Citation:

PANGAEA.913890

Name: Metadata and NCBI-Accession numbers of 16S data for seawater and Craniella sponges from the North Atlantic Ocean and an aquarium experiment in 2017 and 2018
Published: 2020-03-24
License: https://creativecommons.org/licenses/by/4.0/
Keywords: amplicon sequencing; Chloroflexi; Craniella; early life stages; fluorescence in situ hybridisation; symbiosis; vulnerable marine ecosystems

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Abstract:

Deep-sea sponge grounds are underexplored ecosystems that provide numerous goods and services to the functioning of the deep-sea. This study assessed the microbial diversity (by 16S rRNA gene amplicon sequencing) in embryos, juveniles and adults of Craniella zetlandica and Craniella infrequens, common and abundant representatives of deep-sea sponge grounds in the North Atlantic. For this study, in total 39 sponge individuals of the two sponge species were collected and analysed for their associated microbial community composition: C. zetlandica (8 adults, of which one was brooding, and 9 juveniles) and C. infrequens (8 adults, of which four were brooding, and 9 juveniles). We use the term 'juvenile' for small (i.e. mean diameter = 1 cm for C. infrequens; and maximal diameter = 0.3 cm for C. zetlandica), young individuals. For C. zetlandica, juveniles were sampled in September 2018 from an aquarium system (Bergen, Norway). C. zetlandica juveniles were smaller (and most likely younger) than the in situ sampled C. infrequens juveniles and therefore flash-frozen as a whole. Embryos of both sponge species were carefully picked out of the parent sponges with sterile spring steel forceps onboard the research vessel using a stereomicroscope. Ten whole embryos were pooled per adult sponge to account for the small biomass. Data such as presented here provide information on the recruitment of deep-sea sponge holobionts which is needed to develop integrated management tools of such vulnerable marine ecosystems.

Keyword(s):

amplicon sequencing; Chloroflexi; Craniella; early life stages; fluorescence in situ hybridisation; symbiosis; vulnerable marine ecosystems

Supplement to:

Busch, Kathrin; Wurz, Erik; Rapp, Hans Tore; Bayer, Kristina; Franke, Andre; Hentschel, Ute (2020): Chloroflexi Dominate the Deep-Sea Golf Ball Sponges Craniella zetlandica and Craniella infrequens Throughout Different Life Stages. Frontiers in Marine Science, 7, https://doi.org/10.3389/fmars.2020.00674

Related to:

Busch, Kathrin; Wurz, Erik; Rapp, Hans Tore; Bayer, Kristina; Franke, Andre; Hentschel, Ute (2020): In situ footage of a spawning deep-sea golf ball sponge Craniella zetlandica. PANGAEA, https://doi.org/10.1594/PANGAEA.918218

Project(s):

Deep-sea Sponge Grounds Ecosystems of the North Atlantic (SponGES)

Funding:

Horizon 2020 (H2020), grant/award no. 679849 : Deep-sea Sponge Grounds Ecosystems of the North Atlantic

Coverage:

Median Latitude: 72.719640 * Median Longitude: 12.170438 * South-bound Latitude: 70.262200 * West-bound Longitude: 7.398600 * North-bound Latitude: 73.836900 * East-bound Longitude: 22.481400

Date/Time Start: 2017-07-27T00:47:52 * Date/Time End: 2018-09-19T00:00:00

Minimum DEPTH, water: 217 m * Maximum DEPTH, water: 1070 m

Event(s):

AQUARIUM_C.zetlandica * Date/Time: 2018-09-19T00:00:00 * Method/Device: Experiment (EXP)

GS2017110-15-CTD-05 * Latitude Start: 73.800000 * Longitude Start: 7.399000 * Latitude End: 73.800000 * Longitude End: 7.399000 * Date/Time Start: 2017-07-27T00:48:00 * Date/Time End: 2017-07-27T01:25:00 * Elevation Start: -1083.0 m * Elevation End: -1083.0 m * Location: Schultz Bank * Campaign: * Basis: * Method/Device: (CTD-RO) * Comment: Samples for SPOM, NUTRIENTS, Si, Microbial DNA and microbial counts

GS2017110-19-ROV10 * Latitude Start: 73.814220 * Longitude Start: 7.444673 * Latitude End: 73.814700 * Longitude End: 7.445200 * Date/Time Start: 2017-07-28T03:26:00 * Date/Time End: 2017-07-28T11:37:00 * Elevation: -894.0 m * Location: Schultz Bank * Campaign: * Basis: * Method/Device: Remote operated vehicle (ROV) * Comment: Deployment of chambers. Video survey of NW ridge. Rich ground on top (almost 900 m)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Comment
1	Event label	Event		Busch, Kathrin
2	DATE/TIME	Date/Time		Busch, Kathrin	Geocode
3	LATITUDE	Latitude		Busch, Kathrin	Geocode
4	LONGITUDE	Longitude		Busch, Kathrin	Geocode
5	Device type	Device		Busch, Kathrin
6	Sample code/label	Sample label		Busch, Kathrin
7	Sample type	Samp type		Busch, Kathrin
8	Sample position	Samp pos		Busch, Kathrin	sample place
9	Identification	ID		Busch, Kathrin
10	Accession number, genetics	Access no gen		Busch, Kathrin	NCBI Accession number
11	Area/locality	Area		Busch, Kathrin
12	DEPTH, water	Depth water	m	Busch, Kathrin	Geocode
13	Campaign	Campaign		Busch, Kathrin
14	Well-known text	WKT		Busch, Kathrin

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Size:

441 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 Date/Time	3 Latitude	4 Longitude	5 Device	6 Sample label	7 Samp type	8 Samp pos	9 ID	10 Access no gen	11 Area	12 Depth water [m]	13 Campaign	14 WKT
GS2017110-19-ROV10	2017-07-28T07:31	73.8193	7.4177	Remotely operated vehicle (ROV)	GS2017110-19-ROV10#12_embryos	Craniella_infrequens_embryo	in_situ	WB_00464_embryos	insdc:SRR11306491	Schulz Bank (MRGID:5039)	897	GOSars2017	LINESTRING(7.4447 73.8142, 7.4452 73.8147)
GS2017110-19-ROV10	2017-07-28T07:31	73.8193	7.4177	Remotely operated vehicle (ROV)	GS2017110-19-ROV10#12	Craniella_infrequens_adult	in_situ	WB_00464	insdc:SRR11306490	Schulz Bank (MRGID:5039)	897	GOSars2017	LINESTRING(7.4447 73.8142, 7.4452 73.8147)
GS2017110-19-ROV10	2017-07-28T07:31	73.8193	7.4177	Remotely operated vehicle (ROV)	GS2017110-19-ROV10#13_embryos	Craniella_infrequens_embryo	in_situ	WB_00465_embryos	insdc:SRR11306479	Schulz Bank (MRGID:5039)	897	GOSars2017	LINESTRING(7.4447 73.8142, 7.4452 73.8147)
GS2017110-19-ROV10	2017-07-28T07:31	73.8193	7.4177	Remotely operated vehicle (ROV)	GS2017110-19-ROV10#13	Craniella_infrequens_adult	in_situ	WB_00465	insdc:SRR11306468	Schulz Bank (MRGID:5039)	897	GOSars2017	LINESTRING(7.4447 73.8142, 7.4452 73.8147)
GS2017110-19-ROV10	2017-07-28T07:31	73.8193	7.4177	Remotely operated vehicle (ROV)	GS2017110-19-ROV10#14	Craniella_infrequens_adult	in_situ	WB_00466	insdc:SRR11306511	Schulz Bank (MRGID:5039)	897	GOSars2017	LINESTRING(7.4447 73.8142, 7.4452 73.8147)
GS2017110-19-ROV10	2017-07-28T07:31	73.8193	7.4177	Remotely operated vehicle (ROV)	GS2017110-19-ROV10#15_embryos	Craniella_infrequens_embryo	in_situ	WB_00467_embryos	insdc:SRR11306500	Schulz Bank (MRGID:5039)	897	GOSars2017	LINESTRING(7.4447 73.8142, 7.4452 73.8147)
GS2017110-19-ROV10	2017-07-28T07:31	73.8193	7.4177	Remotely operated vehicle (ROV)	GS2017110-19-ROV10#15	Craniella_infrequens_adult	in_situ	WB_00467	insdc:SRR11306495	Schulz Bank (MRGID:5039)	897	GOSars2017	LINESTRING(7.4447 73.8142, 7.4452 73.8147)
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#20	Craniella_infrequens_adult	in_situ	WB_00913	insdc:SRR11306494	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#21_embryos	Craniella_infrequens_embryo	in_situ	WB_00914_embryos	insdc:SRR11306493	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#21	Craniella_infrequens_adult	in_situ	WB_00914	insdc:SRR11306492	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#22	Craniella_infrequens_adult	in_situ	WB_00915	insdc:SRR11306489	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#23	Craniella_infrequens_adult	in_situ	WB_00916	insdc:SRR11306488	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#105	Craniella_infrequens_juvenile	in_situ	WB_00917	insdc:SRR11306487	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#106	Craniella_infrequens_juvenile	in_situ	WB_00918	insdc:SRR11306486	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#107	Craniella_infrequens_juvenile	in_situ	WB_00919	insdc:SRR11306485	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#108	Craniella_infrequens_juvenile	in_situ	WB_00920	insdc:SRR11306484	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#109	Craniella_infrequens_juvenile	in_situ	WB_00921	insdc:SRR11306483	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#16	Craniella_infrequens_juvenile	in_situ	WB_00922	insdc:SRR11306482	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#17	Craniella_infrequens_juvenile	in_situ	WB_00923	insdc:SRR11306481	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#18	Craniella_infrequens_juvenile	in_situ	WB_00924	insdc:SRR11306480	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#19	Craniella_infrequens_juvenile	in_situ	WB_00925	insdc:SRR11306478	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#24	Craniella_zetlandica_adult	in_situ	WB_00927	insdc:SRR11306477	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#25	Craniella_zetlandica_adult	in_situ	WB_00928	insdc:SRR11306476	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#26	Craniella_zetlandica_adult	in_situ	WB_00929	insdc:SRR11306475	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-17-AGT-01	2018-08-02T00:00	73.8369	7.5681	Trawl, Agassiz (AGT)	GS2018108-17-AGT-1#27	Craniella_zetlandica_adult	in_situ	WB_00930	insdc:SRR11306474	Schulz Bank (MRGID:5039)	587	GOSars2018
GS2018108-64-ROV-48	2018-08-11T16:14	70.2623	22.4814	Remotely operated vehicle (ROV)	GS2018108-64-ROV-48#3	Craniella_zetlandica_adult	in_situ	WB_01006	insdc:SRR11306473	Finnmark (MRGID:8962) : Stjernsund	251	GOSars2018	LINESTRING(22.4966 70.2606, 22.4758 70.2622)
GS2018108-64-ROV-48	2018-08-11T16:14	70.2623	22.4814	Remotely operated vehicle (ROV)	GS2018108-64-ROV-48#3	Craniella_zetlandica_embryo	in_situ	WB_01006_embryos	insdc:SRR11306472	Finnmark (MRGID:8962) : Stjernsund	251	GOSars2018	LINESTRING(22.4966 70.2606, 22.4758 70.2622)
GS2018108-70-ROV-50	2018-08-12T20:39	70.2632	22.4744	Remotely operated vehicle (ROV)	GS2018108-70-ROV-50#6	Craniella_zetlandica_adult	in_situ	WB_01014	insdc:SRR11306471	Finnmark (MRGID:8962) : Stjernsund	218	GOSars2018	LINESTRING(22.4763 70.2623, 22.4680 70.2664)
GS2018108-70-ROV-50	2018-08-12T20:39	70.2632	22.4744	Remotely operated vehicle (ROV)	GS2018108-70-ROV-50#7	Craniella_zetlandica_adult	in_situ	WB_01015	insdc:SRR11306470	Finnmark (MRGID:8962) : Stjernsund	218	GOSars2018	LINESTRING(22.4763 70.2623, 22.4680 70.2664)
GS2018108-78-ROV-52	2018-08-13T10:51	70.2702	22.4759	Remotely operated vehicle (ROV)	GS2018108-78-ROV-52#3	Craniella_zetlandica_adult	in_situ	WB_01025	insdc:SRR11306469	Finnmark (MRGID:8962) : Stjernsund	241	GOSars2018	LINESTRING(22.4639 70.2656, 22.4891 70.2765)
AQUARIUM_C.zetlandica	2018-09-19T00:00			Experiment (EXP)	AQUARIUM#1	Craniella_zetlandica_juvenile	aquarium	WB_01075	insdc:SRR11306467
AQUARIUM_C.zetlandica	2018-09-19T00:00			Experiment (EXP)	AQUARIUM#2	Craniella_zetlandica_juvenile	aquarium	WB_01076	insdc:SRR11306466
AQUARIUM_C.zetlandica	2018-09-19T00:00			Experiment (EXP)	AQUARIUM#3	Craniella_zetlandica_juvenile	aquarium	WB_01077	insdc:SRR11306465
AQUARIUM_C.zetlandica	2018-09-19T00:00			Experiment (EXP)	AQUARIUM#4	Craniella_zetlandica_juvenile	aquarium	WB_01078	insdc:SRR11306464
AQUARIUM_C.zetlandica	2018-09-19T00:00			Experiment (EXP)	AQUARIUM#5	Craniella_zetlandica_juvenile	aquarium	WB_01087	insdc:SRR11306463
AQUARIUM_C.zetlandica	2018-09-19T00:00			Experiment (EXP)	AQUARIUM#6	Craniella_zetlandica_juvenile	aquarium	WB_01088	insdc:SRR11306462
AQUARIUM_C.zetlandica	2018-09-19T00:00			Experiment (EXP)	AQUARIUM#7	Craniella_zetlandica_juvenile	aquarium	WB_01089	insdc:SRR11306461
AQUARIUM_C.zetlandica	2018-09-19T00:00			Experiment (EXP)	AQUARIUM#8	Craniella_zetlandica_juvenile	aquarium	WB_01090	insdc:SRR11306460
AQUARIUM_C.zetlandica	2018-09-19T00:00			Experiment (EXP)	AQUARIUM#9	Craniella_zetlandica_juvenile	aquarium	WB_01091	insdc:SRR11306459
GS2017110-15-CTD-05	2017-07-27T00:47	73.8003	7.3986	CTD/Rosette (CTD-RO)	GS2017110-15-CTD5	Seawater	in_situ	SW-00163	insdc:SRR11306458	Schulz Bank (MRGID:5039)	1070	GOSars2017	POINT(7.398617 73.80025)
GS2017110-15-CTD-05	2017-07-27T00:47	73.8003	7.3986	CTD/Rosette (CTD-RO)	GS2017110-15-CTD5	Seawater	in_situ	SW-00164	insdc:SRR11306510	Schulz Bank (MRGID:5039)	1070	GOSars2017	POINT(7.398617 73.80025)
GS2017110-15-CTD-05	2017-07-27T00:47	73.8003	7.3986	CTD/Rosette (CTD-RO)	GS2017110-15-CTD5	Seawater	in_situ	SW-00165	insdc:SRR11306509	Schulz Bank (MRGID:5039)	1070	GOSars2017	POINT(7.398617 73.80025)
GS2018108-22-CTD-07	2018-08-03T11:23	73.8299	7.5624	CTD/Rosette (CTD-RO)	GS2018108-22-CTD-07	Seawater	in_situ	SW-00307	insdc:SRR11306508	Schulz Bank (MRGID:5039)	577	GOSars2018	POINT(7.5624 73.8299)
GS2018108-22-CTD-07	2018-08-03T11:23	73.8299	7.5624	CTD/Rosette (CTD-RO)	GS2018108-22-CTD-07	Seawater	in_situ	SW-00308	insdc:SRR11306507	Schulz Bank (MRGID:5039)	577	GOSars2018	POINT(7.5624 73.8299)
GS2018108-22-CTD-07	2018-08-03T11:23	73.8299	7.5624	CTD/Rosette (CTD-RO)	GS2018108-22-CTD-07	Seawater	in_situ	SW-00309	insdc:SRR11306506	Schulz Bank (MRGID:5039)	577	GOSars2018	POINT(7.5624 73.8299)
GS2018108-66-CTD-16	2018-08-12T12:09	70.2622	22.4771	CTD/Rosette (CTD-RO)	GS2018108-66-CTD-16	Seawater	in_situ	SW-00349	insdc:SRR11306505	Finnmark (MRGID:8962) : Stjernsund	217	GOSars2018	POINT(22.4771 70.2622)
GS2018108-66-CTD-16	2018-08-12T12:09	70.2622	22.4771	CTD/Rosette (CTD-RO)	GS2018108-66-CTD-16	Seawater	in_situ	SW-00350	insdc:SRR11306504	Finnmark (MRGID:8962) : Stjernsund	217	GOSars2018	POINT(22.4771 70.2622)
GS2018108-66-CTD-16	2018-08-12T12:09	70.2622	22.4771	CTD/Rosette (CTD-RO)	GS2018108-66-CTD-16	Seawater	in_situ	SW-00351	insdc:SRR11306503	Finnmark (MRGID:8962) : Stjernsund	217	GOSars2018	POINT(22.4771 70.2622)
GS2018108-77-CTD-24	2018-08-13T07:20	70.2631	22.4755	CTD/Rosette (CTD-RO)	GS2018108-77-CTD-24	Seawater	in_situ	SW-00352	insdc:SRR11306502	Finnmark (MRGID:8962) : Stjernsund	218	GOSars2018	POINT(22.4755 70.2631)
GS2018108-77-CTD-24	2018-08-13T07:20	70.2631	22.4755	CTD/Rosette (CTD-RO)	GS2018108-77-CTD-24	Seawater	in_situ	SW-00353	insdc:SRR11306501	Finnmark (MRGID:8962) : Stjernsund	218	GOSars2018	POINT(22.4755 70.2631)
GS2018108-77-CTD-24	2018-08-13T07:20	70.2631	22.4755	CTD/Rosette (CTD-RO)	GS2018108-77-CTD-24	Seawater	in_situ	SW-00354	insdc:SRR11306499	Finnmark (MRGID:8962) : Stjernsund	218	GOSars2018	POINT(22.4755 70.2631)
GS2018108-78-ROV-52	2018-08-13T10:52	70.2656	22.4639	Bottle, Niskin (ROV)	GS2018108-78-ROV-52	Seawater	in_situ	SW-00355	insdc:SRR11306498	Finnmark (MRGID:8962) : Stjernsund	301	GOSars2018	LINESTRING(22.4639 70.2656, 22.4891 70.2765)
GS2018108-78-ROV-52	2018-08-13T10:52	70.2656	22.4639	Bottle, Niskin (ROV)	GS2018108-78-ROV-52	Seawater	in_situ	SW-00356	insdc:SRR11306497	Finnmark (MRGID:8962) : Stjernsund	301	GOSars2018	LINESTRING(22.4639 70.2656, 22.4891 70.2765)
GS2018108-78-ROV-52	2018-08-13T10:52	70.2656	22.4639	Bottle, Niskin (ROV)	GS2018108-78-ROV-52	Seawater	in_situ	SW-00357	insdc:SRR11306496	Finnmark (MRGID:8962) : Stjernsund	301	GOSars2018	LINESTRING(22.4639 70.2656, 22.4891 70.2765)