Bienhold, Christina (2019): Prokaryotic cell abundance and biomass measured during Maria S. Merian cruise MSM29 and Polarstern cruise PS85 to the Fram Strait [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.908130
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Abstract:
Prokaryotic cell abundances and biomass were determined in deep-sea sediment samples collected at the deep-sea LTER HAUSGARTEN, located in the Fram Strait. Sampling was performed during two cruises, i.e. RV Maria S. Merian MSM 29 in summer 2013 (23 June-12 July) and RV Polarstern PS85 in summer 2014 (6 June-3 July). Undisturbed sediment cores were collected with a TV-guided multicorer. Prokaryotic cell abundances were determined using acridine orange direct counts, following the procedure described in (Hoffmann et al., 2017; doi:10.3389/fmicb.2017.00266). For each sample two replicate filters were counted. Cell volumes were determined using a stage micrometer and converted to biomass using a conversion factor of 3 x 10^-13 g C /µm³ (Børsheim, K. Y., Bratbak, G., and Heldal, M. (1990). Enumeration and biomass estimation of planktonic bacteria and viruses by transmission electron microscopy. Applied and Environmental Microbiology 56, 352-356).
Keyword(s):
Supplement to:
Rossel, Pamela E; Bienhold, Christina; Hehemann, Laura; Dittmar, Thorsten; Boetius, Antje (2020): Molecular Composition of Dissolved Organic Matter in Sediment Porewater of the Arctic Deep-Sea Observatory HAUSGARTEN (Fram Strait). Frontiers in Marine Science, 7, 428, https://doi.org/10.3389/fmars.2020.00428
Project(s):
Funding:
Seventh Framework Programme (FP7), grant/award no. 294757: Assessment of bacterial life and matter cycling in deep-sea surface sediments
Coverage:
Median Latitude: 79.053645 * Median Longitude: 3.427493 * South-bound Latitude: 78.505670 * West-bound Longitude: -5.290500 * North-bound Latitude: 79.757830 * East-bound Longitude: 7.003500
Date/Time Start: 2013-06-26T01:11:00 * Date/Time End: 2014-06-25T16:59:00
Minimum DEPTH, sediment/rock: 0.005 m * Maximum DEPTH, sediment/rock: 0.005 m
Event(s):
MSM29_424-3 (HG_II) * Latitude Start: 79.132330 * Longitude Start: 4.911500 * Latitude End: 79.132000 * Longitude End: 4.912000 * Date/Time Start: 2013-06-26T01:11:00 * Date/Time End: 2013-06-26T02:19:59 * Elevation Start: -1506.3 m * Elevation End: -1506.3 m * Location: North Greenland Sea * Campaign: MSM29 (HAUSGARTEN 2013) * Basis: Maria S. Merian * Method/Device: Multicorer with television (TVMUC) * Comment: rope length max. 1485m / FR2
MSM29_425-3 (HG_I) * Latitude: 79.133330 * Longitude: 6.092500 * Date/Time: 2013-06-26T07:10:00 * Elevation: -1253.8 m * Location: North Greenland Sea * Campaign: MSM29 (HAUSGARTEN 2013) * Basis: Maria S. Merian * Method/Device: MultiCorer (MUC) * Comment: rope length max. 1240m / FR2/2
MSM29_427-2 (KH) * Latitude Start: 79.030000 * Longitude Start: 7.003500 * Latitude End: 79.030170 * Longitude End: 7.002670 * Date/Time Start: 2013-06-27T01:02:00 * Date/Time End: 2013-06-27T01:58:59 * Elevation Start: -1280.8 m * Elevation End: -1279.4 m * Location: North Greenland Sea * Campaign: MSM29 (HAUSGARTEN 2013) * Basis: Maria S. Merian * Method/Device: Multicorer with television (TVMUC) * Comment: rope length max. 1265m / FR2
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Bienhold, Christina | |||
| 2 | Date/Time of event | Date/Time | Bienhold, Christina | |||
| 3 | Latitude of event | Latitude | Bienhold, Christina | |||
| 4 | Longitude of event | Longitude | Bienhold, Christina | |||
| 5 | Elevation of event | Elevation | m | Bienhold, Christina | ||
| 6 | DEPTH, sediment/rock | Depth sed | m | Bienhold, Christina | Geocode | |
| 7 | Prokaryotes, abundance as single cells | Prok cell abund | 109 #/cm3 | Bienhold, Christina | Acridine Orange Direct Counting (AODC) | |
| 8 | Bacteria, cell biovolume | Bact cell biovol | µm3 | Bienhold, Christina | ||
| 9 | Bacteria, biomass as carbon | Bact C | µg/cm3 | Bienhold, Christina | Carbon biomass estimation (unspec.) | |
| 10 | Comment | Comment | Bienhold, Christina |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
76 data points
Data
| 1 Event | 2 Date/Time | 3 Latitude | 4 Longitude | 5 Elevation [m] | 6 Depth sed [m] | 7 Prok cell abund [109 #/cm3] | 8 Bact cell biovol [µm3] | 9 Bact C [µg/cm3] | 10 Comment |
|---|---|---|---|---|---|---|---|---|---|
| MSM29_427-2 | 2013-06-27T01:02 | 79.0300 | 7.0035 | -1281 | 0.005 | 1.40 | 0.168 | 70.64 | Hausgarten station KH |
| MSM29_425-3 | 2013-06-26T07:10 | 79.1333 | 6.0925 | -1254 | 0.005 | 1.67 | 0.141 | 70.50 | Hausgarten station HGI |
| MSM29_424-3 | 2013-06-26T01:11 | 79.1323 | 4.9115 | -1506 | 0.005 | 1.12 | 0.151 | 50.84 | Hausgarten station HGII |
| MSM29_432-2 | 2013-06-28T04:35 | 79.7182 | 4.5643 | -2745 | 0.005 | 1.62 | 0.146 | 70.99 | Hausgarten station N4 |
| MSM29_450-2 | 2013-07-08T05:05 | 78.9168 | 5.0050 | -2591 | 0.005 | 1.65 | 0.180 | 89.19 | Hausgarten station S1 |
| MSM29_443-2 | 2013-07-03T20:10 | 78.7778 | 5.2597 | -2465 | 0.005 | 1.83 | 0.224 | 122.59 | Hausgarten station S2 |
| MSM29_439-3 | 2013-07-01T08:09 | 78.6200 | 5.0168 | -2319 | 0.005 | 1.67 | 0.207 | 103.30 | Hausgarten station S3 |
| PS85/470-3 | 2014-06-24T23:34 | 79.1335 | 6.1065 | -1244 | 0.005 | 3.16 | 0.150 | 138.04 | Hausgarten station HGI |
| PS85/469-2 | 2014-06-24T19:18 | 79.1322 | 4.9062 | -1492 | 0.005 | 1.98 | 0.150 | 87.05 | Hausgarten station HGII |
| PS85/468-1 | 2014-06-24T15:43 | 79.1065 | 4.5850 | -1905 | 0.005 | 1.61 | 0.110 | 51.69 | Hausgarten station HGIII |
| PS85/460-4 | 2014-06-22T18:47 | 79.0652 | 4.1830 | -2403 | 0.005 | 1.65 | 0.140 | 67.08 | Hausgarten station HGIV |
| PS85/463-1 | 2014-06-23T13:58 | 79.0523 | 3.6728 | -3034 | 0.005 | 2.73 | 0.120 | 99.62 | Hausgarten station HGV |
| PS85/464-1 | 2014-06-23T16:31 | 79.0592 | 3.5728 | -3531 | 0.005 | 1.23 | 0.160 | 59.82 | Hausgarten station HGVI |
| PS85/465-4 | 2014-06-24T02:48 | 79.1583 | 2.8828 | -5525 | 0.005 | 1.42 | 0.140 | 57.84 | Hausgarten station HGIX |
| PS85/473-7 | 2014-06-25T16:59 | 79.7578 | 4.3767 | -2566 | 0.005 | 1.88 | 0.154 | 86.78 | Hausgarten station N4 |
| PS85/436-1 | 2014-06-17T14:30 | 78.9733 | -5.2905 | -1056 | 0.005 | 1.35 | 0.111 | 45.06 | Hausgarten station EGI |
| PS85/441-1 | 2014-06-18T03:18 | 78.9335 | -4.6503 | -1500 | 0.005 | 1.48 | 0.135 | 60.20 | Hausgarten station EGII |
| PS85/445-1 | 2014-06-19T02:02 | 78.8032 | -3.8753 | -1944 | 0.005 | 1.37 | 0.121 | 49.88 | Hausgarten station EGIII |
| PS85/454-3 | 2014-06-20T17:59 | 78.5057 | -2.8177 | -2558 | 0.005 | 2.24 | 0.133 | 89.88 | Hausgarten station EGIV |