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Vad, Johanne; Durán Suja, Laura (2020): Hydrocarbon data of the cold-water sponge Halichondria panicea exposure experiment to Benzo-A-Pyrene [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.911443

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Published: 2020-01-28DOI registered: 2020-06-17

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Abstract:
In this study, inter-tidal cold-water sponge Halichondria panicea samples were exposed to Benzo-A-pyrene for 48h (accumulation phase) and then left to recover for 13 days in clean seawater (depuration phase). Tissue samples were collected at multiple time points during the accumulation phase to conduct qPCR targeting several genes of interest. Tissue samples were also collected during the accumulation and depuration phases to determine concentration of hydrocarbon in the sponge tissue. The files provided here detail (1) the hydrocarbon data determined by GC/MS analysis and (2) the sponge log fold change data for four genes of interest derived by qPCR.
Keyword(s):
Benzo-A-Pyrene; Poly Aromatic Hydrocarbons; qPCR; sponge
Funding:
Horizon 2020 (H2020), grant/award no. 678760: A Trans-Atlantic assessment and deep-water ecosystem-based spatial management plan for Europe
Comment:
Entries starting with "<" means that the concentration was below the limit of detection given after the sign "<".
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Sample IDSample IDVad, Johanne
2ExperimentExpVad, JohannePhase of experiment
3StatusStatusVad, JohanneState
4TreatmentTreatVad, Johanne
5ReplicatesRepl#Vad, Johanne
6DurationDurationVad, JohanneTime
7NaphthaleneNaphthµg/gVad, JohanneGas chromatography - Mass spectrometry (GC-MS)
8AcenaphthyleneAcenaphthyleneµg/gVad, JohanneGas chromatography - Mass spectrometry (GC-MS)
9AcenaphtheneAcenaphtheneµg/gVad, JohanneGas chromatography - Mass spectrometry (GC-MS)
10FluoreneFluoreneµg/gVad, JohanneGas chromatography - Mass spectrometry (GC-MS)
11PhenanthrenePhenanthreneµg/gVad, JohanneGas chromatography - Mass spectrometry (GC-MS)
12AnthraceneAnthraceneµg/gVad, JohanneGas chromatography - Mass spectrometry (GC-MS)
13FluorantheneFluorantheneµg/gVad, JohanneGas chromatography - Mass spectrometry (GC-MS)
14PyrenePyreneµg/gVad, JohanneGas chromatography - Mass spectrometry (GC-MS)
15Benzo(a)anthraceneBenzo(a)anthraceneµg/gVad, JohanneGas chromatography - Mass spectrometry (GC-MS)
16ChryseneChryseneµg/gVad, JohanneGas chromatography - Mass spectrometry (GC-MS)
17Benzo(b)fluorantheneBenzo(b)fluorantheneµg/gVad, JohanneGas chromatography - Mass spectrometry (GC-MS)
18Benzo(k)fluorantheneBenzo(k)fluorantheneµg/gVad, JohanneGas chromatography - Mass spectrometry (GC-MS)
19Benzo(a)pyreneBenzo(a)pyreneµg/gVad, JohanneGas chromatography - Mass spectrometry (GC-MS)
20Indeno(1,2,3-cd)pyreneIndeno(1,2,3-cd)pyµg/gVad, JohanneGas chromatography - Mass spectrometry (GC-MS)
21Dibenzo(a,h)anthraceneDibenzo(a,h)anthµg/gVad, JohanneGas chromatography - Mass spectrometry (GC-MS)
22Benzo(g,h,i)peryleneBenzo(g,h,i)peryleneµg/gVad, JohanneGas chromatography - Mass spectrometry (GC-MS)
23Polycyclic aromatic hydrocarbonsPAHµg/gVad, JohanneGas chromatography - Mass spectrometry (GC-MS)
Size:
966 data points

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