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Franco-Santos, Rita Melo; Auel, Holger; Boersma, Maarten; De Troch, Marleen; Graeve, Martin; Meunier, Cédric Léo; Niehoff, Barbara (2019): Fatty acid contents for the diatom Conticribra weissflogii [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.900108, In supplement to: Franco-Santos, RM et al. (2019): You are not always what you eat—Fatty acid bioconversion and lipid homeostasis in the larvae of the sand mason worm Lanice conchilega. PLoS ONE, 14(6), e0218015, https://doi.org/10.1371/journal.pone.0218015

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Abstract:
The larvae of the reef-building polychaete Lanice conchilega can make up to 15% of the summer zooplankton biomass in the North Sea. Despite their importance for reef maintenance (which positively affects the benthic community), little is known about the trophic ecology of this meroplanktonic larva. Qualitative and quantitative estimates of carbon (C) transfer between trophic levels and of fatty acid (FA)-specific assimilation, biosynthesis, and bioconversion can be obtained by compound-specific stable isotope analysis of FA. The present work tested the hypothesis that the concept of fatty acid trophic markers (FATM), widely used for studies on holoplankton with intermediate to high lipid contents, is also applicable to lipid-poor organisms such as meroplanktonic larvae. The incorporation of isotopically-enriched dietary C by L. conchilega larvae was traced, and lipid assimilation did not follow FA-specific relative availabilities in the diet. Furthermore, FAs that were unavailable in the diet, such as 22:5(n-3), were recorded in L. conchilega, suggesting their bioconversion by the larvae. The results indicate that L. conchilega larvae preferentially assimilate certain FAs and regulate their FA composition (lipid homeostasis) independently of that of their diet. Their quasi-homeostatic response to dietary FA availability could imply that the concept of FATM has limited application in lipid-poor organisms such as L. conchilega larvae.
Keyword(s):
assimilation; bioconversion; compound-specific stable isotope analysis; fatty acid trophic markers; lipid-poor species; meroplanktonic larvae; polychaete
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
SpeciesSpeciesFranco-Santos, Rita Melo
ReplicateReplFranco-Santos, Rita Melo
Fatty acid, common nameFA commonFranco-Santos, Rita Melo
Fatty acid, n-x nomenclatureFA A:B(n-x)Franco-Santos, Rita MeloA: number of carbon atoms: B: number of double bonds; (n-X): position of the double bond closest to the terminal methyl group
Fatty acid, IUPAC nomenclatureFA IUPACFranco-Santos, Rita Melo
Fatty acids, δ13CFA δ13C‰ PDBFranco-Santos, Rita Meloδ 13C (‰) = [((13Csample/12Csample)/(13CVPDBstandard/12CVPDBstandard)) -1] * 1000
Atom percentAt perc%Franco-Santos, Rita MeloAtom% = ((13Csample/12Csample)/((13Csample/12Csample)+1)) * 100
Fatty acid contentFA conµgFranco-Santos, Rita Meloin sample
Fatty acid as percentage of total fatty acidsFA%Franco-Santos, Rita Melo%TFA = (mass FA sample * 100) / ∑(mass all FAs sample)
10 Fatty acid per cellFA/cellpg/#Franco-Santos, Rita Melo
11 Fatty acids, totalTFAµg/lFranco-Santos, Rita MeloMass FA culture
12 Carbon, number of atomsC atoms#Franco-Santos, Rita MeloNumber of atoms in the FA molecule (CFA)
13 Number of insaturationsN insatur#Franco-Santos, Rita MeloNumber of insaturations in the FA molecule (M)
14 Hydrogen, number of atomsH atoms#Franco-Santos, Rita MeloNumber of atoms in the FA molecule (HFA,=2CFA-2M)
15 Carbon, number of atomsC atoms#Franco-Santos, Rita MeloNumber of atoms in the FAME molecule (CFAME)
16 Number of insaturationsN insatur#Franco-Santos, Rita MeloNumber of insaturations in the FAME molecule (M)
17 Hydrogen, number of atomsH atoms#Franco-Santos, Rita MeloNumber of atoms in the FAME molecule (HFAME, =2CFAME-2M)
18 Oxygen, number of atomsO atoms#Franco-Santos, Rita MeloNumber of atoms in the FAME molecule
19 Atomic weightAtomic weightuFranco-Santos, Rita Melo13C (A13C)
20 Atomic weightAtomic weightuFranco-Santos, Rita Melo12C (A12C)
21 Atomic weightAtomic weightuFranco-Santos, Rita MeloH (AH)
22 Atomic weightAtomic weightuFranco-Santos, Rita MeloO (AO)
23 Fatty acid as carbonFA Cµg/lFranco-Santos, Rita MeloCmass = (((atom% / 100*A13C) + ((100-atom%) / 100* A12C)) * CFA * FAmass) / (((atom% / 100*A13C) + ((100-atom%) / 100* A12C)) * CFA) + A12C + (HFAME * AH) + (OFAME * AO)
24 Fatty acid as carbon per cellFA C/cellpg/#Franco-Santos, Rita Melo= Cmass / 8000000 * 1000000
Size:
1669 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Species

Repl

FA common

FA A:B(n-x)
(A: number of carbon atoms: B:...)

FA IUPAC

FA δ13C [‰ PDB]
(δ 13C (‰) = [((13Csample/12Cs...)

At perc [%]
(Atom% = ((13Csample/12Csample...)

FA con [µg]
(in sample)

FA [%]
(%TFA = (mass FA sample * 100)...)
10 
FA/cell [pg/#]
11 
TFA [µg/l]
(Mass FA culture)
12 
C atoms [#]
(Number of atoms in the FA mol...)
13 
N insatur [#]
(Number of insaturations in th...)
14 
H atoms [#]
(Number of atoms in the FA mol...)
15 
C atoms [#]
(Number of atoms in the FAME m...)
16 
N insatur [#]
(Number of insaturations in th...)
17 
H atoms [#]
(Number of atoms in the FAME m...)
18 
O atoms [#]
(Number of atoms in the FAME m...)
19 
Atomic weight [u]
(13C (A13C))
20 
Atomic weight [u]
(12C (A12C))
21 
Atomic weight [u]
(H (AH))
22 
Atomic weight [u]
(O (AO))
23 
FA C [µg/l]
(Cmass = (((atom% / 100*A13C) ...)
24 
FA C/cell [pg/#]
(= Cmass / 8000000 * 1000000)
Conticribra weissflogii1Myristic acid14:00Tetradecanoic acid28274.106.06271.29.3261402815030213.003355121.007915.99946.4750.81
Conticribra weissflogii1i-15:029044.180.49910.10.7681503016032213.003355121.007915.99940.5400.07
Conticribra weissflogii1Pentadecanoic acid15:0027894.060.80710.21.2411503016032213.003355121.007915.99940.8730.11
Conticribra weissflogii1Palmitic acid16:00Hexadecanoic acid27394.0112.208142.318.7811603217034213.003355121.007915.999413.3551.67
Conticribra weissflogii1Palmitoleic acid16:1(n-7)(Z)-hexadec-9-enoic acid28384.1114.000162.721.5381613017132213.003355121.007915.999415.4311.93
Conticribra weissflogii1Palmitovaccenic acid16:1(n-5)(11Z)-hexadec-11-enoic acid26053.871.04310.21.6041613017132213.003355121.007915.99941.1490.14
Conticribra weissflogii116:2(n-4)28284.107.16081.411.0161622817230213.003355121.007915.99947.9520.99
Conticribra weissflogii116:3(n-4)28274.1014.909172.922.9371632617328213.003355121.007915.999416.6832.09
Conticribra weissflogii1Stearic acid18:00Octadecanoic acid9502.130.45310.10.6961803619038213.003355121.007915.99940.5050.06
Conticribra weissflogii1Oleic acid18:1(n-9)(Z)-octadec-9-enoic acid23923.650.34800.10.5351813419136213.003355121.007915.99940.3910.05
Conticribra weissflogii1Vaccenic acid18:1(n-7)(E)-Octadec-11-enoic acid23283.590.36100.10.5561813419136213.003355121.007915.99940.4060.05
Conticribra weissflogii1Linoleic acid18:2(n-6)(9Z,12Z)-octadeca-9,12-dienoic acid27814.060.77210.11.1881823219234213.003355121.007915.99940.8730.11
Conticribra weissflogii1γ-Linolenic acid18:3(n-6)28814.160.44110.10.6791833019332213.003355121.007915.99940.5020.06
Conticribra weissflogii1Stearidonic acid18:4(n-3)(6Z,9Z,12Z,15Z)-6,9,12,15-octadecatetraenoic acid28704.154.62050.97.1071842819430213.003355121.007915.99945.2940.66
Conticribra weissflogii1Arachidonic acid20:4(n-6)(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoic acid27053.980.22000.00.3392043221434213.003355121.007915.99940.2560.03
Conticribra weissflogii1Eicosatetraenoic acid20:4(n-3)24073.670.34800.10.5352043221434213.003355121.007915.99940.4040.05
Conticribra weissflogii1Eicosapentaenoic acid20:5(n-3)(5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoic acid28464.1218.027213.527.7332053021532213.003355121.007915.999421.0642.63
Conticribra weissflogii1Docosahexaenoic acid22:6(n-3)(4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoic acid28684.153.80740.75.8572263223634213.003355121.007915.99944.5210.57
Conticribra weissflogii2Myristic acid14:00Tetradecanoic acid17442.983.71260.75.7101402815030213.003355121.007915.99943.9630.50
Conticribra weissflogii2i-15:011042.300.52810.10.8121503016032213.003355121.007915.99940.5710.07
Conticribra weissflogii2Pentadecanoic acid15:0017202.950.61810.10.9511503016032213.003355121.007915.99940.6690.08
Conticribra weissflogii2Palmitic acid16:00Hexadecanoic acid16732.908.809131.713.5531603217034213.003355121.007915.99949.6351.20
Conticribra weissflogii2Palmitoleic acid16:1(n-7)(Z)-hexadec-9-enoic acid17362.979.651141.914.8471613017132213.003355121.007915.999410.6341.33
Conticribra weissflogii2Palmitovaccenic acid16:1(n-5)(11Z)-hexadec-11-enoic acid15652.790.76610.11.1781613017132213.003355121.007915.99940.8440.11
Conticribra weissflogii216:2(n-4)17592.995.71891.18.7971622817230213.003355121.007915.99946.3490.79
Conticribra weissflogii216:3(n-4)17643.0012.253182.418.8501632617328213.003355121.007915.999413.7071.71
Conticribra weissflogii2Stearic acid18:00Octadecanoic acid4501.600.50310.10.7731803619038213.003355121.007915.99940.5600.07
Conticribra weissflogii2Oleic acid18:1(n-9)(Z)-octadec-9-enoic acid13602.570.32500.10.5011813419136213.003355121.007915.99940.3650.05
Conticribra weissflogii2Vaccenic acid18:1(n-7)(E)-Octadec-11-enoic acid14142.630.51710.10.7951813419136213.003355121.007915.99940.5800.07
Conticribra weissflogii2Linoleic acid18:2(n-6)(9Z,12Z)-octadeca-9,12-dienoic acid17392.970.69810.11.0741823219234213.003355121.007915.99940.7890.10
Conticribra weissflogii2γ-Linolenic acid18:3(n-6)18063.040.47510.10.7311833019332213.003355121.007915.99940.5410.07
Conticribra weissflogii2Stearidonic acid18:4(n-3)(6Z,9Z,12Z,15Z)-6,9,12,15-octadecatetraenoic acid17693.002.77540.54.2691842819430213.003355121.007915.99943.1790.40
Conticribra weissflogii2Arachidonic acid20:4(n-6)(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoic acid16942.920.28400.10.4382043221434213.003355121.007915.99940.3300.04
Conticribra weissflogii2Eicosapentaenoic acid20:5(n-3)(5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoic acid17592.9914.793222.822.7582053021532213.003355121.007915.999417.2812.16
Conticribra weissflogii2Docosahexaenoic acid22:6(n-3)(4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoic acid17793.014.15660.86.3942263223634213.003355121.007915.99944.9350.62
Conticribra weissflogii4Myristic acid14:00Tetradecanoic acid24233.695.28671.08.1321402815030213.003355121.007915.99945.6450.71
Conticribra weissflogii4i-15:025083.770.52510.10.8071503016032213.003355121.007915.99940.5680.07
Conticribra weissflogii4Pentadecanoic acid15:0023693.630.69310.11.0661503016032213.003355121.007915.99940.7500.09
Conticribra weissflogii4Palmitic acid16:00Hexadecanoic acid23053.5610.223132.015.7271603217034213.003355121.007915.999411.1821.40
Conticribra weissflogii4Palmitoleic acid16:1(n-7)(Z)-hexadec-9-enoic acid24373.7010.301132.015.8471613017132213.003355121.007915.999411.3521.42
Conticribra weissflogii4Palmitovaccenic acid16:1(n-5)(11Z)-hexadec-11-enoic acid21243.370.81510.21.2531613017132213.003355121.007915.99940.8980.11
Conticribra weissflogii416:2(n-4)24363.705.94481.19.1451622817230213.003355121.007915.99946.6010.83
Conticribra weissflogii416:3(n-4)24543.7216.049213.124.6911632617328213.003355121.007915.999417.9572.24
Conticribra weissflogii4Stearic acid18:00Octadecanoic acid4811.630.42910.10.6591803619038213.003355121.007915.99940.4780.06
Conticribra weissflogii4Oleic acid18:1(n-9)(Z)-octadec-9-enoic acid16622.890.31500.10.4851813419136213.003355121.007915.99940.3540.04
Conticribra weissflogii4Vaccenic acid18:1(n-7)(E)-Octadec-11-enoic acid20043.250.43910.10.6761813419136213.003355121.007915.99940.4930.06
Conticribra weissflogii4Linoleic acid18:2(n-6)(9Z,12Z)-octadeca-9,12-dienoic acid23903.650.80510.21.2381823219234213.003355121.007915.99940.9100.11
Conticribra weissflogii4γ-Linolenic acid18:3(n-6)24533.720.44610.10.6871833019332213.003355121.007915.99940.5080.06
Conticribra weissflogii4Stearidonic acid18:4(n-3)(6Z,9Z,12Z,15Z)-6,9,12,15-octadecatetraenoic acid24823.753.16640.64.8701842819430213.003355121.007915.99943.6270.45
Conticribra weissflogii4Arachidonic acid20:4(n-6)(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoic acid22993.560.28600.10.4412043221434213.003355121.007915.99940.3330.04
Conticribra weissflogii4Eicosatetraenoic acid20:4(n-3)19443.190.29700.10.4572043221434213.003355121.007915.99940.3440.04
Conticribra weissflogii4Eicosapentaenoic acid20:5(n-3)(5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoic acid24693.7317.332223.326.6652053021532213.003355121.007915.999420.2512.53
Conticribra weissflogii4Docosahexaenoic acid22:6(n-3)(4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoic acid24823.754.21250.86.4802263223634213.003355121.007915.99945.0010.63
Conticribra weissflogii5Myristic acid14:00Tetradecanoic acid28274.104.46760.96.8731402815030213.003355121.007915.99944.7720.60
Conticribra weissflogii5i-15:029044.180.42110.10.6481503016032213.003355121.007915.99940.4550.06
Conticribra weissflogii5Pentadecanoic acid15:0027894.060.66210.11.0191503016032213.003355121.007915.99940.7170.09
Conticribra weissflogii5Palmitic acid16:00Hexadecanoic acid27394.019.589131.814.7531603217034213.003355121.007915.999410.4911.31
Conticribra weissflogii5Palmitoleic acid16:1(n-7)(Z)-hexadec-9-enoic acid28384.1110.164142.015.6361613017132213.003355121.007915.999411.2031.40
Conticribra weissflogii5Palmitovaccenic acid16:1(n-5)(11Z)-hexadec-11-enoic acid26053.870.75210.11.1571613017132213.003355121.007915.99940.8290.10
Conticribra weissflogii516:2(n-4)28284.105.88981.19.0601622817230213.003355121.007915.99946.5400.82
Conticribra weissflogii516:3(n-4)28274.1014.380202.822.1231632617328213.003355121.007915.999416.0912.01
Conticribra weissflogii5Stearic acid18:00Octadecanoic acid9502.130.32200.10.4951803619038213.003355121.007915.99940.3590.04
Conticribra weissflogii5Oleic acid18:1(n-9)(Z)-octadec-9-enoic acid23923.650.25200.00.3871813419136213.003355121.007915.99940.2830.04
Conticribra weissflogii5Vaccenic acid18:1(n-7)(E)-Octadec-11-enoic acid23283.590.37210.10.5721813419136213.003355121.007915.99940.4170.05
Conticribra weissflogii5Linoleic acid18:2(n-6)(9Z,12Z)-octadeca-9,12-dienoic acid27814.060.77610.11.1951823219234213.003355121.007915.99940.8780.11
Conticribra weissflogii5γ-Linolenic acid18:3(n-6)28814.160.31200.10.4801833019332213.003355121.007915.99940.3550.04
Conticribra weissflogii5Stearidonic acid18:4(n-3)(6Z,9Z,12Z,15Z)-6,9,12,15-octadecatetraenoic acid28704.152.81840.54.3361842819430213.003355121.007915.99943.2300.40
Conticribra weissflogii5Arachidonic acid20:4(n-6)(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoic acid27053.980.21800.00.3352043221434213.003355121.007915.99940.2530.03
Conticribra weissflogii5Eicosatetraenoic acid20:4(n-3)24073.670.17100.00.2642043221434213.003355121.007915.99940.1990.02
Conticribra weissflogii5Eicosapentaenoic acid20:5(n-3)(5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoic acid28464.1216.138223.124.8282053021532213.003355121.007915.999418.8572.36
Conticribra weissflogii5Docosahexaenoic acid22:6(n-3)(4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoic acid28684.154.12460.86.3452263223634213.003355121.007915.99944.8980.61