Franco-Santos, Rita Melo; Auel, Holger; Boersma, Maarten; De Troch, Marleen; Graeve, Martin; Meunier, Cédric Léo; Niehoff, Barbara (2019): Fatty acid contents for the diatom Conticribra weissflogii [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.900108, In supplement to: Franco-Santos, RM et al. (2019): You are not always what you eat—Fatty acid bioconversion and lipid homeostasis in the larvae of the sand mason worm Lanice conchilega. PLoS ONE, 14(6), e0218015, https://doi.org/10.1371/journal.pone.0218015
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Abstract:
The larvae of the reef-building polychaete Lanice conchilega can make up to 15% of the summer zooplankton biomass in the North Sea. Despite their importance for reef maintenance (which positively affects the benthic community), little is known about the trophic ecology of this meroplanktonic larva. Qualitative and quantitative estimates of carbon (C) transfer between trophic levels and of fatty acid (FA)-specific assimilation, biosynthesis, and bioconversion can be obtained by compound-specific stable isotope analysis of FA. The present work tested the hypothesis that the concept of fatty acid trophic markers (FATM), widely used for studies on holoplankton with intermediate to high lipid contents, is also applicable to lipid-poor organisms such as meroplanktonic larvae. The incorporation of isotopically-enriched dietary C by L. conchilega larvae was traced, and lipid assimilation did not follow FA-specific relative availabilities in the diet. Furthermore, FAs that were unavailable in the diet, such as 22:5(n-3), were recorded in L. conchilega, suggesting their bioconversion by the larvae. The results indicate that L. conchilega larvae preferentially assimilate certain FAs and regulate their FA composition (lipid homeostasis) independently of that of their diet. Their quasi-homeostatic response to dietary FA availability could imply that the concept of FATM has limited application in lipid-poor organisms such as L. conchilega larvae.
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| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Species | Species | Franco-Santos, Rita Melo | |||
| 2 | Replicate | Repl | Franco-Santos, Rita Melo | |||
| 3 | Fatty acid, common name | FA common | Franco-Santos, Rita Melo | |||
| 4 | Fatty acid, n-x nomenclature | FA A:B(n-x) | Franco-Santos, Rita Melo | A: number of carbon atoms: B: number of double bonds; (n-X): position of the double bond closest to the terminal methyl group | ||
| 5 | Fatty acid, IUPAC nomenclature | FA IUPAC | Franco-Santos, Rita Melo | |||
| 6 | Fatty acids, δ13C | FA δ13C | ‰ PDB | Franco-Santos, Rita Melo | δ 13C (‰) = [((13Csample/12Csample)/(13CVPDBstandard/12CVPDBstandard)) -1] * 1000 | |
| 7 | Atom percent | At perc | % | Franco-Santos, Rita Melo | Atom% = ((13Csample/12Csample)/((13Csample/12Csample)+1)) * 100 | |
| 8 | Fatty acid content | FA con | µg | Franco-Santos, Rita Melo | in sample | |
| 9 | Fatty acid as percentage of total fatty acids | FA | % | Franco-Santos, Rita Melo | %TFA = (mass FA sample * 100) / ∑(mass all FAs sample) | |
| 10 | Fatty acid per cell | FA/cell | pg/# | Franco-Santos, Rita Melo | ||
| 11 | Fatty acids, total | TFA | µg/l | Franco-Santos, Rita Melo | Mass FA culture | |
| 12 | Carbon, number of atoms | C atoms | # | Franco-Santos, Rita Melo | Number of atoms in the FA molecule (CFA) | |
| 13 | Number of insaturations | N insatur | # | Franco-Santos, Rita Melo | Number of insaturations in the FA molecule (M) | |
| 14 | Hydrogen, number of atoms | H atoms | # | Franco-Santos, Rita Melo | Number of atoms in the FA molecule (HFA,=2CFA-2M) | |
| 15 | Carbon, number of atoms | C atoms | # | Franco-Santos, Rita Melo | Number of atoms in the FAME molecule (CFAME) | |
| 16 | Number of insaturations | N insatur | # | Franco-Santos, Rita Melo | Number of insaturations in the FAME molecule (M) | |
| 17 | Hydrogen, number of atoms | H atoms | # | Franco-Santos, Rita Melo | Number of atoms in the FAME molecule (HFAME, =2CFAME-2M) | |
| 18 | Oxygen, number of atoms | O atoms | # | Franco-Santos, Rita Melo | Number of atoms in the FAME molecule | |
| 19 | Atomic weight | Atomic weight | u | Franco-Santos, Rita Melo | 13C (A13C) | |
| 20 | Atomic weight | Atomic weight | u | Franco-Santos, Rita Melo | 12C (A12C) | |
| 21 | Atomic weight | Atomic weight | u | Franco-Santos, Rita Melo | H (AH) | |
| 22 | Atomic weight | Atomic weight | u | Franco-Santos, Rita Melo | O (AO) | |
| 23 | Fatty acid as carbon | FA C | µg/l | Franco-Santos, Rita Melo | Cmass = (((atom% / 100*A13C) + ((100-atom%) / 100* A12C)) * CFA * FAmass) / (((atom% / 100*A13C) + ((100-atom%) / 100* A12C)) * CFA) + A12C + (HFAME * AH) + (OFAME * AO) | |
| 24 | Fatty acid as carbon per cell | FA C/cell | pg/# | Franco-Santos, Rita Melo | = Cmass / 8000000 * 1000000 |
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Creative Commons Attribution 4.0 International (CC-BY-4.0)
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1669 data points