Carini, Alessia; Koudelka, Tomas; Tholey, Andreas; Appel, Esther; Gorb, Stanislav N; Melzner, Frank; Ramesh, Kirti (2019): Experiment 2: blue mussel larval shell cultures for shell proteomic analysis: Bicinchoninic acid (BCA) assay [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.901794, In supplement to: Carini, A et al. (2019): Proteomic investigation of the blue mussel larval shell organic matrix. Journal of Structural Biology, 208(3), 107385, https://doi.org/10.1016/j.jsb.2019.09.002
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Abstract:
Output of the bicinchoninic acid assay (BCA) measuring the total protein content in the pooled larval shell samples (F1= family 1, F2= family 2, F3= family 3).
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Species | Species | Carini, Alessia | |||
| 2 | Sample type | Samp type | Carini, Alessia | |||
| 3 | Sample ID | Sample ID | Carini, Alessia | |||
| 4 | Proteins | Protein | ng | Carini, Alessia | known concentration | |
| 5 | Blank | Blank | ng | Carini, Alessia | Blank 562 | |
| 6 | Proteins | Protein | ng | Carini, Alessia | measured concentration |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
86 data points
Data
| 1 Species | 2 Samp type | 3 Sample ID | 4 Protein [ng] (known concentration) | 5 Blank [ng] (Blank 562) | 6 Protein [ng] (measured concentration) |
|---|---|---|---|---|---|
| Baltic Mytilus edulis-like | prodissoconch I larvae (at 48 hours post fertilization) | F1 | 0.033 | 31.5 | |
| Baltic Mytilus edulis-like | prodissoconch I larvae (at 48 hours post fertilization) | F1 | 0.064 | 55.8 | |
| Baltic Mytilus edulis-like | prodissoconch I larvae (at 48 hours post fertilization) | F2 | 0.052 | 46.4 | |
| Baltic Mytilus edulis-like | prodissoconch I larvae (at 48 hours post fertilization) | F2 | 0.061 | 53.5 | |
| Baltic Mytilus edulis-like | prodissoconch I larvae (at 48 hours post fertilization) | F3 | 0.077 | 66.0 | |
| Baltic Mytilus edulis-like | prodissoconch I larvae (at 48 hours post fertilization) | F3 | 0.094 | 79.4 | |
| standard 4 | 31.3 | 0.033 | 31.5 | ||
| standard 4 | 31.3 | 0.033 | 31.5 | ||
| standard 8 | 62.5 | 0.072 | 62.1 | ||
| standard 8 | 62.5 | 0.064 | 55.8 | ||
| standard 10 | 125.0 | 0.150 | 123.9 | ||
| standard 10 | 125.0 | 0.157 | 129.5 | ||
| standard 11 | 250.0 | 0.312 | 255.4 | ||
| standard 11 | 250.0 | 0.329 | 269.5 | ||
| standard 12 | 500.0 | 0.614 | 513.3 | ||
| standard 12 | 500.0 | 0.614 | 513.3 | ||
| standard 14 | 1000.0 | 1.103 | 975.2 | ||
| standard 14 | 1000.0 | 1.076 | 947.9 | ||
| standard 16 | 2000.0 | 1.963 | 2019.0 | ||
| standard 16 | 2000.0 | 1.963 | 2019.0 |