Citation:

PANGAEA.905210 (dataset in review)

Name: CARD-FISH cell counts of Bacteria and Archaea in sediments of HAUSGARTEN, Fram Strait, from Polarstern cruise PS85
License: https://creativecommons.org/licenses/by/4.0/
Keywords: CARD-FISH; cell counts; Hausgarten

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Abstract:

We quantified Bacteria and Archaea, as well as 15 bacterial groups at various taxonomic ranks using catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH) in HAUSGARTEN surface sediments at stations HGI, HGIV, HGVI, and HGIX.

Keyword(s):

CARD-FISH; cell counts; Hausgarten

Related to:

Cardozo-Mino, Magda Guadalupe; Salman-Carvalho, Verena; Boetius, Antje; Fadeev, Eduard (2019): CARD-FISH cell counts of taxonomic bacterial and archaeal groups in Fram Strait during PS99.2. PANGAEA, https://doi.org/10.1594/PANGAEA.905212

Salman-Carvalho, Verena (2019): CARD-FISH counts of the water column and sediment at HAUSGARTEN, Fram Strait. PANGAEA, https://doi.org/10.1594/PANGAEA.903569

Further details:

Oligonucleotide probes and formamide concentration in HAUSGARTEN surface sediments at stations HGI, HGIV, HGVI, and HGIX

Coverage:

Median Latitude: 79.104042 * Median Longitude: 4.186290 * South-bound Latitude: 79.059170 * West-bound Longitude: 2.882830 * North-bound Latitude: 79.158330 * East-bound Longitude: 6.106500

Date/Time Start: 2014-06-22T18:47:00 * Date/Time End: 2014-06-24T23:34:00

Minimum DEPTH, sediment/rock: 0.005 m * Maximum DEPTH, sediment/rock: 0.005 m

Event(s):

PS85/460-4 (HGIV) * Latitude: 79.065170 * Longitude: 4.183000 * Date/Time: 2014-06-22T18:47:00 * Elevation: -2402.6 m * Location: North Greenland Sea * Campaign: (ARK-XXVIII/2) * Basis: * Method/Device: Multicorer with television (TVMUC)

PS85/464-1 (HGVI) * Latitude: 79.059170 * Longitude: 3.572830 * Date/Time: 2014-06-23T16:31:00 * Elevation: -3531.0 m * Location: North Greenland Sea * Campaign: (ARK-XXVIII/2) * Basis: * Method/Device: Multicorer with television (TVMUC)

PS85/465-4 (HGIX) * Latitude: 79.158330 * Longitude: 2.882830 * Date/Time: 2014-06-24T02:48:00 * Elevation: -5524.6 m * Location: North Greenland Sea * Campaign: (ARK-XXVIII/2) * Basis: * Method/Device: Multicorer with television (TVMUC)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		Salman-Carvalho, Verena
2	Optional event label	Event 2		Salman-Carvalho, Verena
3	Date/Time of event	Date/Time		Salman-Carvalho, Verena
4	Method/Device of event	Method/Device		Salman-Carvalho, Verena
5	Latitude of event	Latitude		Salman-Carvalho, Verena
6	Longitude of event	Longitude		Salman-Carvalho, Verena
7	Elevation of event	Elevation	m	Salman-Carvalho, Verena
8	Sample material	Samp mat		Salman-Carvalho, Verena
9	Depth, top/min	Depth top	m	Salman-Carvalho, Verena
10	Depth, bottom/max	Depth bot	m	Salman-Carvalho, Verena
11	DEPTH, sediment/rock	Depth sed	m	Salman-Carvalho, Verena		Geocode
12	Microbial abundance, cells	Microbial abund	#/ml	Salman-Carvalho, Verena	Fluorescence determination of DAPI stain
13	Bacteria, targed with EUB338(I-III) oligonucleotide FISH-probe	EUB338 I-III	#/ml	Salman-Carvalho, Verena	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
14	Archaea, targed with ARCH915 oligonucleotide FISH-probe	ARCH915	#/ml	Salman-Carvalho, Verena	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
15	Gammaproteobacteria, targeted with Gam42a oligonucleotide FISH-probe	Gam42a	#/ml	Salman-Carvalho, Verena	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
16	Deltaproteobacteria, targeted with the DELTA495 mix oligonucleotide FISH-probes	DELTA495 mix	#/ml	Salman-Carvalho, Verena	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
17	Alphaproteobacteria, targeted with ALF968 oligonucleotides FISH-probe	ALF968	#/ml	Salman-Carvalho, Verena	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
18	Chloroflexi, targeted with CFX1223 and GNSB941 oligonucleotides FISH-probe	CFX1223 + GNSB941	#/ml	Salman-Carvalho, Verena	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
19	Actinobacteria, targeted with HGC236 oligonucleotides FISH-probe	HGC236	#/ml	Salman-Carvalho, Verena	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
20	Bacteroidetes, targeted with CF968 oligonucleotides FISH-probe	CF968	#/ml	Salman-Carvalho, Verena	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
21	Phycisphaerae, targeted with PHYC309 oligonucleotides FISH-probe	PHYC309	#/ml	Salman-Carvalho, Verena	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
22	OM182 clade, targeted with OM182-707 oligonucleotides FISH-probe	OM182-707	#/ml	Salman-Carvalho, Verena	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
23	NB1j clade, targed with the NB1j-428, NB1j-667, NB1j-823 and NB1j-843 oligonucleotide FISH-probe	NB1j mix	#/ml	Salman-Carvalho, Verena	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
24	Actinomarinales, targeted with OM1-952 and OM1-389 oligonucleotides FISH-probe	OM1	#/ml	Salman-Carvalho, Verena	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
25	Flavobacteriales, targeted with CFB563 oligonucleotides FISH-probe	CFB563	#/ml	Salman-Carvalho, Verena	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
26	Cytophagales, targeted with CF319b oligonucleotides FISH-probe	CF319b	#/ml	Salman-Carvalho, Verena	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
27	SAR202 clade, targeted with SAR202-312R oligonucleotide FISH-probe	SAR202-312R	#/ml	Salman-Carvalho, Verena	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
28	Colwellia and Alteromonas, targeted with ALT1413 oligonucleiotide FISH-Probe	ALT1413	#/ml	Salman-Carvalho, Verena	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
29	JTB255, targeted with JTB819a, JTB819b and JTB897 oligonucleotide FISH-probe	JTB255 mix	#/ml	Salman-Carvalho, Verena	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)

Size:

84 data points

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