Weber, Judith Sophie; Shaida, Stephen Saikiu; Ngomtcho, Sen Claudine Henriette; Musa, Usman Baba; Adamu, Ahmadu; Gbem, Thaddeus Terlumun; Berger, Petra; Mamman, Mohammed; Nok, Jonathan Andrew; Kelm, Sørge (2020): Sialidase assay and analysis of free sialic acid in tsetse (Glossina sp.) collected in protected and tsetse invested areas in Nigeria [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.913420, In: Weber, JS et al. (2020): Molecular and morphological characterization of Glossina sp. and identification of associated Trypanosoma sp. in Nigeria including analysis for sialidase activity [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.915145
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Published: 2020-03-12 • DOI registered: 2020-07-07
Abstract:
This data set includes results of a sialidase assay applied in tsetse gut tissue from a tsetse (Glossina sp.) collection carried out in Nigeria between 2014 and 2016. Traps were employed for one to three days in four different National Parks, one Game Reserve and two tsetse invested areas. Each tsetse was identified morphologically and dissected in the field, all tissues preserved and used for further analysis. The whole gut of each tsetse was homogenized in the field and partially preserved for DNA analysis and used to investigate for sialidase activity with a fluorescent substrate and a natural substrate. Analysis for sialidase activity was done by fluorescent measurement and reverse phase HPLC of processed gut samples.
Related to:
Shaida, Stephen Saikiu; Weber, Judith Sophie; Gbem, Thaddeus Terlumun; Ngomtcho, Sen Claudine Henriette; Musa, Usman Baba; Achukwi, Mbunkah Daniel; Mamman, Mohammed; Ndams, Iliya Shehu; Nok, Jonathan Andrew; Kelm, Sørge (2018): Diversity and phylogenetic relationships of Glossina populations in Nigeria and the Cameroonian border region. BMC Microbiology, 18(S1), https://doi.org/10.1186/s12866-018-1293-6
Further details:
Hara, Shuuji; Yamaguchi, Masatoshi; Takemori, Yasuyo; Yoshitake, Takashi; Nakamura, Masaru (1988): 1,2-diamino-4,5-methylenedioxybenzene as a highly sensitive fluorogenic reagent for α-dicarbonyl compounds. Analytica Chimica Acta, 215, 267-276, https://doi.org/10.1016/S0003-2670(00)85285-X
Wang, Libo; Wang, John D; Zhou, Xiang; Wu, Lijun; Sun, Xue-Long (2014): Systematic investigation of quinoxaline derivatization of sialic acids and their quantitation applicability using high performance liquid chromatography. RSC Advances, 4(86), 45797-45803, https://doi.org/10.1039/c4ra08930h
Project(s):
Coverage:
Median Latitude: 8.815657 * Median Longitude: 6.544343 * South-bound Latitude: 5.361000 * West-bound Longitude: 3.773000 * North-bound Latitude: 9.912000 * East-bound Longitude: 11.738000
Date/Time Start: 2014-02-23T12:07:00 * Date/Time End: 2016-03-21T00:00:00
Event(s):
234-CRNP-T003b * Latitude: 5.364000 * Longitude: 8.457000 * Date/Time: 2014-03-16T16:55:00 * Location: Cross River National Park, Nigeria, Africa * Method/Device: Insecttrap, biconical (BICON) * Comment: Vegetation: Rainforest/ Close by small stream
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Kelm, Sørge | |||
| 2 | Sample ID | Sample ID | Kelm, Sørge | |||
| 3 | Collector | Collector | Kelm, Sørge | |||
| 4 | Treatment | Treat | Kelm, Sørge | |||
| 5 | Analytical method | Analyt method | Kelm, Sørge | Assay | ||
| 6 | Analytical method | Analyt method | Kelm, Sørge | Compound detected | ||
| 7 | Gear | Gear | Kelm, Sørge | Plate reader | ||
| 8 | Wavelength | Lambda | nm | Kelm, Sørge | Excitation wavelength | |
| 9 | Wavelength | Lambda | nm | Kelm, Sørge | Emission wavelength | |
| 10 | Relative fluorescence intensity, ratio | Q | Kelm, Sørge | Relative fluorescence increase upon substrate addition over the control | ||
| 11 | Treatment | Treat | Kelm, Sørge | Derviatization agent | ||
| 12 | Analytical method | Analyt method | Kelm, Sørge | |||
| 13 | Gear | Gear | Kelm, Sørge | RP-HPLC System | ||
| 14 | Reference/source | Reference | Kelm, Sørge | |||
| 15 | Subsample ID | Subsample ID | Kelm, Sørge | |||
| 16 | Sialic acid, free | SIA free | µmol/l | Kelm, Sørge | ||
| 17 | Subsample ID | Subsample ID | Kelm, Sørge | |||
| 18 | Sialic acid, free | SIA free | µmol/l | Kelm, Sørge | ||
| 19 | Subsample ID | Subsample ID | Kelm, Sørge | |||
| 20 | Sialic acid, free | SIA free | µmol/l | Kelm, Sørge |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
5968 data points
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