Citation:

rsos.191041

Name: Seawater carbonate chemistry and acid–base physiology and behaviour of the California sea hare (Aplysia californica)
Published: 2019
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Acid-base regulation; Animalia; Aplysia californica; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Laboratory experiment; Mollusca; North Atlantic; Single species; Temperate

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Abstract:

Behavioural impairment following exposure to ocean acidification-relevant CO2 levels has been noted in a broad array of taxa. The underlying cause of these disruptions is thought to stem from alterations of ion gradients ([HCO3]−/Cl−) across neuronal cell membranes that occur as a consequence of maintaining pH homeostasis via the accumulation of [HCO3]−. While behavioural impacts are widely documented, few studies have measured acid–base parameters in species showing behavioural disruptions. In addition, current studies examining mechanisms lack resolution in targeting specific neural pathways corresponding to a given behaviour. With these considerations in mind, acid–base parameters and behaviour were measured in a model organism used for decades as a research model to study learning, the California sea hare (Aplysia californica). Aplysia exposed to elevated CO2 increased haemolymph [HCO3]−, achieving full and partial pH compensation at 1200 and 3000 µatm CO2, respectively. Increased CO2 did not affect self-righting behaviour. In contrast, both levels of elevated CO2 reduced the time of the tail-withdrawal reflex, suggesting a reduction in antipredator response. Overall, these results confirm that Aplysia are promising models to examine mechanisms underlying CO2-induced behavioural disruptions since they regulate [HCO3]− and have behaviours linked to neural networks amenable to electrophysiological testing.

Keyword(s):

Acid-base regulation; Animalia; Aplysia californica; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Laboratory experiment; Mollusca; North Atlantic; Single species; Temperate

Original version:

Zlatkin, Rebecca L; Heuer, Rachael M (2019): Data from: Ocean acidification affects acid-base physiology and behaviour in a model invertebrate, the California sea hare (Aplysia californica). Dryad, https://doi.org/10.5061/dryad.7pd654v

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-02-17.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Heuer, Rachael M		study
2	Species	Species		Heuer, Rachael M
3	Registration number of species	Reg spec no		Heuer, Rachael M
4	Uniform resource locator/link to reference	URL ref		Heuer, Rachael M		WoRMS Aphia ID
5	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Heuer, Rachael M
6	Experiment	Exp		Heuer, Rachael M
7	Identification	ID		Heuer, Rachael M
8	Time in seconds	Time	s	Heuer, Rachael M		Trial 1
9	Time in seconds	Time	s	Heuer, Rachael M		Trial 2
10	Time in seconds	Time	s	Heuer, Rachael M		Trial 3
11	Time in seconds	Time	s	Heuer, Rachael M		Time to relax tail average of three trials (s)
12	Comment	Comment		Heuer, Rachael M		Reason for no data
13	Order	Order		Heuer, Rachael M
14	Time in days	Time	days	Heuer, Rachael M		Exposure time
15	Percentage	Perc	%	Heuer, Rachael M		Trial 1, contracted
16	Percentage	Perc	%	Heuer, Rachael M		Trial 2, contracted
17	Percentage	Perc	%	Heuer, Rachael M		Trial 3, contracted
18	Percentage	Perc	%	Heuer, Rachael M		Contracted
19	Comment	Comment		Heuer, Rachael M		Reason for no data
20	Mass	Mass	g	Heuer, Rachael M		Body
21	Haemolymph, pH	pH (ha)		Heuer, Rachael M		NBS scale
22	Haemolymph, bicarbonate ion	[HCO3]- (ha)	mmol/l	Heuer, Rachael M
23	Haemolymph, partial pressure of carbon dioxide	pCO2 (ha)	mm Hg	Heuer, Rachael M
24	Righting time	Righting time	s	Heuer, Rachael M		Trial 1
25	Righting time	Righting time	s	Heuer, Rachael M		Trial 2
26	Righting time	Righting time	s	Heuer, Rachael M		Trial 3
27	Righting time	Righting time	s	Heuer, Rachael M
28	Comment	Comment		Heuer, Rachael M		Note for GFR
29	Identification	ID		Heuer, Rachael M		Tank
30	Salinity	Sal		Heuer, Rachael M
31	Salinity, standard error	Sal std e	±	Heuer, Rachael M
32	Temperature, water	Temp	°C	Heuer, Rachael M
33	Temperature, water, standard error	T std e	±	Heuer, Rachael M
34	pH	pH		Heuer, Rachael M		NBS scale
35	pH, standard error	pH std e	±	Heuer, Rachael M		NBS scale
36	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Heuer, Rachael M	Calculated using CO2SYS
37	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Heuer, Rachael M	Calculated using CO2SYS
38	Alkalinity, total	AT	µmol/kg	Heuer, Rachael M	Calculated using CO2SYS
39	Alkalinity, total, standard error	AT std e	±	Heuer, Rachael M	Calculated using CO2SYS
40	Carbon, inorganic, dissolved	DIC	µmol/kg	Heuer, Rachael M	Calculated using CO2SYS
41	Carbon, inorganic, dissolved, standard error	DIC std e	±	Heuer, Rachael M	Calculated using CO2SYS
42	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
44	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

4858 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 pCO2water_SST_wet [µatm]	6 Exp	7 ID	8 Time [s] (Trial 1)	9 Time [s] (Trial 2)	10 Time [s] (Trial 3)	11 Time [s] (Time to relax tail average of...)	12 Comment (Reason for no data)	13 Order	14 Time [days] (Exposure time)	15 Perc [%] (Trial 1, contracted)	16 Perc [%] (Trial 2, contracted)	17 Perc [%] (Trial 3, contracted)	18 Perc [%] (Contracted)	19 Comment (Reason for no data)	20 Mass [g] (Body)	21 pH (ha) (NBS scale)	22 [HCO3]- (ha) [mmol/l]	23 pCO2 (ha) [mm Hg]	24 Righting time [s] (Trial 1)	25 Righting time [s] (Trial 2)	26 Righting time [s] (Trial 3)	27 Righting time [s]	28 Comment (Note for GFR)	29 ID (Tank)	30 Sal	31 Sal std e [±]	32 Temp [°C]	33 T std e [±]	34 pH (NBS scale)	35 pH std e [±] (NBS scale)	36 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	37 pCO2water_SST_wet std e [±] (Calculated using CO2SYS)	38 AT [µmol/kg] (Calculated using CO2SYS)	39 AT std e [±] (Calculated using CO2SYS)	40 DIC [µmol/kg] (Calculated using CO2SYS)	41 DIC std e [±] (Calculated using CO2SYS)	42 CSC flag (Calculated using seacarb afte...)	43 pH (total scale, Calculated using...)	44 CO2 [µmol/kg] (Calculated using seacarb afte...)	45 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	46 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	47 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	48 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	49 AT [µmol/kg] (Calculated using seacarb afte...)	50 Omega Arg (Calculated using seacarb afte...)	51 Omega Cal (Calculated using seacarb afte...)
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 1	15.44	24.00	7.00	15.48		2	11	53.3	60.00	60.0	57.77		97.0										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 2	12.00	8.00	6.69	8.90		2	11	56.3	68.80	56.3	60.47		111.0										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 3	13.00		28.70	20.85	Full contraction not observed	2	11	53.3		53.3	53.30	Full contraction not observed	96.5										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 4		22.00	47.00	34.50	Relaxation response not clear	1	4	60.0	60.00	40.0	53.33		105.0										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 5	14.79	17.74	16.00	16.18		1	4	28.6	42.90	53.8	41.77		73.0										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 6	8.85	9.00	7.71	8.52		1	4	58.3	46.20	58.3	54.27		70.0										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 7	26.43	43.35	47.30	39.03		2	11	52.9	50.00	56.3	53.07		121.5										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 8	9.23	7.37	7.22	7.94		2	11	66.7	58.10	60.0	61.60		112.5										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 9	7.11	5.48	27.63	13.41		1	4	56.3	60.00	62.5	59.60		97.0										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 10	32.36		28.88	30.62	Relaxation response not clear	1	4	50.0	56.25	62.5	56.25		106.0										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 11	31.11	16.16	28.18	25.15		1	4	37.5	50.00	53.3	46.93		111.0										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 12	18.02	20.39	13.83	17.41		1	4	53.8	53.80	42.9	50.17		101.5										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 13	25.46	21.35	17.71	21.51		1	4	57.1	57.10	57.1	57.10		120.5										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 14	40.59	38.25	38.03	38.96		1	4	40.0	40.00	53.3	44.43		81.0										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 15	13.33	20.26	15.83	16.47		1	4	53.3	46.70	46.7	48.90		75.0										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 16	28.85	12.75	32.38	24.66		1	4	61.5	46.70	46.7	51.63		83.0										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 17	47.80	31.23	21.28	33.44		1	4	42.9	50.00	42.9	45.27		87.5										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 18	43.08	49.16	50.13	47.46		1	4	42.9	42.90	46.2	44.00		81.0										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Tail withdrawal	Animal 19	44.72	11.93	27.10	27.92		1	4	53.8	53.80	61.5	56.37		70.0										32.9	0.4	14.9	0.05	8.17	0.008	411.3	18.0	2284.5	47.9	2081.8	47.00	27	8.05	15.01	394.70	396.13	1909.52	152.18	2284.5	2.35	3.68
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Tail withdrawal	Animal 1	11.00	12.00	12.00	11.67		1	4	57.1	64.30	57.1	59.50		91.5										33.1	0.4	14.9	0.08	7.77	0.006	1146.4	23.7	2394.8	41.2	2334.7	39.80	27	7.65	43.76	1152.35	1156.54	2221.59	70.84	2394.8	1.09	1.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Tail withdrawal	Animal 2	10.00	16.41	15.62	14.01		1	4	42.9	37.00	41.7	40.53		51.0										33.1	0.4	14.9	0.08	7.77	0.006	1146.4	23.7	2394.8	41.2	2334.7	39.80	27	7.65	43.76	1152.35	1156.54	2221.59	70.84	2394.8	1.09	1.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Tail withdrawal	Animal 3	15.66	16.26		15.96	Full contraction not observed	1	4	50.0	69.20		59.60		65.5										33.1	0.4	14.9	0.08	7.77	0.006	1146.4	23.7	2394.8	41.2	2334.7	39.80	27	7.65	43.76	1152.35	1156.54	2221.59	70.84	2394.8	1.09	1.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Tail withdrawal	Animal 4	11.98	7.26	10.59	9.94		2	11	50.0	62.10	57.1	56.40		107.0										33.1	0.4	14.9	0.08	7.77	0.006	1146.4	23.7	2394.8	41.2	2334.7	39.80	27	7.65	43.76	1152.35	1156.54	2221.59	70.84	2394.8	1.09	1.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Tail withdrawal	Animal 5	8.13	6.78	10.59	8.50		2	11	57.1	62.10	60.0	59.73		110.0										33.1	0.4	14.9	0.08	7.77	0.006	1146.4	23.7	2394.8	41.2	2334.7	39.80	27	7.65	43.76	1152.35	1156.54	2221.59	70.84	2394.8	1.09	1.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Tail withdrawal	Animal 6	15.68	12.57	13.98	14.08		2	11	53.3	75.00	53.3	60.53		121.0										33.1	0.4	14.9	0.08	7.77	0.006	1146.4	23.7	2394.8	41.2	2334.7	39.80	27	7.65	43.76	1152.35	1156.54	2221.59	70.84	2394.8	1.09	1.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Tail withdrawal	Animal 7	42.82	10.92	18.21	23.98		2	11	56.3	46.70	60.0	54.33		126.0										33.1	0.4	14.9	0.08	7.77	0.006	1146.4	23.7	2394.8	41.2	2334.7	39.80	27	7.65	43.76	1152.35	1156.54	2221.59	70.84	2394.8	1.09	1.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Tail withdrawal	Animal 8	29.13	9.18	27.53	21.95		1	4	60.0	53.30	46.7	53.33		95.5										33.1	0.4	14.9	0.08	7.77	0.006	1146.4	23.7	2394.8	41.2	2334.7	39.80	27	7.65	43.76	1152.35	1156.54	2221.59	70.84	2394.8	1.09	1.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Tail withdrawal	Animal 9	12.07	13.83	26.09	17.33		1	4	50.0	56.30	40.0	48.77		102.0										33.1	0.4	14.9	0.08	7.77	0.006	1146.4	23.7	2394.8	41.2	2334.7	39.80	27	7.65	43.76	1152.35	1156.54	2221.59	70.84	2394.8	1.09	1.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Tail withdrawal	Animal 10	9.71	11.25	9.92	10.29		1	4	46.2	46.20	53.8	48.73		86.5										33.1	0.4	14.9	0.08	7.77	0.006	1146.4	23.7	2394.8	41.2	2334.7	39.80	27	7.65	43.76	1152.35	1156.54	2221.59	70.84	2394.8	1.09	1.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Tail withdrawal	Animal 11	6.06	6.11	7.38	6.52		1	4	60.0	64.30	57.1	60.47		101.0										33.1	0.4	14.9	0.08	7.77	0.006	1146.4	23.7	2394.8	41.2	2334.7	39.80	27	7.65	43.76	1152.35	1156.54	2221.59	70.84	2394.8	1.09	1.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Tail withdrawal	Animal 12	12.36	11.25	23.65	15.75		1	4	57.1	61.50	53.8	57.47		71.0										33.1	0.4	14.9	0.08	7.77	0.006	1146.4	23.7	2394.8	41.2	2334.7	39.80	27	7.65	43.76	1152.35	1156.54	2221.59	70.84	2394.8	1.09	1.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Tail withdrawal	Animal 13	9.53	13.82	8.88	10.74		1	4	46.2	53.80	61.5	53.83		77.5										33.1	0.4	14.9	0.08	7.77	0.006	1146.4	23.7	2394.8	41.2	2334.7	39.80	27	7.65	43.76	1152.35	1156.54	2221.59	70.84	2394.8	1.09	1.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Tail withdrawal	Animal 14	12.38	21.41	12.25	15.35		1	4	60.0	43.80	53.3	52.37		101.5										33.1	0.4	14.9	0.08	7.77	0.006	1146.4	23.7	2394.8	41.2	2334.7	39.80	27	7.65	43.76	1152.35	1156.54	2221.59	70.84	2394.8	1.09	1.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Tail withdrawal	Animal 15	27.39	14.76	15.81	19.32		1	4	46.2	61.50	76.9	61.53		77.5										33.1	0.4	14.9	0.08	7.77	0.006	1146.4	23.7	2394.8	41.2	2334.7	39.80	27	7.65	43.76	1152.35	1156.54	2221.59	70.84	2394.8	1.09	1.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Tail withdrawal	Animal 16	27.50	7.06	6.45	13.67		1	4	50.0	66.70	66.7	61.13		78.0										33.1	0.4	14.9	0.08	7.77	0.006	1146.4	23.7	2394.8	41.2	2334.7	39.80	27	7.65	43.76	1152.35	1156.54	2221.59	70.84	2394.8	1.09	1.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Tail withdrawal	Animal 17	29.37	25.53	13.58	22.83		1	4	64.3	50.00	57.1	57.13		84.5										33.1	0.4	14.9	0.08	7.77	0.006	1146.4	23.7	2394.8	41.2	2334.7	39.80	27	7.65	43.76	1152.35	1156.54	2221.59	70.84	2394.8	1.09	1.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Tail withdrawal	Animal 1	5.95	7.62	8.04	7.20		1	4	75.0	62.00	53.3	63.43		87.5										33.1	0.4	14.9	0.07	7.36	0.020	3014.6	64.6	2435.7	23.3	2504.8	24.70	27	7.24	119.81	3154.88	3166.37	2364.31	29.31	2435.7	0.45	0.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Tail withdrawal	Animal 2	10.00	7.00	6.83	7.94		1	4	61.5	57.10	69.2	62.60		84.5										33.1	0.4	14.9	0.07	7.36	0.020	3014.6	64.6	2435.7	23.3	2504.8	24.70	27	7.24	119.81	3154.88	3166.37	2364.31	29.31	2435.7	0.45	0.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Tail withdrawal	Animal 3	9.98	10.03	8.00	9.34		1	4	50.0	30.80	38.5	39.77		73.5										33.1	0.4	14.9	0.07	7.36	0.020	3014.6	64.6	2435.7	23.3	2504.8	24.70	27	7.24	119.81	3154.88	3166.37	2364.31	29.31	2435.7	0.45	0.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Tail withdrawal	Animal 4	16.58	11.00	9.00	12.19		2	11	46.7	46.70	33.3	42.23		98.5										33.1	0.4	14.9	0.07	7.36	0.020	3014.6	64.6	2435.7	23.3	2504.8	24.70	27	7.24	119.81	3154.88	3166.37	2364.31	29.31	2435.7	0.45	0.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Tail withdrawal	Animal 5	11.59	13.06	44.86	23.17		2	11	64.3	44.40	53.8	54.17		113.0										33.1	0.4	14.9	0.07	7.36	0.020	3014.6	64.6	2435.7	23.3	2504.8	24.70	27	7.24	119.81	3154.88	3166.37	2364.31	29.31	2435.7	0.45	0.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Tail withdrawal	Animal 6	13.13	32.16	33.55	26.28		1	4	60.0	46.70	53.3	53.33		95.0										33.1	0.4	14.9	0.07	7.36	0.020	3014.6	64.6	2435.7	23.3	2504.8	24.70	27	7.24	119.81	3154.88	3166.37	2364.31	29.31	2435.7	0.45	0.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Tail withdrawal	Animal 7	21.72	24.36	24.97	23.68		1	4	60.0	46.70	60.0	55.57		107.0										33.1	0.4	14.9	0.07	7.36	0.020	3014.6	64.6	2435.7	23.3	2504.8	24.70	27	7.24	119.81	3154.88	3166.37	2364.31	29.31	2435.7	0.45	0.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Tail withdrawal	Animal 8	13.51	13.27	8.23	11.67		1	4	53.3	57.10	50.0	53.47		97.5										33.1	0.4	14.9	0.07	7.36	0.020	3014.6	64.6	2435.7	23.3	2504.8	24.70	27	7.24	119.81	3154.88	3166.37	2364.31	29.31	2435.7	0.45	0.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Tail withdrawal	Animal 9	10.40	6.93	7.35	8.23		1	4	42.9	60.00	64.3	55.73		106.0										33.1	0.4	14.9	0.07	7.36	0.020	3014.6	64.6	2435.7	23.3	2504.8	24.70	27	7.24	119.81	3154.88	3166.37	2364.31	29.31	2435.7	0.45	0.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Tail withdrawal	Animal 10	15.42	24.68	35.78	25.29		1	11	61.5	57.10	57.1	58.57		92.5										33.1	0.4	14.9	0.07	7.36	0.020	3014.6	64.6	2435.7	23.3	2504.8	24.70	27	7.24	119.81	3154.88	3166.37	2364.31	29.31	2435.7	0.45	0.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Tail withdrawal	Animal 11	12.52	13.48	19.51	15.17		1	11	61.5	57.10	57.1	58.57		105.5										33.1	0.4	14.9	0.07	7.36	0.020	3014.6	64.6	2435.7	23.3	2504.8	24.70	27	7.24	119.81	3154.88	3166.37	2364.31	29.31	2435.7	0.45	0.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Tail withdrawal	Animal 12	16.87	25.14	24.78	22.26		1	11	57.1	57.10	57.1	57.10												33.1	0.4	14.9	0.07	7.36	0.020	3014.6	64.6	2435.7	23.3	2504.8	24.70	27	7.24	119.81	3154.88	3166.37	2364.31	29.31	2435.7	0.45	0.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Tail withdrawal	Animal 13	19.36	7.69	6.15	11.07		1	11	50.0	50.00	64.3	54.77												33.1	0.4	14.9	0.07	7.36	0.020	3014.6	64.6	2435.7	23.3	2504.8	24.70	27	7.24	119.81	3154.88	3166.37	2364.31	29.31	2435.7	0.45	0.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Tail withdrawal	Animal 14	7.91	9.15	5.93	7.66		1	4	64.3	50.00	64.3	59.53		75.5										33.1	0.4	14.9	0.07	7.36	0.020	3014.6	64.6	2435.7	23.3	2504.8	24.70	27	7.24	119.81	3154.88	3166.37	2364.31	29.31	2435.7	0.45	0.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Tail withdrawal	Animal 15	12.07	18.53	18.63	16.41		1	4	64.3	60.70	64.3	63.10		91.5										33.1	0.4	14.9	0.07	7.36	0.020	3014.6	64.6	2435.7	23.3	2504.8	24.70	27	7.24	119.81	3154.88	3166.37	2364.31	29.31	2435.7	0.45	0.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Tail withdrawal	Animal 16	6.93	8.68	23.18	12.93		1	4	58.3	69.20	46.2	57.90		66.0										33.1	0.4	14.9	0.07	7.36	0.020	3014.6	64.6	2435.7	23.3	2504.8	24.70	27	7.24	119.81	3154.88	3166.37	2364.31	29.31	2435.7	0.45	0.71
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Acid-base	Animal 1							4							8.095	4.182	0.629							32.3	0.5	15.0	0.04	8.16	0.020	426.1	17.1	2293.0	53.3	2096.0	40.90	27	8.04	15.60	410.24	411.73	1928.88	148.25	2293.0	2.30	3.60
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Acid-base	Animal 2							4						116.5	8.045	4.607	0.781							32.3	0.5	15.0	0.04	8.16	0.020	426.1	17.1	2293.0	53.3	2096.0	40.90	27	8.04	15.60	410.24	411.73	1928.88	148.25	2293.0	2.30	3.60
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Acid-base	Animal 3							4						115.5	7.945	3.836	0.825							32.3	0.5	15.0	0.04	8.16	0.020	426.1	17.1	2293.0	53.3	2096.0	40.90	27	8.04	15.60	410.24	411.73	1928.88	148.25	2293.0	2.30	3.60
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Acid-base	Animal 4							4						96.5	8.085	4.319	0.665							32.3	0.5	15.0	0.04	8.16	0.020	426.1	17.1	2293.0	53.3	2096.0	40.90	27	8.04	15.60	410.24	411.73	1928.88	148.25	2293.0	2.30	3.60
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Acid-base	Animal 5							4						104.5	8.070	4.027	0.643							32.3	0.5	15.0	0.04	8.16	0.020	426.1	17.1	2293.0	53.3	2096.0	40.90	27	8.04	15.60	410.24	411.73	1928.88	148.25	2293.0	2.30	3.60
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Acid-base	Animal 6							4						95.5	8.070	3.295	0.526							32.3	0.5	15.0	0.04	8.16	0.020	426.1	17.1	2293.0	53.3	2096.0	40.90	27	8.04	15.60	410.24	411.73	1928.88	148.25	2293.0	2.30	3.60
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Acid-base	Animal 7							11						129.0	7.940	3.133	0.682							32.3	0.5	15.0	0.04	8.16	0.020	426.1	17.1	2293.0	53.3	2096.0	40.90	27	8.04	15.60	410.24	411.73	1928.88	148.25	2293.0	2.30	3.60
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Acid-base	Animal 8							11						109.5	8.090	3.092	0.471							32.3	0.5	15.0	0.04	8.16	0.020	426.1	17.1	2293.0	53.3	2096.0	40.90	27	8.04	15.60	410.24	411.73	1928.88	148.25	2293.0	2.30	3.60
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Acid-base	Animal 9							11						122.5	8.115	2.852	0.409							32.3	0.5	15.0	0.04	8.16	0.020	426.1	17.1	2293.0	53.3	2096.0	40.90	27	8.04	15.60	410.24	411.73	1928.88	148.25	2293.0	2.30	3.60
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Acid-base	Animal 10							11						101.0	8.125	3.241	0.454							32.3	0.5	15.0	0.04	8.16	0.020	426.1	17.1	2293.0	53.3	2096.0	40.90	27	8.04	15.60	410.24	411.73	1928.88	148.25	2293.0	2.30	3.60
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Acid-base	Animal 11							11						111.5	8.130									32.3	0.5	15.0	0.04	8.16	0.020	426.1	17.1	2293.0	53.3	2096.0	40.90	27	8.04	15.60	410.24	411.73	1928.88	148.25	2293.0	2.30	3.60
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Acid-base	Animal 1							4						91.5	8.065	6.795	1.098							32.2	0.5	15.0	0.05	7.76	0.010	1234.4	58.5	2393.2	91.4	2342.9	80.05	27	7.64	45.34	1191.58	1195.90	2227.63	67.93	2393.2	1.05	1.65
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Acid-base	Animal 2							4						105.5	8.045	8.693	1.474							32.2	0.5	15.0	0.05	7.76	0.010	1234.4	58.5	2393.2	91.4	2342.9	80.05	27	7.64	45.34	1191.58	1195.90	2227.63	67.93	2393.2	1.05	1.65
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Acid-base	Animal 3							4						98.0	8.085	8.448	1.302							32.2	0.5	15.0	0.05	7.76	0.010	1234.4	58.5	2393.2	91.4	2342.9	80.05	27	7.64	45.34	1191.58	1195.90	2227.63	67.93	2393.2	1.05	1.65
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Acid-base	Animal 4							4						92.5	8.095	7.460	1.122							32.2	0.5	15.0	0.05	7.76	0.010	1234.4	58.5	2393.2	91.4	2342.9	80.05	27	7.64	45.34	1191.58	1195.90	2227.63	67.93	2393.2	1.05	1.65
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Acid-base	Animal 5							4						80.0	8.075	8.509	1.343							32.2	0.5	15.0	0.05	7.76	0.010	1234.4	58.5	2393.2	91.4	2342.9	80.05	27	7.64	45.34	1191.58	1195.90	2227.63	67.93	2393.2	1.05	1.65
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Acid-base	Animal 6							4						100.5	8.125	7.118	0.996							32.2	0.5	15.0	0.05	7.76	0.010	1234.4	58.5	2393.2	91.4	2342.9	80.05	27	7.64	45.34	1191.58	1195.90	2227.63	67.93	2393.2	1.05	1.65
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Acid-base	Animal 7							11						106.5	8.055	7.014	1.161							32.2	0.5	15.0	0.05	7.76	0.010	1234.4	58.5	2393.2	91.4	2342.9	80.05	27	7.64	45.34	1191.58	1195.90	2227.63	67.93	2393.2	1.05	1.65
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Acid-base	Animal 8							11						113.5	8.045	7.410	1.256							32.2	0.5	15.0	0.05	7.76	0.010	1234.4	58.5	2393.2	91.4	2342.9	80.05	27	7.64	45.34	1191.58	1195.90	2227.63	67.93	2393.2	1.05	1.65
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Acid-base	Animal 9							11						110.5	8.045	7.073	1.199							32.2	0.5	15.0	0.05	7.76	0.010	1234.4	58.5	2393.2	91.4	2342.9	80.05	27	7.64	45.34	1191.58	1195.90	2227.63	67.93	2393.2	1.05	1.65
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Acid-base	Animal 10							11						103.5	8.070	7.341	1.172							32.2	0.5	15.0	0.05	7.76	0.010	1234.4	58.5	2393.2	91.4	2342.9	80.05	27	7.64	45.34	1191.58	1195.90	2227.63	67.93	2393.2	1.05	1.65
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Acid-base	Animal 11							11						102.0	8.095	6.933	1.043							32.2	0.5	15.0	0.05	7.76	0.010	1234.4	58.5	2393.2	91.4	2342.9	80.05	27	7.64	45.34	1191.58	1195.90	2227.63	67.93	2393.2	1.05	1.65
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Acid-base	Animal 1							4						95.5	7.975	9.734	1.950							32.6	0.5	14.9	0.09	7.41	0.010	2878.1	316.8	2411.2	90.4	2474.4	103.40	27	7.29	106.04	2784.49	2794.63	2333.20	32.01	2411.2	0.50	0.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Acid-base	Animal 2							4						101.5	7.970	7.995	1.620							32.6	0.5	14.9	0.09	7.41	0.010	2878.1	316.8	2411.2	90.4	2474.4	103.40	27	7.29	106.04	2784.49	2794.63	2333.20	32.01	2411.2	0.50	0.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Acid-base	Animal 3							4						106.0	8.015	10.879	1.982							32.6	0.5	14.9	0.09	7.41	0.010	2878.1	316.8	2411.2	90.4	2474.4	103.40	27	7.29	106.04	2784.49	2794.63	2333.20	32.01	2411.2	0.50	0.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Acid-base	Animal 4							4						108.5	8.065	11.507	1.860							32.6	0.5	14.9	0.09	7.41	0.010	2878.1	316.8	2411.2	90.4	2474.4	103.40	27	7.29	106.04	2784.49	2794.63	2333.20	32.01	2411.2	0.50	0.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Acid-base	Animal 5							4						86.0	8.090									32.6	0.5	14.9	0.09	7.41	0.010	2878.1	316.8	2411.2	90.4	2474.4	103.40	27	7.29	106.04	2784.49	2794.63	2333.20	32.01	2411.2	0.50	0.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Acid-base	Animal 6							11						79.0	7.895	11.822	2.861							32.6	0.5	14.9	0.09	7.41	0.010	2878.1	316.8	2411.2	90.4	2474.4	103.40	27	7.29	106.04	2784.49	2794.63	2333.20	32.01	2411.2	0.50	0.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Acid-base	Animal 7							11						125.5	7.975	10.434	2.090							32.6	0.5	14.9	0.09	7.41	0.010	2878.1	316.8	2411.2	90.4	2474.4	103.40	27	7.29	106.04	2784.49	2794.63	2333.20	32.01	2411.2	0.50	0.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Acid-base	Animal 8							11						89.5	8.000	8.895	1.679							32.6	0.5	14.9	0.09	7.41	0.010	2878.1	316.8	2411.2	90.4	2474.4	103.40	27	7.29	106.04	2784.49	2794.63	2333.20	32.01	2411.2	0.50	0.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Acid-base	Animal 9							11						101.5	7.990	9.841	1.902							32.6	0.5	14.9	0.09	7.41	0.010	2878.1	316.8	2411.2	90.4	2474.4	103.40	27	7.29	106.04	2784.49	2794.63	2333.20	32.01	2411.2	0.50	0.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Acid-base	Animal 10							11						102.0	8.025	10.751	1.912							32.6	0.5	14.9	0.09	7.41	0.010	2878.1	316.8	2411.2	90.4	2474.4	103.40	27	7.29	106.04	2784.49	2794.63	2333.20	32.01	2411.2	0.50	0.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Acid-base	Animal 11							11						107.0	8.040	10.503	1.802							32.6	0.5	14.9	0.09	7.41	0.010	2878.1	316.8	2411.2	90.4	2474.4	103.40	27	7.29	106.04	2784.49	2794.63	2333.20	32.01	2411.2	0.50	0.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Righting	Animal 1						1	4						81.0				15.74	37.73	19.34	24.27		1	33.3	0.4	14.9	0.10	8.18	0.010	400.0	24.9	2289.6	56.2	2078.6	58.10	27	8.06	14.54	383.24	384.63	1902.62	156.88	2289.6	2.42	3.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Righting	Animal 2						1	4						77.5				14.62	22.03	12.36	16.34		1	33.3	0.4	14.9	0.10	8.18	0.010	400.0	24.9	2289.6	56.2	2078.6	58.10	27	8.06	14.54	383.24	384.63	1902.62	156.88	2289.6	2.42	3.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Righting	Animal 3						1	4						83.0				15.67	29.42	31.04	25.38		1	33.3	0.4	14.9	0.10	8.18	0.010	400.0	24.9	2289.6	56.2	2078.6	58.10	27	8.06	14.54	383.24	384.63	1902.62	156.88	2289.6	2.42	3.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Righting	Animal 4						1	11						90.5				26.13	15.03	18.38	19.85		2	33.3	0.4	14.9	0.10	8.18	0.010	400.0	24.9	2289.6	56.2	2078.6	58.10	27	8.06	14.54	383.24	384.63	1902.62	156.88	2289.6	2.42	3.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Righting	Animal 5						1	11										18.63	17.44	19.77	18.61		2	33.3	0.4	14.9	0.10	8.18	0.010	400.0	24.9	2289.6	56.2	2078.6	58.10	27	8.06	14.54	383.24	384.63	1902.62	156.88	2289.6	2.42	3.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Righting	Animal 6						1	11						85.5				12.73	21.93	20.75	18.47		2	33.3	0.4	14.9	0.10	8.18	0.010	400.0	24.9	2289.6	56.2	2078.6	58.10	27	8.06	14.54	383.24	384.63	1902.62	156.88	2289.6	2.42	3.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Righting	Animal 7						1	4						105.5				52.08	22.65	49.36	41.36		3	33.3	0.4	14.9	0.10	8.18	0.010	400.0	24.9	2289.6	56.2	2078.6	58.10	27	8.06	14.54	383.24	384.63	1902.62	156.88	2289.6	2.42	3.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Righting	Animal 8						1	4						120.5				29.10	25.29		27.20	video cut before reflex completed	3	33.3	0.4	14.9	0.10	8.18	0.010	400.0	24.9	2289.6	56.2	2078.6	58.10	27	8.06	14.54	383.24	384.63	1902.62	156.88	2289.6	2.42	3.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Righting	Animal 9						1	4						118.0				17.13	14.55	39.70	23.79		3	33.3	0.4	14.9	0.10	8.18	0.010	400.0	24.9	2289.6	56.2	2078.6	58.10	27	8.06	14.54	383.24	384.63	1902.62	156.88	2289.6	2.42	3.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Righting	Animal 10						2	11						79.0				36.31	48.54	40.08	41.64		4	33.3	0.4	14.9	0.10	8.18	0.010	400.0	24.9	2289.6	56.2	2078.6	58.10	27	8.06	14.54	383.24	384.63	1902.62	156.88	2289.6	2.42	3.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Righting	Animal 11						2	11						95.5				27.34	31.18	40.82	33.11		4	33.3	0.4	14.9	0.10	8.18	0.010	400.0	24.9	2289.6	56.2	2078.6	58.10	27	8.06	14.54	383.24	384.63	1902.62	156.88	2289.6	2.42	3.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Righting	Animal 12						2	11						85.5				21.33	16.57	26.90	21.60		4	33.3	0.4	14.9	0.10	8.18	0.010	400.0	24.9	2289.6	56.2	2078.6	58.10	27	8.06	14.54	383.24	384.63	1902.62	156.88	2289.6	2.42	3.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Righting	Animal 13						2	11						88.5				16.68	26.70	28.88	24.09		5	33.3	0.4	14.9	0.10	8.18	0.010	400.0	24.9	2289.6	56.2	2078.6	58.10	27	8.06	14.54	383.24	384.63	1902.62	156.88	2289.6	2.42	3.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Righting	Animal 14						2	11						89.0				53.02	35.51	47.08	45.20		5	33.3	0.4	14.9	0.10	8.18	0.010	400.0	24.9	2289.6	56.2	2078.6	58.10	27	8.06	14.54	383.24	384.63	1902.62	156.88	2289.6	2.42	3.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Righting	Animal 15						2	11						102.5				22.32	27.97	43.24	31.18		5	33.3	0.4	14.9	0.10	8.18	0.010	400.0	24.9	2289.6	56.2	2078.6	58.10	27	8.06	14.54	383.24	384.63	1902.62	156.88	2289.6	2.42	3.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	400	Righting	Animal 16						2	11						80.0				34.06	34.75	21.66	30.16		5	33.3	0.4	14.9	0.10	8.18	0.010	400.0	24.9	2289.6	56.2	2078.6	58.10	27	8.06	14.54	383.24	384.63	1902.62	156.88	2289.6	2.42	3.78
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Righting	Animal 1						1	11						81.5				23.10	14.57	22.61	20.09		6	33.6	0.5	14.8	0.20	7.78	0.007	1166.3	37.7	2417.4	54.1	2356.0	52.30	27	7.66	42.87	1128.76	1132.86	2237.04	73.75	2417.4	1.14	1.77
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Righting	Animal 2						1	11						83.0				13.87	32.34	14.39	20.20		6	33.6	0.5	14.8	0.20	7.78	0.007	1166.3	37.7	2417.4	54.1	2356.0	52.30	27	7.66	42.87	1128.76	1132.86	2237.04	73.75	2417.4	1.14	1.77
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Righting	Animal 3						1	11						84.5				37.53	40.00	28.94	35.49		6	33.6	0.5	14.8	0.20	7.78	0.007	1166.3	37.7	2417.4	54.1	2356.0	52.30	27	7.66	42.87	1128.76	1132.86	2237.04	73.75	2417.4	1.14	1.77
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Righting	Animal 4						1	4						109.5					35.01	33.30	34.16	animal did not complete the full reflex	7	33.6	0.5	14.8	0.20	7.78	0.007	1166.3	37.7	2417.4	54.1	2356.0	52.30	27	7.66	42.87	1128.76	1132.86	2237.04	73.75	2417.4	1.14	1.77
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Righting	Animal 5						1	4						111.0				14.63	27.28	23.33	21.75		7	33.6	0.5	14.8	0.20	7.78	0.007	1166.3	37.7	2417.4	54.1	2356.0	52.30	27	7.66	42.87	1128.76	1132.86	2237.04	73.75	2417.4	1.14	1.77
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Righting	Animal 6						1	4						115.0				24.86	23.92	17.97	22.25		7	33.6	0.5	14.8	0.20	7.78	0.007	1166.3	37.7	2417.4	54.1	2356.0	52.30	27	7.66	42.87	1128.76	1132.86	2237.04	73.75	2417.4	1.14	1.77
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Righting	Animal 7						1	4						123.0				37.54	31.94	33.87	34.45		7	33.6	0.5	14.8	0.20	7.78	0.007	1166.3	37.7	2417.4	54.1	2356.0	52.30	27	7.66	42.87	1128.76	1132.86	2237.04	73.75	2417.4	1.14	1.77
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Righting	Animal 8						1	4						98.5				31.83	33.42	31.80	32.35		7	33.6	0.5	14.8	0.20	7.78	0.007	1166.3	37.7	2417.4	54.1	2356.0	52.30	27	7.66	42.87	1128.76	1132.86	2237.04	73.75	2417.4	1.14	1.77
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Righting	Animal 9						1	4						123.5				35.18	40.25	19.36	31.60		7	33.6	0.5	14.8	0.20	7.78	0.007	1166.3	37.7	2417.4	54.1	2356.0	52.30	27	7.66	42.87	1128.76	1132.86	2237.04	73.75	2417.4	1.14	1.77
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Righting	Animal 10						2	11						82.0				36.81	38.61	48.57	41.33		8	33.6	0.5	14.8	0.20	7.78	0.007	1166.3	37.7	2417.4	54.1	2356.0	52.30	27	7.66	42.87	1128.76	1132.86	2237.04	73.75	2417.4	1.14	1.77
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Righting	Animal 11						2	11						114.5				43.25		50.83	47.04	animal did not complete the full reflex	8	33.6	0.5	14.8	0.20	7.78	0.007	1166.3	37.7	2417.4	54.1	2356.0	52.30	27	7.66	42.87	1128.76	1132.86	2237.04	73.75	2417.4	1.14	1.77
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Righting	Animal 12						2	11						93.5				43.90	21.60	38.38	34.63		8	33.6	0.5	14.8	0.20	7.78	0.007	1166.3	37.7	2417.4	54.1	2356.0	52.30	27	7.66	42.87	1128.76	1132.86	2237.04	73.75	2417.4	1.14	1.77
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Righting	Animal 13						2	11						95.0				22.63	23.33	27.74	24.57		9	33.6	0.5	14.8	0.20	7.78	0.007	1166.3	37.7	2417.4	54.1	2356.0	52.30	27	7.66	42.87	1128.76	1132.86	2237.04	73.75	2417.4	1.14	1.77
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Righting	Animal 14						2	11						98.5				23.16	20.98	17.54	20.56		9	33.6	0.5	14.8	0.20	7.78	0.007	1166.3	37.7	2417.4	54.1	2356.0	52.30	27	7.66	42.87	1128.76	1132.86	2237.04	73.75	2417.4	1.14	1.77
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Righting	Animal 15						2	11						87.0				19.53	23.68	23.47	22.23		9	33.6	0.5	14.8	0.20	7.78	0.007	1166.3	37.7	2417.4	54.1	2356.0	52.30	27	7.66	42.87	1128.76	1132.86	2237.04	73.75	2417.4	1.14	1.77
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	1200	Righting	Animal 16						2	11						93.0				19.74	14.25	22.00	18.66		9	33.6	0.5	14.8	0.20	7.78	0.007	1166.3	37.7	2417.4	54.1	2356.0	52.30	27	7.66	42.87	1128.76	1132.86	2237.04	73.75	2417.4	1.14	1.77
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Righting	Animal 1						1	4						76.5				11.59	16.35	21.37	16.44		10	33.1	0.1	14.9	0.10	7.38	0.005	2927.1	40.7	2423.5	22.1	2487.9	23.30	27	7.26	113.67	2993.38	3004.28	2349.14	30.49	2423.5	0.47	0.74
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Righting	Animal 2						1	4						90.0				10.85	15.64		13.25	video cut before reflex completed	10	33.1	0.1	14.9	0.10	7.38	0.005	2927.1	40.7	2423.5	22.1	2487.9	23.30	27	7.26	113.67	2993.38	3004.28	2349.14	30.49	2423.5	0.47	0.74
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Righting	Animal 3						1	4						93.5				34.20	19.33	20.98	26.77		10	33.1	0.1	14.9	0.10	7.38	0.005	2927.1	40.7	2423.5	22.1	2487.9	23.30	27	7.26	113.67	2993.38	3004.28	2349.14	30.49	2423.5	0.47	0.74
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Righting	Animal 4						1	11						91.5				23.67	24.98		24.33	video cut before reflex completed	11	33.1	0.1	14.9	0.10	7.38	0.005	2927.1	40.7	2423.5	22.1	2487.9	23.30	27	7.26	113.67	2993.38	3004.28	2349.14	30.49	2423.5	0.47	0.74
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Righting	Animal 5						1	11						82.5				18.45	22.80		20.63	video cut before reflex completed	11	33.1	0.1	14.9	0.10	7.38	0.005	2927.1	40.7	2423.5	22.1	2487.9	23.30	27	7.26	113.67	2993.38	3004.28	2349.14	30.49	2423.5	0.47	0.74
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Righting	Animal 6						1	11						83.5				18.24	21.86	19.11	19.74		11	33.1	0.1	14.9	0.10	7.38	0.005	2927.1	40.7	2423.5	22.1	2487.9	23.30	27	7.26	113.67	2993.38	3004.28	2349.14	30.49	2423.5	0.47	0.74
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Righting	Animal 7						1	4						92.0				39.15	40.14	25.03	34.77		12	33.1	0.1	14.9	0.10	7.38	0.005	2927.1	40.7	2423.5	22.1	2487.9	23.30	27	7.26	113.67	2993.38	3004.28	2349.14	30.49	2423.5	0.47	0.74
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Righting	Animal 8						1	4						89.5				35.91	33.51	42.28	39.10		12	33.1	0.1	14.9	0.10	7.38	0.005	2927.1	40.7	2423.5	22.1	2487.9	23.30	27	7.26	113.67	2993.38	3004.28	2349.14	30.49	2423.5	0.47	0.74
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Righting	Animal 9						1	4						131.5				28.93	20.78		24.86	animal did not complete the full reflex	12	33.1	0.1	14.9	0.10	7.38	0.005	2927.1	40.7	2423.5	22.1	2487.9	23.30	27	7.26	113.67	2993.38	3004.28	2349.14	30.49	2423.5	0.47	0.74
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Righting	Animal 10						2	11						96.5				28.95	21.00		24.98	animal did not complete the full reflex	13	33.1	0.1	14.9	0.10	7.38	0.005	2927.1	40.7	2423.5	22.1	2487.9	23.30	27	7.26	113.67	2993.38	3004.28	2349.14	30.49	2423.5	0.47	0.74
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Righting	Animal 11						2	11						77.0				13.93	25.08	12.42	17.14		13	33.1	0.1	14.9	0.10	7.38	0.005	2927.1	40.7	2423.5	22.1	2487.9	23.30	27	7.26	113.67	2993.38	3004.28	2349.14	30.49	2423.5	0.47	0.74
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Righting	Animal 12						2	11						73.5				22.71	25.87	12.68	20.42		13	33.1	0.1	14.9	0.10	7.38	0.005	2927.1	40.7	2423.5	22.1	2487.9	23.30	27	7.26	113.67	2993.38	3004.28	2349.14	30.49	2423.5	0.47	0.74
laboratory	Aplysia californica (mollusk)	240765	marinespecies.org	3000	Righting	Animal 13						2	11						103.0				43.75	41.18		42.47	animal did not complete the full reflex	13	33.1	0.1	14.9	0.10	7.38	0.005	2927.1	40.7	2423.5	22.1	2487.9	23.30	27	7.26	113.67	2993.38	3004.28	2349.14	30.49	2423.5	0.47	0.74