Not logged in
PANGAEA.
Data Publisher for Earth & Environmental Science

Weber, Judith Sophie; Shaida, Stephen Saikiu; Ngomtcho, Sen Claudine Henriette; Musa, Usman Baba; Adamu, Ahmadu; Gbem, Thaddeus Terlumun; Berger, Petra; Mamman, Mohammed; Nok, Jonathan Andrew; Kelm, Sørge (2020): Molecular and morphological characterization of Glossina sp. and identification of associated Trypanosoma sp. in Nigeria including analysis for sialidase activity [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.915145

Always quote citation above when using data! You can download the citation in several formats below.

Published: 2020-04-23 • DOI registered: 2020-05-22

RIS CitationBibTeX Citation ShareShow MapGoogle Earth

Abstract:
These data sets include morphological and molecular data generated from a tsetse (Glossina sp.) collection carried out in Nigeria between 2014 and 2016. Traps were employed for one to three days in four different National Parks (Kainji Lake National Park, Old Oyo National Park, Cross River National Park, Gashaka Gumti National Park), one Game Reserve (Yankari Game Reserve) and two tsetse invested areas (Ijah Gwari, Tuongo). Each tsetse was identified morphologically and dissected in the field, all tissues preserved and used for further analysis. Selected tsetse wings were mounted and photographed to be used for morphometric analysis. The whole gut of each tsetse was homogenized in the field and partially preserved for DNA analysis and used to investigate for sialidase activity with a fluorescent substrate and a natural substrate. Analysis for sialidase activity was done by fluorescent measurement and reverse phase HPLC of processed gut samples. DNA was purified from preserved homogenized gut and used for phylogenetic analysis of tsetse by amplification of Cytochrome Oxidase C SU1 and Internal Transcribed Spacer-1 (ITS1). Presence of trypanosomes was investigated in gut and crudely extracted DNA from proboscis by amplification of ITS1. Identification of the respective trypanosome species was done based on lengths polymorphism of the ITS1 region as well as sequencing of selected ITS1 amplicons and additional glycosomal Glyceraldehyde-3-phosohate dehydrogenase.
Related to:
Shaida, Stephen Saikiu; Weber, Judith Sophie; Gbem, Thaddeus Terlumun; Ngomtcho, Sen Claudine Henriette; Musa, Usman Baba; Achukwi, Mbunkah Daniel; Mamman, Mohammed; Ndams, Iliya Shehu; Nok, Jonathan Andrew; Kelm, Sørge (2018): Diversity and phylogenetic relationships of Glossina populations in Nigeria and the Cameroonian border region. BMC Microbiology, 18(S1), https://doi.org/10.1186/s12866-018-1293-6
Weber, Judith Sophie; Ngomtcho, Sen Claudine Henriette; Shaida, Stephen Saikiu; Chechet, Gloria Dada; Gbem, Thaddeus Terlumun; Nok, Jonathan Andrew; Mamman, Mohammed; Achukwi, Mbunkah Daniel; Kelm, Sørge (2019): Genetic diversity of trypanosome species in tsetse flies (Glossina spp.) in Nigeria. Parasites & Vectors, 12(1), https://doi.org/10.1186/s13071-019-3718-y
Coverage:
Median Latitude: 8.065124 * Median Longitude: 7.117572 * South-bound Latitude: 5.328000 * West-bound Longitude: 3.773000 * North-bound Latitude: 9.912000 * East-bound Longitude: 12.100000
Date/Time Start: 2014-02-22T00:00:00 * Date/Time End: 2016-03-22T13:35:00
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
6 datasets

Download Data

Download ZIP file containing all datasets as tab-delimited text — use the following character encoding:

Datasets listed in this publication series

  1. Weber, JS; Shaida, SS; Ngomtcho, SCH et al. (2020): Sialidase assay and analysis of free sialic acid in tsetse (Glossina sp.) collected in protected and tsetse invested areas in Nigeria. https://doi.org/10.1594/PANGAEA.913420
  2. Weber, JS; Shaida, SS; Ngomtcho, SCH et al. (2020): Tryapanosoma prevalence in tsetse (Glossina sp.) gut collected in protected and tsetse invested areas in Nigeria. https://doi.org/10.1594/PANGAEA.913421
  3. Weber, JS; Shaida, SS; Ngomtcho, SCH et al. (2020): Tryapanosoma prevalence in tsetse proboscis (Glossina sp.) collected in protected and tsetse invested areas in Nigeria. https://doi.org/10.1594/PANGAEA.913422
  4. Weber, JS; Shaida, SS; Ngomtcho, SCH et al. (2020): Collected tsetse tissue and tsetse species (Glossina sp.) collected in protected and tsetse invested areas in Nigeria. https://doi.org/10.1594/PANGAEA.913417
  5. Weber, JS; Shaida, SS; Ngomtcho, SCH et al. (2020): Environmental data and tsetse numbers per trap in protected and tsetse invested areas in Nigeria. https://doi.org/10.1594/PANGAEA.913416
  6. Weber, JS; Shaida, SS; Ngomtcho, SCH et al. (2020): Tsetse wing pictures (Glossina sp.) from tsetse collected in protected and tsetse invested areas in Nigeria. https://doi.org/10.1594/PANGAEA.913437