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Mittermayer, Felix H; Stiasny, Martina H; Clemmesen, Catriona; Bayer, Till; Puvanendran, Velmurugu; Chierici, Melissa; Jentoft, Sissel; Reusch, Thorsten B H (2019): Seawater carbonate chemistry and dry weight of 5 and 15 days-post-hatch Atlantic cod larvae [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.911498

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Abstract:
Ocean acidification (OA), a direct consequence of increasing atmospheric CO2 concentration dissolving in ocean waters, is impacting many fish species. Little is known about the molecular mechanisms underlying the observed physiological impacts in fish. We used RNAseq to characterize the transcriptome of 3 different larval stages of Atlantic cod (Gadus morhua) exposed to simulated OA at levels (1179 µatm CO2) representing end-of-century predictions compared to controls (503 µatm CO2), which were shown to induce tissue damage and elevated mortality in G. morhua. Only few genes were differentially expressed in 6 and 13 days-post-hatching (dph) (3 and 16 genes, respectively), during a period when maximal mortality as a response to elevated pCO2 occurred. At 36 dph, 1413 genes were differentially expressed, most likely caused by developmental asynchrony between the treatment groups, with individuals under OA growing faster. A target gene analysis revealed only few genes of the universal and well-defined cellular stress response to be differentially expressed. We thus suggest that predicted ocean acidification levels constitute a “stealth stress” for early Atlantic cod larvae, with a rapid breakdown of cellular homeostasis leading to organismal death that was missed even with an 8-fold replication implemented in this study.
Keyword(s):
Animalia; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gadus morhua; Growth/Morphology; Laboratory experiment; Nekton; North Atlantic; Pelagos; Polar; Single species
Related to:
Mittermayer, Felix H; Stiasny, Martina H; Clemmesen, Catriona; Bayer, Till; Puvanendran, Velmurugu; Chierici, Melissa; Jentoft, Sissel; Reusch, Thorsten B H (2019): Transcriptome profiling reveals exposure to predicted end-of-century ocean acidification as a stealth stressor for Atlantic cod larvae. Scientific Reports, 9(1), https://doi.org/10.1038/s41598-019-52628-1
Stiasny, Martina H; Mittermayer, Felix H; Sswat, Michael; Voss, Rüdiger; Jutfelt, Fredrik; Chierici, Melissa; Puvanendran, Velmurugu; Mortensen, Atle; Reusch, Thorsten B H; Clemmesen, Catriona (2016): Survival of Atlantic cod larvae from the Barents Sea and the Western Baltic sea due to ocean acidification from two experiments : CO2 data [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.858615
Original version:
Mittermayer, Felix H; Stiasny, Martina H; Clemmesen, Catriona; Bayer, Till; Puvanendran, Velmurugu; Chierici, Melissa; Jentoft, Sissel; Reusch, Thorsten B H (2018): Dry weight of 5 and 15 days-post-hatch Atlantic cod larvae in response to predicted end-of-century ocean acidification [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.884548
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb
Coverage:
Latitude: 70.250000 * Longitude: 19.000000
Date/Time Start: 2014-03-01T00:00:00 * Date/Time End: 2014-03-31T00:00:00
Event(s):
Barents_Sea * Latitude: 70.250000 * Longitude: 19.000000 * Date/Time Start: 2014-03-01T00:00:00 * Date/Time End: 2014-03-31T00:00:00 * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-01-28.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeMittermayer, Felix Hstudy
2SpeciesSpeciesMittermayer, Felix H
3Registration number of speciesReg spec noMittermayer, Felix H
4Uniform resource locator/link to referenceURL refMittermayer, Felix HWoRMS Aphia ID
5Sample, optional label/labor noLab noMittermayer, Felix HRunning ID number for individual identification of each analysed larvae
6AgeAgedaysMittermayer, Felix HAge of larvae in days-post-hatch. Either 5 or 15 dph
7IdentificationIDMittermayer, Felix HTank number. Approximately 10 Larvae were analysed from each tank.
8TreatmentTreatMittermayer, Felix HLarval pCO2 treatment
9Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmMittermayer, Felix HTreatment
10Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Mittermayer, Felix H
11Larvae, dry massLarvae dmgMittermayer, Felix Hafter 16h freeze drying
12Temperature, waterTemp°CMittermayer, Felix H
13SalinitySalMittermayer, Felix H
14Alkalinity, totalATµmol/kgMittermayer, Felix H
15Carbon, inorganic, dissolvedDICµmol/kgMittermayer, Felix H
16Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
17pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
18Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
19Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
20Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
21Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
22Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
23Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
24Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2496 data points

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