Citation:

PANGAEA.911498

Name: Seawater carbonate chemistry and dry weight of 5 and 15 days-post-hatch Atlantic cod larvae
Published: 2019
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gadus morhua; Growth/Morphology; Laboratory experiment; Nekton; North Atlantic; Pelagos; Polar; Single species

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Abstract:

Ocean acidification (OA), a direct consequence of increasing atmospheric CO2 concentration dissolving in ocean waters, is impacting many fish species. Little is known about the molecular mechanisms underlying the observed physiological impacts in fish. We used RNAseq to characterize the transcriptome of 3 different larval stages of Atlantic cod (Gadus morhua) exposed to simulated OA at levels (1179 µatm CO2) representing end-of-century predictions compared to controls (503 µatm CO2), which were shown to induce tissue damage and elevated mortality in G. morhua. Only few genes were differentially expressed in 6 and 13 days-post-hatching (dph) (3 and 16 genes, respectively), during a period when maximal mortality as a response to elevated pCO2 occurred. At 36 dph, 1413 genes were differentially expressed, most likely caused by developmental asynchrony between the treatment groups, with individuals under OA growing faster. A target gene analysis revealed only few genes of the universal and well-defined cellular stress response to be differentially expressed. We thus suggest that predicted ocean acidification levels constitute a “stealth stress” for early Atlantic cod larvae, with a rapid breakdown of cellular homeostasis leading to organismal death that was missed even with an 8-fold replication implemented in this study.

Keyword(s):

Animalia; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gadus morhua; Growth/Morphology; Laboratory experiment; Nekton; North Atlantic; Pelagos; Polar; Single species

Related to:

Mittermayer, Felix H; Stiasny, Martina H; Clemmesen, Catriona; Bayer, Till; Puvanendran, Velmurugu; Chierici, Melissa; Jentoft, Sissel; Reusch, Thorsten B H (2019): Transcriptome profiling reveals exposure to predicted end-of-century ocean acidification as a stealth stressor for Atlantic cod larvae. Scientific Reports, 9(1), https://doi.org/10.1038/s41598-019-52628-1

Stiasny, Martina H; Mittermayer, Felix H; Sswat, Michael; Voss, Rüdiger; Jutfelt, Fredrik; Chierici, Melissa; Puvanendran, Velmurugu; Mortensen, Atle; Reusch, Thorsten B H; Clemmesen, Catriona (2016): Survival of Atlantic cod larvae from the Barents Sea and the Western Baltic sea due to ocean acidification from two experiments : CO2 data. PANGAEA, https://doi.org/10.1594/PANGAEA.858615

Original version:

Mittermayer, Felix H; Stiasny, Martina H; Clemmesen, Catriona; Bayer, Till; Puvanendran, Velmurugu; Chierici, Melissa; Jentoft, Sissel; Reusch, Thorsten B H (2018): Dry weight of 5 and 15 days-post-hatch Atlantic cod larvae in response to predicted end-of-century ocean acidification. PANGAEA, https://doi.org/10.1594/PANGAEA.884548

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 70.250000 * Longitude: 19.000000

Date/Time Start: 2014-03-01T00:00:00 * Date/Time End: 2014-03-31T00:00:00

Event(s):

Barents_Sea * Latitude: 70.250000 * Longitude: 19.000000 * Date/Time Start: 2014-03-01T00:00:00 * Date/Time End: 2014-03-31T00:00:00 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-01-28.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Mittermayer, Felix H		study
2	Species	Species		Mittermayer, Felix H
3	Registration number of species	Reg spec no		Mittermayer, Felix H
4	Uniform resource locator/link to reference	URL ref		Mittermayer, Felix H		WoRMS Aphia ID
5	Sample, optional label/labor no	Lab no		Mittermayer, Felix H		Running ID number for individual identification of each analysed larvae
6	Age	Age	days	Mittermayer, Felix H		Age of larvae in days-post-hatch. Either 5 or 15 dph
7	Identification	ID		Mittermayer, Felix H		Tank number. Approximately 10 Larvae were analysed from each tank.
8	Treatment	Treat		Mittermayer, Felix H		Larval pCO2 treatment
9	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Mittermayer, Felix H		Treatment
10	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Mittermayer, Felix H
11	Larvae, dry mass	Larvae dm	g	Mittermayer, Felix H		after 16h freeze drying
12	Temperature, water	Temp	°C	Mittermayer, Felix H
13	Salinity	Sal		Mittermayer, Felix H
14	Alkalinity, total	AT	µmol/kg	Mittermayer, Felix H
15	Carbon, inorganic, dissolved	DIC	µmol/kg	Mittermayer, Felix H
16	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
17	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
18	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
19	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
20	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
21	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
22	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
23	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
24	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2496 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Lab no (Running ID number for individ...)	6 Age [days] (Age of larvae in days-post-ha...)	7 ID (Tank number. Approximately 10...)	8 Treat (Larval pCO2 treatment)	9 pCO2water_SST_wet [µatm] (Treatment)	10 pCO2 std dev [±]	11 Larvae dm [g] (after 16h freeze drying)	12 Temp [°C]	13 Sal	14 AT [µmol/kg]	15 DIC [µmol/kg]	16 CSC flag	17 pH (total scale)	18 CO2 [µmol/kg]	19 fCO2water_SST_wet [µatm]	20 pCO2water_SST_wet [µatm]	21 [HCO3]- [µmol/kg]	22 [CO3]2- [µmol/kg]	23 Omega Arg	24 Omega Cal
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S191	15	127	Ambient pCO2	503	89	0.0000898	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S192	15	127	Ambient pCO2	503	89	0.0001198	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S193	15	127	Ambient pCO2	503	89	0.0001070	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S194	15	127	Ambient pCO2	503	89	0.0001093	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S195	15	127	Ambient pCO2	503	89	0.0001018	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S196	15	127	Ambient pCO2	503	89	0.0000990	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S197	15	127	Ambient pCO2	503	89	0.0001388	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S198	15	127	Ambient pCO2	503	89	0.0000984	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S199	15	127	Ambient pCO2	503	89	0.0001067	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S200	15	127	Ambient pCO2	503	89	0.0000982	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S211	15	121	Ambient pCO2	503	89	0.0000621	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S212	15	121	Ambient pCO2	503	89	0.0000778	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S213	15	121	Ambient pCO2	503	89	0.0001021	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S214	15	121	Ambient pCO2	503	89	0.0000824	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S215	15	121	Ambient pCO2	503	89	0.0000875	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S216	15	121	Ambient pCO2	503	89	0.0001070	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S217	15	121	Ambient pCO2	503	89	0.0000778	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S218	15	121	Ambient pCO2	503	89	0.0000877	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S219	15	121	Ambient pCO2	503	89	0.0000675	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S220	15	121	Ambient pCO2	503	89	0.0001125	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S281	15	148	High pCO2	1179	87	0.0000634	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S282	15	148	High pCO2	1179	87	0.0001373	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S283	15	148	High pCO2	1179	87	0.0001497	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S284	15	148	High pCO2	1179	87	0.0000865	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S285	15	148	High pCO2	1179	87	0.0000771	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S286	15	148	High pCO2	1179	87	0.0000927	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S287	15	148	High pCO2	1179	87	0.0000800	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S288	15	148	High pCO2	1179	87	0.0000833	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S289	15	148	High pCO2	1179	87	0.0001720	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S290	15	148	High pCO2	1179	87	0.0000677	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S301	5	116	Ambient pCO2	503	89	0.0000413	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S303	5	116	Ambient pCO2	503	89	0.0000483	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S304	5	116	Ambient pCO2	503	89	0.0000902	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S305	5	116	Ambient pCO2	503	89	0.0001026	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S306	5	116	Ambient pCO2	503	89	0.0000870	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S307	5	116	Ambient pCO2	503	89	0.0001020	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S308	5	116	Ambient pCO2	503	89	0.0000498	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S309	5	116	Ambient pCO2	503	89	0.0000543	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S310	5	116	Ambient pCO2	503	89	0.0000295	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S311	5	151	High pCO2	1179	87	0.0000408	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S312	5	151	High pCO2	1179	87	0.0000320	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S313	5	151	High pCO2	1179	87	0.0000293	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S314	5	151	High pCO2	1179	87	0.0000625	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S315	5	151	High pCO2	1179	87	0.0000308	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S317	5	151	High pCO2	1179	87	0.0000745	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S318	5	151	High pCO2	1179	87	0.0000348	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S319	5	151	High pCO2	1179	87	0.0000357	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S320	5	151	High pCO2	1179	87	0.0000980	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S361	5	144	High pCO2	1179	87	0.0000801	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S362	5	144	High pCO2	1179	87	0.0000409	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S365	5	144	High pCO2	1179	87	0.0000475	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S366	5	144	High pCO2	1179	87	0.0000451	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S367	5	144	High pCO2	1179	87	0.0000850	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S368	5	144	High pCO2	1179	87	0.0000431	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S369	5	144	High pCO2	1179	87	0.0000522	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S370	5	144	High pCO2	1179	87	0.0000267	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S401	5	149	High pCO2	1179	87	0.0000444	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S402	5	149	High pCO2	1179	87	0.0000306	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S403	5	149	High pCO2	1179	87	0.0000507	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S404	5	149	High pCO2	1179	87	0.0000060	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S405	5	149	High pCO2	1179	87	0.0000782	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S406	5	149	High pCO2	1179	87	0.0000446	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S407	5	149	High pCO2	1179	87	0.0000412	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S408	5	149	High pCO2	1179	87	0.0000505	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S409	5	149	High pCO2	1179	87	0.0000459	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S410	5	124	Ambient pCO2	503	89	0.0000806	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S411	5	124	Ambient pCO2	503	89	0.0000542	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S412	5	124	Ambient pCO2	503	89	0.0000429	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S413	5	124	Ambient pCO2	503	89	0.0000935	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S414	5	124	Ambient pCO2	503	89	0.0000433	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S415	5	124	Ambient pCO2	503	89	0.0000535	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S416	5	124	Ambient pCO2	503	89	0.0000518	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S417	5	124	Ambient pCO2	503	89	0.0000425	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S418	5	124	Ambient pCO2	503	89	0.0000791	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S419	5	124	Ambient pCO2	503	89	0.0000501	10	33.8	2278.55	2143.41	15	7.94	22.96	519.56	521.57	2014.41	106.04	1.62	2.54
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S430	5	113	High pCO2	1179	87	0.0000510	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S431	5	113	High pCO2	1179	87	0.0000425	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S432	5	113	High pCO2	1179	87	0.0000812	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S433	5	113	High pCO2	1179	87	0.0000392	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S434	5	113	High pCO2	1179	87	0.0000434	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S435	5	113	High pCO2	1179	87	0.0000670	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S436	5	113	High pCO2	1179	87	0.0000107	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S437	5	113	High pCO2	1179	87	0.0000536	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	S439	5	113	High pCO2	1179	87	0.0000454	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C1	15	137	High pCO2	1179	87	0.0001152	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C2	15	137	High pCO2	1179	87	0.0001458	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C3	15	137	High pCO2	1179	87	0.0001531	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C4	15	137	High pCO2	1179	87	0.0001171	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C5	15	137	High pCO2	1179	87	0.0001225	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C6	15	137	High pCO2	1179	87	0.0001447	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C7	15	137	High pCO2	1179	87	0.0001461	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C8	15	137	High pCO2	1179	87	0.0000996	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C9	15	137	High pCO2	1179	87	0.0001193	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C10	15	137	High pCO2	1179	87	0.0001167	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C21	15	143	High pCO2	1179	87	0.0000963	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C22	15	143	High pCO2	1179	87	0.0001575	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C23	15	143	High pCO2	1179	87	0.0000966	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C24	15	143	High pCO2	1179	87	0.0001294	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C25	15	143	High pCO2	1179	87	0.0000989	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C26	15	143	High pCO2	1179	87	0.0001402	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C27	15	143	High pCO2	1179	87	0.0001093	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C28	15	143	High pCO2	1179	87	0.0001249	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C29	15	143	High pCO2	1179	87	0.0001247	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23
laboratory	Gadus morhua (fish)	126436	marinespecies.org	C30	15	143	High pCO2	1179	87	0.0000770	10	33.8	2284.83	2262.73	15	7.60	54.26	1228.02	1232.77	2157.27	51.20	0.78	1.23