Citation:

PANGAEA.914102

Name: Exp. II: cercarial activity and survival
Published: 2020-03-26
License: https://creativecommons.org/licenses/by/4.0/
Keywords: infectivity; Littorina littorea; Mytilus galloprovincialis; Salinity; trematode

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Abstract:

For cercarial activity and survival infected periwinkles were acclimated for three weeks under the different treatments (n=10 individuals per treatment) and incubated to allow for cercarial release. At the start of the experiment, approximately 45 cercariae per treatment were added individually to wells of three 96-well plates The 96-well plates were then exposed to the different temperature treatments placing them in the six thermobaths (three 96-well plates per treatment combination). Activity and survival were then assessed under a stereomicroscope after 4, 6, 9, 18, 27, and 45 hours. Cercariae were considered fully active when they were constantly swirling around (category: fully active). When laying at the bottom and not reacting, these were considered dead (category: dead), when still reacting, even if slowly, these were still considered alive (category: alive). This last category included "fully active".

Keyword(s):

infectivity; Littorina littorea; Mytilus galloprovincialis; Salinity; trematode

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Comment
1	Species	Species		Bommarito, Claudia
2	Identification	ID		Bommarito, Claudia	well-plate
3	Identification	ID		Bommarito, Claudia	cercaria number
4	Treatment: temperature	T:temp	°C	Bommarito, Claudia
5	Treatment	Treat		Bommarito, Claudia	Salinity PSU
6	Time in hours	Time	h	Bommarito, Claudia
7	Variable	Variable		Bommarito, Claudia
8	Status	Status		Bommarito, Claudia	activity 1=not fully active anymore (at the indicated time); survival 1= dead, 0= not dead yet

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Size:

2600 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 ID (well-plate)	3 ID (cercaria number)	4 T:temp [°C]	5 Treat (Salinity PSU)	6 Time [h]	7 Variable	8 Status (activity 1=not fully active a...)
Himasthla elongata	2	cerc 1	19	13	27	activity	1
Himasthla elongata	2	cerc 2	19	13	27	activity	1
Himasthla elongata	2	cerc 3	19	13	6	activity	1
Himasthla elongata	2	cerc 4	19	13	6	activity	1
Himasthla elongata	2	cerc 5	19	13	27	activity	1
Himasthla elongata	2	cerc 6	19	13	18	activity	1
Himasthla elongata	2	cerc 7	19	13	6	activity	1
Himasthla elongata	2	cerc 8	19	13	6	activity	1
Himasthla elongata	2	cerc 9	19	13	6	activity	1
Himasthla elongata	1	cerc 10	19	13	2	activity	1
Himasthla elongata	1	cerc 11	19	13	6	activity	1
Himasthla elongata	1	cerc 12	19	13	27	activity	1
Himasthla elongata	1	cerc 13	19	13	27	activity	1
Himasthla elongata	1	cerc 14	19	13	6	activity	1
Himasthla elongata	1	cerc 15	19	13	27	activity	1
Himasthla elongata	1	cerc 16	19	13	27	activity	1
Himasthla elongata	1	cerc 17	19	13	27	activity	1
Himasthla elongata	1	cerc 18	19	13	27	activity	1
Himasthla elongata	1	cerc 19	19	13	27	activity	1
Himasthla elongata	1	cerc 20	19	13	6	activity	1
Himasthla elongata	1	cerc 21	19	13	6	activity	1
Himasthla elongata	3	cerc 22	19	13	2	activity	1
Himasthla elongata	3	cerc 23	19	13	9	activity	1
Himasthla elongata	3	cerc 24	19	13	18	activity	1
Himasthla elongata	3	cerc 25	19	13	27	activity	1
Himasthla elongata	3	cerc 26	19	13	18	activity	1
Himasthla elongata	3	cerc 27	19	13	27	activity	1
Himasthla elongata	3	cerc 28	19	13	6	activity	1
Himasthla elongata	3	cerc 29	19	13	27	activity	1
Himasthla elongata	3	cerc 30	19	13	6	activity	1
Himasthla elongata	1	cerc 31	19	16	6	activity	1
Himasthla elongata	1	cerc 32	19	16	4	activity	1
Himasthla elongata	1	cerc 33	19	16	4	activity	1
Himasthla elongata	1	cerc 34	19	16	27	activity	1
Himasthla elongata	1	cerc 35	19	16	18	activity	1
Himasthla elongata	1	cerc 36	19	16	9	activity	1
Himasthla elongata	1	cerc 37	19	16	9	activity	1
Himasthla elongata	1	cerc 38	19	16	2	activity	1
Himasthla elongata	2	cerc 39	19	16	6	activity	1
Himasthla elongata	2	cerc 40	19	16	6	activity	1
Himasthla elongata	2	cerc 41	19	16	6	activity	1
Himasthla elongata	2	cerc 42	19	16	6	activity	1
Himasthla elongata	2	cerc 43	19	16	27	activity	1
Himasthla elongata	2	cerc 44	19	16	27	activity	1
Himasthla elongata	2	cerc 45	19	16	9	activity	1
Himasthla elongata	2	cerc 46	19	16	9	activity	1
Himasthla elongata	2	cerc 47	19	16	6	activity	1
Himasthla elongata	2	cerc 48	19	16	27	activity	1
Himasthla elongata	3	cerc 49	19	16	2	activity	1
Himasthla elongata	3	cerc 50	19	16	6	activity	1
Himasthla elongata	3	cerc 51	19	16	27	activity	1
Himasthla elongata	3	cerc 52	19	16	4	activity	1
Himasthla elongata	3	cerc 53	19	16	6	activity	1
Himasthla elongata	3	cerc 54	19	16	27	activity	1
Himasthla elongata	3	cerc 55	19	16	27	activity	1
Himasthla elongata	3	cerc 56	19	16	27	activity	1
Himasthla elongata	3	cerc 57	19	16	27	activity	1
Himasthla elongata	3	cerc 58	19	16	27	activity	1
Himasthla elongata	3	cerc 59	19	16	9	activity	1
Himasthla elongata	1	cerc 60	19	19	27	activity	1
Himasthla elongata	1	cerc 61	19	19	27	activity	1
Himasthla elongata	1	cerc 62	19	19	45	activity	1
Himasthla elongata	1	cerc 63	19	19	45	activity	1
Himasthla elongata	1	cerc 64	19	19	18	activity	1
Himasthla elongata	1	cerc 65	19	19	18	activity	1
Himasthla elongata	1	cerc 66	19	19	27	activity	1
Himasthla elongata	1	cerc 67	19	19	18	activity	1
Himasthla elongata	1	cerc 68	19	19	9	activity	1
Himasthla elongata	2	cerc 69	19	19	9	activity	1
Himasthla elongata	2	cerc 70	19	19	27	activity	1
Himasthla elongata	2	cerc 71	19	19	27	activity	1
Himasthla elongata	2	cerc 72	19	19	6	activity	1
Himasthla elongata	2	cerc 73	19	19	27	activity	1
Himasthla elongata	2	cerc 74	19	19	27	activity	1
Himasthla elongata	2	cerc 75	19	19	18	activity	1
Himasthla elongata	3	cerc 76	19	19	27	activity	1
Himasthla elongata	3	cerc 77	19	19	18	activity	1
Himasthla elongata	3	cerc 78	19	19	45	activity	1
Himasthla elongata	3	cerc 79	19	19	9	activity	1
Himasthla elongata	3	cerc 80	19	19	18	activity	1
Himasthla elongata	3	cerc 81	19	19	45	activity	1
Himasthla elongata	3	cerc 82	19	19	45	activity	1
Himasthla elongata	3	cerc 83	23	13	4	activity	1
Himasthla elongata	3	cerc 84	23	13	9	activity	1
Himasthla elongata	3	cerc 85	23	13	9	activity	1
Himasthla elongata	3	cerc 86	23	13	4	activity	1
Himasthla elongata	3	cerc 87	23	13	9	activity	1
Himasthla elongata	3	cerc 88	23	13	9	activity	1
Himasthla elongata	3	cerc 89	23	13	9	activity	1
Himasthla elongata	2	cerc 90	23	13	6	activity	1
Himasthla elongata	2	cerc 91	23	13	6	activity	1
Himasthla elongata	2	cerc 92	23	13	27	activity	1
Himasthla elongata	2	cerc 93	23	13	6	activity	1
Himasthla elongata	2	cerc 94	23	13	6	activity	1
Himasthla elongata	2	cerc 95	23	13	9	activity	1
Himasthla elongata	2	cerc 96	23	13	6	activity	1
Himasthla elongata	2	cerc 97	23	13	9	activity	1
Himasthla elongata	2	cerc 98	23	13	9	activity	1
Himasthla elongata	2	cerc 99	23	13	9	activity	1
Himasthla elongata	1	cerc 100	23	13	6	activity	1
Himasthla elongata	1	cerc 101	23	13	9	activity	1
Himasthla elongata	1	cerc 102	23	13	6	activity	1
Himasthla elongata	1	cerc 103	23	13	6	activity	1
Himasthla elongata	1	cerc 104	23	13	9	activity	1
Himasthla elongata	1	cerc 105	23	13	27	activity	1
Himasthla elongata	1	cerc 106	23	13	45	activity	1
Himasthla elongata	1	cerc 107	23	13	27	activity	1
Himasthla elongata	1	cerc 108	23	13	27	activity	1
Himasthla elongata	1	cerc 109	23	13	27	activity	1
Himasthla elongata	1	cerc 110	23	13	9	activity	1
Himasthla elongata	1	cerc 111	23	13	45	activity	1
Himasthla elongata	1	cerc 112	23	13	6	activity	1
Himasthla elongata	1	cerc 113	23	13	9	activity	1
Himasthla elongata	1	cerc 114	23	13	6	activity	1
Himasthla elongata	3	cerc 115	23	16	18	activity	1
Himasthla elongata	3	cerc 116	23	16	6	activity	1
Himasthla elongata	3	cerc 117	23	16	2	activity	1
Himasthla elongata	3	cerc 118	23	16	18	activity	1
Himasthla elongata	3	cerc 119	23	16	18	activity	1
Himasthla elongata	3	cerc 120	23	16	45	activity	1
Himasthla elongata	3	cerc 121	23	16	27	activity	1
Himasthla elongata	1	cerc 122	23	16	9	activity	1
Himasthla elongata	1	cerc 123	23	16	6	activity	1
Himasthla elongata	1	cerc 124	23	16	4	activity	1
Himasthla elongata	1	cerc 125	23	16	9	activity	1
Himasthla elongata	1	cerc 126	23	16	9	activity	1
Himasthla elongata	1	cerc 127	23	16	27	activity	1
Himasthla elongata	1	cerc 128	23	16	27	activity	1
Himasthla elongata	1	cerc 129	23	16	27	activity	1
Himasthla elongata	1	cerc 130	23	16	9	activity	1
Himasthla elongata	2	cerc 131	23	16	18	activity	1
Himasthla elongata	2	cerc 132	23	16	4	activity	1
Himasthla elongata	2	cerc 133	23	16	6	activity	1
Himasthla elongata	2	cerc 134	23	16	27	activity	1
Himasthla elongata	2	cerc 135	23	16	18	activity	1
Himasthla elongata	2	cerc 136	23	16	27	activity	1
Himasthla elongata	1	cerc 137	23	19	6	activity	1
Himasthla elongata	1	cerc 138	23	19	27	activity	1
Himasthla elongata	1	cerc 139	23	19	18	activity	1
Himasthla elongata	1	cerc 140	23	19	18	activity	1
Himasthla elongata	1	cerc 141	23	19	18	activity	1
Himasthla elongata	1	cerc 142	23	19	27	activity	1
Himasthla elongata	1	cerc 143	23	19	18	activity	1
Himasthla elongata	1	cerc 144	23	19	9	activity	1
Himasthla elongata	1	cerc 145	23	19	27	activity	1
Himasthla elongata	2	cerc 146	23	19	27	activity	1
Himasthla elongata	2	cerc 147	23	19	18	activity	1
Himasthla elongata	2	cerc 148	23	19	27	activity	1
Himasthla elongata	2	cerc 149	23	19	18	activity	1
Himasthla elongata	2	cerc 150	23	19	4	activity	1
Himasthla elongata	2	cerc 151	23	19	18	activity	1
Himasthla elongata	2	cerc 152	23	19	6	activity	1
Himasthla elongata	2	cerc 153	23	19	18	activity	1
Himasthla elongata	2	cerc 154	23	19	6	activity	1
Himasthla elongata	2	cerc 155	23	19	27	activity	1
Himasthla elongata	2	cerc 156	23	19	27	activity	1
Himasthla elongata	2	cerc 157	23	19	18	activity	1
Himasthla elongata	2	cerc 158	23	19	18	activity	1
Himasthla elongata	3	cerc 159	23	19	45	activity	1
Himasthla elongata	3	cerc 160	23	19	18	activity	1
Himasthla elongata	3	cerc 161	23	19	6	activity	1
Himasthla elongata	3	cerc 162	23	19	45	activity	1
Himasthla elongata	2	1	19	13	45	Survival	1
Himasthla elongata	2	2	19	13	45	Survival	1
Himasthla elongata	2	3	19	13	45	Survival	1
Himasthla elongata	2	4	19	13	45	Survival	1
Himasthla elongata	2	5	19	13	45	Survival	1
Himasthla elongata	2	6	19	13	27	Survival	1
Himasthla elongata	2	7	19	13	45	Survival	1
Himasthla elongata	2	8	19	13	45	Survival	1
Himasthla elongata	2	9	19	13	45	Survival	1
Himasthla elongata	1	10	19	13	45	Survival	1
Himasthla elongata	1	11	19	13	27	Survival	1
Himasthla elongata	1	12	19	13	45	Survival	1
Himasthla elongata	1	13	19	13	45	Survival	1
Himasthla elongata	1	14	19	13	45	Survival	1
Himasthla elongata	1	15	19	13	45	Survival	1
Himasthla elongata	1	16	19	13	45	Survival	1
Himasthla elongata	1	17	19	13	45	Survival	0
Himasthla elongata	1	18	19	13	27	Survival	1
Himasthla elongata	1	19	19	13	45	Survival	0
Himasthla elongata	1	20	19	13	45	Survival	1
Himasthla elongata	1	21	19	13	45	Survival	0
Himasthla elongata	3	22	19	13	45	Survival	0
Himasthla elongata	3	23	19	13	45	Survival	0
Himasthla elongata	3	24	19	13	45	Survival	1
Himasthla elongata	3	25	19	13	45	Survival	1
Himasthla elongata	3	26	19	13	45	Survival	1
Himasthla elongata	3	27	19	13	45	Survival	0
Himasthla elongata	3	28	19	13	27	Survival	1
Himasthla elongata	3	29	19	13	45	Survival	1
Himasthla elongata	3	30	19	13	45	Survival	1
Himasthla elongata	1	1	19	16	45	Survival	1
Himasthla elongata	1	2	19	16	45	Survival	0
Himasthla elongata	1	3	19	16	45	Survival	1
Himasthla elongata	1	4	19	16	45	Survival	1
Himasthla elongata	1	5	19	16	45	Survival	1
Himasthla elongata	1	6	19	16	45	Survival	1
Himasthla elongata	1	7	19	16	45	Survival	1
Himasthla elongata	1	8	19	16	2	Survival	1
Himasthla elongata	2	9	19	16	45	Survival	1
Himasthla elongata	2	10	19	16	45	Survival	0
Himasthla elongata	2	11	19	16	45	Survival	0
Himasthla elongata	2	12	19	16	45	Survival	1
Himasthla elongata	2	13	19	16	45	Survival	1
Himasthla elongata	2	14	19	16	45	Survival	1
Himasthla elongata	2	15	19	16	27	Survival	1
Himasthla elongata	2	16	19	16	45	Survival	1
Himasthla elongata	2	17	19	16	45	Survival	1
Himasthla elongata	2	18	19	16	45	Survival	1
Himasthla elongata	3	19	19	16	45	Survival	1
Himasthla elongata	3	20	19	16	45	Survival	0
Himasthla elongata	3	21	19	16	45	Survival	1
Himasthla elongata	3	22	19	16	45	Survival	1
Himasthla elongata	3	23	19	16	45	Survival	0
Himasthla elongata	3	24	19	16	45	Survival	0
Himasthla elongata	3	25	19	16	45	Survival	0
Himasthla elongata	3	26	19	16	45	Survival	1
Himasthla elongata	3	27	19	16	45	Survival	0
Himasthla elongata	3	28	19	16	45	Survival	1
Himasthla elongata	3	29	19	16	45	Survival	0
Himasthla elongata	1	1	19	19	45	Survival	1
Himasthla elongata	1	2	19	19	45	Survival	1
Himasthla elongata	1	3	19	19	45	Survival	1
Himasthla elongata	1	4	19	19	45	Survival	1
Himasthla elongata	1	5	19	19	45	Survival	1
Himasthla elongata	1	6	19	19	45	Survival	1
Himasthla elongata	1	7	19	19	45	Survival	0
Himasthla elongata	1	8	19	19	45	Survival	0
Himasthla elongata	1	9	19	19	45	Survival	1
Himasthla elongata	2	10	19	19	27	Survival	1
Himasthla elongata	2	11	19	19	45	Survival	1
Himasthla elongata	2	12	19	19	45	Survival	1
Himasthla elongata	2	13	19	19	45	Survival	1
Himasthla elongata	2	14	19	19	45	Survival	1
Himasthla elongata	2	15	19	19	45	Survival	1
Himasthla elongata	2	16	19	19	45	Survival	0
Himasthla elongata	3	17	19	19	45	Survival	0
Himasthla elongata	3	18	19	19	45	Survival	0
Himasthla elongata	3	19	19	19	45	Survival	0
Himasthla elongata	3	20	19	19	27	Survival	1
Himasthla elongata	3	21	19	19	45	Survival	1
Himasthla elongata	3	22	19	19	45	Survival	1
Himasthla elongata	3	23	19	19	45	Survival	1
Himasthla elongata	3	1	23	13	27	Survival	1
Himasthla elongata	3	2	23	13	45	Survival	1
Himasthla elongata	3	3	23	13	45	Survival	1
Himasthla elongata	3	4	23	13	18	Survival	1
Himasthla elongata	3	5	23	13	45	Survival	0
Himasthla elongata	3	6	23	13	45	Survival	0
Himasthla elongata	3	7	23	13	45	Survival	1
Himasthla elongata	2	8	23	13	45	Survival	1
Himasthla elongata	2	9	23	13	27	Survival	1
Himasthla elongata	2	10	23	13	45	Survival	0
Himasthla elongata	2	11	23	13	45	Survival	1
Himasthla elongata	2	12	23	13	27	Survival	1
Himasthla elongata	2	13	23	13	45	Survival	1
Himasthla elongata	2	14	23	13	27	Survival	1
Himasthla elongata	2	15	23	13	45	Survival	1
Himasthla elongata	2	16	23	13	27	Survival	1
Himasthla elongata	2	17	23	13	45	Survival	0
Himasthla elongata	1	18	23	13	45	Survival	1
Himasthla elongata	1	19	23	13	45	Survival	1
Himasthla elongata	1	20	23	13	27	Survival	1
Himasthla elongata	1	21	23	13	27	Survival	1
Himasthla elongata	1	22	23	13	27	Survival	1
Himasthla elongata	1	23	23	13	45	Survival	1
Himasthla elongata	1	24	23	13	45	Survival	1
Himasthla elongata	1	25	23	13	45	Survival	1
Himasthla elongata	1	26	23	13	27	Survival	1
Himasthla elongata	1	27	23	13	45	Survival	1
Himasthla elongata	1	28	23	13	27	Survival	1
Himasthla elongata	1	29	23	13	45	Survival	1
Himasthla elongata	1	30	23	13	27	Survival	1
Himasthla elongata	1	31	23	13	45	Survival	1
Himasthla elongata	1	32	23	13	45	Survival	1
Himasthla elongata	3	1	23	16	18	Survival	1
Himasthla elongata	3	2	23	16	27	Survival	1
Himasthla elongata	3	3	23	16	27	Survival	1
Himasthla elongata	3	4	23	16	27	Survival	1
Himasthla elongata	3	5	23	16	45	Survival	1
Himasthla elongata	3	6	23	16	45	Survival	1
Himasthla elongata	3	7	23	16	45	Survival	1
Himasthla elongata	1	8	23	16	45	Survival	1
Himasthla elongata	1	9	23	16	45	Survival	1
Himasthla elongata	1	10	23	16	27	Survival	1
Himasthla elongata	1	11	23	16	27	Survival	1
Himasthla elongata	1	12	23	16	45	Survival	1
Himasthla elongata	1	13	23	16	27	Survival	1
Himasthla elongata	1	14	23	16	45	Survival	1
Himasthla elongata	1	15	23	16	27	Survival	1
Himasthla elongata	1	16	23	16	45	Survival	1
Himasthla elongata	2	17	23	16	45	Survival	1
Himasthla elongata	2	18	23	16	27	Survival	1
Himasthla elongata	2	19	23	16	27	Survival	1
Himasthla elongata	2	20	23	16	27	Survival	1
Himasthla elongata	2	21	23	16	45	Survival	1
Himasthla elongata	2	22	23	16	45	Survival	1
Himasthla elongata	1	23	23	16	27	Survival	1
Himasthla elongata	1	1	23	19	45	Survival	1
Himasthla elongata	1	2	23	19	45	Survival	0
Himasthla elongata	1	3	23	19	18	Survival	1
Himasthla elongata	1	4	23	19	27	Survival	1
Himasthla elongata	1	5	23	19	45	Survival	1
Himasthla elongata	1	6	23	19	45	Survival	1
Himasthla elongata	1	7	23	19	45	Survival	1
Himasthla elongata	1	8	23	19	27	Survival	1
Himasthla elongata	2	9	23	19	45	Survival	1
Himasthla elongata	2	10	23	19	45	Survival	1
Himasthla elongata	2	11	23	19	18	Survival	1
Himasthla elongata	2	12	23	19	45	Survival	1
Himasthla elongata	2	13	23	19	45	Survival	1
Himasthla elongata	2	14	23	19	45	Survival	1
Himasthla elongata	2	15	23	19	45	Survival	0
Himasthla elongata	2	16	23	19	45	Survival	0
Himasthla elongata	2	17	23	19	27	Survival	1
Himasthla elongata	2	18	23	19	27	Survival	1
Himasthla elongata	2	19	23	19	45	Survival	1
Himasthla elongata	2	20	23	19	45	Survival	1
Himasthla elongata	2	21	23	19	45	Survival	1
Himasthla elongata	3	22	23	19	18	Survival	1
Himasthla elongata	3	23	23	19	45	Survival	1
Himasthla elongata	3	24	23	19	27	Survival	1
Himasthla elongata	3	25	23	19	45	Survival	1
Himasthla elongata	3	26	23	19	45	Survival	1