Busch, Kathrin; Wurz, Erik; Rapp, Hans Tore; Bayer, Kristina; Franke, Andre; Hentschel, Ute (2020): Metadata and NCBI-Accession numbers of 16S data for seawater and Craniella sponges from the North Atlantic Ocean and an aquarium experiment in 2017 and 2018 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.913890
Always quote citation above when using data! You can download the citation in several formats below.
Abstract:
Deep-sea sponge grounds are underexplored ecosystems that provide numerous goods and services to the functioning of the deep-sea. This study assessed the microbial diversity (by 16S rRNA gene amplicon sequencing) in embryos, juveniles and adults of Craniella zetlandica and Craniella infrequens, common and abundant representatives of deep-sea sponge grounds in the North Atlantic. For this study, in total 39 sponge individuals of the two sponge species were collected and analysed for their associated microbial community composition: C. zetlandica (8 adults, of which one was brooding, and 9 juveniles) and C. infrequens (8 adults, of which four were brooding, and 9 juveniles). We use the term 'juvenile' for small (i.e. mean diameter = 1 cm for C. infrequens; and maximal diameter = 0.3 cm for C. zetlandica), young individuals. For C. zetlandica, juveniles were sampled in September 2018 from an aquarium system (Bergen, Norway). C. zetlandica juveniles were smaller (and most likely younger) than the in situ sampled C. infrequens juveniles and therefore flash-frozen as a whole. Embryos of both sponge species were carefully picked out of the parent sponges with sterile spring steel forceps onboard the research vessel using a stereomicroscope. Ten whole embryos were pooled per adult sponge to account for the small biomass. Data such as presented here provide information on the recruitment of deep-sea sponge holobionts which is needed to develop integrated management tools of such vulnerable marine ecosystems.
Keyword(s):
Supplement to:
Busch, Kathrin; Wurz, Erik; Rapp, Hans Tore; Bayer, Kristina; Franke, Andre; Hentschel, Ute (2020): Chloroflexi Dominate the Deep-Sea Golf Ball Sponges Craniella zetlandica and Craniella infrequens Throughout Different Life Stages. Frontiers in Marine Science, 7, https://doi.org/10.3389/fmars.2020.00674
Related to:
Busch, Kathrin; Wurz, Erik; Rapp, Hans Tore; Bayer, Kristina; Franke, Andre; Hentschel, Ute (2020): In situ footage of a spawning deep-sea golf ball sponge Craniella zetlandica. PANGAEA, https://doi.org/10.1594/PANGAEA.918218
Project(s):
Funding:
Horizon 2020 (H2020), grant/award no. 679849: Deep-sea Sponge Grounds Ecosystems of the North Atlantic
Coverage:
Median Latitude: 72.719640 * Median Longitude: 12.170438 * South-bound Latitude: 70.262200 * West-bound Longitude: 7.398600 * North-bound Latitude: 73.836900 * East-bound Longitude: 22.481400
Date/Time Start: 2017-07-27T00:47:52 * Date/Time End: 2018-09-19T00:00:00
Minimum DEPTH, water: 217 m * Maximum DEPTH, water: 1070 m
Event(s):
GS2017110-15-CTD-05 * Latitude Start: 73.800000 * Longitude Start: 7.399000 * Latitude End: 73.800000 * Longitude End: 7.399000 * Date/Time Start: 2017-07-27T00:48:00 * Date/Time End: 2017-07-27T01:25:00 * Elevation Start: -1083.0 m * Elevation End: -1083.0 m * Location: Schultz Bank * Campaign: GS2017110 * Basis: G. O. Sars (2003) * Method/Device: CTD/Rosette (CTD-RO) * Comment: Samples for SPOM, NUTRIENTS, Si, Microbial DNA and microbial counts
GS2017110-19-ROV10 * Latitude Start: 73.814220 * Longitude Start: 7.444673 * Latitude End: 73.814700 * Longitude End: 7.445200 * Date/Time Start: 2017-07-28T03:26:00 * Date/Time End: 2017-07-28T11:37:00 * Elevation: -894.0 m * Location: Schultz Bank * Campaign: GS2017110 * Basis: G. O. Sars (2003) * Method/Device: Remote operated vehicle (ROV) * Comment: Deployment of chambers. Video survey of NW ridge. Rich ground on top (almost 900 m)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Busch, Kathrin | |||
| 2 | DATE/TIME | Date/Time | Busch, Kathrin | Geocode | ||
| 3 | LATITUDE | Latitude | Busch, Kathrin | Geocode | ||
| 4 | LONGITUDE | Longitude | Busch, Kathrin | Geocode | ||
| 5 | Device type | Device | Busch, Kathrin | |||
| 6 | Sample code/label | Sample label | Busch, Kathrin | |||
| 7 | Sample type | Samp type | Busch, Kathrin | |||
| 8 | Sample position | Samp pos | Busch, Kathrin | sample place | ||
| 9 | Identification | ID | Busch, Kathrin | |||
| 10 | Accession number, genetics | Access no gen | Busch, Kathrin | NCBI Accession number | ||
| 11 | Area/locality | Area | Busch, Kathrin | |||
| 12 | DEPTH, water | Depth water | m | Busch, Kathrin | Geocode | |
| 13 | Campaign | Campaign | Busch, Kathrin | |||
| 14 | Well-known text | WKT | Busch, Kathrin |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
441 data points