Citation:

rsos.191041

Name: Seawater carbonate chemistry and acid–base physiology and behaviour of the California sea hare (Aplysia californica)
Published: 2019
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Acid-base regulation; Animalia; Aplysia californica; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Laboratory experiment; Mollusca; North Atlantic; Single species; Temperate

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Abstract:

Behavioural impairment following exposure to ocean acidification-relevant CO2 levels has been noted in a broad array of taxa. The underlying cause of these disruptions is thought to stem from alterations of ion gradients ([HCO3]−/Cl−) across neuronal cell membranes that occur as a consequence of maintaining pH homeostasis via the accumulation of [HCO3]−. While behavioural impacts are widely documented, few studies have measured acid–base parameters in species showing behavioural disruptions. In addition, current studies examining mechanisms lack resolution in targeting specific neural pathways corresponding to a given behaviour. With these considerations in mind, acid–base parameters and behaviour were measured in a model organism used for decades as a research model to study learning, the California sea hare (Aplysia californica). Aplysia exposed to elevated CO2 increased haemolymph [HCO3]−, achieving full and partial pH compensation at 1200 and 3000 µatm CO2, respectively. Increased CO2 did not affect self-righting behaviour. In contrast, both levels of elevated CO2 reduced the time of the tail-withdrawal reflex, suggesting a reduction in antipredator response. Overall, these results confirm that Aplysia are promising models to examine mechanisms underlying CO2-induced behavioural disruptions since they regulate [HCO3]− and have behaviours linked to neural networks amenable to electrophysiological testing.

Keyword(s):

Acid-base regulation; Animalia; Aplysia californica; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Laboratory experiment; Mollusca; North Atlantic; Single species; Temperate

Original version:

Zlatkin, Rebecca L; Heuer, Rachael M (2019): Data from: Ocean acidification affects acid-base physiology and behaviour in a model invertebrate, the California sea hare (Aplysia californica). Dryad, https://doi.org/10.5061/dryad.7pd654v

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-02-17.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Heuer, Rachael M		study
2	Species	Species		Heuer, Rachael M
3	Registration number of species	Reg spec no		Heuer, Rachael M
4	Uniform resource locator/link to reference	URL ref		Heuer, Rachael M		WoRMS Aphia ID
5	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Heuer, Rachael M
6	Experiment	Exp		Heuer, Rachael M
7	Identification	ID		Heuer, Rachael M
8	Time in seconds	Time	s	Heuer, Rachael M		Trial 1
9	Time in seconds	Time	s	Heuer, Rachael M		Trial 2
10	Time in seconds	Time	s	Heuer, Rachael M		Trial 3
11	Time in seconds	Time	s	Heuer, Rachael M		Time to relax tail average of three trials (s)
12	Comment	Comment		Heuer, Rachael M		Reason for no data
13	Order	Order		Heuer, Rachael M
14	Time in days	Time	days	Heuer, Rachael M		Exposure time
15	Percentage	Perc	%	Heuer, Rachael M		Trial 1, contracted
16	Percentage	Perc	%	Heuer, Rachael M		Trial 2, contracted
17	Percentage	Perc	%	Heuer, Rachael M		Trial 3, contracted
18	Percentage	Perc	%	Heuer, Rachael M		Contracted
19	Comment	Comment		Heuer, Rachael M		Reason for no data
20	Mass	Mass	g	Heuer, Rachael M		Body
21	Haemolymph, pH	pH (ha)		Heuer, Rachael M		NBS scale
22	Haemolymph, bicarbonate ion	[HCO3]- (ha)	mmol/l	Heuer, Rachael M
23	Haemolymph, partial pressure of carbon dioxide	pCO2 (ha)	mm Hg	Heuer, Rachael M
24	Righting time	Righting time	s	Heuer, Rachael M		Trial 1
25	Righting time	Righting time	s	Heuer, Rachael M		Trial 2
26	Righting time	Righting time	s	Heuer, Rachael M		Trial 3
27	Righting time	Righting time	s	Heuer, Rachael M
28	Comment	Comment		Heuer, Rachael M		Note for GFR
29	Identification	ID		Heuer, Rachael M		Tank
30	Salinity	Sal		Heuer, Rachael M
31	Salinity, standard error	Sal std e	±	Heuer, Rachael M
32	Temperature, water	Temp	°C	Heuer, Rachael M
33	Temperature, water, standard error	T std e	±	Heuer, Rachael M
34	pH	pH		Heuer, Rachael M		NBS scale
35	pH, standard error	pH std e	±	Heuer, Rachael M		NBS scale
36	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Heuer, Rachael M	Calculated using CO2SYS
37	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Heuer, Rachael M	Calculated using CO2SYS
38	Alkalinity, total	AT	µmol/kg	Heuer, Rachael M	Calculated using CO2SYS
39	Alkalinity, total, standard error	AT std e	±	Heuer, Rachael M	Calculated using CO2SYS
40	Carbon, inorganic, dissolved	DIC	µmol/kg	Heuer, Rachael M	Calculated using CO2SYS
41	Carbon, inorganic, dissolved, standard error	DIC std e	±	Heuer, Rachael M	Calculated using CO2SYS
42	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
44	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

4858 data points

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