Citation:

PANGAEA.908130

Name: Prokaryotic cell abundance and biomass measured during Maria S. Merian cruise MSM29 and Polarstern cruise PS85 to the Fram Strait
Creator: Christina Bienhold
Published: 2019-11-06
License: https://creativecommons.org/licenses/by/4.0/
Keywords: AODC; bacterial abundance; bacterial biomass; cell counts; deep sea sediment; Fram Strait

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Published: 2019-11-06 • DOI registered: 2020-07-16

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Abstract:

Prokaryotic cell abundances and biomass were determined in deep-sea sediment samples collected at the deep-sea LTER HAUSGARTEN, located in the Fram Strait. Sampling was performed during two cruises, i.e. RV Maria S. Merian MSM 29 in summer 2013 (23 June-12 July) and RV Polarstern PS85 in summer 2014 (6 June-3 July). Undisturbed sediment cores were collected with a TV-guided multicorer. Prokaryotic cell abundances were determined using acridine orange direct counts, following the procedure described in (Hoffmann et al., 2017; doi:10.3389/fmicb.2017.00266). For each sample two replicate filters were counted. Cell volumes were determined using a stage micrometer and converted to biomass using a conversion factor of 3 x 10^-13 g C /µm³ (Børsheim, K. Y., Bratbak, G., and Heldal, M. (1990). Enumeration and biomass estimation of planktonic bacteria and viruses by transmission electron microscopy. Applied and Environmental Microbiology 56, 352-356).

Keyword(s):

AODC; bacterial abundance; bacterial biomass; cell counts; deep sea sediment; Fram Strait

Supplement to:

Rossel, Pamela E; Bienhold, Christina; Hehemann, Laura; Dittmar, Thorsten; Boetius, Antje (2020): Molecular Composition of Dissolved Organic Matter in Sediment Porewater of the Arctic Deep-Sea Observatory HAUSGARTEN (Fram Strait). Frontiers in Marine Science, 7, 428, https://doi.org/10.3389/fmars.2020.00428

Project(s):

Assessment of bacterial life and matter cycling in deep-sea surface sediments (ABYSS)

Funding:

Seventh Framework Programme (FP7), grant/award no. 294757 : Assessment of bacterial life and matter cycling in deep-sea surface sediments

Coverage:

Median Latitude: 79.053645 * Median Longitude: 3.427493 * South-bound Latitude: 78.505670 * West-bound Longitude: -5.290500 * North-bound Latitude: 79.757830 * East-bound Longitude: 7.003500

Date/Time Start: 2013-06-26T01:11:00 * Date/Time End: 2014-06-25T16:59:00

Minimum DEPTH, sediment/rock: 0.005 m * Maximum DEPTH, sediment/rock: 0.005 m

Event(s):

MSM29_424-3 (HG_II) * Latitude Start: 79.132330 * Longitude Start: 4.911500 * Latitude End: 79.132000 * Longitude End: 4.912000 * Date/Time Start: 2013-06-26T01:11:00 * Date/Time End: 2013-06-26T02:19:59 * Elevation Start: -1506.3 m * Elevation End: -1506.3 m * Location: North Greenland Sea * Campaign: (HAUSGARTEN 2013) * Basis: * Method/Device: Multicorer with television (TVMUC) * Comment: rope length max. 1485m / FR2

MSM29_425-3 (HG_I) * Latitude: 79.133330 * Longitude: 6.092500 * Date/Time: 2013-06-26T07:10:00 * Elevation: -1253.8 m * Location: North Greenland Sea * Campaign: (HAUSGARTEN 2013) * Basis: * Method/Device: MultiCorer (MUC) * Comment: rope length max. 1240m / FR2/2

MSM29_427-2 (KH) * Latitude Start: 79.030000 * Longitude Start: 7.003500 * Latitude End: 79.030170 * Longitude End: 7.002670 * Date/Time Start: 2013-06-27T01:02:00 * Date/Time End: 2013-06-27T01:58:59 * Elevation Start: -1280.8 m * Elevation End: -1279.4 m * Location: North Greenland Sea * Campaign: (HAUSGARTEN 2013) * Basis: * Method/Device: Multicorer with television (TVMUC) * Comment: rope length max. 1265m / FR2

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		Bienhold, Christina
2	Date/Time of event	Date/Time		Bienhold, Christina
3	Latitude of event	Latitude		Bienhold, Christina
4	Longitude of event	Longitude		Bienhold, Christina
5	Elevation of event	Elevation	m	Bienhold, Christina
6	DEPTH, sediment/rock	Depth sed	m	Bienhold, Christina		Geocode
7	Prokaryotes, abundance as single cells	Prok cell abund	10⁹ #/cm³	Bienhold, Christina	Acridine Orange Direct Counting (AODC)
8	Bacteria, cell biovolume	Bact cell biovol	µm³	Bienhold, Christina
9	Bacteria, biomass as carbon	Bact C	µg/cm³	Bienhold, Christina	Carbon biomass estimation (unspec.)
10	Comment	Comment		Bienhold, Christina

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Size:

76 data points

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