Citation:

PANGAEA.911441

Name: Results of the cold-water sponge Halichondria panicea exposure experiment to Benzo-A-Pyrene
Published: 2020-01-28
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Benzo-A-Pyrene; Poly Aromatic Hydrocarbons; qPCR; sponge

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Published: 2020-01-28 • DOI registered: 2020-06-17

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Abstract:

In this study, inter-tidal cold-water sponge Halichondria panicea samples were exposed to Benzo-A-pyrene for 48h (accumulation phase) and then left to recover for 13 days in clean seawater (depuration phase). Tissue samples were collected at multiple time points during the accumulation phase to conduct qPCR targeting several genes of interest. Tissue samples were also collected during the accumulation and depuration phases to determine concentration of hydrocarbon in the sponge tissue. The files provided here detail (1) the hydrocarbon data determined by GC/MS analysis and (2) the sponge log fold change data for four genes of interest derived by qPCR.

Keyword(s):

Benzo-A-Pyrene; Poly Aromatic Hydrocarbons; qPCR; sponge

Project(s):

A Trans-Atlantic assessment and deep-water ecosystem-based spatial management plan for Europe (ATLAS)

Funding:

Horizon 2020 of the European Commission (H2020), grant/award no. 678760 : A Trans-Atlantic assessment and deep-water ecosystem-based spatial management plan for Europe

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device
1	Sample ID	Sample ID		Vad, Johanne
2	Treatment	Treat		Vad, Johanne
3	Time in hours	Time h	h	Vad, Johanne
4	Halichondria panicea, heat shock protein 70, log fold change	H. panicea Hsp70 logFC		Vad, Johanne	Calculated
5	Halichondria panicea, heat shock protein 90, log fold change	H. panicea Hsp90 logFC		Vad, Johanne	Calculated
6	Halichondria panicea, cyclophilin	H. panicea cyclophilin		Vad, Johanne	Calculated
7	Halichondria panicea, cytochrome b5	H. panicea cytochrome b5		Vad, Johanne	Calculated

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Size:

429 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Sample ID	2 Treat	3 Time h [h]	4 H. panicea Hsp70 logFC	5 H. panicea Hsp90 logFC	6 H. panicea cyclophilin	7 H. panicea cytochrome b5
1	Control	0	1	0	0	0
2	Control	0	1	2	1	2
3	Control	0	1	2	1	1
4	Control	0	2	4	1
5	Control	0	0	0	1
6	Control	0	4	2	2	2
1	Control	1	0	0	0	0
2	Control	1	3	3	1	2
4	Control	1	1	1	3	1
5	Control	1	1	2	2	1
6	Control	1	1	1		1
1	Control	3	2	5	2	1
2	Control	3	1	1	0	1
3	Control	3	1	1	2	2
4	Control	3	3	2	2
5	Control	3	2	3	1
6	Control	3	0	0	0	0
1	Control	6	0	0	0	1
2	Control	6	0	1	2	0
3	Control	6	1	1	0
4	Control	6	4	1	3
5	Control	6	3	4	1	2
6	Control	6	1	1	1	2
1	Control	24	1	2	2	2
2	Control	24	1	2	1
3	Control	24	1	0	1
4	Control	24	2	0	2	0
5	Control	24	1	1	1	2
6	Control	24	1	2	0	1
2	Control	48	1	1	0
3	Control	48	0	0	0	1
4	Control	48	2	0	2	2
5	Control	48	2	4	2
6	Control	48	1	3	2	1
4	BaP	0	2	1	2	2
5	BaP	0	1	0	0	1
6	BaP	0	1	0	0	1
1	BaP	1	1	1	20
2	BaP	1	2	1	21	1
3	BaP	1	3	12	3
4	BaP	1	2	21	24	1
5	BaP	1	6	18	11	1
6	BaP	1	19	6		12
1	BaP	3	17	23	17	11
2	BaP	3	4	5	27	4
3	BaP	3	10	10	29	4
4	BaP	3	20	27		24
5	BaP	3	19	11	3
6	BaP	3	8	14	10
1	BaP	6	1	20	2	5
2	BaP	6	1	3		0
3	BaP	6	1	1	1	1
4	BaP	6	1	0	11	5
5	BaP	6	1	1	2	4
6	BaP	6	0	8	0	3
2	BaP	24	4	11	3	4
3	BaP	24	1	3	3	6
4	BaP	24	0	1	4	0
5	BaP	24	1	2	1	1
1	BaP	48	1	9	3	1
2	BaP	48	0	1		1
3	BaP	48	1	1	9	4
5	BaP	48	0	1	8	5
6	BaP	48	0	0	13	1