mass | C/dm | mg/g | Diehl, Nora | ||
| 16 | Nitrogen, per dry mass | N/dm | mg/g | Diehl, Nora | ||
| 17 | Maximum quantum yield of photosystem II | Fv/Fm | Diehl, Nora | Imaging-PAM (Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany) | day 0 | |
| 18 | Maximum quantum yield of photosystem II | Fv/Fm | Diehl, Nora | Imaging-PAM (Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany) | day 7 | |
| 19 | Maximum quantum yield of photosystem II | Fv/Fm | Diehl, Nora | Imaging-PAM (Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany) | day 11 | |
| 20 | Maximum quantum yield of photosystem II | Fv/Fm | Diehl, Nora | Imaging-PAM (Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany) | day 15 | |
| 21 | Maximum quantum yield of photosystem II | Fv/Fm | Diehl, Nora | Imaging-PAM (Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany) | day 19 | |
| 22 | Maximum quantum yield of photosystem II | Fv/Fm | Diehl, Nora | Imaging-PAM (Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany) | day 22 |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
2212 data points
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