Bommarito, Claudia; Pansch, Christian; Khosravi, Maral; Pranovi, Fabio; Wahl, Martin; Thieltges, David W (2020): Exp. II: cercarial activity and survival [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.914100, In: Bommarito, C et al. (2020): Experiments on the life cycle of the trematode Himasthla elongata [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.914102
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Abstract:
For cercarial activity and survival infected periwinkles were acclimated for three weeks under the different treatments (n=10 individuals per treatment) and incubated to allow for cercarial release. At the start of the experiment, approximately 45 cercariae per treatment were added individually to wells of three 96-well plates The 96-well plates were then exposed to the different temperature treatments placing them in the six thermobaths (three 96-well plates per treatment combination). Activity and survival were then assessed under a stereomicroscope after 4, 6, 9, 18, 27, and 45 hours. Cercariae were considered fully active when they were constantly swirling around (category: fully active). When laying at the bottom and not reacting, these were considered dead (category: dead), when still reacting, even if slowly, these were still considered alive (category: alive). This last category included "fully active".
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Species | Species | Bommarito, Claudia | |||
| 2 | Identification | ID | Bommarito, Claudia | well-plate | ||
| 3 | Identification | ID | Bommarito, Claudia | cercaria number | ||
| 4 | Treatment: temperature | T:temp | °C | Bommarito, Claudia | ||
| 5 | Treatment | Treat | Bommarito, Claudia | Salinity PSU | ||
| 6 | Time in hours | Time | h | Bommarito, Claudia | ||
| 7 | Variable | Variable | Bommarito, Claudia | |||
| 8 | Status | Status | Bommarito, Claudia | activity 1=not fully active anymore (at the indicated time); survival 1= dead, 0= not dead yet |
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Creative Commons Attribution 4.0 International (CC-BY-4.0)
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