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Vad, Johanne; Durán Suja, Laura (2020): Results of the cold-water sponge Halichondria panicea exposure experiment to Benzo-A-Pyrene [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.911441

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Published: 2020-01-28DOI registered: 2020-06-17

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Abstract:
In this study, inter-tidal cold-water sponge Halichondria panicea samples were exposed to Benzo-A-pyrene for 48h (accumulation phase) and then left to recover for 13 days in clean seawater (depuration phase). Tissue samples were collected at multiple time points during the accumulation phase to conduct qPCR targeting several genes of interest. Tissue samples were also collected during the accumulation and depuration phases to determine concentration of hydrocarbon in the sponge tissue. The files provided here detail (1) the hydrocarbon data determined by GC/MS analysis and (2) the sponge log fold change data for four genes of interest derived by qPCR.
Keyword(s):
Benzo-A-Pyrene; Poly Aromatic Hydrocarbons; qPCR; sponge
Funding:
Horizon 2020 of the European Commission (H2020), grant/award no. 678760: A Trans-Atlantic assessment and deep-water ecosystem-based spatial management plan for Europe
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Sample IDSample IDVad, Johanne
2TreatmentTreatVad, Johanne
3Time in hoursTime hhVad, Johanne
4Halichondria panicea, heat shock protein 70, log fold changeH. panicea Hsp70 logFCVad, JohanneCalculated
5Halichondria panicea, heat shock protein 90, log fold changeH. panicea Hsp90 logFCVad, JohanneCalculated
6Halichondria panicea, cyclophilinH. panicea cyclophilinVad, JohanneCalculated
7Halichondria panicea, cytochrome b5H. panicea cytochrome b5Vad, JohanneCalculated
Size:
429 data points

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